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Hypothesis Testing | A Step-by-Step Guide with Easy Examples

Published on November 8, 2019 by Rebecca Bevans . Revised on June 22, 2023.

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics . It is most often used by scientists to test specific predictions, called hypotheses, that arise from theories.

There are 5 main steps in hypothesis testing:

  • State your research hypothesis as a null hypothesis and alternate hypothesis (H o ) and (H a  or H 1 ).
  • Collect data in a way designed to test the hypothesis.
  • Perform an appropriate statistical test .
  • Decide whether to reject or fail to reject your null hypothesis.
  • Present the findings in your results and discussion section.

Though the specific details might vary, the procedure you will use when testing a hypothesis will always follow some version of these steps.

Table of contents

Step 1: state your null and alternate hypothesis, step 2: collect data, step 3: perform a statistical test, step 4: decide whether to reject or fail to reject your null hypothesis, step 5: present your findings, other interesting articles, frequently asked questions about hypothesis testing.

After developing your initial research hypothesis (the prediction that you want to investigate), it is important to restate it as a null (H o ) and alternate (H a ) hypothesis so that you can test it mathematically.

The alternate hypothesis is usually your initial hypothesis that predicts a relationship between variables. The null hypothesis is a prediction of no relationship between the variables you are interested in.

  • H 0 : Men are, on average, not taller than women. H a : Men are, on average, taller than women.

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For a statistical test to be valid , it is important to perform sampling and collect data in a way that is designed to test your hypothesis. If your data are not representative, then you cannot make statistical inferences about the population you are interested in.

There are a variety of statistical tests available, but they are all based on the comparison of within-group variance (how spread out the data is within a category) versus between-group variance (how different the categories are from one another).

If the between-group variance is large enough that there is little or no overlap between groups, then your statistical test will reflect that by showing a low p -value . This means it is unlikely that the differences between these groups came about by chance.

Alternatively, if there is high within-group variance and low between-group variance, then your statistical test will reflect that with a high p -value. This means it is likely that any difference you measure between groups is due to chance.

Your choice of statistical test will be based on the type of variables and the level of measurement of your collected data .

  • an estimate of the difference in average height between the two groups.
  • a p -value showing how likely you are to see this difference if the null hypothesis of no difference is true.

Based on the outcome of your statistical test, you will have to decide whether to reject or fail to reject your null hypothesis.

In most cases you will use the p -value generated by your statistical test to guide your decision. And in most cases, your predetermined level of significance for rejecting the null hypothesis will be 0.05 – that is, when there is a less than 5% chance that you would see these results if the null hypothesis were true.

In some cases, researchers choose a more conservative level of significance, such as 0.01 (1%). This minimizes the risk of incorrectly rejecting the null hypothesis ( Type I error ).

The results of hypothesis testing will be presented in the results and discussion sections of your research paper , dissertation or thesis .

In the results section you should give a brief summary of the data and a summary of the results of your statistical test (for example, the estimated difference between group means and associated p -value). In the discussion , you can discuss whether your initial hypothesis was supported by your results or not.

In the formal language of hypothesis testing, we talk about rejecting or failing to reject the null hypothesis. You will probably be asked to do this in your statistics assignments.

However, when presenting research results in academic papers we rarely talk this way. Instead, we go back to our alternate hypothesis (in this case, the hypothesis that men are on average taller than women) and state whether the result of our test did or did not support the alternate hypothesis.

If your null hypothesis was rejected, this result is interpreted as “supported the alternate hypothesis.”

These are superficial differences; you can see that they mean the same thing.

You might notice that we don’t say that we reject or fail to reject the alternate hypothesis . This is because hypothesis testing is not designed to prove or disprove anything. It is only designed to test whether a pattern we measure could have arisen spuriously, or by chance.

If we reject the null hypothesis based on our research (i.e., we find that it is unlikely that the pattern arose by chance), then we can say our test lends support to our hypothesis . But if the pattern does not pass our decision rule, meaning that it could have arisen by chance, then we say the test is inconsistent with our hypothesis .

If you want to know more about statistics , methodology , or research bias , make sure to check out some of our other articles with explanations and examples.

  • Normal distribution
  • Descriptive statistics
  • Measures of central tendency
  • Correlation coefficient

Methodology

  • Cluster sampling
  • Stratified sampling
  • Types of interviews
  • Cohort study
  • Thematic analysis

Research bias

  • Implicit bias
  • Cognitive bias
  • Survivorship bias
  • Availability heuristic
  • Nonresponse bias
  • Regression to the mean

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

A hypothesis states your predictions about what your research will find. It is a tentative answer to your research question that has not yet been tested. For some research projects, you might have to write several hypotheses that address different aspects of your research question.

A hypothesis is not just a guess — it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Null and alternative hypotheses are used in statistical hypothesis testing . The null hypothesis of a test always predicts no effect or no relationship between variables, while the alternative hypothesis states your research prediction of an effect or relationship.

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Statology

Statistics Made Easy

Introduction to Hypothesis Testing

A statistical hypothesis is an assumption about a population parameter .

For example, we may assume that the mean height of a male in the U.S. is 70 inches.

The assumption about the height is the statistical hypothesis and the true mean height of a male in the U.S. is the population parameter .

A hypothesis test is a formal statistical test we use to reject or fail to reject a statistical hypothesis.

The Two Types of Statistical Hypotheses

To test whether a statistical hypothesis about a population parameter is true, we obtain a random sample from the population and perform a hypothesis test on the sample data.

There are two types of statistical hypotheses:

The null hypothesis , denoted as H 0 , is the hypothesis that the sample data occurs purely from chance.

The alternative hypothesis , denoted as H 1 or H a , is the hypothesis that the sample data is influenced by some non-random cause.

Hypothesis Tests

A hypothesis test consists of five steps:

1. State the hypotheses. 

State the null and alternative hypotheses. These two hypotheses need to be mutually exclusive, so if one is true then the other must be false.

2. Determine a significance level to use for the hypothesis.

Decide on a significance level. Common choices are .01, .05, and .1. 

3. Find the test statistic.

Find the test statistic and the corresponding p-value. Often we are analyzing a population mean or proportion and the general formula to find the test statistic is: (sample statistic – population parameter) / (standard deviation of statistic)

4. Reject or fail to reject the null hypothesis.

Using the test statistic or the p-value, determine if you can reject or fail to reject the null hypothesis based on the significance level.

The p-value  tells us the strength of evidence in support of a null hypothesis. If the p-value is less than the significance level, we reject the null hypothesis.

5. Interpret the results. 

Interpret the results of the hypothesis test in the context of the question being asked. 

The Two Types of Decision Errors

There are two types of decision errors that one can make when doing a hypothesis test:

Type I error: You reject the null hypothesis when it is actually true. The probability of committing a Type I error is equal to the significance level, often called  alpha , and denoted as α.

Type II error: You fail to reject the null hypothesis when it is actually false. The probability of committing a Type II error is called the Power of the test or  Beta , denoted as β.

One-Tailed and Two-Tailed Tests

A statistical hypothesis can be one-tailed or two-tailed.

A one-tailed hypothesis involves making a “greater than” or “less than ” statement.

For example, suppose we assume the mean height of a male in the U.S. is greater than or equal to 70 inches. The null hypothesis would be H0: µ ≥ 70 inches and the alternative hypothesis would be Ha: µ < 70 inches.

A two-tailed hypothesis involves making an “equal to” or “not equal to” statement.

For example, suppose we assume the mean height of a male in the U.S. is equal to 70 inches. The null hypothesis would be H0: µ = 70 inches and the alternative hypothesis would be Ha: µ ≠ 70 inches.

Note: The “equal” sign is always included in the null hypothesis, whether it is =, ≥, or ≤.

Related:   What is a Directional Hypothesis?

Types of Hypothesis Tests

There are many different types of hypothesis tests you can perform depending on the type of data you’re working with and the goal of your analysis.

The following tutorials provide an explanation of the most common types of hypothesis tests:

Introduction to the One Sample t-test Introduction to the Two Sample t-test Introduction to the Paired Samples t-test Introduction to the One Proportion Z-Test Introduction to the Two Proportion Z-Test

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  • Fundamental Analysis

Hypothesis to Be Tested: Definition and 4 Steps for Testing with Example

hypothesis testing definition

What Is Hypothesis Testing?

Hypothesis testing, sometimes called significance testing, is an act in statistics whereby an analyst tests an assumption regarding a population parameter. The methodology employed by the analyst depends on the nature of the data used and the reason for the analysis.

Hypothesis testing is used to assess the plausibility of a hypothesis by using sample data. Such data may come from a larger population, or from a data-generating process. The word "population" will be used for both of these cases in the following descriptions.

Key Takeaways

  • Hypothesis testing is used to assess the plausibility of a hypothesis by using sample data.
  • The test provides evidence concerning the plausibility of the hypothesis, given the data.
  • Statistical analysts test a hypothesis by measuring and examining a random sample of the population being analyzed.
  • The four steps of hypothesis testing include stating the hypotheses, formulating an analysis plan, analyzing the sample data, and analyzing the result.

How Hypothesis Testing Works

In hypothesis testing, an  analyst  tests a statistical sample, with the goal of providing evidence on the plausibility of the null hypothesis.

Statistical analysts test a hypothesis by measuring and examining a random sample of the population being analyzed. All analysts use a random population sample to test two different hypotheses: the null hypothesis and the alternative hypothesis.

The null hypothesis is usually a hypothesis of equality between population parameters; e.g., a null hypothesis may state that the population mean return is equal to zero. The alternative hypothesis is effectively the opposite of a null hypothesis (e.g., the population mean return is not equal to zero). Thus, they are mutually exclusive , and only one can be true. However, one of the two hypotheses will always be true.

The null hypothesis is a statement about a population parameter, such as the population mean, that is assumed to be true.

4 Steps of Hypothesis Testing

All hypotheses are tested using a four-step process:

  • The first step is for the analyst to state the hypotheses.
  • The second step is to formulate an analysis plan, which outlines how the data will be evaluated.
  • The third step is to carry out the plan and analyze the sample data.
  • The final step is to analyze the results and either reject the null hypothesis, or state that the null hypothesis is plausible, given the data.

Real-World Example of Hypothesis Testing

If, for example, a person wants to test that a penny has exactly a 50% chance of landing on heads, the null hypothesis would be that 50% is correct, and the alternative hypothesis would be that 50% is not correct.

Mathematically, the null hypothesis would be represented as Ho: P = 0.5. The alternative hypothesis would be denoted as "Ha" and be identical to the null hypothesis, except with the equal sign struck-through, meaning that it does not equal 50%.

A random sample of 100 coin flips is taken, and the null hypothesis is then tested. If it is found that the 100 coin flips were distributed as 40 heads and 60 tails, the analyst would assume that a penny does not have a 50% chance of landing on heads and would reject the null hypothesis and accept the alternative hypothesis.

If, on the other hand, there were 48 heads and 52 tails, then it is plausible that the coin could be fair and still produce such a result. In cases such as this where the null hypothesis is "accepted," the analyst states that the difference between the expected results (50 heads and 50 tails) and the observed results (48 heads and 52 tails) is "explainable by chance alone."

Some staticians attribute the first hypothesis tests to satirical writer John Arbuthnot in 1710, who studied male and female births in England after observing that in nearly every year, male births exceeded female births by a slight proportion. Arbuthnot calculated that the probability of this happening by chance was small, and therefore it was due to “divine providence.”

What is Hypothesis Testing?

