example of null and alternative hypothesis in biology

Null hypothesis

Null hypothesis n., plural: null hypotheses [nʌl haɪˈpɒθɪsɪs] Definition: a hypothesis that is valid or presumed true until invalidated by a statistical test

Table of Contents

Null Hypothesis Definition

Null hypothesis is defined as “the commonly accepted fact (such as the sky is blue) and researcher aim to reject or nullify this fact”.

More formally, we can define a null hypothesis as “a statistical theory suggesting that no statistical relationship exists between given observed variables” .

In biology , the null hypothesis is used to nullify or reject a common belief. The researcher carries out the research which is aimed at rejecting the commonly accepted belief.

What Is a Null Hypothesis?

A hypothesis is defined as a theory or an assumption that is based on inadequate evidence. It needs and requires more experiments and testing for confirmation. There are two possibilities that by doing more experiments and testing, a hypothesis can be false or true. It means it can either prove wrong or true (Blackwelder, 1982).

For example, Susie assumes that mineral water helps in the better growth and nourishment of plants over distilled water. To prove this hypothesis, she performs this experiment for almost a month. She watered some plants with mineral water and some with distilled water.

In a hypothesis when there are no statistically significant relationships among the two variables, the hypothesis is said to be a null hypothesis. The investigator is trying to disprove such a hypothesis. In the above example of plants, the null hypothesis is:

There are no statistical relationships among the forms of water that are given to plants for growth and nourishment.

Usually, an investigator tries to prove the null hypothesis wrong and tries to explain a relation and association between the two variables.

An opposite and reverse of the null hypothesis are known as the alternate hypothesis . In the example of plants the alternate hypothesis is:

There are statistical relationships among the forms of water that are given to plants for growth and nourishment.

The example below shows the difference between null vs alternative hypotheses:

Alternate Hypothesis: The world is round Null Hypothesis: The world is not round.

Copernicus and many other scientists try to prove the null hypothesis wrong and false. By their experiments and testing, they make people believe that alternate hypotheses are correct and true. If they do not prove the null hypothesis experimentally wrong then people will not believe them and never consider the alternative hypothesis true and correct.

The alternative and null hypothesis for Susie’s assumption is:

Null Hypothesis: If one plant is watered with distilled water and the other with mineral water, then there is no difference in the growth and nourishment of these two plants.
Alternative Hypothesis: If one plant is watered with distilled water and the other with mineral water, then the plant with mineral water shows better growth and nourishment.

The null hypothesis suggests that there is no significant or statistical relationship. The relation can either be in a single set of variables or among two sets of variables.

Most people consider the null hypothesis true and correct. Scientists work and perform different experiments and do a variety of research so that they can prove the null hypothesis wrong or nullify it. For this purpose, they design an alternate hypothesis that they think is correct or true. The null hypothesis symbol is H 0 (it is read as H null or H zero ).

Why is it named the “Null”?

The name null is given to this hypothesis to clarify and explain that the scientists are working to prove it false i.e. to nullify the hypothesis. Sometimes it confuses the readers; they might misunderstand it and think that statement has nothing. It is blank but, actually, it is not. It is more appropriate and suitable to call it a nullifiable hypothesis instead of the null hypothesis.

Why do we need to assess it? Why not just verify an alternate one?

In science, the scientific method is used. It involves a series of different steps. Scientists perform these steps so that a hypothesis can be proved false or true. Scientists do this to confirm that there will be any limitation or inadequacy in the new hypothesis. Experiments are done by considering both alternative and null hypotheses, which makes the research safe. It gives a negative as well as a bad impact on research if a null hypothesis is not included or a part of the study. It seems like you are not taking your research seriously and not concerned about it and just want to impose your results as correct and true if the null hypothesis is not a part of the study.

Development of the Null

In statistics, firstly it is necessary to design alternate and null hypotheses from the given problem. Splitting the problem into small steps makes the pathway towards the solution easier and less challenging. how to write a null hypothesis?

Writing a null hypothesis consists of two steps:

Firstly, initiate by asking a question.
Secondly, restate the question in such a way that it seems there are no relationships among the variables.

In other words, assume in such a way that the treatment does not have any effect.

The usual recovery duration after knee surgery is considered almost 8 weeks.

A researcher thinks that the recovery period may get elongated if patients go to a physiotherapist for rehabilitation twice per week, instead of thrice per week, i.e. recovery duration reduces if the patient goes three times for rehabilitation instead of two times.

Step 1: Look for the problem in the hypothesis. The hypothesis either be a word or can be a statement. In the above example the hypothesis is:

“The expected recovery period in knee rehabilitation is more than 8 weeks”

Step 2: Make a mathematical statement from the hypothesis. Averages can also be represented as μ, thus the null hypothesis formula will be.

In the above equation, the hypothesis is equivalent to H1, the average is denoted by μ and > that the average is greater than eight.

Step 3: Explain what will come up if the hypothesis does not come right i.e., the rehabilitation period may not proceed more than 08 weeks.

There are two options: either the recovery will be less than or equal to 8 weeks.

H 0 : μ ≤ 8

In the above equation, the null hypothesis is equivalent to H 0 , the average is denoted by μ and ≤ represents that the average is less than or equal to eight.

What will happen if the scientist does not have any knowledge about the outcome?

Problem: An investigator investigates the post-operative impact and influence of radical exercise on patients who have operative procedures of the knee. The chances are either the exercise will improve the recovery or will make it worse. The usual time for recovery is 8 weeks.

Step 1: Make a null hypothesis i.e. the exercise does not show any effect and the recovery time remains almost 8 weeks.

H 0 : μ = 8

In the above equation, the null hypothesis is equivalent to H 0 , the average is denoted by μ, and the equal sign (=) shows that the average is equal to eight.

Step 2: Make the alternate hypothesis which is the reverse of the null hypothesis. Particularly what will happen if treatment (exercise) makes an impact?

In the above equation, the alternate hypothesis is equivalent to H1, the average is denoted by μ and not equal sign (≠) represents that the average is not equal to eight.

Significance Tests

To get a reasonable and probable clarification of statistics (data), a significance test is performed. The null hypothesis does not have data. It is a piece of information or statement which contains numerical figures about the population. The data can be in different forms like in means or proportions. It can either be the difference of proportions and means or any odd ratio.

The following table will explain the symbols:

P-value is the chief statistical final result of the significance test of the null hypothesis.

P-value = Pr(data or data more extreme | H 0 true)
| = “given”
Pr = probability
H 0 = the null hypothesis

The first stage of Null Hypothesis Significance Testing (NHST) is to form an alternate and null hypothesis. By this, the research question can be briefly explained.

Null Hypothesis = no effect of treatment, no difference, no association Alternative Hypothesis = effective treatment, difference, association

When to reject the null hypothesis?

Researchers will reject the null hypothesis if it is proven wrong after experimentation. Researchers accept null hypothesis to be true and correct until it is proven wrong or false. On the other hand, the researchers try to strengthen the alternate hypothesis. The binomial test is performed on a sample and after that, a series of tests were performed (Frick, 1995).

Step 1: Evaluate and read the research question carefully and consciously and make a null hypothesis. Verify the sample that supports the binomial proportion. If there is no difference then find out the value of the binomial parameter.

Show the null hypothesis as:

H 0 :p= the value of p if H 0 is true

To find out how much it varies from the proposed data and the value of the null hypothesis, calculate the sample proportion.

Step 2: In test statistics, find the binomial test that comes under the null hypothesis. The test must be based on precise and thorough probabilities. Also make a list of pmf that apply, when the null hypothesis proves true and correct.

When H 0 is true, X~b(n, p)

N = size of the sample

P = assume value if H 0 proves true.

Step 3: Find out the value of P. P-value is the probability of data that is under observation.

Rise or increase in the P value = Pr(X ≥ x)

X = observed number of successes

P value = Pr(X ≤ x).

Step 4: Demonstrate the findings or outcomes in a descriptive detailed way.

Sample proportion
The direction of difference (either increases or decreases)

Perceived Problems With the Null Hypothesis

Variable or model selection and less information in some cases are the chief important issues that affect the testing of the null hypothesis. Statistical tests of the null hypothesis are reasonably not strong. There is randomization about significance. (Gill, 1999) The main issue with the testing of the null hypothesis is that they all are wrong or false on a ground basis.

There is another problem with the a-level . This is an ignored but also a well-known problem. The value of a-level is without a theoretical basis and thus there is randomization in conventional values, most commonly 0.q, 0.5, or 0.01. If a fixed value of a is used, it will result in the formation of two categories (significant and non-significant) The issue of a randomized rejection or non-rejection is also present when there is a practical matter which is the strong point of the evidence related to a scientific matter.

The P-value has the foremost importance in the testing of null hypothesis but as an inferential tool and for interpretation, it has a problem. The P-value is the probability of getting a test statistic at least as extreme as the observed one.

The main point about the definition is: Observed results are not based on a-value

Moreover, the evidence against the null hypothesis was overstated due to unobserved results. A-value has importance more than just being a statement. It is a precise statement about the evidence from the observed results or data. Similarly, researchers found that P-values are objectionable. They do not prefer null hypotheses in testing. It is also clear that the P-value is strictly dependent on the null hypothesis. It is computer-based statistics. In some precise experiments, the null hypothesis statistics and actual sampling distribution are closely related but this does not become possible in observational studies.

Some researchers pointed out that the P-value is depending on the sample size. If the true and exact difference is small, a null hypothesis even of a large sample may get rejected. This shows the difference between biological importance and statistical significance. (Killeen, 2005)

Another issue is the fix a-level, i.e., 0.1. On the basis, if a-level a null hypothesis of a large sample may get accepted or rejected. If the size of simple is infinity and the null hypothesis is proved true there are still chances of Type I error. That is the reason this approach or method is not considered consistent and reliable. There is also another problem that the exact information about the precision and size of the estimated effect cannot be known. The only solution is to state the size of the effect and its precision.

Null Hypothesis Examples

Here are some examples:

Example 1: Hypotheses with One Sample of One Categorical Variable

Among all the population of humans, almost 10% of people prefer to do their task with their left hand i.e. left-handed. Let suppose, a researcher in the Penn States says that the population of students at the College of Arts and Architecture is mostly left-handed as compared to the general population of humans in general public society. In this case, there is only a sample and there is a comparison among the known population values to the population proportion of sample value.

Research Question: Do artists more expected to be left-handed as compared to the common population persons in society?
Response Variable: Sorting the student into two categories. One category has left-handed persons and the other category have right-handed persons.
Form Null Hypothesis: Arts and Architecture college students are no more predicted to be lefty as compared to the common population persons in society (Lefty students of Arts and Architecture college population is 10% or p= 0.10)

Example 2: Hypotheses with One Sample of One Measurement Variable

A generic brand of antihistamine Diphenhydramine making medicine in the form of a capsule, having a 50mg dose. The maker of the medicines is concerned that the machine has come out of calibration and is not making more capsules with the suitable and appropriate dose.

Research Question: Does the statistical data recommended about the mean and average dosage of the population differ from 50mg?
Response Variable: Chemical assay used to find the appropriate dosage of the active ingredient.
Null Hypothesis: Usually, the 50mg dosage of capsules of this trade name (population average and means dosage =50 mg).

Example 3: Hypotheses with Two Samples of One Categorical Variable

Several people choose vegetarian meals on a daily basis. Typically, the researcher thought that females like vegetarian meals more than males.

Research Question: Does the data recommend that females (women) prefer vegetarian meals more than males (men) regularly?
Response Variable: Cataloguing the persons into vegetarian and non-vegetarian categories. Grouping Variable: Gender
Null Hypothesis: Gender is not linked to those who like vegetarian meals. (Population percent of women who eat vegetarian meals regularly = population percent of men who eat vegetarian meals regularly or p women = p men).

