Hypothesis definition and example

Hypothesis n., plural: hypotheses [/haɪˈpɑːθəsɪs/] Definition: Testable scientific prediction

Table of Contents

What Is Hypothesis?

A scientific hypothesis is a foundational element of the scientific method . It’s a testable statement proposing a potential explanation for natural phenomena. The term hypothesis means “little theory” . A hypothesis is a short statement that can be tested and gives a possible reason for a phenomenon or a possible link between two variables . In the setting of scientific research, a hypothesis is a tentative explanation or statement that can be proven wrong and is used to guide experiments and empirical research.

What is Hypothesis

It is an important part of the scientific method because it gives a basis for planning tests, gathering data, and judging evidence to see if it is true and could help us understand how natural things work. Several hypotheses can be tested in the real world, and the results of careful and systematic observation and analysis can be used to support, reject, or improve them.

Researchers and scientists often use the word hypothesis to refer to this educated guess . These hypotheses are firmly established based on scientific principles and the rigorous testing of new technology and experiments .

For example, in astrophysics, the Big Bang Theory is a working hypothesis that explains the origins of the universe and considers it as a natural phenomenon. It is among the most prominent scientific hypotheses in the field.

“The scientific method: steps, terms, and examples” by Scishow:

Biology definition: A hypothesis  is a supposition or tentative explanation for (a group of) phenomena, (a set of) facts, or a scientific inquiry that may be tested, verified or answered by further investigation or methodological experiment. It is like a scientific guess . It’s an idea or prediction that scientists make before they do experiments. They use it to guess what might happen and then test it to see if they were right. It’s like a smart guess that helps them learn new things. A scientific hypothesis that has been verified through scientific experiment and research may well be considered a scientific theory .

Etymology: The word “hypothesis” comes from the Greek word “hupothesis,” which means “a basis” or “a supposition.” It combines “hupo” (under) and “thesis” (placing). Synonym:   proposition; assumption; conjecture; postulate Compare:   theory See also: null hypothesis

Characteristics Of Hypothesis

A useful hypothesis must have the following qualities:

  • It should never be written as a question.
  • You should be able to test it in the real world to see if it’s right or wrong.
  • It needs to be clear and exact.
  • It should list the factors that will be used to figure out the relationship.
  • It should only talk about one thing. You can make a theory in either a descriptive or form of relationship.
  • It shouldn’t go against any natural rule that everyone knows is true. Verification will be done well with the tools and methods that are available.
  • It should be written in as simple a way as possible so that everyone can understand it.
  • It must explain what happened to make an answer necessary.
  • It should be testable in a fair amount of time.
  • It shouldn’t say different things.

Sources Of Hypothesis

Sources of hypothesis are:

  • Patterns of similarity between the phenomenon under investigation and existing hypotheses.
  • Insights derived from prior research, concurrent observations, and insights from opposing perspectives.
  • The formulations are derived from accepted scientific theories and proposed by researchers.
  • In research, it’s essential to consider hypothesis as different subject areas may require various hypotheses (plural form of hypothesis). Researchers also establish a significance level to determine the strength of evidence supporting a hypothesis.
  • Individual cognitive processes also contribute to the formation of hypotheses.

One hypothesis is a tentative explanation for an observation or phenomenon. It is based on prior knowledge and understanding of the world, and it can be tested by gathering and analyzing data. Observed facts are the data that are collected to test a hypothesis. They can support or refute the hypothesis.

For example, the hypothesis that “eating more fruits and vegetables will improve your health” can be tested by gathering data on the health of people who eat different amounts of fruits and vegetables. If the people who eat more fruits and vegetables are healthier than those who eat less fruits and vegetables, then the hypothesis is supported.

Hypotheses are essential for scientific inquiry. They help scientists to focus their research, to design experiments, and to interpret their results. They are also essential for the development of scientific theories.

Types Of Hypothesis

In research, you typically encounter two types of hypothesis: the alternative hypothesis (which proposes a relationship between variables) and the null hypothesis (which suggests no relationship).

Hypothesis testing

Simple Hypothesis

It illustrates the association between one dependent variable and one independent variable. For instance, if you consume more vegetables, you will lose weight more quickly. Here, increasing vegetable consumption is the independent variable, while weight loss is the dependent variable.

Complex Hypothesis

It exhibits the relationship between at least two dependent variables and at least two independent variables. Eating more vegetables and fruits results in weight loss, radiant skin, and a decreased risk of numerous diseases, including heart disease.

Directional Hypothesis

It shows that a researcher wants to reach a certain goal. The way the factors are related can also tell us about their nature. For example, four-year-old children who eat well over a time of five years have a higher IQ than children who don’t eat well. This shows what happened and how it happened.

Non-directional Hypothesis

When there is no theory involved, it is used. It is a statement that there is a connection between two variables, but it doesn’t say what that relationship is or which way it goes.

Null Hypothesis

It says something that goes against the theory. It’s a statement that says something is not true, and there is no link between the independent and dependent factors. “H 0 ” represents the null hypothesis.

Associative and Causal Hypothesis

When a change in one variable causes a change in the other variable, this is called the associative hypothesis . The causal hypothesis, on the other hand, says that there is a cause-and-effect relationship between two or more factors.

Examples Of Hypothesis

Examples of simple hypotheses:

  • Students who consume breakfast before taking a math test will have a better overall performance than students who do not consume breakfast.
  • Students who experience test anxiety before an English examination will get lower scores than students who do not experience test anxiety.
  • Motorists who talk on the phone while driving will be more likely to make errors on a driving course than those who do not talk on the phone, is a statement that suggests that drivers who talk on the phone while driving are more likely to make mistakes.

Examples of a complex hypothesis:

  • Individuals who consume a lot of sugar and don’t get much exercise are at an increased risk of developing depression.
  • Younger people who are routinely exposed to green, outdoor areas have better subjective well-being than older adults who have limited exposure to green spaces, according to a new study.
  • Increased levels of air pollution led to higher rates of respiratory illnesses, which in turn resulted in increased costs for healthcare for the affected communities.

Examples of Directional Hypothesis:

  • The crop yield will go up a lot if the amount of fertilizer is increased.
  • Patients who have surgery and are exposed to more stress will need more time to get better.
  • Increasing the frequency of brand advertising on social media will lead to a significant increase in brand awareness among the target audience.

Examples of Non-Directional Hypothesis (or Two-Tailed Hypothesis):

  • The test scores of two groups of students are very different from each other.
  • There is a link between gender and being happy at work.
  • There is a correlation between the amount of caffeine an individual consumes and the speed with which they react.

Examples of a null hypothesis:

  • Children who receive a new reading intervention will have scores that are different than students who do not receive the intervention.
  • The results of a memory recall test will not reveal any significant gap in performance between children and adults.
  • There is not a significant relationship between the number of hours spent playing video games and academic performance.

Examples of Associative Hypothesis:

  • There is a link between how many hours you spend studying and how well you do in school.
  • Drinking sugary drinks is bad for your health as a whole.
  • There is an association between socioeconomic status and access to quality healthcare services in urban neighborhoods.

Functions Of Hypothesis

The research issue can be understood better with the help of a hypothesis, which is why developing one is crucial. The following are some of the specific roles that a hypothesis plays: (Rashid, Apr 20, 2022)

  • A hypothesis gives a study a point of concentration. It enlightens us as to the specific characteristics of a study subject we need to look into.
  • It instructs us on what data to acquire as well as what data we should not collect, giving the study a focal point .
  • The development of a hypothesis improves objectivity since it enables the establishment of a focal point.
  • A hypothesis makes it possible for us to contribute to the development of the theory. Because of this, we are in a position to definitively determine what is true and what is untrue .

How will Hypothesis help in the Scientific Method?

  • The scientific method begins with observation and inquiry about the natural world when formulating research questions. Researchers can refine their observations and queries into specific, testable research questions with the aid of hypothesis. They provide an investigation with a focused starting point.
  • Hypothesis generate specific predictions regarding the expected outcomes of experiments or observations. These forecasts are founded on the researcher’s current knowledge of the subject. They elucidate what researchers anticipate observing if the hypothesis is true.
  • Hypothesis direct the design of experiments and data collection techniques. Researchers can use them to determine which variables to measure or manipulate, which data to obtain, and how to conduct systematic and controlled research.
  • Following the formulation of a hypothesis and the design of an experiment, researchers collect data through observation, measurement, or experimentation. The collected data is used to verify the hypothesis’s predictions.
  • Hypothesis establish the criteria for evaluating experiment results. The observed data are compared to the predictions generated by the hypothesis. This analysis helps determine whether empirical evidence supports or refutes the hypothesis.
  • The results of experiments or observations are used to derive conclusions regarding the hypothesis. If the data support the predictions, then the hypothesis is supported. If this is not the case, the hypothesis may be revised or rejected, leading to the formulation of new queries and hypothesis.
  • The scientific approach is iterative, resulting in new hypothesis and research issues from previous trials. This cycle of hypothesis generation, testing, and refining drives scientific progress.

Hypothesis

Importance Of Hypothesis

  • Hypothesis are testable statements that enable scientists to determine if their predictions are accurate. This assessment is essential to the scientific method, which is based on empirical evidence.
  • Hypothesis serve as the foundation for designing experiments or data collection techniques. They can be used by researchers to develop protocols and procedures that will produce meaningful results.
  • Hypothesis hold scientists accountable for their assertions. They establish expectations for what the research should reveal and enable others to assess the validity of the findings.
  • Hypothesis aid in identifying the most important variables of a study. The variables can then be measured, manipulated, or analyzed to determine their relationships.
  • Hypothesis assist researchers in allocating their resources efficiently. They ensure that time, money, and effort are spent investigating specific concerns, as opposed to exploring random concepts.
  • Testing hypothesis contribute to the scientific body of knowledge. Whether or not a hypothesis is supported, the results contribute to our understanding of a phenomenon.
  • Hypothesis can result in the creation of theories. When supported by substantive evidence, hypothesis can serve as the foundation for larger theoretical frameworks that explain complex phenomena.
  • Beyond scientific research, hypothesis play a role in the solution of problems in a variety of domains. They enable professionals to make educated assumptions about the causes of problems and to devise solutions.

Research Hypotheses: Did you know that a hypothesis refers to an educated guess or prediction about the outcome of a research study?

It’s like a roadmap guiding researchers towards their destination of knowledge. Just like a compass points north, a well-crafted hypothesis points the way to valuable discoveries in the world of science and inquiry.

Choose the best answer. 

Send Your Results (Optional)

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Further Reading

  • RNA-DNA World Hypothesis
  • BYJU’S. (2023). Hypothesis. Retrieved 01 Septermber 2023, from https://byjus.com/physics/hypothesis/#sources-of-hypothesis
  • Collegedunia. (2023). Hypothesis. Retrieved 1 September 2023, from https://collegedunia.com/exams/hypothesis-science-articleid-7026#d
  • Hussain, D. J. (2022). Hypothesis. Retrieved 01 September 2023, from https://mmhapu.ac.in/doc/eContent/Management/JamesHusain/Research%20Hypothesis%20-Meaning,%20Nature%20&%20Importance-Characteristics%20of%20Good%20%20Hypothesis%20Sem2.pdf
  • Media, D. (2023). Hypothesis in the Scientific Method. Retrieved 01 September 2023, from https://www.verywellmind.com/what-is-a-hypothesis-2795239#toc-hypotheses-examples
  • Rashid, M. H. A. (Apr 20, 2022). Research Methodology. Retrieved 01 September 2023, from https://limbd.org/hypothesis-definitions-functions-characteristics-types-errors-the-process-of-testing-a-hypothesis-hypotheses-in-qualitative-research/#:~:text=Functions%20of%20a%20Hypothesis%3A&text=Specifically%2C%20a%20hypothesis%20serves%20the,providing%20focus%20to%20the%20study.

©BiologyOnline.com. Content provided and moderated by Biology Online Editors.

Last updated on September 8th, 2023

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Hypothesis Examples

Hypothesis Examples

A hypothesis is a prediction of the outcome of a test. It forms the basis for designing an experiment in the scientific method . A good hypothesis is testable, meaning it makes a prediction you can check with observation or experimentation. Here are different hypothesis examples.

Null Hypothesis Examples

The null hypothesis (H 0 ) is also known as the zero-difference or no-difference hypothesis. It predicts that changing one variable ( independent variable ) will have no effect on the variable being measured ( dependent variable ). Here are null hypothesis examples:

  • Plant growth is unaffected by temperature.
  • If you increase temperature, then solubility of salt will increase.
  • Incidence of skin cancer is unrelated to ultraviolet light exposure.
  • All brands of light bulb last equally long.
  • Cats have no preference for the color of cat food.
  • All daisies have the same number of petals.

Sometimes the null hypothesis shows there is a suspected correlation between two variables. For example, if you think plant growth is affected by temperature, you state the null hypothesis: “Plant growth is not affected by temperature.” Why do you do this, rather than say “If you change temperature, plant growth will be affected”? The answer is because it’s easier applying a statistical test that shows, with a high level of confidence, a null hypothesis is correct or incorrect.

Research Hypothesis Examples

A research hypothesis (H 1 ) is a type of hypothesis used to design an experiment. This type of hypothesis is often written as an if-then statement because it’s easy identifying the independent and dependent variables and seeing how one affects the other. If-then statements explore cause and effect. In other cases, the hypothesis shows a correlation between two variables. Here are some research hypothesis examples:

  • If you leave the lights on, then it takes longer for people to fall asleep.
  • If you refrigerate apples, they last longer before going bad.
  • If you keep the curtains closed, then you need less electricity to heat or cool the house (the electric bill is lower).
  • If you leave a bucket of water uncovered, then it evaporates more quickly.
  • Goldfish lose their color if they are not exposed to light.
  • Workers who take vacations are more productive than those who never take time off.

Is It Okay to Disprove a Hypothesis?

Yes! You may even choose to write your hypothesis in such a way that it can be disproved because it’s easier to prove a statement is wrong than to prove it is right. In other cases, if your prediction is incorrect, that doesn’t mean the science is bad. Revising a hypothesis is common. It demonstrates you learned something you did not know before you conducted the experiment.

Test yourself with a Scientific Method Quiz .

  • Mellenbergh, G.J. (2008). Chapter 8: Research designs: Testing of research hypotheses. In H.J. Adèr & G.J. Mellenbergh (eds.), Advising on Research Methods: A Consultant’s Companion . Huizen, The Netherlands: Johannes van Kessel Publishing.
  • Popper, Karl R. (1959). The Logic of Scientific Discovery . Hutchinson & Co. ISBN 3-1614-8410-X.
  • Schick, Theodore; Vaughn, Lewis (2002). How to think about weird things: critical thinking for a New Age . Boston: McGraw-Hill Higher Education. ISBN 0-7674-2048-9.
  • Tobi, Hilde; Kampen, Jarl K. (2018). “Research design: the methodology for interdisciplinary research framework”. Quality & Quantity . 52 (3): 1209–1225. doi: 10.1007/s11135-017-0513-8

Related Posts

What is a scientific hypothesis?

It's the initial building block in the scientific method.

A girl looks at plants in a test tube for a science experiment. What's her scientific hypothesis?

Hypothesis basics

What makes a hypothesis testable.

  • Types of hypotheses
  • Hypothesis versus theory

Additional resources

Bibliography.

A scientific hypothesis is a tentative, testable explanation for a phenomenon in the natural world. It's the initial building block in the scientific method . Many describe it as an "educated guess" based on prior knowledge and observation. While this is true, a hypothesis is more informed than a guess. While an "educated guess" suggests a random prediction based on a person's expertise, developing a hypothesis requires active observation and background research. 

The basic idea of a hypothesis is that there is no predetermined outcome. For a solution to be termed a scientific hypothesis, it has to be an idea that can be supported or refuted through carefully crafted experimentation or observation. This concept, called falsifiability and testability, was advanced in the mid-20th century by Austrian-British philosopher Karl Popper in his famous book "The Logic of Scientific Discovery" (Routledge, 1959).