Hypothesis testing refers to a process used by analysts to assess the plausibility of a hypothesis by using sample data. In hypothesis testing, statisticians formulate two hypotheses: the null hypothesis and the alternative hypothesis. A null hypothesis determines there is no difference between two groups or conditions, while the alternative hypothesis determines that there is a difference. Researchers evaluate the statistical significance of the test based on the probability that the null hypothesis is true.

What are the Four Key Steps Involved in Hypothesis Testing?

Hypothesis testing begins with an analyst stating two hypotheses, with only one that can be right. The analyst then formulates an analysis plan, which outlines how the data will be evaluated. Next, they move to the testing phase and analyze the sample data. Finally, the analyst analyzes the results and either rejects the null hypothesis or states that the null hypothesis is plausible, given the data.

What are the Benefits of Hypothesis Testing?

Hypothesis testing helps assess the accuracy of new ideas or theories by testing them against data. This allows researchers to determine whether the evidence supports their hypothesis, helping to avoid false claims and conclusions. Hypothesis testing also provides a framework for decision-making based on data rather than personal opinions or biases. By relying on statistical analysis, hypothesis testing helps to reduce the effects of chance and confounding variables, providing a robust framework for making informed conclusions.

What are the Limitations of Hypothesis Testing?

Hypothesis testing relies exclusively on data and doesn’t provide a comprehensive understanding of the subject being studied. Additionally, the accuracy of the results depends on the quality of the available data and the statistical methods used. Inaccurate data or inappropriate hypothesis formulation may lead to incorrect conclusions or failed tests. Hypothesis testing can also lead to errors, such as analysts either accepting or rejecting a null hypothesis when they shouldn’t have. These errors may result in false conclusions or missed opportunities to identify significant patterns or relationships in the data.

The Bottom Line

Hypothesis testing refers to a statistical process that helps researchers and/or analysts determine the reliability of a study. By using a well-formulated hypothesis and set of statistical tests, individuals or businesses can make inferences about the population that they are studying and draw conclusions based on the data presented. There are different types of hypothesis testing, each with their own set of rules and procedures. However, all hypothesis testing methods have the same four step process, which includes stating the hypotheses, formulating an analysis plan, analyzing the sample data, and analyzing the result. Hypothesis testing plays a vital part of the scientific process, helping to test assumptions and make better data-based decisions.

Sage. " Introduction to Hypothesis Testing. " Page 4.

Elder Research. " Who Invented the Null Hypothesis? "

Formplus. " Hypothesis Testing: Definition, Uses, Limitations and Examples. "

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S.3 hypothesis testing.

In reviewing hypothesis tests, we start first with the general idea. Then, we keep returning to the basic procedures of hypothesis testing, each time adding a little more detail.

The general idea of hypothesis testing involves:

  • Making an initial assumption.
  • Collecting evidence (data).
  • Based on the available evidence (data), deciding whether to reject or not reject the initial assumption.

Every hypothesis test — regardless of the population parameter involved — requires the above three steps.

Example S.3.1

Is normal body temperature really 98.6 degrees f section  .

Consider the population of many, many adults. A researcher hypothesized that the average adult body temperature is lower than the often-advertised 98.6 degrees F. That is, the researcher wants an answer to the question: "Is the average adult body temperature 98.6 degrees? Or is it lower?" To answer his research question, the researcher starts by assuming that the average adult body temperature was 98.6 degrees F.

Then, the researcher went out and tried to find evidence that refutes his initial assumption. In doing so, he selects a random sample of 130 adults. The average body temperature of the 130 sampled adults is 98.25 degrees.

Then, the researcher uses the data he collected to make a decision about his initial assumption. It is either likely or unlikely that the researcher would collect the evidence he did given his initial assumption that the average adult body temperature is 98.6 degrees:

  • If it is likely , then the researcher does not reject his initial assumption that the average adult body temperature is 98.6 degrees. There is not enough evidence to do otherwise.
  • either the researcher's initial assumption is correct and he experienced a very unusual event;
  • or the researcher's initial assumption is incorrect.

In statistics, we generally don't make claims that require us to believe that a very unusual event happened. That is, in the practice of statistics, if the evidence (data) we collected is unlikely in light of the initial assumption, then we reject our initial assumption.

Example S.3.2

Criminal trial analogy section  .

One place where you can consistently see the general idea of hypothesis testing in action is in criminal trials held in the United States. Our criminal justice system assumes "the defendant is innocent until proven guilty." That is, our initial assumption is that the defendant is innocent.

In the practice of statistics, we make our initial assumption when we state our two competing hypotheses -- the null hypothesis ( H 0 ) and the alternative hypothesis ( H A ). Here, our hypotheses are:

  • H 0 : Defendant is not guilty (innocent)
  • H A : Defendant is guilty

In statistics, we always assume the null hypothesis is true . That is, the null hypothesis is always our initial assumption.

The prosecution team then collects evidence — such as finger prints, blood spots, hair samples, carpet fibers, shoe prints, ransom notes, and handwriting samples — with the hopes of finding "sufficient evidence" to make the assumption of innocence refutable.

In statistics, the data are the evidence.

The jury then makes a decision based on the available evidence:

  • If the jury finds sufficient evidence — beyond a reasonable doubt — to make the assumption of innocence refutable, the jury rejects the null hypothesis and deems the defendant guilty. We behave as if the defendant is guilty.
  • If there is insufficient evidence, then the jury does not reject the null hypothesis . We behave as if the defendant is innocent.

In statistics, we always make one of two decisions. We either "reject the null hypothesis" or we "fail to reject the null hypothesis."

Errors in Hypothesis Testing Section  

Did you notice the use of the phrase "behave as if" in the previous discussion? We "behave as if" the defendant is guilty; we do not "prove" that the defendant is guilty. And, we "behave as if" the defendant is innocent; we do not "prove" that the defendant is innocent.

This is a very important distinction! We make our decision based on evidence not on 100% guaranteed proof. Again:

  • If we reject the null hypothesis, we do not prove that the alternative hypothesis is true.
  • If we do not reject the null hypothesis, we do not prove that the null hypothesis is true.

We merely state that there is enough evidence to behave one way or the other. This is always true in statistics! Because of this, whatever the decision, there is always a chance that we made an error .

Let's review the two types of errors that can be made in criminal trials:

Table S.3.2 shows how this corresponds to the two types of errors in hypothesis testing.

Note that, in statistics, we call the two types of errors by two different  names -- one is called a "Type I error," and the other is called  a "Type II error." Here are the formal definitions of the two types of errors:

There is always a chance of making one of these errors. But, a good scientific study will minimize the chance of doing so!

Making the Decision Section  

Recall that it is either likely or unlikely that we would observe the evidence we did given our initial assumption. If it is likely , we do not reject the null hypothesis. If it is unlikely , then we reject the null hypothesis in favor of the alternative hypothesis. Effectively, then, making the decision reduces to determining "likely" or "unlikely."

In statistics, there are two ways to determine whether the evidence is likely or unlikely given the initial assumption:

  • We could take the " critical value approach " (favored in many of the older textbooks).
  • Or, we could take the " P -value approach " (what is used most often in research, journal articles, and statistical software).

In the next two sections, we review the procedures behind each of these two approaches. To make our review concrete, let's imagine that μ is the average grade point average of all American students who major in mathematics. We first review the critical value approach for conducting each of the following three hypothesis tests about the population mean $\mu$:

In Practice

  • We would want to conduct the first hypothesis test if we were interested in concluding that the average grade point average of the group is more than 3.
  • We would want to conduct the second hypothesis test if we were interested in concluding that the average grade point average of the group is less than 3.
  • And, we would want to conduct the third hypothesis test if we were only interested in concluding that the average grade point average of the group differs from 3 (without caring whether it is more or less than 3).

Upon completing the review of the critical value approach, we review the P -value approach for conducting each of the above three hypothesis tests about the population mean \(\mu\). The procedures that we review here for both approaches easily extend to hypothesis tests about any other population parameter.

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Hypothesis Testing

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A hypothesis test is a statistical inference method used to test the significance of a proposed (hypothesized) relation between population statistics (parameters) and their corresponding sample estimators . In other words, hypothesis tests are used to determine if there is enough evidence in a sample to prove a hypothesis true for the entire population.

The test considers two hypotheses: the null hypothesis , which is a statement meant to be tested, usually something like "there is no effect" with the intention of proving this false, and the alternate hypothesis , which is the statement meant to stand after the test is performed. The two hypotheses must be mutually exclusive ; moreover, in most applications, the two are complementary (one being the negation of the other). The test works by comparing the \(p\)-value to the level of significance (a chosen target). If the \(p\)-value is less than or equal to the level of significance, then the null hypothesis is rejected.

When analyzing data, only samples of a certain size might be manageable as efficient computations. In some situations the error terms follow a continuous or infinite distribution, hence the use of samples to suggest accuracy of the chosen test statistics. The method of hypothesis testing gives an advantage over guessing what distribution or which parameters the data follows.

Definitions and Methodology

Hypothesis test and confidence intervals.

In statistical inference, properties (parameters) of a population are analyzed by sampling data sets. Given assumptions on the distribution, i.e. a statistical model of the data, certain hypotheses can be deduced from the known behavior of the model. These hypotheses must be tested against sampled data from the population.

The null hypothesis \((\)denoted \(H_0)\) is a statement that is assumed to be true. If the null hypothesis is rejected, then there is enough evidence (statistical significance) to accept the alternate hypothesis \((\)denoted \(H_1).\) Before doing any test for significance, both hypotheses must be clearly stated and non-conflictive, i.e. mutually exclusive, statements. Rejecting the null hypothesis, given that it is true, is called a type I error and it is denoted \(\alpha\), which is also its probability of occurrence. Failing to reject the null hypothesis, given that it is false, is called a type II error and it is denoted \(\beta\), which is also its probability of occurrence. Also, \(\alpha\) is known as the significance level , and \(1-\beta\) is known as the power of the test. \(H_0\) \(\textbf{is true}\)\(\hspace{15mm}\) \(H_0\) \(\textbf{is false}\) \(\textbf{Reject}\) \(H_0\)\(\hspace{10mm}\) Type I error Correct Decision \(\textbf{Reject}\) \(H_1\) Correct Decision Type II error The test statistic is the standardized value following the sampled data under the assumption that the null hypothesis is true, and a chosen particular test. These tests depend on the statistic to be studied and the assumed distribution it follows, e.g. the population mean following a normal distribution. The \(p\)-value is the probability of observing an extreme test statistic in the direction of the alternate hypothesis, given that the null hypothesis is true. The critical value is the value of the assumed distribution of the test statistic such that the probability of making a type I error is small.
Methodologies: Given an estimator \(\hat \theta\) of a population statistic \(\theta\), following a probability distribution \(P(T)\), computed from a sample \(\mathcal{S},\) and given a significance level \(\alpha\) and test statistic \(t^*,\) define \(H_0\) and \(H_1;\) compute the test statistic \(t^*.\) \(p\)-value Approach (most prevalent): Find the \(p\)-value using \(t^*\) (right-tailed). If the \(p\)-value is at most \(\alpha,\) reject \(H_0\). Otherwise, reject \(H_1\). Critical Value Approach: Find the critical value solving the equation \(P(T\geq t_\alpha)=\alpha\) (right-tailed). If \(t^*>t_\alpha\), reject \(H_0\). Otherwise, reject \(H_1\). Note: Failing to reject \(H_0\) only means inability to accept \(H_1\), and it does not mean to accept \(H_0\).
Assume a normally distributed population has recorded cholesterol levels with various statistics computed. From a sample of 100 subjects in the population, the sample mean was 214.12 mg/dL (milligrams per deciliter), with a sample standard deviation of 45.71 mg/dL. Perform a hypothesis test, with significance level 0.05, to test if there is enough evidence to conclude that the population mean is larger than 200 mg/dL. Hypothesis Test We will perform a hypothesis test using the \(p\)-value approach with significance level \(\alpha=0.05:\) Define \(H_0\): \(\mu=200\). Define \(H_1\): \(\mu>200\). Since our values are normally distributed, the test statistic is \(z^*=\frac{\bar X - \mu_0}{\frac{s}{\sqrt{n}}}=\frac{214.12 - 200}{\frac{45.71}{\sqrt{100}}}\approx 3.09\). Using a standard normal distribution, we find that our \(p\)-value is approximately \(0.001\). Since the \(p\)-value is at most \(\alpha=0.05,\) we reject \(H_0\). Therefore, we can conclude that the test shows sufficient evidence to support the claim that \(\mu\) is larger than \(200\) mg/dL.