Example 4: Hypotheses with Two Samples of One Measurement Variable

Nowadays obesity and being overweight is one of the major and dangerous health issues. Research is performed to confirm that a low carbohydrates diet leads to faster weight loss than a low-fat diet.

Research Question: Does the given data recommend that usually, a low-carbohydrate diet helps in losing weight faster as compared to a low-fat diet?
Response Variable: Weight loss (pounds)
Explanatory Variable: Form of diet either low carbohydrate or low fat
Null Hypothesis: There is no significant difference when comparing the mean loss of weight of people using a low carbohydrate diet to people using a diet having low fat. (population means loss of weight on a low carbohydrate diet = population means loss of weight on a diet containing low fat).

Example 5: Hypotheses about the relationship between Two Categorical Variables

A case-control study was performed. The study contains nonsmokers, stroke patients, and controls. The subjects are of the same occupation and age and the question was asked if someone at their home or close surrounding smokes?

Research Question: Did second-hand smoke enhance the chances of stroke?
Variables: There are 02 diverse categories of variables. (Controls and stroke patients) (whether the smoker lives in the same house). The chances of having a stroke will be increased if a person is living with a smoker.
Null Hypothesis: There is no significant relationship between a passive smoker and stroke or brain attack. (odds ratio between stroke and the passive smoker is equal to 1).

Example 6: Hypotheses about the relationship between Two Measurement Variables

A financial expert observes that there is somehow a positive and effective relationship between the variation in stock rate price and the quantity of stock bought by non-management employees

Response variable- Regular alteration in price
Explanatory Variable- Stock bought by non-management employees
Null Hypothesis: The association and relationship between the regular stock price alteration ($) and the daily stock-buying by non-management employees ($) = 0.

Example 7: Hypotheses about comparing the relationship between Two Measurement Variables in Two Samples

Research Question: Is the relation between the bill paid in a restaurant and the tip given to the waiter, is linear? Is this relation different for dining and family restaurants?
Explanatory Variable- total bill amount
Response Variable- the amount of tip
Null Hypothesis: The relationship and association between the total bill quantity at a family or dining restaurant and the tip, is the same.

Try to answer the quiz below to check what you have learned so far about the null hypothesis.

Choose the best answer.

Send Your Results (Optional)

Blackwelder, W. C. (1982). “Proving the null hypothesis” in clinical trials. Controlled Clinical Trials , 3(4), 345–353.
Frick, R. W. (1995). Accepting the null hypothesis. Memory & Cognition, 23(1), 132–138.
Gill, J. (1999). The insignificance of null hypothesis significance testing. Political Research Quarterly , 52(3), 647–674.
Killeen, P. R. (2005). An alternative to null-hypothesis significance tests. Psychological Science, 16(5), 345–353.

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Last updated on June 16th, 2022

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Null and Alternative Hypotheses | Definitions & Examples

Null & Alternative Hypotheses | Definitions, Templates & Examples

Published on May 6, 2022 by Shaun Turney . Revised on June 22, 2023.

The null and alternative hypotheses are two competing claims that researchers weigh evidence for and against using a statistical test :

Null hypothesis ( H 0 ): There’s no effect in the population .
Alternative hypothesis ( H a or H 1 ) : There’s an effect in the population.

Answering your research question with hypotheses, what is a null hypothesis, what is an alternative hypothesis, similarities and differences between null and alternative hypotheses, how to write null and alternative hypotheses, other interesting articles, frequently asked questions.

The null and alternative hypotheses offer competing answers to your research question . When the research question asks “Does the independent variable affect the dependent variable?”:

The null hypothesis ( H 0 ) answers “No, there’s no effect in the population.”
The alternative hypothesis ( H a ) answers “Yes, there is an effect in the population.”

The null and alternative are always claims about the population. That’s because the goal of hypothesis testing is to make inferences about a population based on a sample . Often, we infer whether there’s an effect in the population by looking at differences between groups or relationships between variables in the sample. It’s critical for your research to write strong hypotheses .

You can use a statistical test to decide whether the evidence favors the null or alternative hypothesis. Each type of statistical test comes with a specific way of phrasing the null and alternative hypothesis. However, the hypotheses can also be phrased in a general way that applies to any test.

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The null hypothesis is the claim that there’s no effect in the population.

If the sample provides enough evidence against the claim that there’s no effect in the population ( p ≤ α), then we can reject the null hypothesis . Otherwise, we fail to reject the null hypothesis.

Although “fail to reject” may sound awkward, it’s the only wording that statisticians accept . Be careful not to say you “prove” or “accept” the null hypothesis.

Null hypotheses often include phrases such as “no effect,” “no difference,” or “no relationship.” When written in mathematical terms, they always include an equality (usually =, but sometimes ≥ or ≤).

You can never know with complete certainty whether there is an effect in the population. Some percentage of the time, your inference about the population will be incorrect. When you incorrectly reject the null hypothesis, it’s called a type I error . When you incorrectly fail to reject it, it’s a type II error.

Examples of null hypotheses

The table below gives examples of research questions and null hypotheses. There’s always more than one way to answer a research question, but these null hypotheses can help you get started.

*Note that some researchers prefer to always write the null hypothesis in terms of “no effect” and “=”. It would be fine to say that daily meditation has no effect on the incidence of depression and p 1 = p 2 .

The alternative hypothesis ( H a ) is the other answer to your research question . It claims that there’s an effect in the population.

Often, your alternative hypothesis is the same as your research hypothesis. In other words, it’s the claim that you expect or hope will be true.

The alternative hypothesis is the complement to the null hypothesis. Null and alternative hypotheses are exhaustive, meaning that together they cover every possible outcome. They are also mutually exclusive, meaning that only one can be true at a time.

Alternative hypotheses often include phrases such as “an effect,” “a difference,” or “a relationship.” When alternative hypotheses are written in mathematical terms, they always include an inequality (usually ≠, but sometimes < or >). As with null hypotheses, there are many acceptable ways to phrase an alternative hypothesis.

Examples of alternative hypotheses

The table below gives examples of research questions and alternative hypotheses to help you get started with formulating your own.

Null and alternative hypotheses are similar in some ways:

They’re both answers to the research question.
They both make claims about the population.
They’re both evaluated by statistical tests.

However, there are important differences between the two types of hypotheses, summarized in the following table.

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To help you write your hypotheses, you can use the template sentences below. If you know which statistical test you’re going to use, you can use the test-specific template sentences. Otherwise, you can use the general template sentences.

General template sentences

The only thing you need to know to use these general template sentences are your dependent and independent variables. To write your research question, null hypothesis, and alternative hypothesis, fill in the following sentences with your variables:

Does independent variable affect dependent variable ?

Null hypothesis ( H 0 ): Independent variable does not affect dependent variable.
Alternative hypothesis ( H a ): Independent variable affects dependent variable.

Test-specific template sentences

Once you know the statistical test you’ll be using, you can write your hypotheses in a more precise and mathematical way specific to the test you chose. The table below provides template sentences for common statistical tests.

Note: The template sentences above assume that you’re performing one-tailed tests . One-tailed tests are appropriate for most studies.

If you want to know more about statistics , methodology , or research bias , make sure to check out some of our other articles with explanations and examples.

Normal distribution
Descriptive statistics
Measures of central tendency
Correlation coefficient

Methodology

Cluster sampling
Stratified sampling
Types of interviews
Cohort study
Thematic analysis

Research bias

Implicit bias
Cognitive bias
Survivorship bias
Availability heuristic
Nonresponse bias
Regression to the mean

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

Null and alternative hypotheses are used in statistical hypothesis testing . The null hypothesis of a test always predicts no effect or no relationship between variables, while the alternative hypothesis states your research prediction of an effect or relationship.

The null hypothesis is often abbreviated as H 0 . When the null hypothesis is written using mathematical symbols, it always includes an equality symbol (usually =, but sometimes ≥ or ≤).

The alternative hypothesis is often abbreviated as H a or H 1 . When the alternative hypothesis is written using mathematical symbols, it always includes an inequality symbol (usually ≠, but sometimes < or >).

A research hypothesis is your proposed answer to your research question. The research hypothesis usually includes an explanation (“ x affects y because …”).

A statistical hypothesis, on the other hand, is a mathematical statement about a population parameter. Statistical hypotheses always come in pairs: the null and alternative hypotheses . In a well-designed study , the statistical hypotheses correspond logically to the research hypothesis.

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Null Hypothesis Examples

The null hypothesis (H 0 ) is the hypothesis that states there is no statistical difference between two sample sets. In other words, it assumes the independent variable does not have an effect on the dependent variable in a scientific experiment .

The null hypothesis is the most powerful type of hypothesis in the scientific method because it’s the easiest one to test with a high confidence level using statistics. If the null hypothesis is accepted, then it’s evidence any observed differences between two experiment groups are due to random chance. If the null hypothesis is rejected, then it’s strong evidence there is a true difference between test sets or that the independent variable affects the dependent variable.

The null hypothesis is a nullifiable hypothesis. A researcher seeks to reject it because this result strongly indicates observed differences are real and not just due to chance.
The null hypothesis may be accepted or rejected, but not proven. There is always a level of confidence in the outcome.

What Is the Null Hypothesis?

The null hypothesis is written as H 0 , which is read as H-zero, H-nought, or H-null. It is associated with another hypothesis, called the alternate or alternative hypothesis H A or H 1 . When the null hypothesis and alternate hypothesis are written mathematically, they cover all possible outcomes of an experiment.

An experimenter tests the null hypothesis with a statistical analysis called a significance test. The significance test determines the likelihood that the results of the test are not due to chance. Usually, a researcher uses a confidence level of 95% or 99% (p-value of 0.05 or 0.01). But, even if the confidence in the test is high, there is always a small chance the outcome is incorrect. This means you can’t prove a null hypothesis. It’s also a good reason why it’s important to repeat experiments.

Exact and Inexact Null Hypothesis

The most common type of null hypothesis assumes no difference between two samples or groups or no measurable effect of a treatment. This is the exact hypothesis . If you’re asked to state a null hypothesis for a science class, this is the one to write. It is the easiest type of hypothesis to test and is the only one accepted for certain types of analysis. Examples include:

There is no difference between two groups H 0 : μ 1 = μ 2 (where H 0 = the null hypothesis, μ 1 = the mean of population 1, and μ 2 = the mean of population 2)

Both groups have value of 100 (or any number or quality) H 0 : μ = 100

However, sometimes a researcher may test an inexact hypothesis . This type of hypothesis specifies ranges or intervals. Examples include:

Recovery time from a treatment is the same or worse than a placebo: H 0 : μ ≥ placebo time

There is a 5% or less difference between two groups: H 0 : 95 ≤ μ ≤ 105

An inexact hypothesis offers “directionality” about a phenomenon. For example, an exact hypothesis can indicate whether or not a treatment has an effect, while an inexact hypothesis can tell whether an effect is positive of negative. However, an inexact hypothesis may be harder to test and some scientists and statisticians disagree about whether it’s a true null hypothesis .

How to State the Null Hypothesis

To state the null hypothesis, first state what you expect the experiment to show. Then, rephrase the statement in a form that assumes there is no relationship between the variables or that a treatment has no effect.

Example: A researcher tests whether a new drug speeds recovery time from a certain disease. The average recovery time without treatment is 3 weeks.