A key function of a hypothesis is to derive predictions about the results of future experiments and then perform those experiments to see whether they support the predictions.

A hypothesis is usually written in the form of an if-then statement, which gives a possibility (if) and explains what may happen because of the possibility (then). The statement could also include "may," according to California State University, Bakersfield .

Here are some examples of hypothesis statements:

  • If garlic repels fleas, then a dog that is given garlic every day will not get fleas.
  • If sugar causes cavities, then people who eat a lot of candy may be more prone to cavities.
  • If ultraviolet light can damage the eyes, then maybe this light can cause blindness.

A useful hypothesis should be testable and falsifiable. That means that it should be possible to prove it wrong. A theory that can't be proved wrong is nonscientific, according to Karl Popper's 1963 book " Conjectures and Refutations ."

An example of an untestable statement is, "Dogs are better than cats." That's because the definition of "better" is vague and subjective. However, an untestable statement can be reworded to make it testable. For example, the previous statement could be changed to this: "Owning a dog is associated with higher levels of physical fitness than owning a cat." With this statement, the researcher can take measures of physical fitness from dog and cat owners and compare the two.

Types of scientific hypotheses

Elementary-age students study alternative energy using homemade windmills during public school science class.

In an experiment, researchers generally state their hypotheses in two ways. The null hypothesis predicts that there will be no relationship between the variables tested, or no difference between the experimental groups. The alternative hypothesis predicts the opposite: that there will be a difference between the experimental groups. This is usually the hypothesis scientists are most interested in, according to the University of Miami .

For example, a null hypothesis might state, "There will be no difference in the rate of muscle growth between people who take a protein supplement and people who don't." The alternative hypothesis would state, "There will be a difference in the rate of muscle growth between people who take a protein supplement and people who don't."

If the results of the experiment show a relationship between the variables, then the null hypothesis has been rejected in favor of the alternative hypothesis, according to the book " Research Methods in Psychology " (​​BCcampus, 2015). 

There are other ways to describe an alternative hypothesis. The alternative hypothesis above does not specify a direction of the effect, only that there will be a difference between the two groups. That type of prediction is called a two-tailed hypothesis. If a hypothesis specifies a certain direction — for example, that people who take a protein supplement will gain more muscle than people who don't — it is called a one-tailed hypothesis, according to William M. K. Trochim , a professor of Policy Analysis and Management at Cornell University.

Sometimes, errors take place during an experiment. These errors can happen in one of two ways. A type I error is when the null hypothesis is rejected when it is true. This is also known as a false positive. A type II error occurs when the null hypothesis is not rejected when it is false. This is also known as a false negative, according to the University of California, Berkeley . 

A hypothesis can be rejected or modified, but it can never be proved correct 100% of the time. For example, a scientist can form a hypothesis stating that if a certain type of tomato has a gene for red pigment, that type of tomato will be red. During research, the scientist then finds that each tomato of this type is red. Though the findings confirm the hypothesis, there may be a tomato of that type somewhere in the world that isn't red. Thus, the hypothesis is true, but it may not be true 100% of the time.

Scientific theory vs. scientific hypothesis

The best hypotheses are simple. They deal with a relatively narrow set of phenomena. But theories are broader; they generally combine multiple hypotheses into a general explanation for a wide range of phenomena, according to the University of California, Berkeley . For example, a hypothesis might state, "If animals adapt to suit their environments, then birds that live on islands with lots of seeds to eat will have differently shaped beaks than birds that live on islands with lots of insects to eat." After testing many hypotheses like these, Charles Darwin formulated an overarching theory: the theory of evolution by natural selection.

"Theories are the ways that we make sense of what we observe in the natural world," Tanner said. "Theories are structures of ideas that explain and interpret facts." 

  • Read more about writing a hypothesis, from the American Medical Writers Association.
  • Find out why a hypothesis isn't always necessary in science, from The American Biology Teacher.
  • Learn about null and alternative hypotheses, from Prof. Essa on YouTube .

Encyclopedia Britannica. Scientific Hypothesis. Jan. 13, 2022. https://www.britannica.com/science/scientific-hypothesis

Karl Popper, "The Logic of Scientific Discovery," Routledge, 1959.

California State University, Bakersfield, "Formatting a testable hypothesis." https://www.csub.edu/~ddodenhoff/Bio100/Bio100sp04/formattingahypothesis.htm  

Karl Popper, "Conjectures and Refutations," Routledge, 1963.

Price, P., Jhangiani, R., & Chiang, I., "Research Methods of Psychology — 2nd Canadian Edition," BCcampus, 2015.‌

University of Miami, "The Scientific Method" http://www.bio.miami.edu/dana/161/evolution/161app1_scimethod.pdf  

William M.K. Trochim, "Research Methods Knowledge Base," https://conjointly.com/kb/hypotheses-explained/  

University of California, Berkeley, "Multiple Hypothesis Testing and False Discovery Rate" https://www.stat.berkeley.edu/~hhuang/STAT141/Lecture-FDR.pdf  

University of California, Berkeley, "Science at multiple levels" https://undsci.berkeley.edu/article/0_0_0/howscienceworks_19

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hypothesis definition biology examples

Module 1: Introduction to Biology

Experiments and hypotheses, learning outcomes.

  • Form a hypothesis and use it to design a scientific experiment

Now we’ll focus on the methods of scientific inquiry. Science often involves making observations and developing hypotheses. Experiments and further observations are often used to test the hypotheses.

A scientific experiment is a carefully organized procedure in which the scientist intervenes in a system to change something, then observes the result of the change. Scientific inquiry often involves doing experiments, though not always. For example, a scientist studying the mating behaviors of ladybugs might begin with detailed observations of ladybugs mating in their natural habitats. While this research may not be experimental, it is scientific: it involves careful and verifiable observation of the natural world. The same scientist might then treat some of the ladybugs with a hormone hypothesized to trigger mating and observe whether these ladybugs mated sooner or more often than untreated ones. This would qualify as an experiment because the scientist is now making a change in the system and observing the effects.

Forming a Hypothesis

When conducting scientific experiments, researchers develop hypotheses to guide experimental design. A hypothesis is a suggested explanation that is both testable and falsifiable. You must be able to test your hypothesis through observations and research, and it must be possible to prove your hypothesis false.

For example, Michael observes that maple trees lose their leaves in the fall. He might then propose a possible explanation for this observation: “cold weather causes maple trees to lose their leaves in the fall.” This statement is testable. He could grow maple trees in a warm enclosed environment such as a greenhouse and see if their leaves still dropped in the fall. The hypothesis is also falsifiable. If the leaves still dropped in the warm environment, then clearly temperature was not the main factor in causing maple leaves to drop in autumn.

In the Try It below, you can practice recognizing scientific hypotheses. As you consider each statement, try to think as a scientist would: can I test this hypothesis with observations or experiments? Is the statement falsifiable? If the answer to either of these questions is “no,” the statement is not a valid scientific hypothesis.

Practice Questions

Determine whether each following statement is a scientific hypothesis.

Air pollution from automobile exhaust can trigger symptoms in people with asthma.

  • No. This statement is not testable or falsifiable.
  • No. This statement is not testable.
  • No. This statement is not falsifiable.
  • Yes. This statement is testable and falsifiable.

Natural disasters, such as tornadoes, are punishments for bad thoughts and behaviors.

a: No. This statement is not testable or falsifiable. “Bad thoughts and behaviors” are excessively vague and subjective variables that would be impossible to measure or agree upon in a reliable way. The statement might be “falsifiable” if you came up with a counterexample: a “wicked” place that was not punished by a natural disaster. But some would question whether the people in that place were really wicked, and others would continue to predict that a natural disaster was bound to strike that place at some point. There is no reason to suspect that people’s immoral behavior affects the weather unless you bring up the intervention of a supernatural being, making this idea even harder to test.

Testing a Vaccine

Let’s examine the scientific process by discussing an actual scientific experiment conducted by researchers at the University of Washington. These researchers investigated whether a vaccine may reduce the incidence of the human papillomavirus (HPV). The experimental process and results were published in an article titled, “ A controlled trial of a human papillomavirus type 16 vaccine .”

Preliminary observations made by the researchers who conducted the HPV experiment are listed below:

  • Human papillomavirus (HPV) is the most common sexually transmitted virus in the United States.
  • There are about 40 different types of HPV. A significant number of people that have HPV are unaware of it because many of these viruses cause no symptoms.
  • Some types of HPV can cause cervical cancer.
  • About 4,000 women a year die of cervical cancer in the United States.

Practice Question

Researchers have developed a potential vaccine against HPV and want to test it. What is the first testable hypothesis that the researchers should study?

  • HPV causes cervical cancer.
  • People should not have unprotected sex with many partners.
  • People who get the vaccine will not get HPV.
  • The HPV vaccine will protect people against cancer.

Experimental Design

You’ve successfully identified a hypothesis for the University of Washington’s study on HPV: People who get the HPV vaccine will not get HPV.

The next step is to design an experiment that will test this hypothesis. There are several important factors to consider when designing a scientific experiment. First, scientific experiments must have an experimental group. This is the group that receives the experimental treatment necessary to address the hypothesis.

The experimental group receives the vaccine, but how can we know if the vaccine made a difference? Many things may change HPV infection rates in a group of people over time. To clearly show that the vaccine was effective in helping the experimental group, we need to include in our study an otherwise similar control group that does not get the treatment. We can then compare the two groups and determine if the vaccine made a difference. The control group shows us what happens in the absence of the factor under study.

However, the control group cannot get “nothing.” Instead, the control group often receives a placebo. A placebo is a procedure that has no expected therapeutic effect—such as giving a person a sugar pill or a shot containing only plain saline solution with no drug. Scientific studies have shown that the “placebo effect” can alter experimental results because when individuals are told that they are or are not being treated, this knowledge can alter their actions or their emotions, which can then alter the results of the experiment.

Moreover, if the doctor knows which group a patient is in, this can also influence the results of the experiment. Without saying so directly, the doctor may show—through body language or other subtle cues—their views about whether the patient is likely to get well. These errors can then alter the patient’s experience and change the results of the experiment. Therefore, many clinical studies are “double blind.” In these studies, neither the doctor nor the patient knows which group the patient is in until all experimental results have been collected.

Both placebo treatments and double-blind procedures are designed to prevent bias. Bias is any systematic error that makes a particular experimental outcome more or less likely. Errors can happen in any experiment: people make mistakes in measurement, instruments fail, computer glitches can alter data. But most such errors are random and don’t favor one outcome over another. Patients’ belief in a treatment can make it more likely to appear to “work.” Placebos and double-blind procedures are used to level the playing field so that both groups of study subjects are treated equally and share similar beliefs about their treatment.

The scientists who are researching the effectiveness of the HPV vaccine will test their hypothesis by separating 2,392 young women into two groups: the control group and the experimental group. Answer the following questions about these two groups.

  • This group is given a placebo.
  • This group is deliberately infected with HPV.
  • This group is given nothing.
  • This group is given the HPV vaccine.
  • a: This group is given a placebo. A placebo will be a shot, just like the HPV vaccine, but it will have no active ingredient. It may change peoples’ thinking or behavior to have such a shot given to them, but it will not stimulate the immune systems of the subjects in the same way as predicted for the vaccine itself.
  • d: This group is given the HPV vaccine. The experimental group will receive the HPV vaccine and researchers will then be able to see if it works, when compared to the control group.

Experimental Variables

A variable is a characteristic of a subject (in this case, of a person in the study) that can vary over time or among individuals. Sometimes a variable takes the form of a category, such as male or female; often a variable can be measured precisely, such as body height. Ideally, only one variable is different between the control group and the experimental group in a scientific experiment. Otherwise, the researchers will not be able to determine which variable caused any differences seen in the results. For example, imagine that the people in the control group were, on average, much more sexually active than the people in the experimental group. If, at the end of the experiment, the control group had a higher rate of HPV infection, could you confidently determine why? Maybe the experimental subjects were protected by the vaccine, but maybe they were protected by their low level of sexual contact.

To avoid this situation, experimenters make sure that their subject groups are as similar as possible in all variables except for the variable that is being tested in the experiment. This variable, or factor, will be deliberately changed in the experimental group. The one variable that is different between the two groups is called the independent variable. An independent variable is known or hypothesized to cause some outcome. Imagine an educational researcher investigating the effectiveness of a new teaching strategy in a classroom. The experimental group receives the new teaching strategy, while the control group receives the traditional strategy. It is the teaching strategy that is the independent variable in this scenario. In an experiment, the independent variable is the variable that the scientist deliberately changes or imposes on the subjects.

Dependent variables are known or hypothesized consequences; they are the effects that result from changes or differences in an independent variable. In an experiment, the dependent variables are those that the scientist measures before, during, and particularly at the end of the experiment to see if they have changed as expected. The dependent variable must be stated so that it is clear how it will be observed or measured. Rather than comparing “learning” among students (which is a vague and difficult to measure concept), an educational researcher might choose to compare test scores, which are very specific and easy to measure.

In any real-world example, many, many variables MIGHT affect the outcome of an experiment, yet only one or a few independent variables can be tested. Other variables must be kept as similar as possible between the study groups and are called control variables . For our educational research example, if the control group consisted only of people between the ages of 18 and 20 and the experimental group contained people between the ages of 30 and 35, we would not know if it was the teaching strategy or the students’ ages that played a larger role in the results. To avoid this problem, a good study will be set up so that each group contains students with a similar age profile. In a well-designed educational research study, student age will be a controlled variable, along with other possibly important factors like gender, past educational achievement, and pre-existing knowledge of the subject area.

What is the independent variable in this experiment?

  • Sex (all of the subjects will be female)
  • Presence or absence of the HPV vaccine
  • Presence or absence of HPV (the virus)

List three control variables other than age.

What is the dependent variable in this experiment?

  • Sex (male or female)
  • Rates of HPV infection
  • Age (years)
  • Revision and adaptation. Authored by : Shelli Carter and Lumen Learning. Provided by : Lumen Learning. License : CC BY-NC-SA: Attribution-NonCommercial-ShareAlike
  • Scientific Inquiry. Provided by : Open Learning Initiative. Located at : https://oli.cmu.edu/jcourse/workbook/activity/page?context=434a5c2680020ca6017c03488572e0f8 . Project : Introduction to Biology (Open + Free). License : CC BY-NC-SA: Attribution-NonCommercial-ShareAlike

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High school biology

Course: high school biology   >   unit 1.

  • Biology overview
  • Preparing to study biology
  • What is life?
  • The scientific method
  • Data to justify experimental claims examples
  • Scientific method and data analysis
  • Introduction to experimental design
  • Controlled experiments

Biology and the scientific method review

  • Experimental design and bias

The nature of biology

Properties of life.

  • Organization: Living things are highly organized (meaning they contain specialized, coordinated parts) and are made up of one or more cells .
  • Metabolism: Living things must use energy and consume nutrients to carry out the chemical reactions that sustain life. The sum total of the biochemical reactions occurring in an organism is called its metabolism .
  • Homeostasis : Living organisms regulate their internal environment to maintain the relatively narrow range of conditions needed for cell function.
  • Growth : Living organisms undergo regulated growth. Individual cells become larger in size, and multicellular organisms accumulate many cells through cell division.
  • Reproduction : Living organisms can reproduce themselves to create new organisms.
  • Response : Living organisms respond to stimuli or changes in their environment.
  • Evolution : Populations of living organisms can undergo evolution , meaning that the genetic makeup of a population may change over time.

Scientific methodology

Scientific method example: failure to toast.

  • Observation: the toaster won't toast.
  • Question: Why won't my toaster toast?
  • Hypothesis: Maybe the outlet is broken.
  • Prediction: If I plug the toaster into a different outlet, then it will toast the bread.
  • Test of prediction: Plug the toaster into a different outlet and try again.
  • Iteration time!