If the sample size was smaller, the normal and \(t\)-distributions behave differently. Also, the question itself must be managed by a double-tail test instead.

Assume a population's cholesterol levels are recorded and various statistics are computed. From a sample of 25 subjects, the sample mean was 214.12 mg/dL (milligrams per deciliter), with a sample standard deviation of 45.71 mg/dL. Perform a hypothesis test, with significance level 0.05, to test if there is enough evidence to conclude that the population mean is not equal to 200 mg/dL. Hypothesis Test We will perform a hypothesis test using the \(p\)-value approach with significance level \(\alpha=0.05\) and the \(t\)-distribution with 24 degrees of freedom: Define \(H_0\): \(\mu=200\). Define \(H_1\): \(\mu\neq 200\). Using the \(t\)-distribution, the test statistic is \(t^*=\frac{\bar X - \mu_0}{\frac{s}{\sqrt{n}}}=\frac{214.12 - 200}{\frac{45.71}{\sqrt{25}}}\approx 1.54\). Using a \(t\)-distribution with 24 degrees of freedom, we find that our \(p\)-value is approximately \(2(0.068)=0.136\). We have multiplied by two since this is a two-tailed argument, i.e. the mean can be smaller than or larger than. Since the \(p\)-value is larger than \(\alpha=0.05,\) we fail to reject \(H_0\). Therefore, the test does not show sufficient evidence to support the claim that \(\mu\) is not equal to \(200\) mg/dL.

The complement of the rejection on a two-tailed hypothesis test (with significance level \(\alpha\)) for a population parameter \(\theta\) is equivalent to finding a confidence interval \((\)with confidence level \(1-\alpha)\) for the population parameter \(\theta\). If the assumption on the parameter \(\theta\) falls inside the confidence interval, then the test has failed to reject the null hypothesis \((\)with \(p\)-value greater than \(\alpha).\) Otherwise, if \(\theta\) does not fall in the confidence interval, then the null hypothesis is rejected in favor of the alternate \((\)with \(p\)-value at most \(\alpha).\)

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Hypothesis Testing

Hypothesis testing is a tool for making statistical inferences about the population data. It is an analysis tool that tests assumptions and determines how likely something is within a given standard of accuracy. Hypothesis testing provides a way to verify whether the results of an experiment are valid.

A null hypothesis and an alternative hypothesis are set up before performing the hypothesis testing. This helps to arrive at a conclusion regarding the sample obtained from the population. In this article, we will learn more about hypothesis testing, its types, steps to perform the testing, and associated examples.

What is Hypothesis Testing in Statistics?

Hypothesis testing uses sample data from the population to draw useful conclusions regarding the population probability distribution . It tests an assumption made about the data using different types of hypothesis testing methodologies. The hypothesis testing results in either rejecting or not rejecting the null hypothesis.

Hypothesis Testing Definition

Hypothesis testing can be defined as a statistical tool that is used to identify if the results of an experiment are meaningful or not. It involves setting up a null hypothesis and an alternative hypothesis. These two hypotheses will always be mutually exclusive. This means that if the null hypothesis is true then the alternative hypothesis is false and vice versa. An example of hypothesis testing is setting up a test to check if a new medicine works on a disease in a more efficient manner.

Null Hypothesis

The null hypothesis is a concise mathematical statement that is used to indicate that there is no difference between two possibilities. In other words, there is no difference between certain characteristics of data. This hypothesis assumes that the outcomes of an experiment are based on chance alone. It is denoted as \(H_{0}\). Hypothesis testing is used to conclude if the null hypothesis can be rejected or not. Suppose an experiment is conducted to check if girls are shorter than boys at the age of 5. The null hypothesis will say that they are the same height.

Alternative Hypothesis

The alternative hypothesis is an alternative to the null hypothesis. It is used to show that the observations of an experiment are due to some real effect. It indicates that there is a statistical significance between two possible outcomes and can be denoted as \(H_{1}\) or \(H_{a}\). For the above-mentioned example, the alternative hypothesis would be that girls are shorter than boys at the age of 5.

Hypothesis Testing P Value

In hypothesis testing, the p value is used to indicate whether the results obtained after conducting a test are statistically significant or not. It also indicates the probability of making an error in rejecting or not rejecting the null hypothesis.This value is always a number between 0 and 1. The p value is compared to an alpha level, \(\alpha\) or significance level. The alpha level can be defined as the acceptable risk of incorrectly rejecting the null hypothesis. The alpha level is usually chosen between 1% to 5%.

Hypothesis Testing Critical region

All sets of values that lead to rejecting the null hypothesis lie in the critical region. Furthermore, the value that separates the critical region from the non-critical region is known as the critical value.

Hypothesis Testing Formula

Depending upon the type of data available and the size, different types of hypothesis testing are used to determine whether the null hypothesis can be rejected or not. The hypothesis testing formula for some important test statistics are given below:

  • z = \(\frac{\overline{x}-\mu}{\frac{\sigma}{\sqrt{n}}}\). \(\overline{x}\) is the sample mean, \(\mu\) is the population mean, \(\sigma\) is the population standard deviation and n is the size of the sample.
  • t = \(\frac{\overline{x}-\mu}{\frac{s}{\sqrt{n}}}\). s is the sample standard deviation.
  • \(\chi ^{2} = \sum \frac{(O_{i}-E_{i})^{2}}{E_{i}}\). \(O_{i}\) is the observed value and \(E_{i}\) is the expected value.

We will learn more about these test statistics in the upcoming section.

Types of Hypothesis Testing

Selecting the correct test for performing hypothesis testing can be confusing. These tests are used to determine a test statistic on the basis of which the null hypothesis can either be rejected or not rejected. Some of the important tests used for hypothesis testing are given below.

Hypothesis Testing Z Test

A z test is a way of hypothesis testing that is used for a large sample size (n ≥ 30). It is used to determine whether there is a difference between the population mean and the sample mean when the population standard deviation is known. It can also be used to compare the mean of two samples. It is used to compute the z test statistic. The formulas are given as follows:

  • One sample: z = \(\frac{\overline{x}-\mu}{\frac{\sigma}{\sqrt{n}}}\).
  • Two samples: z = \(\frac{(\overline{x_{1}}-\overline{x_{2}})-(\mu_{1}-\mu_{2})}{\sqrt{\frac{\sigma_{1}^{2}}{n_{1}}+\frac{\sigma_{2}^{2}}{n_{2}}}}\).

Hypothesis Testing t Test

The t test is another method of hypothesis testing that is used for a small sample size (n < 30). It is also used to compare the sample mean and population mean. However, the population standard deviation is not known. Instead, the sample standard deviation is known. The mean of two samples can also be compared using the t test.

  • One sample: t = \(\frac{\overline{x}-\mu}{\frac{s}{\sqrt{n}}}\).
  • Two samples: t = \(\frac{(\overline{x_{1}}-\overline{x_{2}})-(\mu_{1}-\mu_{2})}{\sqrt{\frac{s_{1}^{2}}{n_{1}}+\frac{s_{2}^{2}}{n_{2}}}}\).

Hypothesis Testing Chi Square

The Chi square test is a hypothesis testing method that is used to check whether the variables in a population are independent or not. It is used when the test statistic is chi-squared distributed.

One Tailed Hypothesis Testing

One tailed hypothesis testing is done when the rejection region is only in one direction. It can also be known as directional hypothesis testing because the effects can be tested in one direction only. This type of testing is further classified into the right tailed test and left tailed test.

Right Tailed Hypothesis Testing

The right tail test is also known as the upper tail test. This test is used to check whether the population parameter is greater than some value. The null and alternative hypotheses for this test are given as follows:

\(H_{0}\): The population parameter is ≤ some value

\(H_{1}\): The population parameter is > some value.

If the test statistic has a greater value than the critical value then the null hypothesis is rejected

Right Tail Hypothesis Testing

Left Tailed Hypothesis Testing

The left tail test is also known as the lower tail test. It is used to check whether the population parameter is less than some value. The hypotheses for this hypothesis testing can be written as follows:

\(H_{0}\): The population parameter is ≥ some value

\(H_{1}\): The population parameter is < some value.

The null hypothesis is rejected if the test statistic has a value lesser than the critical value.

Left Tail Hypothesis Testing

Two Tailed Hypothesis Testing

In this hypothesis testing method, the critical region lies on both sides of the sampling distribution. It is also known as a non - directional hypothesis testing method. The two-tailed test is used when it needs to be determined if the population parameter is assumed to be different than some value. The hypotheses can be set up as follows:

\(H_{0}\): the population parameter = some value

\(H_{1}\): the population parameter ≠ some value

The null hypothesis is rejected if the test statistic has a value that is not equal to the critical value.

Two Tail Hypothesis Testing

Hypothesis Testing Steps

Hypothesis testing can be easily performed in five simple steps. The most important step is to correctly set up the hypotheses and identify the right method for hypothesis testing. The basic steps to perform hypothesis testing are as follows:

  • Step 1: Set up the null hypothesis by correctly identifying whether it is the left-tailed, right-tailed, or two-tailed hypothesis testing.
  • Step 2: Set up the alternative hypothesis.
  • Step 3: Choose the correct significance level, \(\alpha\), and find the critical value.
  • Step 4: Calculate the correct test statistic (z, t or \(\chi\)) and p-value.
  • Step 5: Compare the test statistic with the critical value or compare the p-value with \(\alpha\) to arrive at a conclusion. In other words, decide if the null hypothesis is to be rejected or not.

Hypothesis Testing Example

The best way to solve a problem on hypothesis testing is by applying the 5 steps mentioned in the previous section. Suppose a researcher claims that the mean average weight of men is greater than 100kgs with a standard deviation of 15kgs. 30 men are chosen with an average weight of 112.5 Kgs. Using hypothesis testing, check if there is enough evidence to support the researcher's claim. The confidence interval is given as 95%.

Step 1: This is an example of a right-tailed test. Set up the null hypothesis as \(H_{0}\): \(\mu\) = 100.

Step 2: The alternative hypothesis is given by \(H_{1}\): \(\mu\) > 100.

Step 3: As this is a one-tailed test, \(\alpha\) = 100% - 95% = 5%. This can be used to determine the critical value.

1 - \(\alpha\) = 1 - 0.05 = 0.95

0.95 gives the required area under the curve. Now using a normal distribution table, the area 0.95 is at z = 1.645. A similar process can be followed for a t-test. The only additional requirement is to calculate the degrees of freedom given by n - 1.

Step 4: Calculate the z test statistic. This is because the sample size is 30. Furthermore, the sample and population means are known along with the standard deviation.

z = \(\frac{\overline{x}-\mu}{\frac{\sigma}{\sqrt{n}}}\).