State the goal of the experiment: “I hope the average recovery time with the new drug will be less than 3 weeks.”
Rephrase the hypothesis to assume the treatment has no effect: “If the drug doesn’t shorten recovery time, then the average time will be 3 weeks or longer.” Mathematically: H 0 : μ ≥ 3

This null hypothesis (inexact hypothesis) covers both the scenario in which the drug has no effect and the one in which the drugs makes the recovery time longer. The alternate hypothesis is that average recovery time will be less than three weeks:

H A : μ < 3

Of course, the researcher could test the no-effect hypothesis (exact null hypothesis): H 0 : μ = 3

The danger of testing this hypothesis is that rejecting it only implies the drug affected recovery time (not whether it made it better or worse). This is because the alternate hypothesis is:

H A : μ ≠ 3 (which includes μ <3 and μ >3)

Even though the no-effect null hypothesis yields less information, it’s used because it’s easier to test using statistics. Basically, testing whether something is unchanged/changed is easier than trying to quantify the nature of the change.

Remember, a researcher hopes to reject the null hypothesis because this supports the alternate hypothesis. Also, be sure the null and alternate hypothesis cover all outcomes. Finally, remember a simple true/false, equal/unequal, yes/no exact hypothesis is easier to test than a more complex inexact hypothesis.

Adèr, H. J.; Mellenbergh, G. J. & Hand, D. J. (2007). Advising on Research Methods: A Consultant’s Companion . Huizen, The Netherlands: Johannes van Kessel Publishing. ISBN 978-90-79418-01-5 .
Cox, D. R. (2006). Principles of Statistical Inference . Cambridge University Press. ISBN 978-0-521-68567-2 .
Everitt, Brian (1998). The Cambridge Dictionary of Statistics . Cambridge, UK New York: Cambridge University Press. ISBN 978-0521593465.
Weiss, Neil A. (1999). Introductory Statistics (5th ed.). ISBN 9780201598773.

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Knowledge Base
Null and Alternative Hypotheses | Definitions & Examples

Null and Alternative Hypotheses | Definitions & Examples

Published on 5 October 2022 by Shaun Turney . Revised on 6 December 2022.

The null and alternative hypotheses are two competing claims that researchers weigh evidence for and against using a statistical test :

Null hypothesis (H 0 ): There’s no effect in the population .
Alternative hypothesis (H A ): There’s an effect in the population.

The effect is usually the effect of the independent variable on the dependent variable .

Answering your research question with hypotheses, what is a null hypothesis, what is an alternative hypothesis, differences between null and alternative hypotheses, how to write null and alternative hypotheses, frequently asked questions about null and alternative hypotheses.

The null and alternative hypotheses offer competing answers to your research question . When the research question asks “Does the independent variable affect the dependent variable?”, the null hypothesis (H 0 ) answers “No, there’s no effect in the population.” On the other hand, the alternative hypothesis (H A ) answers “Yes, there is an effect in the population.”

The null hypothesis is the claim that there’s no effect in the population.

Although “fail to reject” may sound awkward, it’s the only wording that statisticians accept. Be careful not to say you “prove” or “accept” the null hypothesis.

Null hypotheses often include phrases such as “no effect”, “no difference”, or “no relationship”. When written in mathematical terms, they always include an equality (usually =, but sometimes ≥ or ≤).

Examples of null hypotheses

The table below gives examples of research questions and null hypotheses. There’s always more than one way to answer a research question, but these null hypotheses can help you get started.

The alternative hypothesis (H A ) is the other answer to your research question . It claims that there’s an effect in the population.

Often, your alternative hypothesis is the same as your research hypothesis. In other words, it’s the claim that you expect or hope will be true.

Alternative hypotheses often include phrases such as “an effect”, “a difference”, or “a relationship”. When alternative hypotheses are written in mathematical terms, they always include an inequality (usually ≠, but sometimes > or <). As with null hypotheses, there are many acceptable ways to phrase an alternative hypothesis.

Examples of alternative hypotheses

The table below gives examples of research questions and alternative hypotheses to help you get started with formulating your own.

Null and alternative hypotheses are similar in some ways:

They’re both answers to the research question
They both make claims about the population
They’re both evaluated by statistical tests.

However, there are important differences between the two types of hypotheses, summarized in the following table.

Does independent variable affect dependent variable ?

Null hypothesis (H 0 ): Independent variable does not affect dependent variable .
Alternative hypothesis (H A ): Independent variable affects dependent variable .

Test-specific

Note: The template sentences above assume that you’re performing one-tailed tests . One-tailed tests are appropriate for most studies.

The null hypothesis is often abbreviated as H 0 . When the null hypothesis is written using mathematical symbols, it always includes an equality symbol (usually =, but sometimes ≥ or ≤).

A research hypothesis is your proposed answer to your research question. The research hypothesis usually includes an explanation (‘ x affects y because …’).

A statistical hypothesis, on the other hand, is a mathematical statement about a population parameter. Statistical hypotheses always come in pairs: the null and alternative hypotheses. In a well-designed study , the statistical hypotheses correspond logically to the research hypothesis.

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8.1 – The null and alternative hypotheses

Introduction

Statistical Inference in the NHST Framework

Nhst workflow, null hypothesis, alternative hypothesis, alternative hypothesis often may be the research hypothesis, how to interpret the results of a statistical test, outcomes of an experiment, chapter 8 contents.

$X^{2}$

a calculated test statistic
degrees of freedom associated with the calculation of the test statistic
Recall from our previous discussion (Chapter 8.2) that this is not strictly the interpretation of p-value, but a short-hand for how likely the data fit the null hypothesis. P-value alone can’t tell us about “truth.”
in the event we reject the null hypothesis, we provisionally accept the alternative hypothesis .

By inference we mean to imply some formal process by which a conclusion is reached from data analysis of outcomes of an experiment. The process at its best leads to conclusions based on evidence. In statistics, evidence comes about from the careful and reasoned application of statistical procedures and the evaluation of probability (Abelson 1995).

Formally, statistics is rich in inference process. We begin by defining the classical frequentist, aka Neyman-Pearson approach, to inference, which involves the pairing of two kinds of statistical hypotheses: the null hypothesis (H O ) and the alternate hypothesis (H A ). Whether we accept the hull hypothesis or not is evaluated against a decision criterion, a fixed statistical significance level (Lehmann 1992). Significance level refers to the setting of a p-value threshold before testing is done. The threshold is often set to Type I error of 5% (Cowles & Davis 1982), but researchers should always consider whether this threshold is appropriate for their work (Benjamin et al 2017).

This inference process is referred to as Null Hypothesis Significance Testing, NHST. Additionally, a probability value will be obtained for the test outcome or test statistic value. In the Fisherian likelihood tradition, the magnitude of this statistic value can be associated with a probability value, the p-value, of how likely the result is given the null hypothesis is “true”. (Again, keep in mind that this is not strictly the interpretation of p-value, it’s a short-hand for how likely the data fit the null hypothesis. P-value alone can’t tell us about “truth”, per our discussion, Chapter 8.2 .)

About -logP . P-values are traditionally reported as a decimal, like 0.000134, in the closed (set) interval (0,1) — p-values can never be exactly zero or one. The smaller the value, the less the chance our data agree with the null prediction. Small numbers like this can be confusing, particularly if many p-values are reported, like in many genomics works, e.g., GWAS studies. Instead of reporting vanishingly small p-values, studies may report the negative log 10 p-value , or -logP . Instead of small numbers, large numbers are reported, the larger, the more against the null hypothesis. Thus, our p-value becomes 3.87 -logP.

Why log 10 and not some other base transform? Just that log 10 is convenience — powers of 10.

The antilog of 3.87 returns our p-value

For convenience, a partial p-value -logP transform table

On your own, complete the table up to -logP 5 – 10. See Question 7 below .

We presented in the introduction to Chapter 8 without discussion a simple flow chart to illustrate the process of decision (Figure 1). Here, we repeat the flow chart diagram and follow with descriptions of the elements.

Figure 1. Flow chart of inductive statistical reasoning.

What’s missing from the flow chart is the very necessary caveat that interpretation of the null hypothesis is associated with two kinds of error, Type I error and Type II error. These points and others are discussed in the following sections.

We start with the hypothesis statements. For illustration we discuss hypotheses in terms of comparisons involving just two groups, also called two sample tests . One sample tests in contrast refer to scenarios where you compare a sample statistic to a population value. Extending these concepts to more than two samples is straight-forward, but we leave that discussion to Chapters 12 – 18.

By far the most common application of the null hypothesis testing paradigm involves the comparisons of different treatment groups on some outcome variable. These kinds of null hypotheses are the subject of Chapters 8 through 12.

The Null hypothesis (H O ) is a statement about the comparisons, e.g., between a sample statistic and the population, or between two treatment groups. The former is referred to as a one tailed test whereas the latter is called a two-tailed test . The null hypothesis is typically “no statistical difference” between the comparisons.

For example, a one sample, two tailed null hypothesis.

$\begin{align*} H_{0}: \bar{X} = \mu \end{align*}$

and we read it as “there is no statistical difference between our sample mean and the population mean.” For the more likely case in which no population mean is available, we provide another example, a two sample, two tailed null hypothesis.

$\begin{align*} H_{A}: \bar{X}_{1} = \bar{X}_{2} \end{align*}$

Here, we read the statement as “there is no difference between our two sample means.” Equivalently, we interpret the statement as both sample means estimate the same population mean.

$\begin{align*} H_{A}: \bar{X}_{1} = \bar{X}_{2} = \mu \end{align*}$

Under the Neyman-Pearson approach to inference we have two hypotheses: the null hypothesis and the alternate hypothesis. The hull hypothesis was defined above.

Tails of a test are discussed further in chapter 8.4 .

Alternative hypothesis (H A ): If we conclude that the null hypothesis is false, or rather and more precisely, we find that we provisionally fail to reject the null hypothesis, then we provisionally accept the alternative hypothesis . The view then is that something other than random chance has influenced the sample observations. Note that the pairing of null and alternative hypotheses covers all possible outcomes. We do not, however, say that we have evidence for the alternative hypothesis under this statistical regimen (Abelson 1995). We tested the null hypothesis, not the alternative hypothesis. Thus, it is incorrect to write that, having found a statistical difference between two drug treatments, say aspirin and acetaminophen for relief of migraine symptoms, it is not correct to conclude that we have proven the case that acetaminophen improves improves symptoms of migraine sufferers.

For the one sample, two tailed null hypothesis, the alternative hypothesis is

$\begin{align*} H_{A}: \bar{X}\neq \mu \end{align*}$

and we read it as “there is a statistical difference between our sample mean and the population mean.” For the two sample, two tailed null hypothesis, the alternative hypothesis would be

$\begin{align*} H_{A}: \bar{X}_{1}\neq \bar{X}_{2} \end{align*}$

and we read it as “there is a statistical difference between our two sample means.”

It may be helpful to distinguish between technical hypotheses, scientific hypothesis, or the equality of different kinds of treatments. Tests of technical hypotheses include the testing of statistical assumptions like normality assumption (see Chapter 13.3 ) and homogeneity of variances ( Chapter 13.4 ). The results of inferences about technical hypotheses are used by the statistician to justify selection of parametric statistical tests ( Chapter 13 ). The testing of some scientific hypothesis like whether or not there is a positive link between lifespan and insulin-like growth factor levels in humans (Fontana et al 2008), like the link between lifespan and IGFs in other organisms (Holtzenberger et al 2003), can be further advanced by considering multiple hypotheses and a test of nested hypotheses and evaluated either in Bayesian or likelihood approaches ( Chapter 16 and Chapter 17 ).

Any number of statistical tests may be used to calculate the value of the test statistic . For example, a one sample t-test may be used to evaluate the difference between the sample mean and the population mean ( Chapter 8.5 ) or the independent sample t-test may be used to evaluate the difference between means of the control group and the treatment group ( Chapter 10 ). The test statistic is the particular value of the outcome of our evaluation of the hypothesis and it is associated with the p-value. In other words, given the assumption of a particular probability distribution, in this case the t-distribution, we can associate a probability, the p-value, that we observed the particular value of the test statistic and the null hypothesis is true in the reference population.