Experimental design

Reducing errors and bias.

  • Having a large sample size in the experiment: This helps to account for any small differences among the test subjects that may provide unexpected results.
  • Repeating experimental trials multiple times: Errors may result from slight differences in test subjects, or mistakes in methodology or data collection. Repeating trials helps reduce those effects.
  • Including all data points: Sometimes it is tempting to throw away data points that are inconsistent with the proposed hypothesis. However, this makes for an inaccurate study! All data points need to be included, whether they support the hypothesis or not.
  • Using placebos , when appropriate: Placebos prevent the test subjects from knowing whether they received a real therapeutic substance. This helps researchers determine whether a substance has a true effect.
  • Implementing double-blind studies , when appropriate: Double-blind studies prevent researchers from knowing the status of a particular participant. This helps eliminate observer bias.

Communicating findings

Things to remember.

  • A hypothesis is not necessarily the right explanation. Instead, it is a possible explanation that can be tested to see if it is likely correct, or if a new hypothesis needs to be made.
  • Not all explanations can be considered a hypothesis. A hypothesis must be testable and falsifiable in order to be valid. For example, “The universe is beautiful" is not a good hypothesis, because there is no experiment that could test this statement and show it to be false.
  • In most cases, the scientific method is an iterative process. In other words, it's a cycle rather than a straight line. The result of one experiment often becomes feedback that raises questions for more experimentation.
  • Scientists use the word "theory" in a very different way than non-scientists. When many people say "I have a theory," they really mean "I have a guess." Scientific theories, on the other hand, are well-tested and highly reliable scientific explanations of natural phenomena. They unify many repeated observations and data collected from lots of experiments.

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Great Answer

1.1 The Science of Biology

Learning objectives.

By the end of this section, you will be able to do the following:

  • Identify the shared characteristics of the natural sciences
  • Summarize the steps of the scientific method
  • Compare inductive reasoning with deductive reasoning
  • Describe the goals of basic science and applied science

What is biology? In simple terms, biology is the study of life. This is a very broad definition because the scope of biology is vast. Biologists may study anything from the microscopic or submicroscopic view of a cell to ecosystems and the whole living planet ( Figure 1.2 ). Listening to the daily news, you will quickly realize how many aspects of biology we discuss every day. For example, recent news topics include Escherichia coli ( Figure 1.3 ) outbreaks in spinach and Salmonella contamination in peanut butter. Other subjects include efforts toward finding a cure for AIDS, Alzheimer’s disease, and cancer. On a global scale, many researchers are committed to finding ways to protect the planet, solve environmental issues, and reduce the effects of climate change. All of these diverse endeavors are related to different facets of the discipline of biology.

The Process of Science

Biology is a science, but what exactly is science? What does the study of biology share with other scientific disciplines? We can define science (from the Latin scientia , meaning “knowledge”) as knowledge that covers general truths or the operation of general laws, especially when acquired and tested by the scientific method. It becomes clear from this definition that applying scientific method plays a major role in science. The scientific method is a method of research with defined steps that include experiments and careful observation.

We will examine scientific method steps in detail later, but one of the most important aspects of this method is the testing of hypotheses by means of repeatable experiments. A hypothesis is a suggested explanation for an event, which one can test. Although using the scientific method is inherent to science, it is inadequate in determining what science is. This is because it is relatively easy to apply the scientific method to disciplines such as physics and chemistry, but when it comes to disciplines like archaeology, psychology, and geology, the scientific method becomes less applicable as repeating experiments becomes more difficult.

These areas of study are still sciences, however. Consider archaeology—even though one cannot perform repeatable experiments, hypotheses may still be supported. For instance, archaeologists can hypothesize that an ancient culture existed based on finding a piece of pottery. They could make further hypotheses about various characteristics of this culture, which could be correct or false through continued support or contradictions from other findings. A hypothesis may become a verified theory. A theory is a tested and confirmed explanation for observations or phenomena. Therefore, we may be better off to define science as fields of study that attempt to comprehend the nature of the universe.

Natural Sciences

What would you expect to see in a museum of natural sciences? Frogs? Plants? Dinosaur skeletons? Exhibits about how the brain functions? A planetarium? Gems and minerals? Maybe all of the above? Science includes such diverse fields as astronomy, biology, computer sciences, geology, logic, physics, chemistry, and mathematics ( Figure 1.4 ). However, scientists consider those fields of science related to the physical world and its phenomena and processes natural sciences . Thus, a museum of natural sciences might contain any of the items listed above.

There is no complete agreement when it comes to defining what the natural sciences include, however. For some experts, the natural sciences are astronomy, biology, chemistry, earth science, and physics. Other scholars choose to divide natural sciences into life sciences , which study living things and include biology, and physical sciences , which study nonliving matter and include astronomy, geology, physics, and chemistry. Some disciplines such as biophysics and biochemistry build on both life and physical sciences and are interdisciplinary. Some refer to natural sciences as “hard science” because they rely on the use of quantitative data. Social sciences that study society and human behavior are more likely to use qualitative assessments to drive investigations and findings.

Not surprisingly, the natural science of biology has many branches or subdisciplines. Cell biologists study cell structure and function, while biologists who study anatomy investigate the structure of an entire organism. Those biologists studying physiology, however, focus on the internal functioning of an organism. Some areas of biology focus on only particular types of living things. For example, botanists explore plants, while zoologists specialize in animals.

Scientific Reasoning

One thing is common to all forms of science: an ultimate goal “to know.” Curiosity and inquiry are the driving forces for the development of science. Scientists seek to understand the world and the way it operates. To do this, they use two methods of logical thinking: inductive reasoning and deductive reasoning.

Inductive reasoning is a form of logical thinking that uses related observations to arrive at a general conclusion. This type of reasoning is common in descriptive science. A life scientist such as a biologist makes observations and records them. These data can be qualitative or quantitative, and one can supplement the raw data with drawings, pictures, photos, or videos. From many observations, the scientist can infer conclusions (inductions) based on evidence. Inductive reasoning involves formulating generalizations inferred from careful observation and analyzing a large amount of data. Brain studies provide an example. In this type of research, scientists observe many live brains while people are engaged in a specific activity, such as viewing images of food. The scientist then predicts the part of the brain that “lights up” during this activity to be the part controlling the response to the selected stimulus, in this case, images of food. Excess absorption of radioactive sugar derivatives by active areas of the brain causes the various areas to "light up". Scientists use a scanner to observe the resultant increase in radioactivity. Then, researchers can stimulate that part of the brain to see if similar responses result.

Deductive reasoning or deduction is the type of logic used in hypothesis-based science. In deductive reasoning, the pattern of thinking moves in the opposite direction as compared to inductive reasoning. Deductive reasoning is a form of logical thinking that uses a general principle or law to predict specific results. From those general principles, a scientist can deduce and predict the specific results that would be valid as long as the general principles are valid. Studies in climate change can illustrate this type of reasoning. For example, scientists may predict that if the climate becomes warmer in a particular region, then the distribution of plants and animals should change.

Both types of logical thinking are related to the two main pathways of scientific study: descriptive science and hypothesis-based science. Descriptive (or discovery) science , which is usually inductive, aims to observe, explore, and discover, while hypothesis-based science , which is usually deductive, begins with a specific question or problem and a potential answer or solution that one can test. The boundary between these two forms of study is often blurred, and most scientific endeavors combine both approaches. The fuzzy boundary becomes apparent when thinking about how easily observation can lead to specific questions. For example, a gentleman in the 1940s observed that the burr seeds that stuck to his clothes and his dog’s fur had a tiny hook structure. On closer inspection, he discovered that the burrs’ gripping device was more reliable than a zipper. He eventually experimented to find the best material that acted similarly, and produced the hook-and-loop fastener popularly known today as Velcro. Descriptive science and hypothesis-based science are in continuous dialogue.

The Scientific Method

Biologists study the living world by posing questions about it and seeking science-based responses. Known as scientific method, this approach is common to other sciences as well. The scientific method was used even in ancient times, but England’s Sir Francis Bacon (1561–1626) first documented it ( Figure 1.5 ). He set up inductive methods for scientific inquiry. The scientific method is not used only by biologists; researchers from almost all fields of study can apply it as a logical, rational problem-solving method.

The scientific process typically starts with an observation (often a problem to solve) that leads to a question. Let’s think about a simple problem that starts with an observation and apply the scientific method to solve the problem. One Monday morning, a student arrives at class and quickly discovers that the classroom is too warm. That is an observation that also describes a problem: the classroom is too warm. The student then asks a question: “Why is the classroom so warm?”

Proposing a Hypothesis

Recall that a hypothesis is a suggested explanation that one can test. To solve a problem, one can propose several hypotheses. For example, one hypothesis might be, “The classroom is warm because no one turned on the air conditioning.” However, there could be other responses to the question, and therefore one may propose other hypotheses. A second hypothesis might be, “The classroom is warm because there is a power failure, and so the air conditioning doesn’t work.”

Once one has selected a hypothesis, the student can make a prediction. A prediction is similar to a hypothesis but it typically has the format “If . . . then . . . .” For example, the prediction for the first hypothesis might be, “ If the student turns on the air conditioning, then the classroom will no longer be too warm.”

Testing a Hypothesis

A valid hypothesis must be testable. It should also be falsifiable , meaning that experimental results can disprove it. Importantly, science does not claim to “prove” anything because scientific understandings are always subject to modification with further information. This step—openness to disproving ideas—is what distinguishes sciences from non-sciences. The presence of the supernatural, for instance, is neither testable nor falsifiable. To test a hypothesis, a researcher will conduct one or more experiments designed to eliminate one or more of the hypotheses. Each experiment will have one or more variables and one or more controls. A variable is any part of the experiment that can vary or change during the experiment. The control group contains every feature of the experimental group except it is not given the manipulation that the researcher hypothesizes. Therefore, if the experimental group's results differ from the control group, the difference must be due to the hypothesized manipulation, rather than some outside factor. Look for the variables and controls in the examples that follow. To test the first hypothesis, the student would find out if the air conditioning is on. If the air conditioning is turned on but does not work, there should be another reason, and the student should reject this hypothesis. To test the second hypothesis, the student could check if the lights in the classroom are functional. If so, there is no power failure and the student should reject this hypothesis. The students should test each hypothesis by carrying out appropriate experiments. Be aware that rejecting one hypothesis does not determine whether or not one can accept the other hypotheses. It simply eliminates one hypothesis that is not valid ( Figure 1.6 ). Using the scientific method, the student rejects the hypotheses that are inconsistent with experimental data.

While this “warm classroom” example is based on observational results, other hypotheses and experiments might have clearer controls. For instance, a student might attend class on Monday and realize she had difficulty concentrating on the lecture. One observation to explain this occurrence might be, “When I eat breakfast before class, I am better able to pay attention.” The student could then design an experiment with a control to test this hypothesis.

In hypothesis-based science, researchers predict specific results from a general premise. We call this type of reasoning deductive reasoning: deduction proceeds from the general to the particular. However, the reverse of the process is also possible: sometimes, scientists reach a general conclusion from a number of specific observations. We call this type of reasoning inductive reasoning, and it proceeds from the particular to the general. Researchers often use inductive and deductive reasoning in tandem to advance scientific knowledge ( Figure 1.7 ). In recent years a new approach of testing hypotheses has developed as a result of an exponential growth of data deposited in various databases. Using computer algorithms and statistical analyses of data in databases, a new field of so-called "data research" (also referred to as "in silico" research) provides new methods of data analyses and their interpretation. This will increase the demand for specialists in both biology and computer science, a promising career opportunity.

Visual Connection

In the example below, the scientific method is used to solve an everyday problem. Match the scientific method steps (numbered items) with the process of solving the everyday problem (lettered items). Based on the results of the experiment, is the hypothesis correct? If it is incorrect, propose some alternative hypotheses.

Decide if each of the following is an example of inductive or deductive reasoning.

  • All flying birds and insects have wings. Birds and insects flap their wings as they move through the air. Therefore, wings enable flight.
  • Insects generally survive mild winters better than harsh ones. Therefore, insect pests will become more problematic if global temperatures increase.
  • Chromosomes, the carriers of DNA, are distributed evenly between the daughter cells during cell division. Therefore, each daughter cell will have the same chromosome set as the mother cell.
  • Animals as diverse as humans, insects, and wolves all exhibit social behavior. Therefore, social behavior must have an evolutionary advantage.

The scientific method may seem too rigid and structured. It is important to keep in mind that, although scientists often follow this sequence, there is flexibility. Sometimes an experiment leads to conclusions that favor a change in approach. Often, an experiment brings entirely new scientific questions to the puzzle. Many times, science does not operate in a linear fashion. Instead, scientists continually draw inferences and make generalizations, finding patterns as their research proceeds. Scientific reasoning is more complex than the scientific method alone suggests. Notice, too, that we can apply the scientific method to solving problems that aren’t necessarily scientific in nature.

Two Types of Science: Basic Science and Applied Science

The scientific community has been debating for the last few decades about the value of different types of science. Is it valuable to pursue science for the sake of simply gaining knowledge, or does scientific knowledge only have worth if we can apply it to solving a specific problem or to bettering our lives? This question focuses on the differences between two types of science: basic science and applied science.

Basic science or “pure” science seeks to expand knowledge regardless of the short-term application of that knowledge. It is not focused on developing a product or a service of immediate public or commercial value. The immediate goal of basic science is knowledge for knowledge’s sake, although this does not mean that, in the end, it may not result in a practical application.

In contrast, applied science or “technology,” aims to use science to solve real-world problems, making it possible, for example, to improve a crop yield, find a cure for a particular disease, or save animals threatened by a natural disaster ( Figure 1.8 ). In applied science, the problem is usually defined for the researcher.

Some individuals may perceive applied science as “useful” and basic science as “useless.” A question these people might pose to a scientist advocating knowledge acquisition would be, “What for?” However, a careful look at the history of science reveals that basic knowledge has resulted in many remarkable applications of great value. Many scientists think that a basic understanding of science is necessary before researchers develop an application, therefore, applied science relies on the results that researchers generate through basic science. Other scientists think that it is time to move on from basic science in order to find solutions to actual problems. Both approaches are valid. It is true that there are problems that demand immediate attention; however, scientists would find few solutions without the help of the wide knowledge foundation that basic science generates.

One example of how basic and applied science can work together to solve practical problems occurred after the discovery of DNA structure led to an understanding of the molecular mechanisms governing DNA replication. DNA strands, unique in every human, are in our cells, where they provide the instructions necessary for life. When DNA replicates, it produces new copies of itself, shortly before a cell divides. Understanding DNA replication mechanisms enabled scientists to develop laboratory techniques that researchers now use to identify genetic diseases, pinpoint individuals who were at a crime scene, and determine paternity. Without basic science, it is unlikely that applied science could exist.

Another example of the link between basic and applied research is the Human Genome Project, a study in which researchers analyzed and mapped each human chromosome to determine the precise sequence of DNA subunits and each gene's exact location. (The gene is the basic unit of heredity represented by a specific DNA segment that codes for a functional molecule. An individual’s complete collection of genes is their genome.) Researchers have studied other less complex organisms as part of this project in order to gain a better understanding of human chromosomes. The Human Genome Project ( Figure 1.9 ) relied on basic research with simple organisms and, later, with the human genome. An important end goal eventually became using the data for applied research, seeking cures and early diagnoses for genetically related diseases.

While scientists usually carefully plan research efforts in both basic science and applied science, note that some discoveries are made by serendipity , that is, by means of a fortunate accident or a lucky surprise. Scottish biologist Alexander Fleming discovered penicillin when he accidentally left a petri dish of Staphylococcus bacteria open. An unwanted mold grew on the dish, killing the bacteria. Fleming's curiosity to investigate the reason behind the bacterial death, followed by his experiments, led to the discovery of the antibiotic penicillin, which is produced by the fungus Penicillium . Even in the highly organized world of science, luck—when combined with an observant, curious mind—can lead to unexpected breakthroughs.