\(\mu\) = 100, \(\overline{x}\) = 112.5, n = 30, \(\sigma\) = 15

z = \(\frac{112.5-100}{\frac{15}{\sqrt{30}}}\) = 4.56

Step 5: Conclusion. As 4.56 > 1.645 thus, the null hypothesis can be rejected.

Hypothesis Testing and Confidence Intervals

Confidence intervals form an important part of hypothesis testing. This is because the alpha level can be determined from a given confidence interval. Suppose a confidence interval is given as 95%. Subtract the confidence interval from 100%. This gives 100 - 95 = 5% or 0.05. This is the alpha value of a one-tailed hypothesis testing. To obtain the alpha value for a two-tailed hypothesis testing, divide this value by 2. This gives 0.05 / 2 = 0.025.

Related Articles:

  • Probability and Statistics
  • Data Handling

Important Notes on Hypothesis Testing

  • Hypothesis testing is a technique that is used to verify whether the results of an experiment are statistically significant.
  • It involves the setting up of a null hypothesis and an alternate hypothesis.
  • There are three types of tests that can be conducted under hypothesis testing - z test, t test, and chi square test.
  • Hypothesis testing can be classified as right tail, left tail, and two tail tests.

Examples on Hypothesis Testing

  • Example 1: The average weight of a dumbbell in a gym is 90lbs. However, a physical trainer believes that the average weight might be higher. A random sample of 5 dumbbells with an average weight of 110lbs and a standard deviation of 18lbs. Using hypothesis testing check if the physical trainer's claim can be supported for a 95% confidence level. Solution: As the sample size is lesser than 30, the t-test is used. \(H_{0}\): \(\mu\) = 90, \(H_{1}\): \(\mu\) > 90 \(\overline{x}\) = 110, \(\mu\) = 90, n = 5, s = 18. \(\alpha\) = 0.05 Using the t-distribution table, the critical value is 2.132 t = \(\frac{\overline{x}-\mu}{\frac{s}{\sqrt{n}}}\) t = 2.484 As 2.484 > 2.132, the null hypothesis is rejected. Answer: The average weight of the dumbbells may be greater than 90lbs
  • Example 2: The average score on a test is 80 with a standard deviation of 10. With a new teaching curriculum introduced it is believed that this score will change. On random testing, the score of 38 students, the mean was found to be 88. With a 0.05 significance level, is there any evidence to support this claim? Solution: This is an example of two-tail hypothesis testing. The z test will be used. \(H_{0}\): \(\mu\) = 80, \(H_{1}\): \(\mu\) ≠ 80 \(\overline{x}\) = 88, \(\mu\) = 80, n = 36, \(\sigma\) = 10. \(\alpha\) = 0.05 / 2 = 0.025 The critical value using the normal distribution table is 1.96 z = \(\frac{\overline{x}-\mu}{\frac{\sigma}{\sqrt{n}}}\) z = \(\frac{88-80}{\frac{10}{\sqrt{36}}}\) = 4.8 As 4.8 > 1.96, the null hypothesis is rejected. Answer: There is a difference in the scores after the new curriculum was introduced.
  • Example 3: The average score of a class is 90. However, a teacher believes that the average score might be lower. The scores of 6 students were randomly measured. The mean was 82 with a standard deviation of 18. With a 0.05 significance level use hypothesis testing to check if this claim is true. Solution: The t test will be used. \(H_{0}\): \(\mu\) = 90, \(H_{1}\): \(\mu\) < 90 \(\overline{x}\) = 110, \(\mu\) = 90, n = 6, s = 18 The critical value from the t table is -2.015 t = \(\frac{\overline{x}-\mu}{\frac{s}{\sqrt{n}}}\) t = \(\frac{82-90}{\frac{18}{\sqrt{6}}}\) t = -1.088 As -1.088 > -2.015, we fail to reject the null hypothesis. Answer: There is not enough evidence to support the claim.

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FAQs on Hypothesis Testing

What is hypothesis testing.

Hypothesis testing in statistics is a tool that is used to make inferences about the population data. It is also used to check if the results of an experiment are valid.

What is the z Test in Hypothesis Testing?

The z test in hypothesis testing is used to find the z test statistic for normally distributed data . The z test is used when the standard deviation of the population is known and the sample size is greater than or equal to 30.

What is the t Test in Hypothesis Testing?

The t test in hypothesis testing is used when the data follows a student t distribution . It is used when the sample size is less than 30 and standard deviation of the population is not known.

What is the formula for z test in Hypothesis Testing?

The formula for a one sample z test in hypothesis testing is z = \(\frac{\overline{x}-\mu}{\frac{\sigma}{\sqrt{n}}}\) and for two samples is z = \(\frac{(\overline{x_{1}}-\overline{x_{2}})-(\mu_{1}-\mu_{2})}{\sqrt{\frac{\sigma_{1}^{2}}{n_{1}}+\frac{\sigma_{2}^{2}}{n_{2}}}}\).

What is the p Value in Hypothesis Testing?

The p value helps to determine if the test results are statistically significant or not. In hypothesis testing, the null hypothesis can either be rejected or not rejected based on the comparison between the p value and the alpha level.

What is One Tail Hypothesis Testing?

When the rejection region is only on one side of the distribution curve then it is known as one tail hypothesis testing. The right tail test and the left tail test are two types of directional hypothesis testing.

What is the Alpha Level in Two Tail Hypothesis Testing?

To get the alpha level in a two tail hypothesis testing divide \(\alpha\) by 2. This is done as there are two rejection regions in the curve.

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StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2024 Jan-.

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Hypothesis testing, p values, confidence intervals, and significance.

Jacob Shreffler ; Martin R. Huecker .

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Last Update: March 13, 2023 .

  • Definition/Introduction

Medical providers often rely on evidence-based medicine to guide decision-making in practice. Often a research hypothesis is tested with results provided, typically with p values, confidence intervals, or both. Additionally, statistical or research significance is estimated or determined by the investigators. Unfortunately, healthcare providers may have different comfort levels in interpreting these findings, which may affect the adequate application of the data.

  • Issues of Concern

Without a foundational understanding of hypothesis testing, p values, confidence intervals, and the difference between statistical and clinical significance, it may affect healthcare providers' ability to make clinical decisions without relying purely on the research investigators deemed level of significance. Therefore, an overview of these concepts is provided to allow medical professionals to use their expertise to determine if results are reported sufficiently and if the study outcomes are clinically appropriate to be applied in healthcare practice.

Hypothesis Testing

Investigators conducting studies need research questions and hypotheses to guide analyses. Starting with broad research questions (RQs), investigators then identify a gap in current clinical practice or research. Any research problem or statement is grounded in a better understanding of relationships between two or more variables. For this article, we will use the following research question example:

Research Question: Is Drug 23 an effective treatment for Disease A?

Research questions do not directly imply specific guesses or predictions; we must formulate research hypotheses. A hypothesis is a predetermined declaration regarding the research question in which the investigator(s) makes a precise, educated guess about a study outcome. This is sometimes called the alternative hypothesis and ultimately allows the researcher to take a stance based on experience or insight from medical literature. An example of a hypothesis is below.

Research Hypothesis: Drug 23 will significantly reduce symptoms associated with Disease A compared to Drug 22.

The null hypothesis states that there is no statistical difference between groups based on the stated research hypothesis.

Researchers should be aware of journal recommendations when considering how to report p values, and manuscripts should remain internally consistent.

Regarding p values, as the number of individuals enrolled in a study (the sample size) increases, the likelihood of finding a statistically significant effect increases. With very large sample sizes, the p-value can be very low significant differences in the reduction of symptoms for Disease A between Drug 23 and Drug 22. The null hypothesis is deemed true until a study presents significant data to support rejecting the null hypothesis. Based on the results, the investigators will either reject the null hypothesis (if they found significant differences or associations) or fail to reject the null hypothesis (they could not provide proof that there were significant differences or associations).

To test a hypothesis, researchers obtain data on a representative sample to determine whether to reject or fail to reject a null hypothesis. In most research studies, it is not feasible to obtain data for an entire population. Using a sampling procedure allows for statistical inference, though this involves a certain possibility of error. [1]  When determining whether to reject or fail to reject the null hypothesis, mistakes can be made: Type I and Type II errors. Though it is impossible to ensure that these errors have not occurred, researchers should limit the possibilities of these faults. [2]

Significance

Significance is a term to describe the substantive importance of medical research. Statistical significance is the likelihood of results due to chance. [3]  Healthcare providers should always delineate statistical significance from clinical significance, a common error when reviewing biomedical research. [4]  When conceptualizing findings reported as either significant or not significant, healthcare providers should not simply accept researchers' results or conclusions without considering the clinical significance. Healthcare professionals should consider the clinical importance of findings and understand both p values and confidence intervals so they do not have to rely on the researchers to determine the level of significance. [5]  One criterion often used to determine statistical significance is the utilization of p values.

P values are used in research to determine whether the sample estimate is significantly different from a hypothesized value. The p-value is the probability that the observed effect within the study would have occurred by chance if, in reality, there was no true effect. Conventionally, data yielding a p<0.05 or p<0.01 is considered statistically significant. While some have debated that the 0.05 level should be lowered, it is still universally practiced. [6]  Hypothesis testing allows us to determine the size of the effect.

An example of findings reported with p values are below:

Statement: Drug 23 reduced patients' symptoms compared to Drug 22. Patients who received Drug 23 (n=100) were 2.1 times less likely than patients who received Drug 22 (n = 100) to experience symptoms of Disease A, p<0.05.

Statement:Individuals who were prescribed Drug 23 experienced fewer symptoms (M = 1.3, SD = 0.7) compared to individuals who were prescribed Drug 22 (M = 5.3, SD = 1.9). This finding was statistically significant, p= 0.02.

For either statement, if the threshold had been set at 0.05, the null hypothesis (that there was no relationship) should be rejected, and we should conclude significant differences. Noticeably, as can be seen in the two statements above, some researchers will report findings with < or > and others will provide an exact p-value (0.000001) but never zero [6] . When examining research, readers should understand how p values are reported. The best practice is to report all p values for all variables within a study design, rather than only providing p values for variables with significant findings. [7]  The inclusion of all p values provides evidence for study validity and limits suspicion for selective reporting/data mining.  

While researchers have historically used p values, experts who find p values problematic encourage the use of confidence intervals. [8] . P-values alone do not allow us to understand the size or the extent of the differences or associations. [3]  In March 2016, the American Statistical Association (ASA) released a statement on p values, noting that scientific decision-making and conclusions should not be based on a fixed p-value threshold (e.g., 0.05). They recommend focusing on the significance of results in the context of study design, quality of measurements, and validity of data. Ultimately, the ASA statement noted that in isolation, a p-value does not provide strong evidence. [9]

When conceptualizing clinical work, healthcare professionals should consider p values with a concurrent appraisal study design validity. For example, a p-value from a double-blinded randomized clinical trial (designed to minimize bias) should be weighted higher than one from a retrospective observational study [7] . The p-value debate has smoldered since the 1950s [10] , and replacement with confidence intervals has been suggested since the 1980s. [11]

Confidence Intervals

A confidence interval provides a range of values within given confidence (e.g., 95%), including the accurate value of the statistical constraint within a targeted population. [12]  Most research uses a 95% CI, but investigators can set any level (e.g., 90% CI, 99% CI). [13]  A CI provides a range with the lower bound and upper bound limits of a difference or association that would be plausible for a population. [14]  Therefore, a CI of 95% indicates that if a study were to be carried out 100 times, the range would contain the true value in 95, [15]  confidence intervals provide more evidence regarding the precision of an estimate compared to p-values. [6]

In consideration of the similar research example provided above, one could make the following statement with 95% CI:

Statement: Individuals who were prescribed Drug 23 had no symptoms after three days, which was significantly faster than those prescribed Drug 22; there was a mean difference between the two groups of days to the recovery of 4.2 days (95% CI: 1.9 – 7.8).