By convention, we determine statistical significance (Cox 1982; Whitley & Ball 2002) by assigning ahead of time a decision probability called the Type I error rate , often given the symbol α (alpha). The practice is to look up the critical value that corresponds to the outcome of the test with degrees of freedom like your experiment and at the Type I error rate that you selected. The Degrees of Freedom (DF, df, or sometimes noted by the symbol v ), are the number of independent pieces of information available to you. Knowing the degrees of freedom is a crucial piece of information for making the correct tests. Each statistical test has a specific formula for obtaining the independent information available for the statistical test. We first were introduced to DF when we calculated the sample variance with the Bessel correction , n – 1, instead of dividing through by n. With the df in hand, the value of the test statistic is compared to the critical value for our null hypothesis. If the test statistic is smaller than the critical value, we fail to reject the null hypothesis. If, however, the test statistic is greater than the critical value, then we provisionally reject the null hypothesis. This critical value comes from a probability distribution appropriate for the kind of sampling and properties of the measurement we are using. In other words, the rejection criterion for the null hypothesis is set to a critical value, which corresponds to a known probability, the Type I error rate.

Before proceeding with yet another interpretation, and hopefully less technical discussion about test statistics and critical values, we need to discuss the two types of statistical errors. The Type I error rate is the statistical error assigned to the probability that we may reject a null hypothesis as a result of our evaluation of our data when in fact in the reference population, the null hypothesis is, in fact, true. In Biology we generally use Type I error α = 0.05 level of significance. We say that the probability of obtaining the observed value AND H O is true is 1 in 20 (5%) if α = 0.05. Put another way, we are willing to reject the Null Hypothesis when there is only a 5% chance that the observations could occur and the Null hypothesis is still true. Our test statistic is associated with the p-value; the critical value is associated with the Type I error rate. If and only if the test statistic value equals the critical value will the p-value equal the Type I error rate.

The second error type associated with hypothesis testing is, β, the Type II statistical error rate . This is the case where we accept or fail to reject a null hypothesis based on our data, but in the reference population, the situation is that indeed, the null hypothesis is actually false.

Thus, we end with a concept that may take you a while to come to terms with — there are four, not two possible outcomes of an experiment.

What are the possible outcomes of a comparative experiment\? We have two treatments, one in which subjects are given a treatment and the other, subjects receive a placebo. Subjects are followed and an outcome is measured. We calculate the descriptive statistics aka summary statistics, means, standard deviations, and perhaps other statistics, and then ask whether there is a difference between the statistics for the groups. So, two possible outcomes of the experiment, correct\? If the treatment has no effect, then we would expect the two groups to have roughly the same values for means, etc., in other words, any difference between the groups is due to chance fluctuations in the measurements and not because of any systematic effect due to the treatment received. Conversely, then if there is a difference due to the treatment, we expect to see a large enough difference in the statistics so that we would notice the systematic effect due to the treatment.

Actually, there are four, not two, possible outcomes of an experiment, just as there were four and not two conclusions about the results of a clinical assay. The four possible outcomes of a test of a statistical null hypothesis are illustrated in Table 1.

Table 1. When conducting hypothesis testing, four outcomes are possible.

In the actual population, a thing happens or it doesn’t. The null hypothesis is either true or it is not. But we don’t have access to the reference population, we don’t have a census. In other words, there is truth, but we don’t have access to the truth. We can weight, assigned as a probability or p-value, our decisions by how likely our results are given the assumption that the truth is indeed “no difference.”

If you recall, we’ve seen a table like Table 1 before in our discussion of conditional probability and risk analysis ( Chapter 7.3 ). We made the point that statistical inference and the interpretation of clinical tests are similar (Browner and Newman 1987). From the perspective of ordering a diagnostic test , the proper null hypothesis would be the patient does not have the disease. For your review, here’s that table (Table 2).

Table 2. Interpretations of results of a diagnostic or clinical test.

Thus, a positive diagnostic test result is interpreted as rejecting the null hypothesis. If the person actually does not have the disease, then the positive diagnostic test is a false positive.

Match the corresponding entries in the two tables. For example, which outcome from the inference/hypothesis table matches specificity of the test\ ?
Find three sources on the web for definitions of the p-value. Write out these definitions in your notes and compare them.
In your own words distinguish between the test statistic and the critical value.
Can the p-value associated with the test statistic ever be zero\? Explain.
Since the p-value is associated with the test statistic and the null hypothesis is true, what value must the p-value be for us to provisionally reject the null hypothesis\?
All of our discussions have been about testing the null hypothesis, about accepting or rejecting, provisionally, the null hypothesis. If we reject the null hypothesis, can we say that we have evidence for the alternate hypothesis\?
What are the p-values for -logP of 5, 6, 7, 8, 9, and 10\? Complete the p-value -logP transform table .
Instead of log 10 transform, create a similar table but for negative natural log transform. Which is more convenient? Hint: log(x, base=exp(1))
The null and alternative hypotheses
The controversy over proper hypothesis testing
Sampling distribution and hypothesis testing
Tails of a test
One sample t-test
Confidence limits for the estimate of population mean
References and suggested readings

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9.1: Null and Alternative Hypotheses

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The actual test begins by considering two hypotheses . They are called the null hypothesis and the alternative hypothesis . These hypotheses contain opposing viewpoints.

$H_0$: The null hypothesis: It is a statement of no difference between the variables—they are not related. This can often be considered the status quo and as a result if you cannot accept the null it requires some action.

$H_a$: The alternative hypothesis: It is a claim about the population that is contradictory to $H_0$ and what we conclude when we reject $H_0$. This is usually what the researcher is trying to prove.

Since the null and alternative hypotheses are contradictory, you must examine evidence to decide if you have enough evidence to reject the null hypothesis or not. The evidence is in the form of sample data.

After you have determined which hypothesis the sample supports, you make a decision. There are two options for a decision. They are "reject $H_0$" if the sample information favors the alternative hypothesis or "do not reject $H_0$" or "decline to reject $H_0$" if the sample information is insufficient to reject the null hypothesis.

$H_{0}$ always has a symbol with an equal in it. $H_{a}$ never has a symbol with an equal in it. The choice of symbol depends on the wording of the hypothesis test. However, be aware that many researchers (including one of the co-authors in research work) use = in the null hypothesis, even with > or < as the symbol in the alternative hypothesis. This practice is acceptable because we only make the decision to reject or not reject the null hypothesis.

Example $\PageIndex{1}$

$H_{0}$: No more than 30% of the registered voters in Santa Clara County voted in the primary election. $p \leq 30$
$H_{a}$: More than 30% of the registered voters in Santa Clara County voted in the primary election. $p > 30$

Exercise $\PageIndex{1}$

A medical trial is conducted to test whether or not a new medicine reduces cholesterol by 25%. State the null and alternative hypotheses.

$H_{0}$: The drug reduces cholesterol by 25%. $p = 0.25$
$H_{a}$: The drug does not reduce cholesterol by 25%. $p \neq 0.25$

Example $\PageIndex{2}$

We want to test whether the mean GPA of students in American colleges is different from 2.0 (out of 4.0). The null and alternative hypotheses are:

$H_{0}: \mu = 2.0$
$H_{a}: \mu \neq 2.0$

Exercise $\PageIndex{2}$

We want to test whether the mean height of eighth graders is 66 inches. State the null and alternative hypotheses. Fill in the correct symbol $(=, \neq, \geq, <, \leq, >)$ for the null and alternative hypotheses.

$H_{0}: \mu \_ 66$
$H_{a}: \mu \_ 66$
$H_{0}: \mu = 66$
$H_{a}: \mu \neq 66$

Example $\PageIndex{3}$

We want to test if college students take less than five years to graduate from college, on the average. The null and alternative hypotheses are:

$H_{0}: \mu \geq 5$
$H_{a}: \mu < 5$

Exercise $\PageIndex{3}$

We want to test if it takes fewer than 45 minutes to teach a lesson plan. State the null and alternative hypotheses. Fill in the correct symbol ( =, ≠, ≥, <, ≤, >) for the null and alternative hypotheses.

$H_{0}: \mu \_ 45$
$H_{a}: \mu \_ 45$
$H_{0}: \mu \geq 45$
$H_{a}: \mu < 45$

Example $\PageIndex{4}$

In an issue of U. S. News and World Report , an article on school standards stated that about half of all students in France, Germany, and Israel take advanced placement exams and a third pass. The same article stated that 6.6% of U.S. students take advanced placement exams and 4.4% pass. Test if the percentage of U.S. students who take advanced placement exams is more than 6.6%. State the null and alternative hypotheses.

$H_{0}: p \leq 0.066$
$H_{a}: p > 0.066$

Exercise $\PageIndex{4}$

On a state driver’s test, about 40% pass the test on the first try. We want to test if more than 40% pass on the first try. Fill in the correct symbol ($=, \neq, \geq, <, \leq, >$) for the null and alternative hypotheses.

$H_{0}: p \_ 0.40$
$H_{a}: p \_ 0.40$
$H_{0}: p = 0.40$
$H_{a}: p > 0.40$

COLLABORATIVE EXERCISE

Bring to class a newspaper, some news magazines, and some Internet articles . In groups, find articles from which your group can write null and alternative hypotheses. Discuss your hypotheses with the rest of the class.

In a hypothesis test , sample data is evaluated in order to arrive at a decision about some type of claim. If certain conditions about the sample are satisfied, then the claim can be evaluated for a population. In a hypothesis test, we:

Evaluate the null hypothesis , typically denoted with $H_{0}$. The null is not rejected unless the hypothesis test shows otherwise. The null statement must always contain some form of equality $(=, \leq \text{or} \geq)$
Always write the alternative hypothesis , typically denoted with $H_{a}$ or $H_{1}$, using less than, greater than, or not equals symbols, i.e., $(\neq, >, \text{or} <)$.
If we reject the null hypothesis, then we can assume there is enough evidence to support the alternative hypothesis.
Never state that a claim is proven true or false. Keep in mind the underlying fact that hypothesis testing is based on probability laws; therefore, we can talk only in terms of non-absolute certainties.

Formula Review

$H_{0}$ and $H_{a}$ are contradictory.

If $\alpha \leq p$-value, then do not reject $H_{0}$.
If$\alpha > p$-value, then reject $H_{0}$.

$\alpha$ is preconceived. Its value is set before the hypothesis test starts. The $p$-value is calculated from the data.References

Data from the National Institute of Mental Health. Available online at http://www.nimh.nih.gov/publicat/depression.cfm .

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Genetics and Statistical Analysis

Once you have performed an experiment, how can you tell if your results are significant? For example, say that you are performing a genetic cross in which you know the genotypes of the parents. In this situation, you might hypothesize that the cross will result in a certain ratio of phenotypes in the offspring . But what if your observed results do not exactly match your expectations? How can you tell whether this deviation was due to chance? The key to answering these questions is the use of statistics , which allows you to determine whether your data are consistent with your hypothesis.

Forming and Testing a Hypothesis

The first thing any scientist does before performing an experiment is to form a hypothesis about the experiment's outcome. This often takes the form of a null hypothesis , which is a statistical hypothesis that states there will be no difference between observed and expected data. The null hypothesis is proposed by a scientist before completing an experiment, and it can be either supported by data or disproved in favor of an alternate hypothesis.