Reporting Scientific Work

Whether scientific research is basic science or applied science, scientists must share their findings in order for other researchers to expand and build upon their discoveries. Collaboration with other scientists—when planning, conducting, and analyzing results—is important for scientific research. For this reason, important aspects of a scientist’s work are communicating with peers and disseminating results to peers. Scientists can share results by presenting them at a scientific meeting or conference, but this approach can reach only the select few who are present. Instead, most scientists present their results in peer-reviewed manuscripts that are published in scientific journals. Peer-reviewed manuscripts are scientific papers that a scientist’s colleagues or peers review. These colleagues are qualified individuals, often experts in the same research area, who judge whether or not the scientist’s work is suitable for publication. The process of peer review helps to ensure that the research in a scientific paper or grant proposal is original, significant, logical, and thorough. Grant proposals, which are requests for research funding, are also subject to peer review. Scientists publish their work so other scientists can reproduce their experiments under similar or different conditions to expand on the findings.

A scientific paper is very different from creative writing. Although creativity is required to design experiments, there are fixed guidelines when it comes to presenting scientific results. First, scientific writing must be brief, concise, and accurate. A scientific paper needs to be succinct but detailed enough to allow peers to reproduce the experiments.

The scientific paper consists of several specific sections—introduction, materials and methods, results, and discussion. This structure is sometimes called the “IMRaD” format. There are usually acknowledgment and reference sections as well as an abstract (a concise summary) at the beginning of the paper. There might be additional sections depending on the type of paper and the journal where it will be published. For example, some review papers require an outline.

The introduction starts with brief, but broad, background information about what is known in the field. A good introduction also gives the rationale of the work. It justifies the work carried out and also briefly mentions the end of the paper, where the researcher will present the hypothesis or research question driving the research. The introduction refers to the published scientific work of others and therefore requires citations following the style of the journal. Using the work or ideas of others without proper citation is plagiarism .

The materials and methods section includes a complete and accurate description of the substances the researchers use, and the method and techniques they use to gather data. The description should be thorough enough to allow another researcher to repeat the experiment and obtain similar results, but it does not have to be verbose. This section will also include information on how the researchers made measurements and the types of calculations and statistical analyses they used to examine raw data. Although the materials and methods section gives an accurate description of the experiments, it does not discuss them.

Some journals require a results section followed by a discussion section, but it is more common to combine both. If the journal does not allow combining both sections, the results section simply narrates the findings without any further interpretation. The researchers present results with tables or graphs, but they do not present duplicate information. In the discussion section, the researchers will interpret the results, describe how variables may be related, and attempt to explain the observations. It is indispensable to conduct an extensive literature search to put the results in the context of previously published scientific research. Therefore, researchers include proper citations in this section as well.

Finally, the conclusion section summarizes the importance of the experimental findings. While the scientific paper almost certainly answers one or more scientific questions that the researchers stated, any good research should lead to more questions. Therefore, a well-done scientific paper allows the researchers and others to continue and expand on the findings.

Review articles do not follow the IMRAD format because they do not present original scientific findings, or primary literature. Instead, they summarize and comment on findings that were published as primary literature and typically include extensive reference sections.

Scientific Ethics

Scientists must ensure that their efforts do not cause undue damage to humans, animals, or the environment. They also must ensure that their research and communications are free of bias and that they properly balance financial, legal, safety, replicability, and other considerations. All scientists -- and many people in other fields -- have these ethical obligations, but those in the life sciences have a particular obligation because their research may involve people or other living things. Bioethics is thus an important and continually evolving field, in which researchers collaborate with other thinkers and organizations. They work to define guidelines for current practice, and also continually consider new developments and emerging technologies in order to form answers for the years and decades to come.

For example, bioethicists may examine the implications of gene editing technologies, including the ability to create organisms that may displace others in the environment, as well as the ability to “design” human beings. In that effort, ethicists will likely seek to balance the positive outcomes -- such as improved therapies or prevention of certain illnesses -- with negative outcomes.

Unfortunately, the emergence of bioethics as a field came after a number of clearly unethical practices, where biologists did not treat research subjects with dignity and in some cases did them harm. In the 1932 Tuskegee syphilis study, 399 African American men were diagnosed with syphilis but were never informed that they had the disease, leaving them to live with and pass on the illness to others. Doctors even withheld proven medications because the goal of the study was to understand the impact of untreated syphilis on Black men.

While the decisions made in the Tuskegee study are unjustifiable, some decisions are genuinely difficult to make. Bioethicists work to establish moral and dignifying approaches before such decisions come to pass. For example, doctors rely on artificial intelligence and robotics for medical diagnosis and treatment; in the near future, even more responsibility will lie with machines. Who will be responsible for medical decisions? Who will explain to families if a procedure doesn’t go as planned? And, since such treatments will likely be expensive, who will decide who has access to them and who does not? These are all questions bioethicists seek to answer, and are the types of considerations that all scientific researchers take into account when designing and conducting studies.

Bioethics are not simple, and often leave scientists balancing benefits with harm. In this text and course, you will discuss medical discoveries, vaccines, and research that, at their core, have an ethical complexity or, in the view of many, an ethical lapse. In 1951, Henrietta Lacks , a 30-year-old African American woman, was diagnosed with cervical cancer at Johns Hopkins Hospital. Unique characteristics of her illnesses gave her cells the ability to divide continuously, essentially making them “immortal.” Without her knowledge or permission, researchers took samples of her cells and with them created the immortal HeLa cell line. These cells have contributed to major medical discoveries, including the polio vaccine. Many researchers mentioned in subsequent sections of the text relied on HeLa cell research as at least a component of their work related to cancer, AIDS, cell aging, and even very recently in COVID-19 research.

Today, harvesting tissue or organs from a dying patient without consent is not only considered unethical but illegal, regardless of whether such an act could save other patients’ lives. Is it ethical, then, for scientists to continue to use Lacks’s tissues for research, even though they were obtained illegally by today’s standards? Should Lacks be mentioned as a contributor to the research based on her cells, and should she be cited in the several Nobel Prizes that have been awarded through such work? Finally, should medical companies be obligated to pay Lacks’ family (which had financial difficulties) a portion of the billions of dollars in revenue earned through medicines that benefited from HeLa cell research? How would Henrietta Lacks feel about this? Because she was never asked, we will never know.

To avoid such situations, the role of ethics in scientific research is to ask such questions before, during, and after research or practice takes place, as well as to adhere to established professional principles and consider the dignity and safety of all organisms involved or affected by the work.

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Scientific Hypothesis Examples

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A hypothesis is an educated guess about what you think will happen in a scientific experiment, based on your observations. Before conducting the experiment, you propose a hypothesis so that you can determine if your prediction is supported.

There are several ways you can state a hypothesis, but the best hypotheses are ones you can test and easily refute. Why would you want to disprove or discard your own hypothesis? Well, it is the easiest way to demonstrate that two factors are related. Here are some good scientific hypothesis examples:

  • Hypothesis: All forks have three tines. This would be disproven if you find any fork with a different number of tines.
  • Hypothesis: There is no relationship between smoking and lung cancer. While it is difficult to establish cause and effect in health issues, you can apply statistics to data to discredit or support this hypothesis.
  • Hypothesis: Plants require liquid water to survive. This would be disproven if you find a plant that doesn't need it.
  • Hypothesis: Cats do not show a paw preference (equivalent to being right- or left-handed). You could gather data around the number of times cats bat at a toy with either paw and analyze the data to determine whether cats, on the whole, favor one paw over the other. Be careful here, because individual cats, like people, might (or might not) express a preference. A large sample size would be helpful.
  • Hypothesis: If plants are watered with a 10% detergent solution, their growth will be negatively affected. Some people prefer to state a hypothesis in an "If, then" format. An alternate hypothesis might be: Plant growth will be unaffected by water with a 10% detergent solution.
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How to Write a Great Hypothesis

Hypothesis Definition, Format, Examples, and Tips

Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

hypothesis definition biology examples

Amy Morin, LCSW, is a psychotherapist and international bestselling author. Her books, including "13 Things Mentally Strong People Don't Do," have been translated into more than 40 languages. Her TEDx talk,  "The Secret of Becoming Mentally Strong," is one of the most viewed talks of all time.

hypothesis definition biology examples

Verywell / Alex Dos Diaz

  • The Scientific Method

Hypothesis Format

Falsifiability of a hypothesis.

  • Operationalization

Hypothesis Types

Hypotheses examples.

  • Collecting Data

A hypothesis is a tentative statement about the relationship between two or more variables. It is a specific, testable prediction about what you expect to happen in a study. It is a preliminary answer to your question that helps guide the research process.

Consider a study designed to examine the relationship between sleep deprivation and test performance. The hypothesis might be: "This study is designed to assess the hypothesis that sleep-deprived people will perform worse on a test than individuals who are not sleep-deprived."

At a Glance

A hypothesis is crucial to scientific research because it offers a clear direction for what the researchers are looking to find. This allows them to design experiments to test their predictions and add to our scientific knowledge about the world. This article explores how a hypothesis is used in psychology research, how to write a good hypothesis, and the different types of hypotheses you might use.

The Hypothesis in the Scientific Method

In the scientific method , whether it involves research in psychology, biology, or some other area, a hypothesis represents what the researchers think will happen in an experiment. The scientific method involves the following steps:

  • Forming a question
  • Performing background research
  • Creating a hypothesis
  • Designing an experiment
  • Collecting data
  • Analyzing the results
  • Drawing conclusions
  • Communicating the results

The hypothesis is a prediction, but it involves more than a guess. Most of the time, the hypothesis begins with a question which is then explored through background research. At this point, researchers then begin to develop a testable hypothesis.

Unless you are creating an exploratory study, your hypothesis should always explain what you  expect  to happen.

In a study exploring the effects of a particular drug, the hypothesis might be that researchers expect the drug to have some type of effect on the symptoms of a specific illness. In psychology, the hypothesis might focus on how a certain aspect of the environment might influence a particular behavior.

Remember, a hypothesis does not have to be correct. While the hypothesis predicts what the researchers expect to see, the goal of the research is to determine whether this guess is right or wrong. When conducting an experiment, researchers might explore numerous factors to determine which ones might contribute to the ultimate outcome.

In many cases, researchers may find that the results of an experiment  do not  support the original hypothesis. When writing up these results, the researchers might suggest other options that should be explored in future studies.

In many cases, researchers might draw a hypothesis from a specific theory or build on previous research. For example, prior research has shown that stress can impact the immune system. So a researcher might hypothesize: "People with high-stress levels will be more likely to contract a common cold after being exposed to the virus than people who have low-stress levels."

In other instances, researchers might look at commonly held beliefs or folk wisdom. "Birds of a feather flock together" is one example of folk adage that a psychologist might try to investigate. The researcher might pose a specific hypothesis that "People tend to select romantic partners who are similar to them in interests and educational level."

Elements of a Good Hypothesis

So how do you write a good hypothesis? When trying to come up with a hypothesis for your research or experiments, ask yourself the following questions:

  • Is your hypothesis based on your research on a topic?
  • Can your hypothesis be tested?
  • Does your hypothesis include independent and dependent variables?

Before you come up with a specific hypothesis, spend some time doing background research. Once you have completed a literature review, start thinking about potential questions you still have. Pay attention to the discussion section in the  journal articles you read . Many authors will suggest questions that still need to be explored.

How to Formulate a Good Hypothesis

To form a hypothesis, you should take these steps:

  • Collect as many observations about a topic or problem as you can.
  • Evaluate these observations and look for possible causes of the problem.
  • Create a list of possible explanations that you might want to explore.
  • After you have developed some possible hypotheses, think of ways that you could confirm or disprove each hypothesis through experimentation. This is known as falsifiability.

In the scientific method ,  falsifiability is an important part of any valid hypothesis. In order to test a claim scientifically, it must be possible that the claim could be proven false.

Students sometimes confuse the idea of falsifiability with the idea that it means that something is false, which is not the case. What falsifiability means is that  if  something was false, then it is possible to demonstrate that it is false.

One of the hallmarks of pseudoscience is that it makes claims that cannot be refuted or proven false.

The Importance of Operational Definitions

A variable is a factor or element that can be changed and manipulated in ways that are observable and measurable. However, the researcher must also define how the variable will be manipulated and measured in the study.

Operational definitions are specific definitions for all relevant factors in a study. This process helps make vague or ambiguous concepts detailed and measurable.

For example, a researcher might operationally define the variable " test anxiety " as the results of a self-report measure of anxiety experienced during an exam. A "study habits" variable might be defined by the amount of studying that actually occurs as measured by time.

These precise descriptions are important because many things can be measured in various ways. Clearly defining these variables and how they are measured helps ensure that other researchers can replicate your results.

Replicability

One of the basic principles of any type of scientific research is that the results must be replicable.

Replication means repeating an experiment in the same way to produce the same results. By clearly detailing the specifics of how the variables were measured and manipulated, other researchers can better understand the results and repeat the study if needed.

Some variables are more difficult than others to define. For example, how would you operationally define a variable such as aggression ? For obvious ethical reasons, researchers cannot create a situation in which a person behaves aggressively toward others.

To measure this variable, the researcher must devise a measurement that assesses aggressive behavior without harming others. The researcher might utilize a simulated task to measure aggressiveness in this situation.

Hypothesis Checklist

  • Does your hypothesis focus on something that you can actually test?
  • Does your hypothesis include both an independent and dependent variable?
  • Can you manipulate the variables?
  • Can your hypothesis be tested without violating ethical standards?

The hypothesis you use will depend on what you are investigating and hoping to find. Some of the main types of hypotheses that you might use include:

  • Simple hypothesis : This type of hypothesis suggests there is a relationship between one independent variable and one dependent variable.
  • Complex hypothesis : This type suggests a relationship between three or more variables, such as two independent and dependent variables.
  • Null hypothesis : This hypothesis suggests no relationship exists between two or more variables.
  • Alternative hypothesis : This hypothesis states the opposite of the null hypothesis.
  • Statistical hypothesis : This hypothesis uses statistical analysis to evaluate a representative population sample and then generalizes the findings to the larger group.
  • Logical hypothesis : This hypothesis assumes a relationship between variables without collecting data or evidence.

A hypothesis often follows a basic format of "If {this happens} then {this will happen}." One way to structure your hypothesis is to describe what will happen to the  dependent variable  if you change the  independent variable .

The basic format might be: "If {these changes are made to a certain independent variable}, then we will observe {a change in a specific dependent variable}."

A few examples of simple hypotheses:

  • "Students who eat breakfast will perform better on a math exam than students who do not eat breakfast."
  • "Students who experience test anxiety before an English exam will get lower scores than students who do not experience test anxiety."​
  • "Motorists who talk on the phone while driving will be more likely to make errors on a driving course than those who do not talk on the phone."
  • "Children who receive a new reading intervention will have higher reading scores than students who do not receive the intervention."

Examples of a complex hypothesis include:

  • "People with high-sugar diets and sedentary activity levels are more likely to develop depression."
  • "Younger people who are regularly exposed to green, outdoor areas have better subjective well-being than older adults who have limited exposure to green spaces."

Examples of a null hypothesis include:

  • "There is no difference in anxiety levels between people who take St. John's wort supplements and those who do not."
  • "There is no difference in scores on a memory recall task between children and adults."
  • "There is no difference in aggression levels between children who play first-person shooter games and those who do not."

Examples of an alternative hypothesis:

  • "People who take St. John's wort supplements will have less anxiety than those who do not."
  • "Adults will perform better on a memory task than children."
  • "Children who play first-person shooter games will show higher levels of aggression than children who do not." 

Collecting Data on Your Hypothesis

Once a researcher has formed a testable hypothesis, the next step is to select a research design and start collecting data. The research method depends largely on exactly what they are studying. There are two basic types of research methods: descriptive research and experimental research.