It is important to note that the width of the CI is affected by the standard error and the sample size; reducing a study sample number will result in less precision of the CI (increase the width). [14]  A larger width indicates a smaller sample size or a larger variability. [16]  A researcher would want to increase the precision of the CI. For example, a 95% CI of 1.43 – 1.47 is much more precise than the one provided in the example above. In research and clinical practice, CIs provide valuable information on whether the interval includes or excludes any clinically significant values. [14]

Null values are sometimes used for differences with CI (zero for differential comparisons and 1 for ratios). However, CIs provide more information than that. [15]  Consider this example: A hospital implements a new protocol that reduced wait time for patients in the emergency department by an average of 25 minutes (95% CI: -2.5 – 41 minutes). Because the range crosses zero, implementing this protocol in different populations could result in longer wait times; however, the range is much higher on the positive side. Thus, while the p-value used to detect statistical significance for this may result in "not significant" findings, individuals should examine this range, consider the study design, and weigh whether or not it is still worth piloting in their workplace.

Similarly to p-values, 95% CIs cannot control for researchers' errors (e.g., study bias or improper data analysis). [14]  In consideration of whether to report p-values or CIs, researchers should examine journal preferences. When in doubt, reporting both may be beneficial. [13]  An example is below:

Reporting both: Individuals who were prescribed Drug 23 had no symptoms after three days, which was significantly faster than those prescribed Drug 22, p = 0.009. There was a mean difference between the two groups of days to the recovery of 4.2 days (95% CI: 1.9 – 7.8).

  • Clinical Significance

Recall that clinical significance and statistical significance are two different concepts. Healthcare providers should remember that a study with statistically significant differences and large sample size may be of no interest to clinicians, whereas a study with smaller sample size and statistically non-significant results could impact clinical practice. [14]  Additionally, as previously mentioned, a non-significant finding may reflect the study design itself rather than relationships between variables.

Healthcare providers using evidence-based medicine to inform practice should use clinical judgment to determine the practical importance of studies through careful evaluation of the design, sample size, power, likelihood of type I and type II errors, data analysis, and reporting of statistical findings (p values, 95% CI or both). [4]  Interestingly, some experts have called for "statistically significant" or "not significant" to be excluded from work as statistical significance never has and will never be equivalent to clinical significance. [17]

The decision on what is clinically significant can be challenging, depending on the providers' experience and especially the severity of the disease. Providers should use their knowledge and experiences to determine the meaningfulness of study results and make inferences based not only on significant or insignificant results by researchers but through their understanding of study limitations and practical implications.

  • Nursing, Allied Health, and Interprofessional Team Interventions

All physicians, nurses, pharmacists, and other healthcare professionals should strive to understand the concepts in this chapter. These individuals should maintain the ability to review and incorporate new literature for evidence-based and safe care. 

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Disclosure: Jacob Shreffler declares no relevant financial relationships with ineligible companies.

Disclosure: Martin Huecker declares no relevant financial relationships with ineligible companies.

This book is distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) ( http://creativecommons.org/licenses/by-nc-nd/4.0/ ), which permits others to distribute the work, provided that the article is not altered or used commercially. You are not required to obtain permission to distribute this article, provided that you credit the author and journal.

  • Cite this Page Shreffler J, Huecker MR. Hypothesis Testing, P Values, Confidence Intervals, and Significance. [Updated 2023 Mar 13]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2024 Jan-.

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Unit 12: Significance tests (hypothesis testing)

About this unit, the idea of significance tests.

  • Simple hypothesis testing (Opens a modal)
  • Idea behind hypothesis testing (Opens a modal)
  • Examples of null and alternative hypotheses (Opens a modal)
  • P-values and significance tests (Opens a modal)
  • Comparing P-values to different significance levels (Opens a modal)
  • Estimating a P-value from a simulation (Opens a modal)
  • Using P-values to make conclusions (Opens a modal)
  • Simple hypothesis testing Get 3 of 4 questions to level up!
  • Writing null and alternative hypotheses Get 3 of 4 questions to level up!
  • Estimating P-values from simulations Get 3 of 4 questions to level up!

Error probabilities and power

  • Introduction to Type I and Type II errors (Opens a modal)
  • Type 1 errors (Opens a modal)
  • Examples identifying Type I and Type II errors (Opens a modal)
  • Introduction to power in significance tests (Opens a modal)
  • Examples thinking about power in significance tests (Opens a modal)
  • Consequences of errors and significance (Opens a modal)
  • Type I vs Type II error Get 3 of 4 questions to level up!
  • Error probabilities and power Get 3 of 4 questions to level up!

Tests about a population proportion

  • Constructing hypotheses for a significance test about a proportion (Opens a modal)
  • Conditions for a z test about a proportion (Opens a modal)
  • Reference: Conditions for inference on a proportion (Opens a modal)
  • Calculating a z statistic in a test about a proportion (Opens a modal)
  • Calculating a P-value given a z statistic (Opens a modal)
  • Making conclusions in a test about a proportion (Opens a modal)
  • Writing hypotheses for a test about a proportion Get 3 of 4 questions to level up!
  • Conditions for a z test about a proportion Get 3 of 4 questions to level up!
  • Calculating the test statistic in a z test for a proportion Get 3 of 4 questions to level up!
  • Calculating the P-value in a z test for a proportion Get 3 of 4 questions to level up!
  • Making conclusions in a z test for a proportion Get 3 of 4 questions to level up!

Tests about a population mean

  • Writing hypotheses for a significance test about a mean (Opens a modal)
  • Conditions for a t test about a mean (Opens a modal)
  • Reference: Conditions for inference on a mean (Opens a modal)
  • When to use z or t statistics in significance tests (Opens a modal)
  • Example calculating t statistic for a test about a mean (Opens a modal)
  • Using TI calculator for P-value from t statistic (Opens a modal)
  • Using a table to estimate P-value from t statistic (Opens a modal)
  • Comparing P-value from t statistic to significance level (Opens a modal)
  • Free response example: Significance test for a mean (Opens a modal)
  • Writing hypotheses for a test about a mean Get 3 of 4 questions to level up!
  • Conditions for a t test about a mean Get 3 of 4 questions to level up!
  • Calculating the test statistic in a t test for a mean Get 3 of 4 questions to level up!
  • Calculating the P-value in a t test for a mean Get 3 of 4 questions to level up!
  • Making conclusions in a t test for a mean Get 3 of 4 questions to level up!

More significance testing videos

  • Hypothesis testing and p-values (Opens a modal)
  • One-tailed and two-tailed tests (Opens a modal)
  • Z-statistics vs. T-statistics (Opens a modal)
  • Small sample hypothesis test (Opens a modal)
  • Large sample proportion hypothesis testing (Opens a modal)
  • Hypothesis Testing: Definition, Uses, Limitations + Examples

busayo.longe

Hypothesis testing is as old as the scientific method and is at the heart of the research process. 

Research exists to validate or disprove assumptions about various phenomena. The process of validation involves testing and it is in this context that we will explore hypothesis testing. 

What is a Hypothesis? 

A hypothesis is a calculated prediction or assumption about a population parameter based on limited evidence. The whole idea behind hypothesis formulation is testing—this means the researcher subjects his or her calculated assumption to a series of evaluations to know whether they are true or false. 

Typically, every research starts with a hypothesis—the investigator makes a claim and experiments to prove that this claim is true or false . For instance, if you predict that students who drink milk before class perform better than those who don’t, then this becomes a hypothesis that can be confirmed or refuted using an experiment.  

Read: What is Empirical Research Study? [Examples & Method]

What are the Types of Hypotheses? 

1. simple hypothesis.

Also known as a basic hypothesis, a simple hypothesis suggests that an independent variable is responsible for a corresponding dependent variable. In other words, an occurrence of the independent variable inevitably leads to an occurrence of the dependent variable. 

Typically, simple hypotheses are considered as generally true, and they establish a causal relationship between two variables. 

Examples of Simple Hypothesis  

  • Drinking soda and other sugary drinks can cause obesity. 
  • Smoking cigarettes daily leads to lung cancer.

2. Complex Hypothesis

A complex hypothesis is also known as a modal. It accounts for the causal relationship between two independent variables and the resulting dependent variables. This means that the combination of the independent variables leads to the occurrence of the dependent variables . 

Examples of Complex Hypotheses  

  • Adults who do not smoke and drink are less likely to develop liver-related conditions.
  • Global warming causes icebergs to melt which in turn causes major changes in weather patterns.

3. Null Hypothesis

As the name suggests, a null hypothesis is formed when a researcher suspects that there’s no relationship between the variables in an observation. In this case, the purpose of the research is to approve or disapprove this assumption. 

Examples of Null Hypothesis

  • This is no significant change in a student’s performance if they drink coffee or tea before classes. 
  • There’s no significant change in the growth of a plant if one uses distilled water only or vitamin-rich water. 
Read: Research Report: Definition, Types + [Writing Guide]

4. Alternative Hypothesis 

To disapprove a null hypothesis, the researcher has to come up with an opposite assumption—this assumption is known as the alternative hypothesis. This means if the null hypothesis says that A is false, the alternative hypothesis assumes that A is true. 

An alternative hypothesis can be directional or non-directional depending on the direction of the difference. A directional alternative hypothesis specifies the direction of the tested relationship, stating that one variable is predicted to be larger or smaller than the null value while a non-directional hypothesis only validates the existence of a difference without stating its direction. 

Examples of Alternative Hypotheses  

  • Starting your day with a cup of tea instead of a cup of coffee can make you more alert in the morning. 
  • The growth of a plant improves significantly when it receives distilled water instead of vitamin-rich water. 

5. Logical Hypothesis

Logical hypotheses are some of the most common types of calculated assumptions in systematic investigations. It is an attempt to use your reasoning to connect different pieces in research and build a theory using little evidence. In this case, the researcher uses any data available to him, to form a plausible assumption that can be tested. 

Examples of Logical Hypothesis

  • Waking up early helps you to have a more productive day. 
  • Beings from Mars would not be able to breathe the air in the atmosphere of the Earth. 

6. Empirical Hypothesis  

After forming a logical hypothesis, the next step is to create an empirical or working hypothesis. At this stage, your logical hypothesis undergoes systematic testing to prove or disprove the assumption. An empirical hypothesis is subject to several variables that can trigger changes and lead to specific outcomes. 

Examples of Empirical Testing 

  • People who eat more fish run faster than people who eat meat.
  • Women taking vitamin E grow hair faster than those taking vitamin K.

7. Statistical Hypothesis

When forming a statistical hypothesis, the researcher examines the portion of a population of interest and makes a calculated assumption based on the data from this sample. A statistical hypothesis is most common with systematic investigations involving a large target audience. Here, it’s impossible to collect responses from every member of the population so you have to depend on data from your sample and extrapolate the results to the wider population. 

Examples of Statistical Hypothesis  

  • 45% of students in Louisiana have middle-income parents. 
  • 80% of the UK’s population gets a divorce because of irreconcilable differences.

What is Hypothesis Testing? 

Hypothesis testing is an assessment method that allows researchers to determine the plausibility of a hypothesis. It involves testing an assumption about a specific population parameter to know whether it’s true or false. These population parameters include variance, standard deviation, and median. 

Typically, hypothesis testing starts with developing a null hypothesis and then performing several tests that support or reject the null hypothesis. The researcher uses test statistics to compare the association or relationship between two or more variables. 