Let's consider some examples of the use of the null hypothesis in a genetics experiment. Remember that Mendelian inheritance deals with traits that show discontinuous variation, which means that the phenotypes fall into distinct categories. As a consequence, in a Mendelian genetic cross, the null hypothesis is usually an extrinsic hypothesis ; in other words, the expected proportions can be predicted and calculated before the experiment starts. Then an experiment can be designed to determine whether the data confirm or reject the hypothesis. On the other hand, in another experiment, you might hypothesize that two genes are linked. This is called an intrinsic hypothesis , which is a hypothesis in which the expected proportions are calculated after the experiment is done using some information from the experimental data (McDonald, 2008).

How Math Merged with Biology

But how did mathematics and genetics come to be linked through the use of hypotheses and statistical analysis? The key figure in this process was Karl Pearson, a turn-of-the-century mathematician who was fascinated with biology. When asked what his first memory was, Pearson responded by saying, "Well, I do not know how old I was, but I was sitting in a high chair and I was sucking my thumb. Someone told me to stop sucking it and said that if I did so, the thumb would wither away. I put my two thumbs together and looked at them a long time. ‘They look alike to me,' I said to myself, ‘I can't see that the thumb I suck is any smaller than the other. I wonder if she could be lying to me'" (Walker, 1958). As this anecdote illustrates, Pearson was perhaps born to be a scientist. He was a sharp observer and intent on interpreting his own data. During his career, Pearson developed statistical theories and applied them to the exploration of biological data. His innovations were not well received, however, and he faced an arduous struggle in convincing other scientists to accept the idea that mathematics should be applied to biology. For instance, during Pearson's time, the Royal Society, which is the United Kingdom's academy of science, would accept papers that concerned either mathematics or biology, but it refused to accept papers than concerned both subjects (Walker, 1958). In response, Pearson, along with Francis Galton and W. F. R. Weldon, founded a new journal called Biometrika in 1901 to promote the statistical analysis of data on heredity. Pearson's persistence paid off. Today, statistical tests are essential for examining biological data.

Pearson's Chi-Square Test for Goodness-of-Fit

One of Pearson's most significant achievements occurred in 1900, when he developed a statistical test called Pearson's chi-square (Χ 2 ) test, also known as the chi-square test for goodness-of-fit (Pearson, 1900). Pearson's chi-square test is used to examine the role of chance in producing deviations between observed and expected values. The test depends on an extrinsic hypothesis, because it requires theoretical expected values to be calculated. The test indicates the probability that chance alone produced the deviation between the expected and the observed values (Pierce, 2005). When the probability calculated from Pearson's chi-square test is high, it is assumed that chance alone produced the difference. Conversely, when the probability is low, it is assumed that a significant factor other than chance produced the deviation.

In 1912, J. Arthur Harris applied Pearson's chi-square test to examine Mendelian ratios (Harris, 1912). It is important to note that when Gregor Mendel studied inheritance, he did not use statistics, and neither did Bateson, Saunders, Punnett, and Morgan during their experiments that discovered genetic linkage . Thus, until Pearson's statistical tests were applied to biological data, scientists judged the goodness of fit between theoretical and observed experimental results simply by inspecting the data and drawing conclusions (Harris, 1912). Although this method can work perfectly if one's data exactly matches one's predictions, scientific experiments often have variability associated with them, and this makes statistical tests very useful.

The chi-square value is calculated using the following formula:

Using this formula, the difference between the observed and expected frequencies is calculated for each experimental outcome category. The difference is then squared and divided by the expected frequency . Finally, the chi-square values for each outcome are summed together, as represented by the summation sign (Σ).

Pearson's chi-square test works well with genetic data as long as there are enough expected values in each group. In the case of small samples (less than 10 in any category) that have 1 degree of freedom, the test is not reliable. (Degrees of freedom, or df, will be explained in full later in this article.) However, in such cases, the test can be corrected by using the Yates correction for continuity, which reduces the absolute value of each difference between observed and expected frequencies by 0.5 before squaring. Additionally, it is important to remember that the chi-square test can only be applied to numbers of progeny , not to proportions or percentages.

Now that you know the rules for using the test, it's time to consider an example of how to calculate Pearson's chi-square. Recall that when Mendel crossed his pea plants, he learned that tall (T) was dominant to short (t). You want to confirm that this is correct, so you start by formulating the following null hypothesis: In a cross between two heterozygote (Tt) plants, the offspring should occur in a 3:1 ratio of tall plants to short plants. Next, you cross the plants, and after the cross, you measure the characteristics of 400 offspring. You note that there are 305 tall pea plants and 95 short pea plants; these are your observed values. Meanwhile, you expect that there will be 300 tall plants and 100 short plants from the Mendelian ratio.

You are now ready to perform statistical analysis of your results, but first, you have to choose a critical value at which to reject your null hypothesis. You opt for a critical value probability of 0.01 (1%) that the deviation between the observed and expected values is due to chance. This means that if the probability is less than 0.01, then the deviation is significant and not due to chance, and you will reject your null hypothesis. However, if the deviation is greater than 0.01, then the deviation is not significant and you will not reject the null hypothesis.

So, should you reject your null hypothesis or not? Here's a summary of your observed and expected data:

Now, let's calculate Pearson's chi-square:

For tall plants: Χ 2 = (305 - 300) 2 / 300 = 0.08
For short plants: Χ 2 = (95 - 100) 2 / 100 = 0.25
The sum of the two categories is 0.08 + 0.25 = 0.33
Therefore, the overall Pearson's chi-square for the experiment is Χ 2 = 0.33

Next, you determine the probability that is associated with your calculated chi-square value. To do this, you compare your calculated chi-square value with theoretical values in a chi-square table that has the same number of degrees of freedom. Degrees of freedom represent the number of ways in which the observed outcome categories are free to vary. For Pearson's chi-square test, the degrees of freedom are equal to n - 1, where n represents the number of different expected phenotypes (Pierce, 2005). In your experiment, there are two expected outcome phenotypes (tall and short), so n = 2 categories, and the degrees of freedom equal 2 - 1 = 1. Thus, with your calculated chi-square value (0.33) and the associated degrees of freedom (1), you can determine the probability by using a chi-square table (Table 1).

Table 1: Chi-Square Table

(Table adapted from Jones, 2008)

Note that the chi-square table is organized with degrees of freedom (df) in the left column and probabilities (P) at the top. The chi-square values associated with the probabilities are in the center of the table. To determine the probability, first locate the row for the degrees of freedom for your experiment, then determine where the calculated chi-square value would be placed among the theoretical values in the corresponding row.

At the beginning of your experiment, you decided that if the probability was less than 0.01, you would reject your null hypothesis because the deviation would be significant and not due to chance. Now, looking at the row that corresponds to 1 degree of freedom, you see that your calculated chi-square value of 0.33 falls between 0.016, which is associated with a probability of 0.9, and 2.706, which is associated with a probability of 0.10. Therefore, there is between a 10% and 90% probability that the deviation you observed between your expected and the observed numbers of tall and short plants is due to chance. In other words, the probability associated with your chi-square value is much greater than the critical value of 0.01. This means that we will not reject our null hypothesis, and the deviation between the observed and expected results is not significant.

Level of Significance

Determining whether to accept or reject a hypothesis is decided by the experimenter, who is the person who chooses the "level of significance" or confidence. Scientists commonly use the 0.05, 0.01, or 0.001 probability levels as cut-off values. For instance, in the example experiment, you used the 0.01 probability. Thus, P ≥ 0.01 can be interpreted to mean that chance likely caused the deviation between the observed and the expected values (i.e. there is a greater than 1% probability that chance explains the data). If instead we had observed that P ≤ 0.01, this would mean that there is less than a 1% probability that our data can be explained by chance. There is a significant difference between our expected and observed results, so the deviation must be caused by something other than chance.

References and Recommended Reading

Harris, J. A. A simple test of the goodness of fit of Mendelian ratios. American Naturalist 46 , 741–745 (1912)

Jones, J. "Table: Chi-Square Probabilities." http://people.richland.edu/james/lecture/m170/tbl-chi.html (2008) (accessed July 7, 2008)

McDonald, J. H. Chi-square test for goodness-of-fit. From The Handbook of Biological Statistics . http://udel.edu/~mcdonald/statchigof.html (2008) (accessed June 9, 2008)

Pearson, K. On the criterion that a given system of deviations from the probable in the case of correlated system of variables is such that it can be reasonably supposed to have arisen from random sampling. Philosophical Magazine 50 , 157–175 (1900)

Pierce, B. Genetics: A Conceptual Approach (New York, Freeman, 2005)

Walker, H. M. The contributions of Karl Pearson. Journal of the American Statistical Association 53 , 11–22 (1958)

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9.1 Null and Alternative Hypotheses

The actual test begins by considering two hypotheses . They are called the null hypothesis and the alternative hypothesis . These hypotheses contain opposing viewpoints.

H 0 , the — null hypothesis: a statement of no difference between sample means or proportions or no difference between a sample mean or proportion and a population mean or proportion. In other words, the difference equals 0.

H a —, the alternative hypothesis: a claim about the population that is contradictory to H 0 and what we conclude when we reject H 0 .

After you have determined which hypothesis the sample supports, you make a decision. There are two options for a decision. They are reject H 0 if the sample information favors the alternative hypothesis or do not reject H 0 or decline to reject H 0 if the sample information is insufficient to reject the null hypothesis.

Mathematical Symbols Used in H 0 and H a :

H 0 always has a symbol with an equal in it. H a never has a symbol with an equal in it. The choice of symbol depends on the wording of the hypothesis test. However, be aware that many researchers use = in the null hypothesis, even with > or < as the symbol in the alternative hypothesis. This practice is acceptable because we only make the decision to reject or not reject the null hypothesis.

Example 9.1

H 0 : No more than 30 percent of the registered voters in Santa Clara County voted in the primary election. p ≤ 30 H a : More than 30 percent of the registered voters in Santa Clara County voted in the primary election. p > 30

A medical trial is conducted to test whether or not a new medicine reduces cholesterol by 25 percent. State the null and alternative hypotheses.

Example 9.2

We want to test whether the mean GPA of students in American colleges is different from 2.0 (out of 4.0). The null and alternative hypotheses are the following: H 0 : μ = 2.0 H a : μ ≠ 2.0

We want to test whether the mean height of eighth graders is 66 inches. State the null and alternative hypotheses. Fill in the correct symbol (=, ≠, ≥, <, ≤, >) for the null and alternative hypotheses.

H 0 : μ __ 66
H a : μ __ 66

Example 9.3

We want to test if college students take fewer than five years to graduate from college, on the average. The null and alternative hypotheses are the following: H 0 : μ ≥ 5 H a : μ < 5

H 0 : μ __ 45
H a : μ __ 45

Example 9.4

An article on school standards stated that about half of all students in France, Germany, and Israel take advanced placement exams and a third of the students pass. The same article stated that 6.6 percent of U.S. students take advanced placement exams and 4.4 percent pass. Test if the percentage of U.S. students who take advanced placement exams is more than 6.6 percent. State the null and alternative hypotheses. H 0 : p ≤ 0.066 H a : p > 0.066

On a state driver’s test, about 40 percent pass the test on the first try. We want to test if more than 40 percent pass on the first try. Fill in the correct symbol (=, ≠, ≥, <, ≤, >) for the null and alternative hypotheses.

H 0 : p __ 0.40
H a : p __ 0.40

Collaborative Exercise

Bring to class a newspaper, some news magazines, and some internet articles. In groups, find articles from which your group can write null and alternative hypotheses. Discuss your hypotheses with the rest of the class.