Descriptive Research Methods

Descriptive research such as  case studies ,  naturalistic observations , and surveys are often used when  conducting an experiment is difficult or impossible. These methods are best used to describe different aspects of a behavior or psychological phenomenon.

Once a researcher has collected data using descriptive methods, a  correlational study  can examine how the variables are related. This research method might be used to investigate a hypothesis that is difficult to test experimentally.

Experimental Research Methods

Experimental methods  are used to demonstrate causal relationships between variables. In an experiment, the researcher systematically manipulates a variable of interest (known as the independent variable) and measures the effect on another variable (known as the dependent variable).

Unlike correlational studies, which can only be used to determine if there is a relationship between two variables, experimental methods can be used to determine the actual nature of the relationship—whether changes in one variable actually  cause  another to change.

The hypothesis is a critical part of any scientific exploration. It represents what researchers expect to find in a study or experiment. In situations where the hypothesis is unsupported by the research, the research still has value. Such research helps us better understand how different aspects of the natural world relate to one another. It also helps us develop new hypotheses that can then be tested in the future.

Thompson WH, Skau S. On the scope of scientific hypotheses .  R Soc Open Sci . 2023;10(8):230607. doi:10.1098/rsos.230607

Taran S, Adhikari NKJ, Fan E. Falsifiability in medicine: what clinicians can learn from Karl Popper [published correction appears in Intensive Care Med. 2021 Jun 17;:].  Intensive Care Med . 2021;47(9):1054-1056. doi:10.1007/s00134-021-06432-z

Eyler AA. Research Methods for Public Health . 1st ed. Springer Publishing Company; 2020. doi:10.1891/9780826182067.0004

Nosek BA, Errington TM. What is replication ?  PLoS Biol . 2020;18(3):e3000691. doi:10.1371/journal.pbio.3000691

Aggarwal R, Ranganathan P. Study designs: Part 2 - Descriptive studies .  Perspect Clin Res . 2019;10(1):34-36. doi:10.4103/picr.PICR_154_18

Nevid J. Psychology: Concepts and Applications. Wadworth, 2013.

By Kendra Cherry, MSEd Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

Scientific Hypothesis — Definition & Examples - Expii

Scientific hypothesis — definition & examples, explanations (3).

hypothesis definition biology examples

Scientific Predictions

A comic strip explaining what a hypothesis is, featuring a scientist and his mouse companion. The text reads: A hypothesis is a proposed prediction or explanation based on evidence. It is typically written in "If...then..." format. Because a hypothesis is the starting point for further explanation, it must be able to be tested via the scientific method. Once you have your hypothesis, the next thing is to test it, using—you guessed it—the scientific method. Just remember—it's not important if your hypothesis turns out to be right. The important thing is to learn more about your question!

Image source: By Sylvia Freeman

Related Lessons

How can i write a good hypothesis.

If you are doing a scientific experiment and you want to discover something cool, you need to begin with a strong working hypothesis. This will form the basis and conceptual framework for the rest of your experiment.

It should start with a good research question. You might base this on an observation of something in the natural world. Maybe you think that if a frog is given caffeine, then it could jump further. Your research question might be "Does caffeine influence the jumping activity of frogs?"

The image shows a frog sitting in front of a cup of coffee.

Image source: By Sarah Morgan

Once you have your research question, you can start to form a hypothesis (plural: hypotheses). Most hypotheses follow this structure:

"If woah––––––, then woah––––––will happen."

An example using this structure is "if a frog drinks an ounce of coffee, then the frog will be able to jump at least twice as far".

What Is Changing?

In the hypothesis, you want to include your dependent and independent variables . An independent variable is something that you control. In this case, it would be if you allow the frog to drink coffee or not. A dependent variable is a variable that is changed because of the independent variable . In this case, the distance that the frog jumps is the dependent variable.

Basically, if the independent variable changes, then the dependent variable will change because of it.

Sometimes, you can even follow the structure of "If woah––––––, then woah––––––will happen because woah––––––".

In this structure, you can give more details as to why you believe your idea is true. For example, "If a frog drinks an ounce of coffee, then the frog will be able to jump at least twice as far because the frog has more energy ".

You also want to remember that your hypothesis should be testable . For example, it would be really difficult to test "if red is the best color, then the most popular flavor of drink is peach".

Now that everything is set up, you're ready to begin hypothesis testing through experiments.

What Happens If You Can't Prove Your Hypothesis?

Not every scientific experiment is a success. If you can't prove your original idea, then you've still discovered that the null hypothesis is true. This is basically the opposite of your original prediction (which is also called the alternative hypothesis).

You wanted to prove that "if a frog drinks an ounce of coffee, then the frog will be able to jump at least twice as far". The opposite of that is "if a frog drinks an ounce of coffee, then the frog will not be able to jump at least twice as far", or "the frog will only jump a normal distance".

What Is an Example of a Hypothesis?

Scientists Matthew Meselson and Franklin Stahl hypothesized that DNA is the "transforming principle" in organisms . After finishing their experiments, they were able to confirm their hypothesis based on the data they collected.

Scientists retest and build on the hypotheses of other scientists using new research methods. For example, because of Meselson and Stahl's hypothesizing and experimentation, scientists now know lots about DNA and can do things like make genetically modified organisms .

hypothesis definition biology examples

(Video) The Scientific Method

by Teacher's Pet

hypothesis definition biology examples

The word hypothesis basically means a prediction about what will happen. It is an educated guess and the framework of an experiment. Once you think of one, you can design an experiment to test it. Remember, you cannot prove a hypothesis is true. You can only see if the data supports your claim or not.

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What is a Hypothesis – Types, Examples and Writing Guide

Table of Contents

What is a Hypothesis

Definition:

Hypothesis is an educated guess or proposed explanation for a phenomenon, based on some initial observations or data. It is a tentative statement that can be tested and potentially proven or disproven through further investigation and experimentation.

Hypothesis is often used in scientific research to guide the design of experiments and the collection and analysis of data. It is an essential element of the scientific method, as it allows researchers to make predictions about the outcome of their experiments and to test those predictions to determine their accuracy.

Types of Hypothesis

Types of Hypothesis are as follows:

Research Hypothesis

A research hypothesis is a statement that predicts a relationship between variables. It is usually formulated as a specific statement that can be tested through research, and it is often used in scientific research to guide the design of experiments.

Null Hypothesis

The null hypothesis is a statement that assumes there is no significant difference or relationship between variables. It is often used as a starting point for testing the research hypothesis, and if the results of the study reject the null hypothesis, it suggests that there is a significant difference or relationship between variables.

Alternative Hypothesis

An alternative hypothesis is a statement that assumes there is a significant difference or relationship between variables. It is often used as an alternative to the null hypothesis and is tested against the null hypothesis to determine which statement is more accurate.

Directional Hypothesis

A directional hypothesis is a statement that predicts the direction of the relationship between variables. For example, a researcher might predict that increasing the amount of exercise will result in a decrease in body weight.

Non-directional Hypothesis

A non-directional hypothesis is a statement that predicts the relationship between variables but does not specify the direction. For example, a researcher might predict that there is a relationship between the amount of exercise and body weight, but they do not specify whether increasing or decreasing exercise will affect body weight.

Statistical Hypothesis

A statistical hypothesis is a statement that assumes a particular statistical model or distribution for the data. It is often used in statistical analysis to test the significance of a particular result.

Composite Hypothesis

A composite hypothesis is a statement that assumes more than one condition or outcome. It can be divided into several sub-hypotheses, each of which represents a different possible outcome.

Empirical Hypothesis

An empirical hypothesis is a statement that is based on observed phenomena or data. It is often used in scientific research to develop theories or models that explain the observed phenomena.

Simple Hypothesis

A simple hypothesis is a statement that assumes only one outcome or condition. It is often used in scientific research to test a single variable or factor.

Complex Hypothesis

A complex hypothesis is a statement that assumes multiple outcomes or conditions. It is often used in scientific research to test the effects of multiple variables or factors on a particular outcome.

Applications of Hypothesis

Hypotheses are used in various fields to guide research and make predictions about the outcomes of experiments or observations. Here are some examples of how hypotheses are applied in different fields:

  • Science : In scientific research, hypotheses are used to test the validity of theories and models that explain natural phenomena. For example, a hypothesis might be formulated to test the effects of a particular variable on a natural system, such as the effects of climate change on an ecosystem.
  • Medicine : In medical research, hypotheses are used to test the effectiveness of treatments and therapies for specific conditions. For example, a hypothesis might be formulated to test the effects of a new drug on a particular disease.
  • Psychology : In psychology, hypotheses are used to test theories and models of human behavior and cognition. For example, a hypothesis might be formulated to test the effects of a particular stimulus on the brain or behavior.
  • Sociology : In sociology, hypotheses are used to test theories and models of social phenomena, such as the effects of social structures or institutions on human behavior. For example, a hypothesis might be formulated to test the effects of income inequality on crime rates.
  • Business : In business research, hypotheses are used to test the validity of theories and models that explain business phenomena, such as consumer behavior or market trends. For example, a hypothesis might be formulated to test the effects of a new marketing campaign on consumer buying behavior.
  • Engineering : In engineering, hypotheses are used to test the effectiveness of new technologies or designs. For example, a hypothesis might be formulated to test the efficiency of a new solar panel design.

How to write a Hypothesis

Here are the steps to follow when writing a hypothesis:

Identify the Research Question

The first step is to identify the research question that you want to answer through your study. This question should be clear, specific, and focused. It should be something that can be investigated empirically and that has some relevance or significance in the field.

Conduct a Literature Review

Before writing your hypothesis, it’s essential to conduct a thorough literature review to understand what is already known about the topic. This will help you to identify the research gap and formulate a hypothesis that builds on existing knowledge.

Determine the Variables

The next step is to identify the variables involved in the research question. A variable is any characteristic or factor that can vary or change. There are two types of variables: independent and dependent. The independent variable is the one that is manipulated or changed by the researcher, while the dependent variable is the one that is measured or observed as a result of the independent variable.

Formulate the Hypothesis

Based on the research question and the variables involved, you can now formulate your hypothesis. A hypothesis should be a clear and concise statement that predicts the relationship between the variables. It should be testable through empirical research and based on existing theory or evidence.

Write the Null Hypothesis

The null hypothesis is the opposite of the alternative hypothesis, which is the hypothesis that you are testing. The null hypothesis states that there is no significant difference or relationship between the variables. It is important to write the null hypothesis because it allows you to compare your results with what would be expected by chance.

Refine the Hypothesis

After formulating the hypothesis, it’s important to refine it and make it more precise. This may involve clarifying the variables, specifying the direction of the relationship, or making the hypothesis more testable.

Examples of Hypothesis

Here are a few examples of hypotheses in different fields:

  • Psychology : “Increased exposure to violent video games leads to increased aggressive behavior in adolescents.”
  • Biology : “Higher levels of carbon dioxide in the atmosphere will lead to increased plant growth.”
  • Sociology : “Individuals who grow up in households with higher socioeconomic status will have higher levels of education and income as adults.”
  • Education : “Implementing a new teaching method will result in higher student achievement scores.”
  • Marketing : “Customers who receive a personalized email will be more likely to make a purchase than those who receive a generic email.”
  • Physics : “An increase in temperature will cause an increase in the volume of a gas, assuming all other variables remain constant.”
  • Medicine : “Consuming a diet high in saturated fats will increase the risk of developing heart disease.”

Purpose of Hypothesis

The purpose of a hypothesis is to provide a testable explanation for an observed phenomenon or a prediction of a future outcome based on existing knowledge or theories. A hypothesis is an essential part of the scientific method and helps to guide the research process by providing a clear focus for investigation. It enables scientists to design experiments or studies to gather evidence and data that can support or refute the proposed explanation or prediction.

The formulation of a hypothesis is based on existing knowledge, observations, and theories, and it should be specific, testable, and falsifiable. A specific hypothesis helps to define the research question, which is important in the research process as it guides the selection of an appropriate research design and methodology. Testability of the hypothesis means that it can be proven or disproven through empirical data collection and analysis. Falsifiability means that the hypothesis should be formulated in such a way that it can be proven wrong if it is incorrect.

In addition to guiding the research process, the testing of hypotheses can lead to new discoveries and advancements in scientific knowledge. When a hypothesis is supported by the data, it can be used to develop new theories or models to explain the observed phenomenon. When a hypothesis is not supported by the data, it can help to refine existing theories or prompt the development of new hypotheses to explain the phenomenon.

When to use Hypothesis

Here are some common situations in which hypotheses are used:

  • In scientific research , hypotheses are used to guide the design of experiments and to help researchers make predictions about the outcomes of those experiments.
  • In social science research , hypotheses are used to test theories about human behavior, social relationships, and other phenomena.
  • I n business , hypotheses can be used to guide decisions about marketing, product development, and other areas. For example, a hypothesis might be that a new product will sell well in a particular market, and this hypothesis can be tested through market research.

Characteristics of Hypothesis

Here are some common characteristics of a hypothesis:

  • Testable : A hypothesis must be able to be tested through observation or experimentation. This means that it must be possible to collect data that will either support or refute the hypothesis.
  • Falsifiable : A hypothesis must be able to be proven false if it is not supported by the data. If a hypothesis cannot be falsified, then it is not a scientific hypothesis.
  • Clear and concise : A hypothesis should be stated in a clear and concise manner so that it can be easily understood and tested.
  • Based on existing knowledge : A hypothesis should be based on existing knowledge and research in the field. It should not be based on personal beliefs or opinions.
  • Specific : A hypothesis should be specific in terms of the variables being tested and the predicted outcome. This will help to ensure that the research is focused and well-designed.
  • Tentative: A hypothesis is a tentative statement or assumption that requires further testing and evidence to be confirmed or refuted. It is not a final conclusion or assertion.
  • Relevant : A hypothesis should be relevant to the research question or problem being studied. It should address a gap in knowledge or provide a new perspective on the issue.

Advantages of Hypothesis

Hypotheses have several advantages in scientific research and experimentation:

  • Guides research: A hypothesis provides a clear and specific direction for research. It helps to focus the research question, select appropriate methods and variables, and interpret the results.
  • Predictive powe r: A hypothesis makes predictions about the outcome of research, which can be tested through experimentation. This allows researchers to evaluate the validity of the hypothesis and make new discoveries.
  • Facilitates communication: A hypothesis provides a common language and framework for scientists to communicate with one another about their research. This helps to facilitate the exchange of ideas and promotes collaboration.
  • Efficient use of resources: A hypothesis helps researchers to use their time, resources, and funding efficiently by directing them towards specific research questions and methods that are most likely to yield results.
  • Provides a basis for further research: A hypothesis that is supported by data provides a basis for further research and exploration. It can lead to new hypotheses, theories, and discoveries.
  • Increases objectivity: A hypothesis can help to increase objectivity in research by providing a clear and specific framework for testing and interpreting results. This can reduce bias and increase the reliability of research findings.

Limitations of Hypothesis

Some Limitations of the Hypothesis are as follows:

  • Limited to observable phenomena: Hypotheses are limited to observable phenomena and cannot account for unobservable or intangible factors. This means that some research questions may not be amenable to hypothesis testing.
  • May be inaccurate or incomplete: Hypotheses are based on existing knowledge and research, which may be incomplete or inaccurate. This can lead to flawed hypotheses and erroneous conclusions.
  • May be biased: Hypotheses may be biased by the researcher’s own beliefs, values, or assumptions. This can lead to selective interpretation of data and a lack of objectivity in research.
  • Cannot prove causation: A hypothesis can only show a correlation between variables, but it cannot prove causation. This requires further experimentation and analysis.
  • Limited to specific contexts: Hypotheses are limited to specific contexts and may not be generalizable to other situations or populations. This means that results may not be applicable in other contexts or may require further testing.
  • May be affected by chance : Hypotheses may be affected by chance or random variation, which can obscure or distort the true relationship between variables.