Explore: Research Bias: Definition, Types + Examples

Researchers also use hypothesis testing to calculate the coefficient of variation and determine if the regression relationship and the correlation coefficient are statistically significant.

How Hypothesis Testing Works

The basis of hypothesis testing is to examine and analyze the null hypothesis and alternative hypothesis to know which one is the most plausible assumption. Since both assumptions are mutually exclusive, only one can be true. In other words, the occurrence of a null hypothesis destroys the chances of the alternative coming to life, and vice-versa. 

Interesting: 21 Chrome Extensions for Academic Researchers in 2021

What Are The Stages of Hypothesis Testing?  

To successfully confirm or refute an assumption, the researcher goes through five (5) stages of hypothesis testing; 

  • Determine the null hypothesis
  • Specify the alternative hypothesis
  • Set the significance level
  • Calculate the test statistics and corresponding P-value
  • Draw your conclusion
  • Determine the Null Hypothesis

Like we mentioned earlier, hypothesis testing starts with creating a null hypothesis which stands as an assumption that a certain statement is false or implausible. For example, the null hypothesis (H0) could suggest that different subgroups in the research population react to a variable in the same way. 

  • Specify the Alternative Hypothesis

Once you know the variables for the null hypothesis, the next step is to determine the alternative hypothesis. The alternative hypothesis counters the null assumption by suggesting the statement or assertion is true. Depending on the purpose of your research, the alternative hypothesis can be one-sided or two-sided. 

Using the example we established earlier, the alternative hypothesis may argue that the different sub-groups react differently to the same variable based on several internal and external factors. 

  • Set the Significance Level

Many researchers create a 5% allowance for accepting the value of an alternative hypothesis, even if the value is untrue. This means that there is a 0.05 chance that one would go with the value of the alternative hypothesis, despite the truth of the null hypothesis. 

Something to note here is that the smaller the significance level, the greater the burden of proof needed to reject the null hypothesis and support the alternative hypothesis.

Explore: What is Data Interpretation? + [Types, Method & Tools]
  • Calculate the Test Statistics and Corresponding P-Value 

Test statistics in hypothesis testing allow you to compare different groups between variables while the p-value accounts for the probability of obtaining sample statistics if your null hypothesis is true. In this case, your test statistics can be the mean, median and similar parameters. 

If your p-value is 0.65, for example, then it means that the variable in your hypothesis will happen 65 in100 times by pure chance. Use this formula to determine the p-value for your data: 

hypothesis testing definition

  • Draw Your Conclusions

After conducting a series of tests, you should be able to agree or refute the hypothesis based on feedback and insights from your sample data.  

Applications of Hypothesis Testing in Research

Hypothesis testing isn’t only confined to numbers and calculations; it also has several real-life applications in business, manufacturing, advertising, and medicine. 

In a factory or other manufacturing plants, hypothesis testing is an important part of quality and production control before the final products are approved and sent out to the consumer. 

During ideation and strategy development, C-level executives use hypothesis testing to evaluate their theories and assumptions before any form of implementation. For example, they could leverage hypothesis testing to determine whether or not some new advertising campaign, marketing technique, etc. causes increased sales. 

In addition, hypothesis testing is used during clinical trials to prove the efficacy of a drug or new medical method before its approval for widespread human usage. 

What is an Example of Hypothesis Testing?

An employer claims that her workers are of above-average intelligence. She takes a random sample of 20 of them and gets the following results: 

Mean IQ Scores: 110

Standard Deviation: 15 

Mean Population IQ: 100

Step 1: Using the value of the mean population IQ, we establish the null hypothesis as 100.

Step 2: State that the alternative hypothesis is greater than 100.

Step 3: State the alpha level as 0.05 or 5% 

Step 4: Find the rejection region area (given by your alpha level above) from the z-table. An area of .05 is equal to a z-score of 1.645.

Step 5: Calculate the test statistics using this formula

hypothesis testing definition

Z = (110–100) ÷ (15÷√20) 

10 ÷ 3.35 = 2.99 

If the value of the test statistics is higher than the value of the rejection region, then you should reject the null hypothesis. If it is less, then you cannot reject the null. 

In this case, 2.99 > 1.645 so we reject the null. 

Importance/Benefits of Hypothesis Testing 

The most significant benefit of hypothesis testing is it allows you to evaluate the strength of your claim or assumption before implementing it in your data set. Also, hypothesis testing is the only valid method to prove that something “is or is not”. Other benefits include: 

  • Hypothesis testing provides a reliable framework for making any data decisions for your population of interest. 
  • It helps the researcher to successfully extrapolate data from the sample to the larger population. 
  • Hypothesis testing allows the researcher to determine whether the data from the sample is statistically significant. 
  • Hypothesis testing is one of the most important processes for measuring the validity and reliability of outcomes in any systematic investigation. 
  • It helps to provide links to the underlying theory and specific research questions.

Criticism and Limitations of Hypothesis Testing

Several limitations of hypothesis testing can affect the quality of data you get from this process. Some of these limitations include: 

  • The interpretation of a p-value for observation depends on the stopping rule and definition of multiple comparisons. This makes it difficult to calculate since the stopping rule is subject to numerous interpretations, plus “multiple comparisons” are unavoidably ambiguous. 
  • Conceptual issues often arise in hypothesis testing, especially if the researcher merges Fisher and Neyman-Pearson’s methods which are conceptually distinct. 
  • In an attempt to focus on the statistical significance of the data, the researcher might ignore the estimation and confirmation by repeated experiments.
  • Hypothesis testing can trigger publication bias, especially when it requires statistical significance as a criterion for publication.
  • When used to detect whether a difference exists between groups, hypothesis testing can trigger absurd assumptions that affect the reliability of your observation.

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Lesson 10 of 24 By Avijeet Biswal

A Complete Guide on Hypothesis Testing in Statistics

Table of Contents

In today’s data-driven world , decisions are based on data all the time. Hypothesis plays a crucial role in that process, whether it may be making business decisions, in the health sector, academia, or in quality improvement. Without hypothesis & hypothesis tests, you risk drawing the wrong conclusions and making bad decisions. In this tutorial, you will look at Hypothesis Testing in Statistics.

What Is Hypothesis Testing in Statistics?

Hypothesis Testing is a type of statistical analysis in which you put your assumptions about a population parameter to the test. It is used to estimate the relationship between 2 statistical variables.

Let's discuss few examples of statistical hypothesis from real-life - 

  • A teacher assumes that 60% of his college's students come from lower-middle-class families.
  • A doctor believes that 3D (Diet, Dose, and Discipline) is 90% effective for diabetic patients.

Now that you know about hypothesis testing, look at the two types of hypothesis testing in statistics.

Hypothesis Testing Formula

Z = ( x̅ – μ0 ) / (σ /√n)

  • Here, x̅ is the sample mean,
  • μ0 is the population mean,
  • σ is the standard deviation,
  • n is the sample size.

How Hypothesis Testing Works?

An analyst performs hypothesis testing on a statistical sample to present evidence of the plausibility of the null hypothesis. Measurements and analyses are conducted on a random sample of the population to test a theory. Analysts use a random population sample to test two hypotheses: the null and alternative hypotheses.

The null hypothesis is typically an equality hypothesis between population parameters; for example, a null hypothesis may claim that the population means return equals zero. The alternate hypothesis is essentially the inverse of the null hypothesis (e.g., the population means the return is not equal to zero). As a result, they are mutually exclusive, and only one can be correct. One of the two possibilities, however, will always be correct.

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Null Hypothesis and Alternate Hypothesis

The Null Hypothesis is the assumption that the event will not occur. A null hypothesis has no bearing on the study's outcome unless it is rejected.

H0 is the symbol for it, and it is pronounced H-naught.

The Alternate Hypothesis is the logical opposite of the null hypothesis. The acceptance of the alternative hypothesis follows the rejection of the null hypothesis. H1 is the symbol for it.

Let's understand this with an example.

A sanitizer manufacturer claims that its product kills 95 percent of germs on average. 

To put this company's claim to the test, create a null and alternate hypothesis.

H0 (Null Hypothesis): Average = 95%.

Alternative Hypothesis (H1): The average is less than 95%.

Another straightforward example to understand this concept is determining whether or not a coin is fair and balanced. The null hypothesis states that the probability of a show of heads is equal to the likelihood of a show of tails. In contrast, the alternate theory states that the probability of a show of heads and tails would be very different.

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Hypothesis Testing Calculation With Examples

Let's consider a hypothesis test for the average height of women in the United States. Suppose our null hypothesis is that the average height is 5'4". We gather a sample of 100 women and determine that their average height is 5'5". The standard deviation of population is 2.

To calculate the z-score, we would use the following formula:

z = ( x̅ – μ0 ) / (σ /√n)

z = (5'5" - 5'4") / (2" / √100)

z = 0.5 / (0.045)

 We will reject the null hypothesis as the z-score of 11.11 is very large and conclude that there is evidence to suggest that the average height of women in the US is greater than 5'4".

Steps of Hypothesis Testing

Step 1: specify your null and alternate hypotheses.

It is critical to rephrase your original research hypothesis (the prediction that you wish to study) as a null (Ho) and alternative (Ha) hypothesis so that you can test it quantitatively. Your first hypothesis, which predicts a link between variables, is generally your alternate hypothesis. The null hypothesis predicts no link between the variables of interest.

Step 2: Gather Data

For a statistical test to be legitimate, sampling and data collection must be done in a way that is meant to test your hypothesis. You cannot draw statistical conclusions about the population you are interested in if your data is not representative.

Step 3: Conduct a Statistical Test

Other statistical tests are available, but they all compare within-group variance (how to spread out the data inside a category) against between-group variance (how different the categories are from one another). If the between-group variation is big enough that there is little or no overlap between groups, your statistical test will display a low p-value to represent this. This suggests that the disparities between these groups are unlikely to have occurred by accident. Alternatively, if there is a large within-group variance and a low between-group variance, your statistical test will show a high p-value. Any difference you find across groups is most likely attributable to chance. The variety of variables and the level of measurement of your obtained data will influence your statistical test selection.

Step 4: Determine Rejection Of Your Null Hypothesis

Your statistical test results must determine whether your null hypothesis should be rejected or not. In most circumstances, you will base your judgment on the p-value provided by the statistical test. In most circumstances, your preset level of significance for rejecting the null hypothesis will be 0.05 - that is, when there is less than a 5% likelihood that these data would be seen if the null hypothesis were true. In other circumstances, researchers use a lower level of significance, such as 0.01 (1%). This reduces the possibility of wrongly rejecting the null hypothesis.

Step 5: Present Your Results 

The findings of hypothesis testing will be discussed in the results and discussion portions of your research paper, dissertation, or thesis. You should include a concise overview of the data and a summary of the findings of your statistical test in the results section. You can talk about whether your results confirmed your initial hypothesis or not in the conversation. Rejecting or failing to reject the null hypothesis is a formal term used in hypothesis testing. This is likely a must for your statistics assignments.

Types of Hypothesis Testing

To determine whether a discovery or relationship is statistically significant, hypothesis testing uses a z-test. It usually checks to see if two means are the same (the null hypothesis). Only when the population standard deviation is known and the sample size is 30 data points or more, can a z-test be applied.

A statistical test called a t-test is employed to compare the means of two groups. To determine whether two groups differ or if a procedure or treatment affects the population of interest, it is frequently used in hypothesis testing.

Chi-Square 

You utilize a Chi-square test for hypothesis testing concerning whether your data is as predicted. To determine if the expected and observed results are well-fitted, the Chi-square test analyzes the differences between categorical variables from a random sample. The test's fundamental premise is that the observed values in your data should be compared to the predicted values that would be present if the null hypothesis were true.