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2.2: Standard Statistical Hypothesis Testing

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Luke J. Harmon
University of Idaho

Standard hypothesis testing approaches focus almost entirely on rejecting null hypotheses. In the framework (usually referred to as the frequentist approach to statistics) one first defines a null hypothesis. This null hypothesis represents your expectation if some pattern, such as a difference among groups, is not present, or if some process of interest were not occurring. For example, perhaps you are interested in comparing the mean body size of two species of lizards, an anole and a gecko. Our null hypothesis would be that the two species do not differ in body size. The alternative, which one can conclude by rejecting that null hypothesis, is that one species is larger than the other. Another example might involve investigating two variables, like body size and leg length, across a set of lizard species 1 . Here the null hypothesis would be that there is no relationship between body size and leg length. The alternative hypothesis, which again represents the situation where the phenomenon of interest is actually occurring, is that there is a relationship with body size and leg length. For frequentist approaches, the alternative hypothesis is always the negation of the null hypothesis; as you will see below, other approaches allow one to compare the fit of a set of models without this restriction and choose the best amongst them.

The next step is to define a test statistic, some way of measuring the patterns in the data. In the two examples above, we would consider test statistics that measure the difference in mean body size among our two species of lizards, or the slope of the relationship between body size and leg length, respectively. One can then compare the value of this test statistic in the data to the expectation of this test statistic under the null hypothesis. The relationship between the test statistic and its expectation under the null hypothesis is captured by a P-value. The P-value is the probability of obtaining a test statistic at least as extreme as the actual test statistic in the case where the null hypothesis is true. You can think of the P-value as a measure of how probable it is that you would obtain your data in a universe where the null hypothesis is true. In other words, the P-value measures how probable it is under the null hypothesis that you would obtain a test statistic at least as extreme as what you see in the data. In particular, if the P-value is very large, say P = 0.94, then it is extremely likely that your data are compatible with this null hypothesis.

If the test statistic is very different from what one would expect under the null hypothesis, then the P-value will be small. This means that we are unlikely to obtain the test statistic seen in the data if the null hypothesis were true. In that case, we reject the null hypothesis as long as P is less than some value chosen in advance. This value is the significance threshold, α , and is almost always set to α = 0.05. By contrast, if that probability is large, then there is nothing “special” about your data, at least from the standpoint of your null hypothesis. The test statistic is within the range expected under the null hypothesis, and we fail to reject that null hypothesis. Note the careful language here – in a standard frequentist framework, you never accept the null hypothesis, you simply fail to reject it.

Getting back to our lizard-flipping example, we can use a frequentist approach. In this case, our particular example has a name; this is a binomial test, which assesses whether a given event with two outcomes has a certain probability of success. In this case, we are interested in testing the null hypothesis that our lizard is a fair flipper; that is, that the probability of heads p H = 0.5. The binomial test uses the number of “successes” (we will use the number of heads, H = 63) as a test statistic. We then ask whether this test statistic is either much larger or much smaller than we might expect under our null hypothesis. So, our null hypothesis is that p H = 0.5; our alternative, then, is that p H takes some other value: p H ≠ 0.5.

To carry out the test, we first need to consider how many "successes" we should expect if the null hypothesis were true. We consider the distribution of our test statistic (the number of heads) under our null hypothesis ( p H = 0.5). This distribution is a binomial distribution (Figure 2.1).

Figure 2.1. The unfair lizard. We use the null hypothesis to generate a null distribution for our test statistic, which in this case is a binomial distribution centered around 50. We then look at our test statistic and calculate the probability of obtaining a result at least as extreme as this value. Image by the author, can be reused under a CC-BY-4.0 license.

We can use the known probabilities of the binomial distribution to calculate our P-value. We want to know the probability of obtaining a result at least as extreme as our data when drawing from a binomial distribution with parameters p = 0.5 and n = 100. We calculate the area of this distribution that lies to the right of 63. This area, P = 0.003, can be obtained either from a table, from statistical software, or by using a relatively simple calculation. The value, 0.003, represents the probability of obtaining at least 63 heads out of 100 trials with p H = 0.5. This number is the P-value from our binomial test. Because we only calculated the area of our null distribution in one tail (in this case, the right, where values are greater than or equal to 63), then this is actually a one-tailed test, and we are only considering part of our null hypothesis where p H > 0.5. Such an approach might be suitable in some cases, but more typically we need to multiply this number by 2 to get a two-tailed test; thus, P = 0.006. This two-tailed P-value of 0.006 includes the possibility of results as extreme as our test statistic in either direction, either too many or too few heads. Since P < 0.05, our chosen α value, we reject the null hypothesis, and conclude that we have an unfair lizard.

In biology, null hypotheses play a critical role in many statistical analyses. So why not end this chapter now? One issue is that biological null hypotheses are almost always uninteresting. They often describe the situation where patterns in the data occur only by chance. However, if you are comparing living species to each other, there are almost always some differences between them. In fact, for biology, null hypotheses are quite often obviously false. For example, two different species living in different habitats are not identical, and if we measure them enough we will discover this fact. From this point of view, both outcomes of a standard hypothesis test are unenlightening. One either rejects a silly hypothesis that was probably known to be false from the start, or one “fails to reject” this null hypothesis 2 . There is much more information to be gained by estimating parameter values and carrying out model selection in a likelihood or Bayesian framework, as we will see below. Still, frequentist statistical approaches are common, have their place in our toolbox, and will come up in several sections of this book.

One key concept in standard hypothesis testing is the idea of statistical error. Statistical errors come in two flavors: type I and type II errors. Type I errors occur when the null hypothesis is true but the investigator mistakenly rejects it. Standard hypothesis testing controls type I errors using a parameter, α , which defines the accepted rate of type I errors. For example, if α = 0.05, one should expect to commit a type I error about 5% of the time. When multiple standard hypothesis tests are carried out, investigators often “correct” their P-values using Bonferroni correction. If you do this, then there is only a 5% chance of a single type I error across all of the tests being considered. This singular focus on type I errors, however, has a cost. One can also commit type II errors, when the null hypothesis is false but one fails to reject it. The rate of type II errors in statistical tests can be extremely high. While statisticians do take care to create approaches that have high power, traditional hypothesis testing usually fixes type I errors at 5% while type II error rates remain unknown. There are simple ways to calculate type II error rates (e.g. power analyses) but these are only rarely carried out. Furthermore, Bonferroni correction dramatically increases the type II error rate. This is important because – as stated by Perneger (1998) – “… type II errors are no less false than type I errors.” This extreme emphasis on controlling type I errors at the expense of type II errors is, to me, the main weakness of the frequentist approach 3 .

I will cover some examples of the frequentist approach in this book, mainly when discussing traditional methods like phylogenetic independent contrasts (PICs). Also, one of the model selection approaches used frequently in this book, likelihood ratio tests, rely on a standard frequentist set-up with null and alternative hypotheses.

However, there are two good reasons to look for better ways to do comparative statistics. First, as stated above, standard methods rely on testing null hypotheses that – for evolutionary questions - are usually very likely, a priori, to be false. For a relevant example, consider a study comparing the rate of speciation between two clades of carnivores. The null hypothesis is that the two clades have exactly equal rates of speciation – which is almost certainly false, although we might question how different the two rates might be. Second, in my opinion, standard frequentist methods place too much emphasis on P-values and not enough on the size of statistical effects. A small P-value could reflect either a large effect or very large sample sizes or both.

In summary, frequentist statistical methods are common in comparative statistics but can be limiting. I will discuss these methods often in this book, mainly due to their prevalent use in the field. At the same time, we will look for alternatives whenever possible.

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10.1 - setting the hypotheses: examples.

A significance test examines whether the null hypothesis provides a plausible explanation of the data. The null hypothesis itself does not involve the data. It is a statement about a parameter (a numerical characteristic of the population). These population values might be proportions or means or differences between means or proportions or correlations or odds ratios or any other numerical summary of the population. The alternative hypothesis is typically the research hypothesis of interest. Here are some examples.

Example 10.2: Hypotheses with One Sample of One Categorical Variable Section

About 10% of the human population is left-handed. Suppose a researcher at Penn State speculates that students in the College of Arts and Architecture are more likely to be left-handed than people found in the general population. We only have one sample since we will be comparing a population proportion based on a sample value to a known population value.

Research Question : Are artists more likely to be left-handed than people found in the general population?
Response Variable : Classification of the student as either right-handed or left-handed

State Null and Alternative Hypotheses

Null Hypothesis : Students in the College of Arts and Architecture are no more likely to be left-handed than people in the general population (population percent of left-handed students in the College of Art and Architecture = 10% or p = .10).
Alternative Hypothesis : Students in the College of Arts and Architecture are more likely to be left-handed than people in the general population (population percent of left-handed students in the College of Arts and Architecture > 10% or p > .10). This is a one-sided alternative hypothesis.

Example 10.3: Hypotheses with One Sample of One Measurement Variable Section

A generic brand of the anti-histamine Diphenhydramine markets a capsule with a 50 milligram dose. The manufacturer is worried that the machine that fills the capsules has come out of calibration and is no longer creating capsules with the appropriate dosage.

Research Question : Does the data suggest that the population mean dosage of this brand is different than 50 mg?
Response Variable : dosage of the active ingredient found by a chemical assay.
Null Hypothesis : On the average, the dosage sold under this brand is 50 mg (population mean dosage = 50 mg).
Alternative Hypothesis : On the average, the dosage sold under this brand is not 50 mg (population mean dosage ≠ 50 mg). This is a two-sided alternative hypothesis.

Example 10.4: Hypotheses with Two Samples of One Categorical Variable Section

Many people are starting to prefer vegetarian meals on a regular basis. Specifically, a researcher believes that females are more likely than males to eat vegetarian meals on a regular basis.

Research Question : Does the data suggest that females are more likely than males to eat vegetarian meals on a regular basis?
Response Variable : Classification of whether or not a person eats vegetarian meals on a regular basis
Explanatory (Grouping) Variable: Sex
Null Hypothesis : There is no sex effect regarding those who eat vegetarian meals on a regular basis (population percent of females who eat vegetarian meals on a regular basis = population percent of males who eat vegetarian meals on a regular basis or p females = p males ).
Alternative Hypothesis : Females are more likely than males to eat vegetarian meals on a regular basis (population percent of females who eat vegetarian meals on a regular basis > population percent of males who eat vegetarian meals on a regular basis or p females > p males ). This is a one-sided alternative hypothesis.

Example 10.5: Hypotheses with Two Samples of One Measurement Variable Section

Obesity is a major health problem today. Research is starting to show that people may be able to lose more weight on a low carbohydrate diet than on a low fat diet.

Research Question : Does the data suggest that, on the average, people are able to lose more weight on a low carbohydrate diet than on a low fat diet?
Response Variable : Weight loss (pounds)
Explanatory (Grouping) Variable : Type of diet
Null Hypothesis : There is no difference in the mean amount of weight loss when comparing a low carbohydrate diet with a low fat diet (population mean weight loss on a low carbohydrate diet = population mean weight loss on a low fat diet).
Alternative Hypothesis : The mean weight loss should be greater for those on a low carbohydrate diet when compared with those on a low fat diet (population mean weight loss on a low carbohydrate diet > population mean weight loss on a low fat diet). This is a one-sided alternative hypothesis.

Example 10.6: Hypotheses about the relationship between Two Categorical Variables Section

Research Question : Do the odds of having a stroke increase if you inhale second hand smoke ? A case-control study of non-smoking stroke patients and controls of the same age and occupation are asked if someone in their household smokes.
Variables : There are two different categorical variables (Stroke patient vs control and whether the subject lives in the same household as a smoker). Living with a smoker (or not) is the natural explanatory variable and having a stroke (or not) is the natural response variable in this situation.
Null Hypothesis : There is no relationship between whether or not a person has a stroke and whether or not a person lives with a smoker (odds ratio between stroke and second-hand smoke situation is = 1).
Alternative Hypothesis : There is a relationship between whether or not a person has a stroke and whether or not a person lives with a smoker (odds ratio between stroke and second-hand smoke situation is > 1). This is a one-tailed alternative.