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Examples

Biology Hypothesis

hypothesis definition biology examples

Delve into the fascinating world of biology with our definitive guide on crafting impeccable hypothesis thesis statements . As the foundation of any impactful biological research, a well-formed hypothesis paves the way for groundbreaking discoveries and insights. Whether you’re examining cellular behavior or large-scale ecosystems, mastering the art of the thesis statement is crucial. Embark on this enlightening journey with us, as we provide stellar examples and invaluable writing advice tailored for budding biologists.

What is a good hypothesis in biology?

A good hypothesis in biology is a statement that offers a tentative explanation for a biological phenomenon, based on prior knowledge or observation. It should be:

  • Testable: The hypothesis should be measurable and can be proven false through experiments or observations.
  • Clear: It should be stated clearly and without ambiguity.
  • Based on Knowledge: A solid hypothesis often stems from existing knowledge or literature in the field.
  • Specific: It should clearly define the variables being tested and the expected outcomes.
  • Falsifiable: It’s essential that a hypothesis can be disproven. This means there should be a possible result that could indicate the hypothesis is incorrect.

What is an example of a hypothesis statement in biology?

Example: “If a plant is given a higher concentration of carbon dioxide, then it will undergo photosynthesis at an increased rate compared to a plant given a standard concentration of carbon dioxide.”

In this example:

  • The independent variable (what’s being changed) is the concentration of carbon dioxide.
  • The dependent variable (what’s being measured) is the rate of photosynthesis. The statement proposes a cause-and-effect relationship that can be tested through experimentation.

100 Biology Thesis Statement Examples

Biology Thesis Statement Examples

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Biology, as the study of life and living organisms, is vast and diverse. Crafting a good thesis statement in this field requires a clear understanding of the topic at hand, capturing the essence of the research aim. From genetics to ecology, from cell biology to animal behavior, the following examples will give you a comprehensive idea about forming succinct biology thesis statements.

Genetics: Understanding the role of the BRCA1 gene in breast cancer susceptibility can lead to targeted treatments.

2. Evolution: The finch populations of the Galápagos Islands provide evidence of natural selection through beak variations in response to food availability.

3. Cell Biology: Mitochondrial dysfunction is a central factor in the onset of age-related neurodegenerative diseases.

4. Ecology: Deforestation in the Amazon directly impacts global carbon dioxide levels, influencing climate change.

5. Human Anatomy: Regular exercise enhances cardiovascular health by improving heart muscle function and reducing arterial plaque.

6. Marine Biology: Coral bleaching events in the Great Barrier Reef correlate strongly with rising sea temperatures.

7. Zoology: Migration patterns of Monarch butterflies are influenced by seasonal changes and available food sources.

8. Botany: The symbiotic relationship between mycorrhizal fungi and plant roots enhances nutrient absorption in poor soil conditions.

9. Microbiology: The overuse of antibiotics in healthcare has accelerated the evolution of antibiotic-resistant bacterial strains.

10. Physiology: High altitude adaptation in certain human populations has led to increased hemoglobin production.

11. Immunology: The role of T-cells in the human immune response is critical in developing effective vaccines against viral diseases.

12. Behavioral Biology: Birdsong variations in sparrows can be attributed to both genetic factors and environmental influences.

13. Developmental Biology: The presence of certain hormones during fetal development dictates the differentiation of sex organs in mammals.

14. Conservation Biology: The rapid decline of bee populations worldwide is directly linked to the use of certain pesticides in agriculture.

15. Molecular Biology: The CRISPR-Cas9 system has revolutionized gene editing techniques, offering potential cures for genetic diseases.

16. Virology: The mutation rate of the influenza virus necessitates annual updates in vaccine formulations.

17. Neurobiology: Neural plasticity in the adult brain can be enhanced through consistent learning and cognitive challenges.

18. Ethology: Elephant herds exhibit complex social structures and matriarchal leadership.

19. Biotechnology: Genetically modified crops can improve yield and resistance but also pose ecological challenges.

20. Environmental Biology: Industrial pollution in freshwater systems disrupts aquatic life and can lead to loss of biodiversity.

21. Neurodegenerative Diseases: Amyloid-beta protein accumulation in the brain is a key marker for Alzheimer’s disease progression.

22. Endocrinology: The disruption of thyroid hormone balance leads to metabolic disorders and weight fluctuations.

23. Bioinformatics: Machine learning algorithms can predict protein structures with high accuracy, advancing drug design.

24. Plant Physiology: The stomatal closure mechanism in plants helps prevent water loss and maintain turgor pressure.

25. Parasitology: The lifecycle of the malaria parasite involves complex interactions between humans and mosquitoes.

26. Molecular Genetics: Epigenetic modifications play a crucial role in gene expression regulation and cell differentiation.

27. Evolutionary Psychology: Human preference for symmetrical faces is a result of evolutionarily advantageous traits.

28. Ecosystem Dynamics: The reintroduction of apex predators in ecosystems restores ecological balance and biodiversity.

29. Epigenetics: Maternal dietary choices during pregnancy can influence the epigenetic profiles of offspring.

30. Biochemistry: Enzyme kinetics in metabolic pathways reveal insights into cellular energy production.

31. Bioluminescence: The role of bioluminescence in deep-sea organisms serves as camouflage and communication.

32. Genetics of Disease: Mutations in the CFTR gene cause cystic fibrosis, leading to severe respiratory and digestive issues.

33. Reproductive Biology: The influence of pheromones on mate selection is a critical aspect of reproductive success in many species.

34. Plant-Microbe Interactions: Rhizobium bacteria facilitate nitrogen fixation in leguminous plants, benefiting both organisms.

35. Comparative Anatomy: Homologous structures in different species provide evidence of shared evolutionary ancestry.

36. Stem Cell Research: Induced pluripotent stem cells hold immense potential for regenerative medicine and disease modeling.

37. Bioethics: Balancing the use of genetic modification in humans with ethical considerations is a complex challenge.

38. Molecular Evolution: The study of orthologous and paralogous genes offers insights into evolutionary relationships.

39. Bioenergetics: ATP synthesis through oxidative phosphorylation is a fundamental process driving cellular energy production.

40. Population Genetics: The Hardy-Weinberg equilibrium model helps predict allele frequencies in populations over time.

41. Animal Communication: The complex vocalizations of whales serve both social bonding and long-distance communication purposes.

42. Biogeography: The distribution of marsupials in Australia and their absence elsewhere highlights the impact of geographical isolation on evolution.

43. Aquatic Ecology: The phenomenon of eutrophication in lakes is driven by excessive nutrient runoff and results in harmful algal blooms.

44. Insect Behavior: The waggle dance of honeybees conveys precise information about the location of food sources to other members of the hive.

45. Microbial Ecology: The gut microbiome’s composition influences host health, metabolism, and immune system development.

46. Evolution of Sex: The Red Queen hypothesis explains the evolution of sexual reproduction as a defense against rapidly evolving parasites.

47. Immunotherapy: Manipulating the immune response to target cancer cells shows promise as an effective cancer treatment strategy.

48. Epigenetic Inheritance: Epigenetic modifications can be passed down through generations, impacting traits and disease susceptibility.

49. Comparative Genomics: Comparing the genomes of different species sheds light on genetic adaptations and evolutionary divergence.

50. Neurotransmission: The dopamine reward pathway in the brain is implicated in addiction and motivation-related behaviors.

51. Microbial Biotechnology: Genetically engineered bacteria can produce valuable compounds like insulin, revolutionizing pharmaceutical production.

52. Bioinformatics: DNA sequence analysis reveals evolutionary relationships between species and uncovers hidden genetic information.

53. Animal Migration: The navigational abilities of migratory birds are influenced by magnetic fields and celestial cues.

54. Human Evolution: The discovery of ancient hominin fossils provides insights into the evolutionary timeline of our species.

55. Cancer Genetics: Mutations in tumor suppressor genes contribute to the uncontrolled growth and division of cancer cells.

56. Aquatic Biomes: Coral reefs, rainforests of the sea, host incredible biodiversity and face threats from climate change and pollution.

57. Genomic Medicine: Personalized treatments based on an individual’s genetic makeup hold promise for more effective healthcare.

58. Molecular Pharmacology: Understanding receptor-ligand interactions aids in the development of targeted drugs for specific diseases.

59. Biodiversity Conservation: Preserving habitat diversity is crucial to maintaining ecosystems and preventing species extinction.

60. Evolutionary Developmental Biology: Comparing embryonic development across species reveals shared genetic pathways and evolutionary constraints.

61. Plant Reproductive Strategies: Understanding the trade-offs between asexual and sexual reproduction in plants sheds light on their evolutionary success.

62. Parasite-Host Interactions: The coevolution of parasites and their hosts drives adaptations and counter-adaptations over time.

63. Genomic Diversity: Exploring genetic variations within populations helps uncover disease susceptibilities and evolutionary history.

64. Ecological Succession: Studying the process of ecosystem recovery after disturbances provides insights into resilience and stability.

65. Conservation Genetics: Genetic diversity assessment aids in formulating effective conservation strategies for endangered species.

66. Neuroplasticity and Learning: Investigating how the brain adapts through synaptic changes improves our understanding of memory and learning.

67. Synthetic Biology: Designing and engineering biological systems offers innovative solutions for medical, environmental, and industrial challenges.

68. Ethnobotany: Documenting the traditional uses of plants by indigenous communities informs both conservation and pharmaceutical research.

69. Ecological Niche Theory: Exploring how species adapt to specific ecological niches enhances our grasp of biodiversity patterns.

70. Ecosystem Services: Quantifying the benefits provided by ecosystems, like pollination and carbon sequestration, supports conservation efforts.

71. Fungal Biology: Investigating mycorrhizal relationships between fungi and plants illuminates nutrient exchange mechanisms.

72. Molecular Clock Hypothesis: Genetic mutations accumulate over time, providing a method to estimate evolutionary divergence dates.

73. Developmental Disorders: Unraveling the genetic and environmental factors contributing to developmental disorders informs therapeutic approaches.

74. Epigenetics and Disease: Epigenetic modifications contribute to the development of diseases like cancer, diabetes, and neurodegenerative disorders.

75. Animal Cognition: Studying cognitive abilities in animals unveils their problem-solving skills, social dynamics, and sensory perceptions.

76. Microbiota-Brain Axis: The gut-brain connection suggests a bidirectional communication pathway influencing mental health and behavior.

77. Neurological Disorders: Neurodegenerative diseases like Parkinson’s and Alzheimer’s have genetic and environmental components that drive their progression.

78. Plant Defense Mechanisms: Investigating how plants ward off pests and pathogens informs sustainable agricultural practices.

79. Conservation Genomics: Genetic data aids in identifying distinct populations and prioritizing conservation efforts for at-risk species.

80. Reproductive Strategies: Comparing reproductive methods in different species provides insights into evolutionary trade-offs and reproductive success.

81. Epigenetics in Aging: Exploring epigenetic changes in the aging process offers insights into longevity and age-related diseases.

82. Antimicrobial Resistance: Understanding the genetic mechanisms behind bacterial resistance to antibiotics informs strategies to combat the global health threat.

83. Plant-Animal Interactions: Investigating mutualistic relationships between plants and pollinators showcases the delicate balance of ecosystems.

84. Adaptations to Extreme Environments: Studying extremophiles reveals the remarkable ways organisms thrive in extreme conditions like deep-sea hydrothermal vents.

85. Genetic Disorders: Genetic mutations underlie numerous disorders like cystic fibrosis, sickle cell anemia, and muscular dystrophy.

86. Conservation Behavior: Analyzing the behavioral ecology of endangered species informs habitat preservation and restoration efforts.

87. Neuroplasticity in Rehabilitation: Harnessing the brain’s ability to rewire itself offers promising avenues for post-injury or post-stroke rehabilitation.

88. Disease Vectors: Understanding how mosquitoes transmit diseases like malaria and Zika virus is critical for disease prevention strategies.

89. Biochemical Pathways: Mapping metabolic pathways in cells provides insights into disease development and potential therapeutic targets.

90. Invasive Species Impact: Examining the effects of invasive species on native ecosystems guides management strategies to mitigate their impact.

91. Molecular Immunology: Studying the intricate immune response mechanisms aids in the development of vaccines and immunotherapies.

92. Plant-Microbe Symbiosis: Investigating how plants form partnerships with beneficial microbes enhances crop productivity and sustainability.

93. Cancer Immunotherapy: Harnessing the immune system to target and eliminate cancer cells offers new avenues for cancer treatment.

94. Evolution of Flight: Analyzing the adaptations leading to the development of flight in birds and insects sheds light on evolutionary innovation.

95. Genomic Diversity in Human Populations: Exploring genetic variations among different human populations informs ancestry, migration, and susceptibility to diseases.

96. Hormonal Regulation: Understanding the role of hormones in growth, reproduction, and homeostasis provides insights into physiological processes.

97. Conservation Genetics in Plant Conservation: Genetic diversity assessment helps guide efforts to conserve rare and endangered plant species.

98. Neuronal Communication: Investigating neurotransmitter systems and synaptic transmission enhances our comprehension of brain function.

99. Microbial Biogeography: Mapping the distribution of microorganisms across ecosystems aids in understanding their ecological roles and interactions.

100. Gene Therapy: Developing methods to replace or repair defective genes offers potential treatments for genetic disorders.

Scientific Hypothesis Statement Examples

This section offers diverse examples of scientific hypothesis statements that cover a range of biological topics. Each example briefly describes the subject matter and the potential implications of the hypothesis.

  • Genetic Mutations and Disease: Certain genetic mutations lead to increased susceptibility to autoimmune disorders, providing insights into potential treatment strategies.
  • Microplastics in Aquatic Ecosystems: Elevated microplastic levels disrupt aquatic food chains, affecting biodiversity and human health through bioaccumulation.
  • Bacterial Quorum Sensing: Inhibition of quorum sensing in pathogenic bacteria demonstrates a potential avenue for novel antimicrobial therapies.
  • Climate Change and Phenology: Rising temperatures alter flowering times in plants, impacting pollinator interactions and ecosystem dynamics.
  • Neuroplasticity and Learning: The brain’s adaptability facilitates learning through synaptic modifications, elucidating educational strategies for improved cognition.
  • CRISPR-Cas9 in Agriculture: CRISPR-engineered crops with enhanced pest resistance showcase a sustainable approach to improving agricultural productivity.
  • Invasive Species Impact on Predators: The introduction of invasive prey disrupts predator-prey relationships, triggering cascading effects in terrestrial ecosystems.
  • Microbial Contributions to Soil Health: Beneficial soil microbes enhance nutrient availability and plant growth, promoting sustainable agriculture practices.
  • Marine Protected Areas: Examining the effectiveness of marine protected areas reveals their role in preserving biodiversity and restoring marine ecosystems.
  • Epigenetic Regulation of Cancer: Epigenetic modifications play a pivotal role in cancer development, highlighting potential therapeutic targets for precision medicine.

Testable Hypothesis Statement Examples in Biology

Testability hypothesis is a critical aspect of a hypothesis. These examples are formulated in a way that allows them to be tested through experiments or observations. They focus on cause-and-effect relationships that can be verified or refuted.

  • Impact of Light Intensity on Plant Growth: Increasing light intensity accelerates photosynthesis rates and enhances overall plant growth.
  • Effect of Temperature on Enzyme Activity: Higher temperatures accelerate enzyme activity up to an optimal point, beyond which denaturation occurs.
  • Microbial Diversity in Soil pH Gradients: Soil pH influences microbial composition, with acidic soils favoring certain bacterial taxa over others.
  • Predation Impact on Prey Behavior: The presence of predators induces changes in prey behavior, resulting in altered foraging strategies and vigilance levels.
  • Chemical Communication in Marine Organisms: Investigating chemical cues reveals the role of allelopathy in competition among marine organisms.
  • Social Hierarchy in Animal Groups: Observing animal groups establishes a correlation between social rank and access to resources within the group.
  • Effect of Habitat Fragmentation on Pollinator Diversity: Fragmented habitats reduce pollinator species richness, affecting plant reproductive success.
  • Dietary Effects on Gut Microbiota Composition: Dietary shifts influence gut microbiota diversity and metabolic functions, impacting host health.
  • Hybridization Impact on Plant Fitness: Hybrid plants exhibit varied fitness levels depending on the combination of parent species.
  • Human Impact on Coral Bleaching: Analyzing coral reefs under different anthropogenic stresses identifies the main factors driving coral bleaching events.