Hypothesis Testing and Confidence Intervals

Both confidence intervals and hypothesis tests are inferential techniques that depend on approximating the sample distribution. Data from a sample is used to estimate a population parameter using confidence intervals. Data from a sample is used in hypothesis testing to examine a given hypothesis. We must have a postulated parameter to conduct hypothesis testing.

Bootstrap distributions and randomization distributions are created using comparable simulation techniques. The observed sample statistic is the focal point of a bootstrap distribution, whereas the null hypothesis value is the focal point of a randomization distribution.

A variety of feasible population parameter estimates are included in confidence ranges. In this lesson, we created just two-tailed confidence intervals. There is a direct connection between these two-tail confidence intervals and these two-tail hypothesis tests. The results of a two-tailed hypothesis test and two-tailed confidence intervals typically provide the same results. In other words, a hypothesis test at the 0.05 level will virtually always fail to reject the null hypothesis if the 95% confidence interval contains the predicted value. A hypothesis test at the 0.05 level will nearly certainly reject the null hypothesis if the 95% confidence interval does not include the hypothesized parameter.

Simple and Composite Hypothesis Testing

Depending on the population distribution, you can classify the statistical hypothesis into two types.

Simple Hypothesis: A simple hypothesis specifies an exact value for the parameter.

Composite Hypothesis: A composite hypothesis specifies a range of values.

A company is claiming that their average sales for this quarter are 1000 units. This is an example of a simple hypothesis.

Suppose the company claims that the sales are in the range of 900 to 1000 units. Then this is a case of a composite hypothesis.

One-Tailed and Two-Tailed Hypothesis Testing

The One-Tailed test, also called a directional test, considers a critical region of data that would result in the null hypothesis being rejected if the test sample falls into it, inevitably meaning the acceptance of the alternate hypothesis.

In a one-tailed test, the critical distribution area is one-sided, meaning the test sample is either greater or lesser than a specific value.

In two tails, the test sample is checked to be greater or less than a range of values in a Two-Tailed test, implying that the critical distribution area is two-sided.

If the sample falls within this range, the alternate hypothesis will be accepted, and the null hypothesis will be rejected.

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Right Tailed Hypothesis Testing

If the larger than (>) sign appears in your hypothesis statement, you are using a right-tailed test, also known as an upper test. Or, to put it another way, the disparity is to the right. For instance, you can contrast the battery life before and after a change in production. Your hypothesis statements can be the following if you want to know if the battery life is longer than the original (let's say 90 hours):

  • The null hypothesis is (H0 <= 90) or less change.
  • A possibility is that battery life has risen (H1) > 90.

The crucial point in this situation is that the alternate hypothesis (H1), not the null hypothesis, decides whether you get a right-tailed test.

Left Tailed Hypothesis Testing

Alternative hypotheses that assert the true value of a parameter is lower than the null hypothesis are tested with a left-tailed test; they are indicated by the asterisk "<".

Suppose H0: mean = 50 and H1: mean not equal to 50

According to the H1, the mean can be greater than or less than 50. This is an example of a Two-tailed test.

In a similar manner, if H0: mean >=50, then H1: mean <50

Here the mean is less than 50. It is called a One-tailed test.

Type 1 and Type 2 Error

A hypothesis test can result in two types of errors.

Type 1 Error: A Type-I error occurs when sample results reject the null hypothesis despite being true.

Type 2 Error: A Type-II error occurs when the null hypothesis is not rejected when it is false, unlike a Type-I error.

Suppose a teacher evaluates the examination paper to decide whether a student passes or fails.

H0: Student has passed

H1: Student has failed

Type I error will be the teacher failing the student [rejects H0] although the student scored the passing marks [H0 was true]. 

Type II error will be the case where the teacher passes the student [do not reject H0] although the student did not score the passing marks [H1 is true].

Level of Significance

The alpha value is a criterion for determining whether a test statistic is statistically significant. In a statistical test, Alpha represents an acceptable probability of a Type I error. Because alpha is a probability, it can be anywhere between 0 and 1. In practice, the most commonly used alpha values are 0.01, 0.05, and 0.1, which represent a 1%, 5%, and 10% chance of a Type I error, respectively (i.e. rejecting the null hypothesis when it is in fact correct).

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A p-value is a metric that expresses the likelihood that an observed difference could have occurred by chance. As the p-value decreases the statistical significance of the observed difference increases. If the p-value is too low, you reject the null hypothesis.

Here you have taken an example in which you are trying to test whether the new advertising campaign has increased the product's sales. The p-value is the likelihood that the null hypothesis, which states that there is no change in the sales due to the new advertising campaign, is true. If the p-value is .30, then there is a 30% chance that there is no increase or decrease in the product's sales.  If the p-value is 0.03, then there is a 3% probability that there is no increase or decrease in the sales value due to the new advertising campaign. As you can see, the lower the p-value, the chances of the alternate hypothesis being true increases, which means that the new advertising campaign causes an increase or decrease in sales.

Why is Hypothesis Testing Important in Research Methodology?

Hypothesis testing is crucial in research methodology for several reasons:

  • Provides evidence-based conclusions: It allows researchers to make objective conclusions based on empirical data, providing evidence to support or refute their research hypotheses.
  • Supports decision-making: It helps make informed decisions, such as accepting or rejecting a new treatment, implementing policy changes, or adopting new practices.
  • Adds rigor and validity: It adds scientific rigor to research using statistical methods to analyze data, ensuring that conclusions are based on sound statistical evidence.
  • Contributes to the advancement of knowledge: By testing hypotheses, researchers contribute to the growth of knowledge in their respective fields by confirming existing theories or discovering new patterns and relationships.

Limitations of Hypothesis Testing

Hypothesis testing has some limitations that researchers should be aware of:

  • It cannot prove or establish the truth: Hypothesis testing provides evidence to support or reject a hypothesis, but it cannot confirm the absolute truth of the research question.
  • Results are sample-specific: Hypothesis testing is based on analyzing a sample from a population, and the conclusions drawn are specific to that particular sample.
  • Possible errors: During hypothesis testing, there is a chance of committing type I error (rejecting a true null hypothesis) or type II error (failing to reject a false null hypothesis).
  • Assumptions and requirements: Different tests have specific assumptions and requirements that must be met to accurately interpret results.

After reading this tutorial, you would have a much better understanding of hypothesis testing, one of the most important concepts in the field of Data Science . The majority of hypotheses are based on speculation about observed behavior, natural phenomena, or established theories.

If you are interested in statistics of data science and skills needed for such a career, you ought to explore Simplilearn’s Post Graduate Program in Data Science.

If you have any questions regarding this ‘Hypothesis Testing In Statistics’ tutorial, do share them in the comment section. Our subject matter expert will respond to your queries. Happy learning!

1. What is hypothesis testing in statistics with example?

Hypothesis testing is a statistical method used to determine if there is enough evidence in a sample data to draw conclusions about a population. It involves formulating two competing hypotheses, the null hypothesis (H0) and the alternative hypothesis (Ha), and then collecting data to assess the evidence. An example: testing if a new drug improves patient recovery (Ha) compared to the standard treatment (H0) based on collected patient data.

2. What is hypothesis testing and its types?

Hypothesis testing is a statistical method used to make inferences about a population based on sample data. It involves formulating two hypotheses: the null hypothesis (H0), which represents the default assumption, and the alternative hypothesis (Ha), which contradicts H0. The goal is to assess the evidence and determine whether there is enough statistical significance to reject the null hypothesis in favor of the alternative hypothesis.

Types of hypothesis testing:

  • One-sample test: Used to compare a sample to a known value or a hypothesized value.
  • Two-sample test: Compares two independent samples to assess if there is a significant difference between their means or distributions.
  • Paired-sample test: Compares two related samples, such as pre-test and post-test data, to evaluate changes within the same subjects over time or under different conditions.
  • Chi-square test: Used to analyze categorical data and determine if there is a significant association between variables.
  • ANOVA (Analysis of Variance): Compares means across multiple groups to check if there is a significant difference between them.

3. What are the steps of hypothesis testing?

The steps of hypothesis testing are as follows:

  • Formulate the hypotheses: State the null hypothesis (H0) and the alternative hypothesis (Ha) based on the research question.
  • Set the significance level: Determine the acceptable level of error (alpha) for making a decision.
  • Collect and analyze data: Gather and process the sample data.
  • Compute test statistic: Calculate the appropriate statistical test to assess the evidence.
  • Make a decision: Compare the test statistic with critical values or p-values and determine whether to reject H0 in favor of Ha or not.
  • Draw conclusions: Interpret the results and communicate the findings in the context of the research question.

4. What are the 2 types of hypothesis testing?

  • One-tailed (or one-sided) test: Tests for the significance of an effect in only one direction, either positive or negative.
  • Two-tailed (or two-sided) test: Tests for the significance of an effect in both directions, allowing for the possibility of a positive or negative effect.

The choice between one-tailed and two-tailed tests depends on the specific research question and the directionality of the expected effect.

5. What are the 3 major types of hypothesis?

The three major types of hypotheses are:

  • Null Hypothesis (H0): Represents the default assumption, stating that there is no significant effect or relationship in the data.
  • Alternative Hypothesis (Ha): Contradicts the null hypothesis and proposes a specific effect or relationship that researchers want to investigate.
  • Nondirectional Hypothesis: An alternative hypothesis that doesn't specify the direction of the effect, leaving it open for both positive and negative possibilities.

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About the author.

Avijeet Biswal

Avijeet is a Senior Research Analyst at Simplilearn. Passionate about Data Analytics, Machine Learning, and Deep Learning, Avijeet is also interested in politics, cricket, and football.

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8.1: The Elements of Hypothesis Testing

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Learning Objectives

  • To understand the logical framework of tests of hypotheses.
  • To learn basic terminology connected with hypothesis testing.
  • To learn fundamental facts about hypothesis testing.

Types of Hypotheses

A hypothesis about the value of a population parameter is an assertion about its value. As in the introductory example we will be concerned with testing the truth of two competing hypotheses, only one of which can be true.

Definition: null hypothesis and alternative hypothesis

  • The null hypothesis , denoted \(H_0\), is the statement about the population parameter that is assumed to be true unless there is convincing evidence to the contrary.
  • The alternative hypothesis , denoted \(H_a\), is a statement about the population parameter that is contradictory to the null hypothesis, and is accepted as true only if there is convincing evidence in favor of it.

Definition: statistical procedure

Hypothesis testing is a statistical procedure in which a choice is made between a null hypothesis and an alternative hypothesis based on information in a sample.

The end result of a hypotheses testing procedure is a choice of one of the following two possible conclusions:

  • Reject \(H_0\) (and therefore accept \(H_a\)), or
  • Fail to reject \(H_0\) (and therefore fail to accept \(H_a\)).

The null hypothesis typically represents the status quo, or what has historically been true. In the example of the respirators, we would believe the claim of the manufacturer unless there is reason not to do so, so the null hypotheses is \(H_0:\mu =75\). The alternative hypothesis in the example is the contradictory statement \(H_a:\mu <75\). The null hypothesis will always be an assertion containing an equals sign, but depending on the situation the alternative hypothesis can have any one of three forms: with the symbol \(<\), as in the example just discussed, with the symbol \(>\), or with the symbol \(\neq\). The following two examples illustrate the latter two cases.

Example \(\PageIndex{1}\)

A publisher of college textbooks claims that the average price of all hardbound college textbooks is \(\$127.50\). A student group believes that the actual mean is higher and wishes to test their belief. State the relevant null and alternative hypotheses.