This research question might also be addressed like example 11.4 by making the hypotheses about comparing the proportion of stroke patients that live with smokers to the proportion of controls that live with smokers.

Example 10.7: Hypotheses about the relationship between Two Measurement Variables Section

Research Question : A financial analyst believes there might be a positive association between the change in a stock's price and the amount of the stock purchased by non-management employees the previous day (stock trading by management being under "insider-trading" regulatory restrictions).
Variables : Daily price change information (the response variable) and previous day stock purchases by non-management employees (explanatory variable). These are two different measurement variables.
Null Hypothesis : The correlation between the daily stock price change (\$) and the daily stock purchases by non-management employees (\$) = 0.
Alternative Hypothesis : The correlation between the daily stock price change (\$) and the daily stock purchases by non-management employees (\$) > 0. This is a one-sided alternative hypothesis.

Example 10.8: Hypotheses about comparing the relationship between Two Measurement Variables in Two Samples Section

Calculation of a person's approximate tip for their meal

Research Question : Is there a linear relationship between the amount of the bill (\$) at a restaurant and the tip (\$) that was left. Is the strength of this association different for family restaurants than for fine dining restaurants?
Variables : There are two different measurement variables. The size of the tip would depend on the size of the bill so the amount of the bill would be the explanatory variable and the size of the tip would be the response variable.
Null Hypothesis : The correlation between the amount of the bill (\$) at a restaurant and the tip (\$) that was left is the same at family restaurants as it is at fine dining restaurants.
Alternative Hypothesis : The correlation between the amount of the bill (\$) at a restaurant and the tip (\$) that was left is the difference at family restaurants then it is at fine dining restaurants. This is a two-sided alternative hypothesis.

Statistics Made Easy

How to Write a Null Hypothesis (5 Examples)

A hypothesis test uses sample data to determine whether or not some claim about a population parameter is true.

Whenever we perform a hypothesis test, we always write a null hypothesis and an alternative hypothesis, which take the following forms:

H 0 (Null Hypothesis): Population parameter =, ≤, ≥ some value

H A (Alternative Hypothesis): Population parameter <, >, ≠ some value

Note that the null hypothesis always contains the equal sign .

We interpret the hypotheses as follows:

Null hypothesis: The sample data provides no evidence to support some claim being made by an individual.

Alternative hypothesis: The sample data does provide sufficient evidence to support the claim being made by an individual.

For example, suppose it’s assumed that the average height of a certain species of plant is 20 inches tall. However, one botanist claims the true average height is greater than 20 inches.

To test this claim, she may go out and collect a random sample of plants. She can then use this sample data to perform a hypothesis test using the following two hypotheses:

H 0 : μ ≤ 20 (the true mean height of plants is equal to or even less than 20 inches)

H A : μ > 20 (the true mean height of plants is greater than 20 inches)

If the sample data gathered by the botanist shows that the mean height of this species of plants is significantly greater than 20 inches, she can reject the null hypothesis and conclude that the mean height is greater than 20 inches.

Read through the following examples to gain a better understanding of how to write a null hypothesis in different situations.

Example 1: Weight of Turtles

A biologist wants to test whether or not the true mean weight of a certain species of turtles is 300 pounds. To test this, he goes out and measures the weight of a random sample of 40 turtles.

Here is how to write the null and alternative hypotheses for this scenario:

H 0 : μ = 300 (the true mean weight is equal to 300 pounds)

H A : μ ≠ 300 (the true mean weight is not equal to 300 pounds)

Example 2: Height of Males

It’s assumed that the mean height of males in a certain city is 68 inches. However, an independent researcher believes the true mean height is greater than 68 inches. To test this, he goes out and collects the height of 50 males in the city.

H 0 : μ ≤ 68 (the true mean height is equal to or even less than 68 inches)

H A : μ > 68 (the true mean height is greater than 68 inches)

Example 3: Graduation Rates

A university states that 80% of all students graduate on time. However, an independent researcher believes that less than 80% of all students graduate on time. To test this, she collects data on the proportion of students who graduated on time last year at the university.

H 0 : p ≥ 0.80 (the true proportion of students who graduate on time is 80% or higher)

H A : μ < 0.80 (the true proportion of students who graduate on time is less than 80%)

Example 4: Burger Weights

A food researcher wants to test whether or not the true mean weight of a burger at a certain restaurant is 7 ounces. To test this, he goes out and measures the weight of a random sample of 20 burgers from this restaurant.

H 0 : μ = 7 (the true mean weight is equal to 7 ounces)

H A : μ ≠ 7 (the true mean weight is not equal to 7 ounces)

Example 5: Citizen Support

A politician claims that less than 30% of citizens in a certain town support a certain law. To test this, he goes out and surveys 200 citizens on whether or not they support the law.

H 0 : p ≥ .30 (the true proportion of citizens who support the law is greater than or equal to 30%)

H A : μ < 0.30 (the true proportion of citizens who support the law is less than 30%)

Additional Resources

Introduction to Hypothesis Testing Introduction to Confidence Intervals An Explanation of P-Values and Statistical Significance

Published by Zach

AP®︎/College Statistics

Course: ap®︎/college statistics > unit 10.

Idea behind hypothesis testing

Examples of null and alternative hypotheses

Writing null and alternative hypotheses
P-values and significance tests
Comparing P-values to different significance levels
Estimating a P-value from a simulation
Estimating P-values from simulations
Using P-values to make conclusions

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Video transcript

Module 9: Hypothesis Testing With One Sample

Summary: null and alternative hypotheses, key concepts.

The null hypothesis is a statement of no change or status quo.
Symbols used for the null hypothesis are =, ≤ and ≥.
The alternative hypothesis is sometimes referred to as the research hypothesis; it is what the researcher believes to be true based on the data to be collected.
Symbols used for the alternative hypothesis are ≠, > and <.

Hypothesis: a statement about the value of a population parameter. In the case of two hypotheses, the statement assumed to be true is called the null hypothesis (notation [latex]H_0[/latex]) and the contradictory statement is called the alternative hypothesis (notation [latex]H_a[/latex]).

Hypothesis Testing: Based on sample evidence, a procedure for determining whether the hypothesis stated is a reasonable statement and should not be rejected or is unreasonable and should be rejected.

Provided by : Lumen Learning. License : CC BY: Attribution
Introductory Statistics. Authored by : Barbara Illowsky, Susan Dean. Provided by : OpenStax. Located at : https://openstax.org/books/introductory-statistics/pages/9-key-terms . License : CC BY: Attribution . License Terms : Access for free at https://openstax.org/books/introductory-statistics/pages/1-introduction

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Null vs. Alternative Hypothesis

04.28.2023 • 5 min read

Sarah Thomas

Subject Matter Expert

Learn about a null versus alternative hypothesis and what they show with examples for each. Also go over the main differences and similarities between them.

In This Article

What Is a Null Hypothesis?

What is an alternative hypothesis, outcomes of a hypothesis test.

Main Differences Between Null & Alternative Hypothesis

Similarities Between Null & Alternative Hypothesis

Hypothesis Testing & Errors

In statistics, you’ll draw insights or “inferences” about population parameters using data from a sample. This process is called inferential statistics.

To make statistical inferences, you need to determine if you have enough evidence to support a certain hypothesis about the population. This is where null and alternative hypotheses come into play!

In this article, we’ll explain the differences between these two types of hypotheses, and we’ll explain the role they play in hypothesis testing.

Imagine you want to know what percent of Americans are vegetarians. You find a Gallup poll claiming ‌5% of the population was vegetarian in 2018, but your intuition tells you vegetarianism is on the rise and that ‌far more than 5% of Americans are vegetarian today.

To investigate further, you collect your own sample data by surveying 1,000 randomly selected Americans. You’ll use this random sample to determine whether it’s likely ‌the true population proportion of vegetarians is, in fact, 5% (as the Gallup data suggests) or whether it could be the case that the percentage of vegetarians is now higher.

Notice ‌that your investigation involves two rival hypotheses about the population. One hypothesis is that the proportion of vegetarians is 5%. The other hypothesis is that the proportion of vegetarians is greater than 5%. In statistics, we would call the first hypothesis the null hypothesis, and the second hypothesis the alternative hypothesis. The null hypothesis ( H 0 H_0 H 0 ) represents the status quo or what is assumed to be true about the population at the start of your investigation.

Null Hypothesis

In hypothesis testing, the null hypothesis ( H 0 H_0 H 0 ) is the default hypothesis.

It's what the status quo assumes to be true about the population.

The alternative hypothesis ( H a H_a H a or H 1 H_1 H 1 ) is the hypothesis that stands contrary to the null hypothesis. The alternative hypothesis ‌represents the research hypothesis—what you as the statistician are trying to prove with your data .

In medical studies, where scientists are trying to demonstrate whether a treatment has a significant effect on patient outcomes, the alternative hypothesis represents the hypothesis that the treatment does have an effect, while the null hypothesis represents the assumption that the treatment has no effect.

Alternative Hypothesis

The alternative hypothesis ( H a H_a H a or H 1 H_1 H 1 ) is the hypothesis being proposed in opposition to the null hypothesis.

Examples of Null and Alternative Hypotheses

In a hypothesis test, the null and alternative hypotheses must be mutually exclusive statements, meaning both hypotheses cannot be true at the same time. For example, if the null hypothesis includes an equal sign, the alternative hypothesis must state that the values being mentioned are “not equal” in some way.

Your hypotheses will also depend on the formulation of your test—are you running a one-sample T-test, a two-sample T-test, F-test for ANOVA , or a Chi-squared test? It also matters whether you are conducting a directional one-tailed test or a nondirectional two-tailed test.

Example 1: Two-Tailed T-test

Null Hypothesis: The population mean is equal to some number, x. 𝝁 = x

Alternative Hypothesis: The population mean is not equal to x. 𝝁 ≠ x

Example 2: One-tailed T-test (Right-Tailed)

Null Hypothesis: The population mean is less than or equal to some number, x. 𝝁 ≤ x Alternative Hypothesis: The population mean is greater than x. 𝝁 > x

Example 3: One-tailed T-test (Left-Tailed)

Null Hypothesis: The population mean is greater than or equal to some number, x. 𝝁 ≥ x

Alternative Hypothesis: The population mean is less than x. 𝝁 < x

By the end of a hypothesis test, you will have reached one of two conclusions.

You will run into either 2 outcomes:

Fail to reject the null hypothesis on the grounds that there's insufficient evidence to move away from the null hypothesis

Reject the null hypothesis in favor of the alternative.

Chart going over 2 possible outcomes of a hypothesis test

If you’re ‌confused about the outcomes of a hypothesis test, a good analogy is a jury trial. In a jury trial, the defendant is innocent until proven guilty. To reach a verdict of guilt, the jury must find strong evidence (beyond a reasonable doubt) that the defendant committed the crime.

This is analogous to a statistician who must assume the null hypothesis is true unless they can uncover strong evidence ( a p-value less than or equal to the significance level) in support of the alternative hypothesis.

Notice also, that a jury never concludes a defendant is innocent—only that the defendant is guilty or not guilty. This is similar to how we never conclude that the null hypothesis is true. In a hypothesis test, we never conclude ‌that the null hypothesis is true. We can only “reject” the null hypothesis or “fail to reject” it.

In this video, let’s look at the jury example again, the reasoning behind hypothesis testing, and how to form a test. It starts by stating your null and alternative hypotheses.

Main Differences Between Null and Alternative Hypothesis

Here is a summary of the key differences between the null and the alternative hypothesis test.

The null hypothesis represents the status quo; the alternative hypothesis represents an alternative statement about the population.

The null and the alternative are mutually exclusive statements, meaning both statements cannot be true at the same time.