Scientific Investigation Hypothesis Statement Examples in Biology

This section emphasizes hypotheses that are part of broader scientific investigations. They involve studying complex interactions or phenomena and often contribute to our understanding of larger biological systems.

  • Genomic Variation in Human Disease Susceptibility: Genetic analysis identifies variations associated with increased risk of common diseases, aiding personalized medicine.
  • Behavioral Responses to Temperature Shifts in Insects: Investigating insect responses to temperature fluctuations reveals adaptation strategies to climate change.
  • Endocrine Disruptors and Amphibian Development: Experimental exposure to endocrine disruptors elucidates their role in amphibian developmental abnormalities.
  • Microbial Succession in Decomposition: Tracking microbial communities during decomposition uncovers the succession patterns of different decomposer species.
  • Gene Expression Patterns in Stress Response: Studying gene expression profiles unveils the molecular mechanisms underlying stress responses in plants.
  • Effect of Urbanization on Bird Song Patterns: Urban noise pollution influences bird song frequency and complexity, impacting communication and mate attraction.
  • Nutrient Availability and Algal Blooms: Investigating nutrient loading in aquatic systems sheds light on factors triggering harmful algal blooms.
  • Host-Parasite Coevolution: Analyzing genetic changes in hosts and parasites over time uncovers coevolutionary arms races and adaptation.
  • Ecosystem Productivity and Biodiversity: Linking ecosystem productivity to biodiversity patterns reveals the role of species interactions in ecosystem stability.
  • Habitat Preference of Invasive Species: Studying the habitat selection of invasive species identifies factors promoting their establishment and spread.

Hypothesis Statement Examples in Biology Research

These examples are tailored for research hypothesis studies. They highlight hypotheses that drive focused research questions, often leading to specific experimental designs and data collection methods.

  • Microbial Community Structure in Human Gut: Investigating microbial diversity and composition unveils the role of gut microbiota in human health.
  • Plant-Pollinator Mutualisms: Hypothesizing reciprocal benefits in plant-pollinator interactions highlights the role of coevolution in shaping ecosystems.
  • Chemical Defense Mechanisms in Insects: Predicting the correlation between insect feeding behavior and chemical defenses explores natural selection pressures.
  • Evolutionary Significance of Mimicry: Examining mimicry in organisms demonstrates its adaptive value in predator-prey relationships and survival.
  • Neurological Basis of Mate Choice: Proposing neural mechanisms underlying mate choice behaviors uncovers the role of sensory cues in reproductive success.
  • Mycorrhizal Symbiosis Impact on Plant Growth: Investigating mycorrhizal colonization effects on plant biomass addresses nutrient exchange dynamics.
  • Social Learning in Primates: Formulating a hypothesis on primate social learning explores the transmission of knowledge and cultural behaviors.
  • Effect of Pollution on Fish Behavior: Anticipating altered behaviors due to pollution exposure highlights ecological consequences on aquatic ecosystems.
  • Coevolution of Flowers and Pollinators: Hypothesizing mutual adaptations between flowers and pollinators reveals intricate ecological relationships.
  • Genetic Basis of Disease Resistance in Plants: Identifying genetic markers associated with disease resistance enhances crop breeding programs.

Prediction Hypothesis Statement Examples in Biology

Predictive simple hypothesis involve making educated guesses about how variables might interact or behave under specific conditions. These examples showcase hypotheses that anticipate outcomes based on existing knowledge.

  • Pesticide Impact on Insect Abundance: Predicting decreased insect populations due to pesticide application underscores ecological ramifications.
  • Climate Change and Migratory Bird Patterns: Anticipating shifts in migratory routes of birds due to climate change informs conservation strategies.
  • Ocean Acidification Effect on Coral Calcification: Predicting reduced coral calcification rates due to ocean acidification unveils threats to coral reefs.
  • Disease Spread in Crowded Bird Roosts: Predicting accelerated disease transmission in densely populated bird roosts highlights disease ecology dynamics.
  • Eutrophication Impact on Freshwater Biodiversity: Anticipating decreased freshwater biodiversity due to eutrophication emphasizes conservation efforts.
  • Herbivore Impact on Plant Species Diversity: Predicting reduced plant diversity in areas with high herbivore pressure elucidates ecosystem dynamics.
  • Predator-Prey Population Cycles: Predicting cyclical fluctuations in predator and prey populations showcases the role of trophic interactions.
  • Climate Change and Plant Phenology: Anticipating earlier flowering times due to climate change demonstrates the influence of temperature on plant life cycles.
  • Antibiotic Resistance in Bacterial Communities: Predicting increased antibiotic resistance due to overuse forewarns the need for responsible antibiotic use.
  • Human Impact on Avian Nesting Success: Predicting decreased avian nesting success due to habitat fragmentation highlights conservation priorities.

How to Write a Biology Hypothesis – Step by Step Guide

A hypothesis in biology is a critical component of scientific research that proposes an explanation for a specific biological phenomenon. Writing a well-formulated hypothesis sets the foundation for conducting experiments, making observations, and drawing meaningful conclusions. Follow this step-by-step guide to create a strong biology hypothesis:

1. Identify the Phenomenon: Clearly define the biological phenomenon you intend to study. This could be a question, a pattern, an observation, or a problem in the field of biology.

2. Conduct Background Research: Before formulating a hypothesis, gather relevant information from scientific literature. Understand the existing knowledge about the topic to ensure your hypothesis builds upon previous research.

3. State the Independent and Dependent Variables: Identify the variables involved in the phenomenon. The independent variable is what you manipulate or change, while the dependent variable is what you measure as a result of the changes.

4. Formulate a Testable Question: Based on your background research, create a specific and testable question that addresses the relationship between the variables. This question will guide the formulation of your hypothesis.

5. Craft the Hypothesis: A hypothesis should be a clear and concise statement that predicts the outcome of your experiment or observation. It should propose a cause-and-effect relationship between the independent and dependent variables.

6. Use the “If-Then” Structure: Formulate your hypothesis using the “if-then” structure. The “if” part states the independent variable and the condition you’re manipulating, while the “then” part predicts the outcome for the dependent variable.

7. Make it Falsifiable: A good hypothesis should be testable and capable of being proven false. There should be a way to gather data that either supports or contradicts the hypothesis.

8. Be Specific and Precise: Avoid vague language and ensure that your hypothesis is specific and precise. Clearly define the variables and the expected relationship between them.

9. Revise and Refine: Once you’ve formulated your hypothesis, review it to ensure it accurately reflects your research question and variables. Revise as needed to make it more concise and focused.

10. Seek Feedback: Share your hypothesis with peers, mentors, or colleagues to get feedback. Constructive input can help you refine your hypothesis further.

Tips for Writing a Biology Hypothesis Statement

Writing a biology alternative hypothesis statement requires precision and clarity to ensure that your research is well-structured and testable. Here are some valuable tips to help you create effective and scientifically sound hypothesis statements:

1. Be Clear and Concise: Your hypothesis statement should convey your idea succinctly. Avoid unnecessary jargon or complex language that might confuse your audience.

2. Address Cause and Effect: A hypothesis suggests a cause-and-effect relationship between variables. Clearly state how changes in the independent variable are expected to affect the dependent variable.

3. Use Specific Language: Define your variables precisely. Use specific terms to describe the independent and dependent variables, as well as any conditions or measurements.

4. Follow the “If-Then” Structure: Use the classic “if-then” structure to frame your hypothesis. State the independent variable (if) and the expected outcome (then). This format clarifies the relationship you’re investigating.

5. Make it Testable: Your hypothesis must be capable of being tested through experimentation or observation. Ensure that there is a measurable and observable way to determine if it’s true or false.

6. Avoid Ambiguity: Eliminate vague terms that can be interpreted in multiple ways. Be precise in your language to avoid confusion.

7. Base it on Existing Knowledge: Ground your hypothesis in prior research or existing scientific theories. It should build upon established knowledge and contribute new insights.

8. Predict a Direction: Your hypothesis should predict a specific outcome. Whether you anticipate an increase, decrease, or a difference, your hypothesis should make a clear prediction.

9. Be Focused: Keep your hypothesis statement focused on one specific idea or relationship. Avoid trying to address too many variables or concepts in a single statement.

10. Consider Alternative Explanations: Acknowledge alternative explanations for your observations or outcomes. This demonstrates critical thinking and a thorough understanding of your field.

11. Avoid Value Judgments: Refrain from including value judgments or opinions in your hypothesis. Stick to objective and measurable factors.

12. Be Realistic: Ensure that your hypothesis is plausible and feasible. It should align with what is known about the topic and be achievable within the scope of your research.

13. Refine and Revise: Draft multiple versions of your hypothesis statement and refine them. Discuss and seek feedback from mentors, peers, or advisors to enhance its clarity and precision.

14. Align with Research Goals: Your hypothesis should align with the overall goals of your research project. Make sure it addresses the specific question or problem you’re investigating.

15. Be Open to Revision: As you conduct research and gather data, be open to revising your hypothesis if the evidence suggests a different outcome than initially predicted.

Remember, a well-crafted biology science hypothesis statement serves as the foundation of your research and guides your experimental design and data analysis. It’s essential to invest time and effort in formulating a clear, focused, and testable hypothesis that contributes to the advancement of scientific knowledge.

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How to Write a Hypothesis? Types and Examples 

how to write a hypothesis for research

All research studies involve the use of the scientific method, which is a mathematical and experimental technique used to conduct experiments by developing and testing a hypothesis or a prediction about an outcome. Simply put, a hypothesis is a suggested solution to a problem. It includes elements that are expressed in terms of relationships with each other to explain a condition or an assumption that hasn’t been verified using facts. 1 The typical steps in a scientific method include developing such a hypothesis, testing it through various methods, and then modifying it based on the outcomes of the experiments.  

A research hypothesis can be defined as a specific, testable prediction about the anticipated results of a study. 2 Hypotheses help guide the research process and supplement the aim of the study. After several rounds of testing, hypotheses can help develop scientific theories. 3 Hypotheses are often written as if-then statements. 

Here are two hypothesis examples: 

Dandelions growing in nitrogen-rich soils for two weeks develop larger leaves than those in nitrogen-poor soils because nitrogen stimulates vegetative growth. 4  

If a company offers flexible work hours, then their employees will be happier at work. 5  

Table of Contents

  • What is a hypothesis? 
  • Types of hypotheses 
  • Characteristics of a hypothesis 
  • Functions of a hypothesis 
  • How to write a hypothesis 
  • Hypothesis examples 
  • Frequently asked questions 

What is a hypothesis?

Figure 1. Steps in research design

A hypothesis expresses an expected relationship between variables in a study and is developed before conducting any research. Hypotheses are not opinions but rather are expected relationships based on facts and observations. They help support scientific research and expand existing knowledge. An incorrectly formulated hypothesis can affect the entire experiment leading to errors in the results so it’s important to know how to formulate a hypothesis and develop it carefully.

A few sources of a hypothesis include observations from prior studies, current research and experiences, competitors, scientific theories, and general conditions that can influence people. Figure 1 depicts the different steps in a research design and shows where exactly in the process a hypothesis is developed. 4  

There are seven different types of hypotheses—simple, complex, directional, nondirectional, associative and causal, null, and alternative. 

Types of hypotheses

The seven types of hypotheses are listed below: 5 , 6,7  

  • Simple : Predicts the relationship between a single dependent variable and a single independent variable. 

Example: Exercising in the morning every day will increase your productivity.  

  • Complex : Predicts the relationship between two or more variables. 

Example: Spending three hours or more on social media daily will negatively affect children’s mental health and productivity, more than that of adults.  

  • Directional : Specifies the expected direction to be followed and uses terms like increase, decrease, positive, negative, more, or less. 

Example: The inclusion of intervention X decreases infant mortality compared to the original treatment.  

  • Non-directional : Does not predict the exact direction, nature, or magnitude of the relationship between two variables but rather states the existence of a relationship. This hypothesis may be used when there is no underlying theory or if findings contradict prior research. 

Example: Cats and dogs differ in the amount of affection they express.  

  • Associative and causal : An associative hypothesis suggests an interdependency between variables, that is, how a change in one variable changes the other.  

Example: There is a positive association between physical activity levels and overall health.  

A causal hypothesis, on the other hand, expresses a cause-and-effect association between variables. 

Example: Long-term alcohol use causes liver damage.  

  • Null : Claims that the original hypothesis is false by showing that there is no relationship between the variables. 

Example: Sleep duration does not have any effect on productivity.  

  • Alternative : States the opposite of the null hypothesis, that is, a relationship exists between two variables. 

Example: Sleep duration affects productivity.  

hypothesis definition biology examples

Characteristics of a hypothesis

So, what makes a good hypothesis? Here are some important characteristics of a hypothesis. 8,9  

  • Testable : You must be able to test the hypothesis using scientific methods to either accept or reject the prediction. 
  • Falsifiable : It should be possible to collect data that reject rather than support the hypothesis. 
  • Logical : Hypotheses shouldn’t be a random guess but rather should be based on previous theories, observations, prior research, and logical reasoning. 
  • Positive : The hypothesis statement about the existence of an association should be positive, that is, it should not suggest that an association does not exist. Therefore, the language used and knowing how to phrase a hypothesis is very important. 
  • Clear and accurate : The language used should be easily comprehensible and use correct terminology. 
  • Relevant : The hypothesis should be relevant and specific to the research question. 
  • Structure : Should include all the elements that make a good hypothesis: variables, relationship, and outcome. 

Functions of a hypothesis

The following list mentions some important functions of a hypothesis: 1  

  • Maintains the direction and progress of the research. 
  • Expresses the important assumptions underlying the proposition in a single statement. 
  • Establishes a suitable context for researchers to begin their investigation and for readers who are referring to the final report. 
  • Provides an explanation for the occurrence of a specific phenomenon. 
  • Ensures selection of appropriate and accurate facts necessary and relevant to the research subject. 

To summarize, a hypothesis provides the conceptual elements that complete the known data, conceptual relationships that systematize unordered elements, and conceptual meanings and interpretations that explain the unknown phenomena. 1  

hypothesis definition biology examples

How to write a hypothesis

Listed below are the main steps explaining how to write a hypothesis. 2,4,5  

  • Make an observation and identify variables : Observe the subject in question and try to recognize a pattern or a relationship between the variables involved. This step provides essential background information to begin your research.  

For example, if you notice that an office’s vending machine frequently runs out of a specific snack, you may predict that more people in the office choose that snack over another. 

  • Identify the main research question : After identifying a subject and recognizing a pattern, the next step is to ask a question that your hypothesis will answer.  

For example, after observing employees’ break times at work, you could ask “why do more employees take breaks in the morning rather than in the afternoon?” 

  • Conduct some preliminary research to ensure originality and novelty : Your initial answer, which is your hypothesis, to the question is based on some pre-existing information about the subject. However, to ensure that your hypothesis has not been asked before or that it has been asked but rejected by other researchers you would need to gather additional information.  

For example, based on your observations you might state a hypothesis that employees work more efficiently when the air conditioning in the office is set at a lower temperature. However, during your preliminary research you find that this hypothesis was proven incorrect by a prior study. 

  • Develop a general statement : After your preliminary research has confirmed the originality of your proposed answer, draft a general statement that includes all variables, subjects, and predicted outcome. The statement could be if/then or declarative.  
  • Finalize the hypothesis statement : Use the PICOT model, which clarifies how to word a hypothesis effectively, when finalizing the statement. This model lists the important components required to write a hypothesis. 

P opulation: The specific group or individual who is the main subject of the research 

I nterest: The main concern of the study/research question 

C omparison: The main alternative group 

O utcome: The expected results  

T ime: Duration of the experiment 

Once you’ve finalized your hypothesis statement you would need to conduct experiments to test whether the hypothesis is true or false. 

Hypothesis examples

The following table provides examples of different types of hypotheses. 10 ,11  

hypothesis definition biology examples

Key takeaways  

Here’s a summary of all the key points discussed in this article about how to write a hypothesis. 

  • A hypothesis is an assumption about an association between variables made based on limited evidence, which should be tested. 
  • A hypothesis has four parts—the research question, independent variable, dependent variable, and the proposed relationship between the variables.   
  • The statement should be clear, concise, testable, logical, and falsifiable. 
  • There are seven types of hypotheses—simple, complex, directional, non-directional, associative and causal, null, and alternative. 
  • A hypothesis provides a focus and direction for the research to progress. 
  • A hypothesis plays an important role in the scientific method by helping to create an appropriate experimental design. 

Frequently asked questions

Hypotheses and research questions have different objectives and structure. The following table lists some major differences between the two. 9  

Here are a few examples to differentiate between a research question and hypothesis. 

Yes, here’s a simple checklist to help you gauge the effectiveness of your hypothesis. 9   1. When writing a hypothesis statement, check if it:  2. Predicts the relationship between the stated variables and the expected outcome.  3. Uses simple and concise language and is not wordy.  4. Does not assume readers’ knowledge about the subject.  5. Has observable, falsifiable, and testable results. 

As mentioned earlier in this article, a hypothesis is an assumption or prediction about an association between variables based on observations and simple evidence. These statements are usually generic. Research objectives, on the other hand, are more specific and dictated by hypotheses. The same hypothesis can be tested using different methods and the research objectives could be different in each case.     For example, Louis Pasteur observed that food lasts longer at higher altitudes, reasoned that it could be because the air at higher altitudes is cleaner (with fewer or no germs), and tested the hypothesis by exposing food to air cleaned in the laboratory. 12 Thus, a hypothesis is predictive—if the reasoning is correct, X will lead to Y—and research objectives are developed to test these predictions. 

Null hypothesis testing is a method to decide between two assumptions or predictions between variables (null and alternative hypotheses) in a statistical relationship in a sample. The null hypothesis, denoted as H 0 , claims that no relationship exists between variables in a population and any relationship in the sample reflects a sampling error or occurrence by chance. The alternative hypothesis, denoted as H 1 , claims that there is a relationship in the population. In every study, researchers need to decide whether the relationship in a sample occurred by chance or reflects a relationship in the population. This is done by hypothesis testing using the following steps: 13   1. Assume that the null hypothesis is true.  2. Determine how likely the sample relationship would be if the null hypothesis were true. This probability is called the p value.  3. If the sample relationship would be extremely unlikely, reject the null hypothesis and accept the alternative hypothesis. If the relationship would not be unlikely, accept the null hypothesis. 

hypothesis definition biology examples

To summarize, researchers should know how to write a good hypothesis to ensure that their research progresses in the required direction. A hypothesis is a testable prediction about any behavior or relationship between variables, usually based on facts and observation, and states an expected outcome.  

We hope this article has provided you with essential insight into the different types of hypotheses and their functions so that you can use them appropriately in your next research project. 

References  

  • Dalen, DVV. The function of hypotheses in research. Proquest website. Accessed April 8, 2024. https://www.proquest.com/docview/1437933010?pq-origsite=gscholar&fromopenview=true&sourcetype=Scholarly%20Journals&imgSeq=1  
  • McLeod S. Research hypothesis in psychology: Types & examples. SimplyPsychology website. Updated December 13, 2023. Accessed April 9, 2024. https://www.simplypsychology.org/what-is-a-hypotheses.html  
  • Scientific method. Britannica website. Updated March 14, 2024. Accessed April 9, 2024. https://www.britannica.com/science/scientific-method  
  • The hypothesis in science writing. Accessed April 10, 2024. https://berks.psu.edu/sites/berks/files/campus/HypothesisHandout_Final.pdf  
  • How to develop a hypothesis (with elements, types, and examples). Indeed.com website. Updated February 3, 2023. Accessed April 10, 2024. https://www.indeed.com/career-advice/career-development/how-to-write-a-hypothesis  
  • Types of research hypotheses. Excelsior online writing lab. Accessed April 11, 2024. https://owl.excelsior.edu/research/research-hypotheses/types-of-research-hypotheses/  
  • What is a research hypothesis: how to write it, types, and examples. Researcher.life website. Published February 8, 2023. Accessed April 11, 2024. https://researcher.life/blog/article/how-to-write-a-research-hypothesis-definition-types-examples/  
  • Developing a hypothesis. Pressbooks website. Accessed April 12, 2024. https://opentext.wsu.edu/carriecuttler/chapter/developing-a-hypothesis/  
  • What is and how to write a good hypothesis in research. Elsevier author services website. Accessed April 12, 2024. https://scientific-publishing.webshop.elsevier.com/manuscript-preparation/what-how-write-good-hypothesis-research/  
  • How to write a great hypothesis. Verywellmind website. Updated March 12, 2023. Accessed April 13, 2024. https://www.verywellmind.com/what-is-a-hypothesis-2795239  
  • 15 Hypothesis examples. Helpfulprofessor.com Published September 8, 2023. Accessed March 14, 2024. https://helpfulprofessor.com/hypothesis-examples/ 
  • Editage insights. What is the interconnectivity between research objectives and hypothesis? Published February 24, 2021. Accessed April 13, 2024. https://www.editage.com/insights/what-is-the-interconnectivity-between-research-objectives-and-hypothesis  
  • Understanding null hypothesis testing. BCCampus open publishing. Accessed April 16, 2024. https://opentextbc.ca/researchmethods/chapter/understanding-null-hypothesis-testing/#:~:text=In%20null%20hypothesis%20testing%2C%20this,said%20to%20be%20statistically%20significant  

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1.3: Scientific Theories

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As you view Know the Difference (Between Hypothesis and Theory) , focus on these concepts: the controversy surrounding the words ‘‘hypothesis’’ and ‘‘theory’’, the scientific use of the words ‘‘hypothesis’’ and ‘‘theory’’, the criteria for a ‘‘hypothesis,’’ the National Academy of Sciences definition of ‘‘theory’’, the meaning of the statement, ‘‘theories are the bedrock of our understanding of nature’’.

The Theory of Evolution

The theory of evolution by natural selection is a scientific theory. Evolution is a change in the characteristics of living things over time. Evolution occurs by a process called natural selection . In natural selection, some living things produce more offspring than others, so they pass more genes to the next generation than others do. Over many generations, this can lead to major changes in the characteristics of living things. The theory of evolution by natural selection explains how living things are changing today and how modern living things have descended from ancient life forms that no longer exist on Earth. No evidence has been identified that proves this theory is incorrect. More on the theory of evolution will be presented in additional concepts.

The Cell Theory

The cell theory is another important scientific theory of biology. According to the cell theory , the cell is the smallest unit of structure and function of all living organisms, all living organisms are made up of at least one cell, and living cells always come from other living cells. Once again, no evidence has been identified that proves this theory is incorrect. More on the cell theory will be presented in additional concepts.

The Germ Theory

The germ theory of disease, also called the pathogenic theory of medicine, is a scientific theory that proposes that microorganisms are the cause of many diseases. Like the other scientific theories, lots of evidence has been identified that supports this theory, and no evidence has been identified that proves the theory is incorrect.

  • With repeated testing, some hypotheses may eventually become scientific theories. A scientific theory is a broad explanation for events that is widely accepted as true.
  • Evolution is a change species over time. Evolution occurs by natural selection.
  • The cell theory states that all living things are made up of cells, and living cells always come from other living cells.
  • The germ theory proposes that microorganisms are the cause of many diseases.

Explore More

Use these resources to answer the questions that follow.

Explore More I

  • Darwinian Evolution - Science and Theory at Non-Majors Biology: http://www.hippocampus.org/Biology .
  • How is the word ‘‘theory’’ used in common language?
  • How is the word ‘‘theory’’ used in science?
  • Provide a detailed definition for a ‘‘scientific theory’’.

Explore More II

  • Concepts and Methods in Biology - Theories and Laws at Non-Majors Biology: http://www.hippocampus.org/Biology .
  • What is a scientific law ?
  • What is a scientific theory?
  • Give two examples of scientific theories.
  • Can a scientific theory become a law? Why or why not?
  • Contrast how the term theory is used in science and in everyday language.
  • Explain how a hypothesis could become a theory.
  • Describe the evidence that proves the cell theory is incorrect.

IMAGES

  1. Hypothesis

    hypothesis definition biology examples

  2. 13 Different Types of Hypothesis (2024)

    hypothesis definition biology examples

  3. Null hypothesis

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  4. Null hypothesis

    hypothesis definition biology examples

  5. Scientific hypothesis

    hypothesis definition biology examples

  6. What is a Hypothesis?

    hypothesis definition biology examples

VIDEO

  1. Concept of Hypothesis

  2. Hypothesis explain biology book

  3. What Is A Hypothesis?

  4. Proportion Hypothesis Testing, example 2

  5. Hypothesis Definition

  6. What does hypothesis mean?

COMMENTS

  1. Hypothesis

    Biology definition: A hypothesis is a supposition or tentative explanation for (a group of) phenomena, (a set of) facts, or a scientific inquiry that may be tested, verified or answered by further investigation or methodological experiment. It is like a scientific guess. It's an idea or prediction that scientists make before they do ...

  2. The scientific method (article)

    The scientific method. At the core of biology and other sciences lies a problem-solving approach called the scientific method. The scientific method has five basic steps, plus one feedback step: Make an observation. Ask a question. Form a hypothesis, or testable explanation. Make a prediction based on the hypothesis.

  3. Scientific hypothesis

    scientific hypothesis, an idea that proposes a tentative explanation about a phenomenon or a narrow set of phenomena observed in the natural world.The two primary features of a scientific hypothesis are falsifiability and testability, which are reflected in an "If…then" statement summarizing the idea and in the ability to be supported or refuted through observation and experimentation.

  4. Hypothesis Examples

    Here are some research hypothesis examples: If you leave the lights on, then it takes longer for people to fall asleep. If you refrigerate apples, they last longer before going bad. If you keep the curtains closed, then you need less electricity to heat or cool the house (the electric bill is lower). If you leave a bucket of water uncovered ...

  5. What is a scientific hypothesis?

    A scientific hypothesis is a tentative, testable explanation for a phenomenon in the natural world. It's the initial building block in the scientific method. Many describe it as an "educated guess ...

  6. 4.14: Experiments and Hypotheses

    Experiments and further observations are often used to test the hypotheses. A scientific experiment is a carefully organized procedure in which the scientist intervenes in a system to change something, then observes the result of the change. Scientific inquiry often involves doing experiments, though not always.

  7. Experiments and Hypotheses

    When conducting scientific experiments, researchers develop hypotheses to guide experimental design. A hypothesis is a suggested explanation that is both testable and falsifiable. You must be able to test your hypothesis through observations and research, and it must be possible to prove your hypothesis false. For example, Michael observes that ...

  8. Biology and the scientific method review

    Biology is the branch of science concerned with the study of living things, or organisms. Biologists have identified traits common to all the living organisms that we know. Although nonliving things may show some of these properties, in order for something to be considered living, it must meet all of them.

  9. 1.3: The Science of Biology

    The scientific method can be applied to almost all fields of study as a logical, rational, problem-solving method. Figure 1.3.1 1.3. 1: Sir Francis Bacon: Sir Francis Bacon (1561-1626) is credited with being the first to define the scientific method. The scientific process typically starts with an observation (often a problem to be solved ...

  10. Scientific Method

    Definition. The scientific method is a series of processes that people can use to gather knowledge about the world around them, improve that knowledge, and attempt to explain why and/or how things occur. This method involves making observations, forming questions, making hypotheses, doing an experiment, analyzing the data, and forming a conclusion.

  11. 1.1 The Science of Biology

    This is a very broad definition because the scope of biology is vast. ... For example, one hypothesis might be, "The classroom is warm because no one turned on the air conditioning." However, there could be other responses to the question, and therefore one may propose other hypotheses. A second hypothesis might be, "The classroom is warm ...

  12. What Is a Hypothesis? The Scientific Method

    A hypothesis (plural hypotheses) is a proposed explanation for an observation. The definition depends on the subject. In science, a hypothesis is part of the scientific method. It is a prediction or explanation that is tested by an experiment. Observations and experiments may disprove a scientific hypothesis, but can never entirely prove one.

  13. How to Write a Strong Hypothesis

    5. Phrase your hypothesis in three ways. To identify the variables, you can write a simple prediction in if…then form. The first part of the sentence states the independent variable and the second part states the dependent variable. If a first-year student starts attending more lectures, then their exam scores will improve.

  14. Hypothesis

    hypothesis, something supposed or taken for granted, with the object of following out its consequences (Greek hypothesis, "a putting under," the Latin equivalent being suppositio ). Discussion with Kara Rogers of how the scientific model is used to test a hypothesis or represent a theory. Kara Rogers, senior biomedical sciences editor of ...

  15. Scientific Hypothesis Examples

    Scientific Hypothesis Examples . Hypothesis: All forks have three tines. This would be disproven if you find any fork with a different number of tines. Hypothesis: There is no relationship between smoking and lung cancer.While it is difficult to establish cause and effect in health issues, you can apply statistics to data to discredit or support this hypothesis.

  16. Hypothesis: Definition, Examples, and Types

    A hypothesis is a tentative statement about the relationship between two or more variables. It is a specific, testable prediction about what you expect to happen in a study. It is a preliminary answer to your question that helps guide the research process. Consider a study designed to examine the relationship between sleep deprivation and test ...

  17. Scientific Hypothesis

    The word hypothesis basically means a prediction about what will happen. It is an educated guess and the framework of an experiment. Once you think of one, you can design an experiment to test it. Remember, you cannot prove a hypothesis is true. You can only see if the data supports your claim or not.

  18. What is a Hypothesis

    Definition: Hypothesis is an educated guess or proposed explanation for a phenomenon, based on some initial observations or data. It is a tentative statement that can be tested and potentially proven or disproven through further investigation and experimentation. Hypothesis is often used in scientific research to guide the design of experiments ...

  19. 1.2: The Science of Biology

    Descriptive science and hypothesis-based science are in continuous dialogue. Figure \(\PageIndex{1}\): A Burr: This fruit attaches to animal fur via the hooks on its surface to improve distribution. Velcro is an example of a biomimetic invention which has copied burrs and uses small flexible hooks to reversibly attach to fluffy surfaces.

  20. Biology Hypothesis

    Follow this step-by-step guide to create a strong biology hypothesis: 1. Identify the Phenomenon: Clearly define the biological phenomenon you intend to study. This could be a question, a pattern, an observation, or a problem in the field of biology. 2.

  21. 3.14: Experiments and Hypotheses

    Experiments and further observations are often used to test the hypotheses. A scientific experiment is a carefully organized procedure in which the scientist intervenes in a system to change something, then observes the result of the change. Scientific inquiry often involves doing experiments, though not always.

  22. How to Write a Hypothesis? Types and Examples

    For example, Louis Pasteur observed that food lasts longer at higher altitudes, reasoned that it could be because the air at higher altitudes is cleaner (with fewer or no germs), and tested the hypothesis by exposing food to air cleaned in the laboratory. 12 Thus, a hypothesis is predictive—if the reasoning is correct, X will lead to Y—and ...

  23. 1.3: Scientific Theories

    Scientific Theories. With repeated testing, some hypotheses may eventually become scientific theories. Keep in mind, a hypothesis is a possible answer to a scientific question. A scientific theory is a broad explanation for events that is widely accepted as true. To become a theory, a hypothesis must be tested over and over again, and it must be supported by a great deal of evidence.