In a medical study, the null hypothesis represents the assumption that a treatment has no statistically significant effect on the outcome being studied. The alternative hypothesis represents the belief that the treatment does have an effect.

The null hypothesis is denoted by H_0 ; the alternative hypothesis is denoted by H_a H_1

You “fail to reject” the null hypothesis when the p-value is larger than the significance level. You “reject” the null hypothesis in favor of the alternative hypothesis when the p-value is less than or equal to your test’s significance level.

Similarities Between Null and Alternative Hypothesis

The similarities between the null and alternative hypotheses are as follows.

Both the null and the alternative are statements about the same underlying data.

Both statements provide a possible answer to a statistician’s research question.

The same hypothesis test will provide evidence for or against the null and alternative hypotheses.

Hypothesis Testing and Errors

Always remember that statistical inference provides you with inferences based on probability rather than hard truths. Anytime you conduct a hypothesis test, there is a chance that you’ll reach the wrong conclusion about your data.

In statistics, we categorize these wrong conclusions into two types of errors:

Type I Errors

Type II Errors

Type I Error (ɑ)

A Type I error occurs when you reject the null hypothesis when, in fact, the null hypothesis is true. This is sometimes called a false positive and is analogous to a jury that falsely convicts an innocent defendant. The probability of making this type of error is represented by alpha, ɑ.

Type II Error (ꞵ)

A Type II error occurs when you fail to reject the null hypothesis when, in fact, the null hypothesis is false. This is sometimes called a false negative and is analogous to a jury that reaches a verdict of “not guilty,” when, in fact, the defendant has committed the crime. The probability of making this type of error is represented by beta, ꞵ.

Outcomes of a Hypothesis test showing type I and type II errors

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Null Hypothesis Examples

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The null hypothesis —which assumes that there is no meaningful relationship between two variables—may be the most valuable hypothesis for the scientific method because it is the easiest to test using a statistical analysis. This means you can support your hypothesis with a high level of confidence. Testing the null hypothesis can tell you whether your results are due to the effect of manipulating the dependent variable or due to chance.

What Is the Null Hypothesis?

The null hypothesis states there is no relationship between the measured phenomenon (the dependent variable) and the independent variable . You do not need to believe that the null hypothesis is true to test it. On the contrary, you will likely suspect that there is a relationship between a set of variables. One way to prove that this is the case is to reject the null hypothesis. Rejecting a hypothesis does not mean an experiment was "bad" or that it didn't produce results. In fact, it is often one of the first steps toward further inquiry.

To distinguish it from other hypotheses, the null hypothesis is written as H 0 (which is read as “H-nought,” "H-null," or "H-zero"). A significance test is used to determine the likelihood that the results supporting the null hypothesis are not due to chance. A confidence level of 95 percent or 99 percent is common. Keep in mind, even if the confidence level is high, there is still a small chance the null hypothesis is not true, perhaps because the experimenter did not account for a critical factor or because of chance. This is one reason why it's important to repeat experiments.

Examples of the Null Hypothesis

To write a null hypothesis, first start by asking a question. Rephrase that question in a form that assumes no relationship between the variables. In other words, assume a treatment has no effect. Write your hypothesis in a way that reflects this.

What Is a Hypothesis? (Science)
What 'Fail to Reject' Means in a Hypothesis Test
What Are the Elements of a Good Hypothesis?
Scientific Method Vocabulary Terms
Null Hypothesis Definition and Examples
Definition of a Hypothesis
Six Steps of the Scientific Method
Understanding Simple vs Controlled Experiments
Hypothesis Test for the Difference of Two Population Proportions
What Is the Difference Between Alpha and P-Values?
Null Hypothesis and Alternative Hypothesis
What Are Examples of a Hypothesis?
Hypothesis Test Example
How to Conduct a Hypothesis Test
What Is a P-Value?
The Difference Between Type I and Type II Errors in Hypothesis Testing

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Testing of Hypothesis,Null, alternative hypothesis, type-I & -II Error etc @VATAMBEDUSRAVANKUMAR
Null & Alternative Hypothesis |Statistical Hypothesis #hypothesis #samplingdistribution #statistics
Null hypothesis (H0) and Alternative hypothesis (H1)#SHORT
Null Hypothesis vs Alternate Hypothesis

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Null hypothesis
Biology definition: A null hypothesis is an assumption or proposition where an observed difference between two samples of a statistical population is purely accidental and not due to systematic causes. It is the hypothesis to be investigated through statistical hypothesis testing so that when refuted indicates that the alternative hypothesis is true. . Thus, a null hypothesis is a hypothesis ...
Null & Alternative Hypotheses
The null hypothesis (H 0) answers "No, there's no effect in the population." The alternative hypothesis (H a) answers "Yes, there is an effect in the population." The null and alternative are always claims about the population. That's because the goal of hypothesis testing is to make inferences about a population based on a sample.
Null Hypothesis Examples
An example of the null hypothesis is that light color has no effect on plant growth. The null hypothesis (H 0) is the hypothesis that states there is no statistical difference between two sample sets. In other words, it assumes the independent variable does not have an effect on the dependent variable in a scientific experiment.
1.4: Basic Concepts of Hypothesis Testing
Biological vs. Statistical Null Hypotheses. It is important to distinguish between biological null and alternative hypotheses and statistical null and alternative hypotheses. "Sexual selection by females has caused male chickens to evolve bigger feet than females" is a biological alternative hypothesis; it says something about biological processes, in this case sexual selection.
Hypothesis testing
Summary. One of the main goals of statistical hypothesis testing is to estimate the P value, which is the probability of obtaining the observed results, or something more extreme, if the null hypothesis were true. If the observed results are unlikely under the null hypothesis, your reject the null hypothesis. Alternatives to this "frequentist ...
Null Hypothesis Definition and Examples
Null Hypothesis Examples. "Hyperactivity is unrelated to eating sugar " is an example of a null hypothesis. If the hypothesis is tested and found to be false, using statistics, then a connection between hyperactivity and sugar ingestion may be indicated. A significance test is the most common statistical test used to establish confidence in a ...
Null and Alternative Hypotheses
The null and alternative hypotheses are two competing claims that researchers weigh evidence for and against using a statistical test: Null hypothesis (H0): There's no effect in the population. Alternative hypothesis (HA): There's an effect in the population. The effect is usually the effect of the independent variable on the dependent ...
8.1
These kinds of null hypotheses are the subject of Chapters 8 through 12. The Null hypothesis (H O) is a statement about the comparisons, e.g., between a sample statistic and the population, or between two treatment groups. The former is referred to as a one tailed test whereas the latter is called a two-tailed test.
9.1: Null and Alternative Hypotheses
Review. In a hypothesis test, sample data is evaluated in order to arrive at a decision about some type of claim.If certain conditions about the sample are satisfied, then the claim can be evaluated for a population. In a hypothesis test, we: Evaluate the null hypothesis, typically denoted with $H_{0}$.The null is not rejected unless the hypothesis test shows otherwise.
Genetics and Statistical Analysis
The null hypothesis is proposed by a scientist before completing an experiment, and it can be either supported by data or disproved in favor of an alternate hypothesis. Let's consider some ...
9.1 Null and Alternative Hypotheses
The actual test begins by considering two hypotheses.They are called the null hypothesis and the alternative hypothesis.These hypotheses contain opposing viewpoints. H 0, the —null hypothesis: a statement of no difference between sample means or proportions or no difference between a sample mean or proportion and a population mean or proportion. In other words, the difference equals 0.
2.2: Standard Statistical Hypothesis Testing
Luke J. Harmon. University of Idaho. Standard hypothesis testing approaches focus almost entirely on rejecting null hypotheses. In the framework (usually referred to as the frequentist approach to statistics) one first defines a null hypothesis. This null hypothesis represents your expectation if some pattern, such as a difference among groups ...
Null hypothesis and alternative hypothesis with 9 differences
The null hypothesis is a general statement that states that there is no relationship between two phenomenons under consideration or that there is no association between two groups. An alternative hypothesis is a statement that describes that there is a relationship between two selected variables in a study. Symbol. It is denoted by H 0.
Null Hypothesis and Alternative Hypothesis
Alternative hypothesis " x is not equal to y .". Null hypothesis: " x is at least y .". Alternative hypothesis " x is less than y .". Null hypothesis: " x is at most y .". Alternative hypothesis " x is greater than y .". Here are the differences between the null and alternative hypotheses and how to distinguish between them.
10.1
10.1 - Setting the Hypotheses: Examples. A significance test examines whether the null hypothesis provides a plausible explanation of the data. The null hypothesis itself does not involve the data. It is a statement about a parameter (a numerical characteristic of the population). These population values might be proportions or means or ...
How to Write a Null Hypothesis (5 Examples)
H 0 (Null Hypothesis): Population parameter =, ≤, ≥ some value. H A (Alternative Hypothesis): Population parameter <, >, ≠ some value. Note that the null hypothesis always contains the equal sign. We interpret the hypotheses as follows: Null hypothesis: The sample data provides no evidence to support some claim being made by an individual.
Null and Alternative Hypotheses
The actual test begins by considering two hypotheses.They are called the null hypothesis and the alternative hypothesis.These hypotheses contain opposing viewpoints. H 0: The null hypothesis: It is a statement about the population that either is believed to be true or is used to put forth an argument unless it can be shown to be incorrect beyond a reasonable doubt.
AP Biology Exam Prep: Research Questions + Null and Alternative
In this video, I begin discussing AP Biology Science Practice 3: Questions and Methods by explaining how questions and hypotheses are formed at the beginning...
Examples of null and alternative hypotheses
It is the opposite of your research hypothesis. The alternative hypothesis--that is, the research hypothesis--is the idea, phenomenon, observation that you want to prove. If you suspect that girls take longer to get ready for school than boys, then: Alternative: girls time > boys time. Null: girls time <= boys time.
Summary: Null and Alternative Hypotheses
The null hypothesis is a statement of no change or status quo. Symbols used for the null hypothesis are =, ≤ and ≥. The alternative hypothesis is sometimes referred to as the research hypothesis; it is what the researcher believes to be true based on the data to be collected. Symbols used for the alternative hypothesis are ≠, > and <.
Null vs. Alternative Hypothesis [Overview]
The alternative hypothesis (H a H_a H a or H 1 H_1 H 1 ) is the hypothesis being proposed in opposition to the null hypothesis. Examples of Null and Alternative Hypotheses In a hypothesis test, the null and alternative hypotheses must be mutually exclusive statements, meaning both hypotheses cannot be true at the same time.
Examples of the Null Hypothesis
Null Hypothesis Examples. The null hypothesis —which assumes that there is no meaningful relationship between two variables—may be the most valuable hypothesis for the scientific method because it is the easiest to test using a statistical analysis. This means you can support your hypothesis with a high level of confidence.
22Exam 1 Part 2 Spring 2024
What is the sample size (write down a value)? (1 Point) 1370; Write the null and alternative hypotheses in words and symbols: (4 Points) Null hypothesis: the online grocery shopping rate at the latest time point is equal to 20%. Alternative hypothesis: the online grocery shopping rate at the latest time point is NOT equal to 20%.

Null Hypothesis Definition

What Is a Null Hypothesis?

Development of the Null

Significance Tests

Perceived Problems With the Null Hypothesis

Null Hypothesis Examples

Example 1: Hypotheses with One Sample of One Categorical Variable

Example 2: Hypotheses with One Sample of One Measurement Variable

Example 3: Hypotheses with Two Samples of One Categorical Variable

Example 4: Hypotheses with Two Samples of One Measurement Variable

Example 5: Hypotheses about the relationship between Two Categorical Variables

Example 6: Hypotheses about the relationship between Two Measurement Variables

Example 7: Hypotheses about comparing the relationship between Two Measurement Variables in Two Samples

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