The default option is to accept the publisher’s claim unless there is compelling evidence to the contrary. Thus the null hypothesis is \(H_0:\mu =127.50\). Since the student group thinks that the average textbook price is greater than the publisher’s figure, the alternative hypothesis in this situation is \(H_a:\mu >127.50\).

Example \(\PageIndex{2}\)

The recipe for a bakery item is designed to result in a product that contains \(8\) grams of fat per serving. The quality control department samples the product periodically to insure that the production process is working as designed. State the relevant null and alternative hypotheses.

The default option is to assume that the product contains the amount of fat it was formulated to contain unless there is compelling evidence to the contrary. Thus the null hypothesis is \(H_0:\mu =8.0\). Since to contain either more fat than desired or to contain less fat than desired are both an indication of a faulty production process, the alternative hypothesis in this situation is that the mean is different from \(8.0\), so \(H_a:\mu \neq 8.0\).

In Example \(\PageIndex{1}\), the textbook example, it might seem more natural that the publisher’s claim be that the average price is at most \(\$127.50\), not exactly \(\$127.50\). If the claim were made this way, then the null hypothesis would be \(H_0:\mu \leq 127.50\), and the value \(\$127.50\) given in the example would be the one that is least favorable to the publisher’s claim, the null hypothesis. It is always true that if the null hypothesis is retained for its least favorable value, then it is retained for every other value.

Thus in order to make the null and alternative hypotheses easy for the student to distinguish, in every example and problem in this text we will always present one of the two competing claims about the value of a parameter with an equality. The claim expressed with an equality is the null hypothesis. This is the same as always stating the null hypothesis in the least favorable light. So in the introductory example about the respirators, we stated the manufacturer’s claim as “the average is \(75\) minutes” instead of the perhaps more natural “the average is at least \(75\) minutes,” essentially reducing the presentation of the null hypothesis to its worst case.

The first step in hypothesis testing is to identify the null and alternative hypotheses.

The Logic of Hypothesis Testing

Although we will study hypothesis testing in situations other than for a single population mean (for example, for a population proportion instead of a mean or in comparing the means of two different populations), in this section the discussion will always be given in terms of a single population mean \(\mu\).

The null hypothesis always has the form \(H_0:\mu =\mu _0\) for a specific number \(\mu _0\) (in the respirator example \(\mu _0=75\), in the textbook example \(\mu _0=127.50\), and in the baked goods example \(\mu _0=8.0\)). Since the null hypothesis is accepted unless there is strong evidence to the contrary, the test procedure is based on the initial assumption that \(H_0\) is true. This point is so important that we will repeat it in a display:

The test procedure is based on the initial assumption that \(H_0\) is true.

The criterion for judging between \(H_0\) and \(H_a\) based on the sample data is: if the value of \(\overline{X}\) would be highly unlikely to occur if \(H_0\) were true, but favors the truth of \(H_a\), then we reject \(H_0\) in favor of \(H_a\). Otherwise we do not reject \(H_0\).

Supposing for now that \(\overline{X}\) follows a normal distribution, when the null hypothesis is true the density function for the sample mean \(\overline{X}\) must be as in Figure \(\PageIndex{1}\): a bell curve centered at \(\mu _0\). Thus if \(H_0\) is true then \(\overline{X}\) is likely to take a value near \(\mu _0\) and is unlikely to take values far away. Our decision procedure therefore reduces simply to:

  • if \(H_a\) has the form \(H_a:\mu <\mu _0\) then reject \(H_0\) if \(\bar{x}\) is far to the left of \(\mu _0\);
  • if \(H_a\) has the form \(H_a:\mu >\mu _0\) then reject \(H_0\) if \(\bar{x}\) is far to the right of \(\mu _0\);
  • if \(H_a\) has the form \(H_a:\mu \neq \mu _0\) then reject \(H_0\) if \(\bar{x}\) is far away from \(\mu _0\) in either direction.

b91b73d0dbbd53dc069a390a463118a2.jpg

Think of the respirator example, for which the null hypothesis is \(H_0:\mu =75\), the claim that the average time air is delivered for all respirators is \(75\) minutes. If the sample mean is \(75\) or greater then we certainly would not reject \(H_0\) (since there is no issue with an emergency respirator delivering air even longer than claimed).

If the sample mean is slightly less than \(75\) then we would logically attribute the difference to sampling error and also not reject \(H_0\) either.

Values of the sample mean that are smaller and smaller are less and less likely to come from a population for which the population mean is \(75\). Thus if the sample mean is far less than \(75\), say around \(60\) minutes or less, then we would certainly reject \(H_0\), because we know that it is highly unlikely that the average of a sample would be so low if the population mean were \(75\). This is the rare event criterion for rejection: what we actually observed \((\overline{X}<60)\) would be so rare an event if \(\mu =75\) were true that we regard it as much more likely that the alternative hypothesis \(\mu <75\) holds.

In summary, to decide between \(H_0\) and \(H_a\) in this example we would select a “rejection region” of values sufficiently far to the left of \(75\), based on the rare event criterion, and reject \(H_0\) if the sample mean \(\overline{X}\) lies in the rejection region, but not reject \(H_0\) if it does not.

The Rejection Region

Each different form of the alternative hypothesis Ha has its own kind of rejection region:

  • if (as in the respirator example) \(H_a\) has the form \(H_a:\mu <\mu _0\), we reject \(H_0\) if \(\bar{x}\) is far to the left of \(\mu _0\), that is, to the left of some number \(C\), so the rejection region has the form of an interval \((-\infty ,C]\);
  • if (as in the textbook example) \(H_a\) has the form \(H_a:\mu >\mu _0\), we reject \(H_0\) if \(\bar{x}\) is far to the right of \(\mu _0\), that is, to the right of some number \(C\), so the rejection region has the form of an interval \([C,\infty )\);
  • if (as in the baked good example) \(H_a\) has the form \(H_a:\mu \neq \mu _0\), we reject \(H_0\) if \(\bar{x}\) is far away from \(\mu _0\) in either direction, that is, either to the left of some number \(C\) or to the right of some other number \(C′\), so the rejection region has the form of the union of two intervals \((-\infty ,C]\cup [C',\infty )\).

The key issue in our line of reasoning is the question of how to determine the number \(C\) or numbers \(C\) and \(C′\), called the critical value or critical values of the statistic, that determine the rejection region.

Definition: critical values

The critical value or critical values of a test of hypotheses are the number or numbers that determine the rejection region.

Suppose the rejection region is a single interval, so we need to select a single number \(C\). Here is the procedure for doing so. We select a small probability, denoted \(\alpha\), say \(1\%\), which we take as our definition of “rare event:” an event is “rare” if its probability of occurrence is less than \(\alpha\). (In all the examples and problems in this text the value of \(\alpha\) will be given already.) The probability that \(\overline{X}\) takes a value in an interval is the area under its density curve and above that interval, so as shown in Figure \(\PageIndex{2}\) (drawn under the assumption that \(H_0\) is true, so that the curve centers at \(\mu _0\)) the critical value \(C\) is the value of \(\overline{X}\) that cuts off a tail area \(\alpha\) in the probability density curve of \(\overline{X}\). When the rejection region is in two pieces, that is, composed of two intervals, the total area above both of them must be \(\alpha\), so the area above each one is \(\alpha /2\), as also shown in Figure \(\PageIndex{2}\).

72f0cd42fda04cdfb0341bcfe11601c1.jpg

The number \(\alpha\) is the total area of a tail or a pair of tails.

Example \(\PageIndex{3}\)

In the context of Example \(\PageIndex{2}\), suppose that it is known that the population is normally distributed with standard deviation \(\alpha =0.15\) gram, and suppose that the test of hypotheses \(H_0:\mu =8.0\) versus \(H_a:\mu \neq 8.0\) will be performed with a sample of size \(5\). Construct the rejection region for the test for the choice \(\alpha =0.10\). Explain the decision procedure and interpret it.

If \(H_0\) is true then the sample mean \(\overline{X}\) is normally distributed with mean and standard deviation

\[\begin{align} \mu _{\overline{X}} &=\mu \nonumber \\[5pt] &=8.0 \nonumber \end{align} \nonumber \]

\[\begin{align} \sigma _{\overline{X}}&=\dfrac{\sigma}{\sqrt{n}} \nonumber \\[5pt] &= \dfrac{0.15}{\sqrt{5}} \nonumber\\[5pt] &=0.067 \nonumber \end{align} \nonumber \]

Since \(H_a\) contains the \(\neq\) symbol the rejection region will be in two pieces, each one corresponding to a tail of area \(\alpha /2=0.10/2=0.05\). From Figure 7.1.6, \(z_{0.05}=1.645\), so \(C\) and \(C′\) are \(1.645\) standard deviations of \(\overline{X}\) to the right and left of its mean \(8.0\):

\[C=8.0-(1.645)(0.067) = 7.89 \; \; \text{and}\; \; C'=8.0 + (1.645)(0.067) = 8.11 \nonumber \]

The result is shown in Figure \(\PageIndex{3}\). α = 0.1

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The decision procedure is: take a sample of size \(5\) and compute the sample mean \(\bar{x}\). If \(\bar{x}\) is either \(7.89\) grams or less or \(8.11\) grams or more then reject the hypothesis that the average amount of fat in all servings of the product is \(8.0\) grams in favor of the alternative that it is different from \(8.0\) grams. Otherwise do not reject the hypothesis that the average amount is \(8.0\) grams.

The reasoning is that if the true average amount of fat per serving were \(8.0\) grams then there would be less than a \(10\%\) chance that a sample of size \(5\) would produce a mean of either \(7.89\) grams or less or \(8.11\) grams or more. Hence if that happened it would be more likely that the value \(8.0\) is incorrect (always assuming that the population standard deviation is \(0.15\) gram).

Because the rejection regions are computed based on areas in tails of distributions, as shown in Figure \(\PageIndex{2}\), hypothesis tests are classified according to the form of the alternative hypothesis in the following way.

Definitions: Test classifications

  • If \(H_a\) has the form \(\mu \neq \mu _0\) the test is called a two-tailed test .
  • If \(H_a\) has the form \(\mu < \mu _0\) the test is called a left-tailed test .
  • If \(H_a\) has the form \(\mu > \mu _0\)the test is called a right-tailed test .

Each of the last two forms is also called a one-tailed test .

Two Types of Errors

The format of the testing procedure in general terms is to take a sample and use the information it contains to come to a decision about the two hypotheses. As stated before our decision will always be either

  • reject the null hypothesis \(H_0\) in favor of the alternative \(H_a\) presented, or
  • do not reject the null hypothesis \(H_0\) in favor of the alternative \(H_0\) presented.

There are four possible outcomes of hypothesis testing procedure, as shown in the following table:

As the table shows, there are two ways to be right and two ways to be wrong. Typically to reject \(H_0\) when it is actually true is a more serious error than to fail to reject it when it is false, so the former error is labeled “ Type I ” and the latter error “ Type II ”.

Definition: Type I and Type II errors

In a test of hypotheses:

  • A Type I error is the decision to reject \(H_0\) when it is in fact true.
  • A Type II error is the decision not to reject \(H_0\) when it is in fact not true.

Unless we perform a census we do not have certain knowledge, so we do not know whether our decision matches the true state of nature or if we have made an error. We reject \(H_0\) if what we observe would be a “rare” event if \(H_0\) were true. But rare events are not impossible: they occur with probability \(\alpha\). Thus when \(H_0\) is true, a rare event will be observed in the proportion \(\alpha\) of repeated similar tests, and \(H_0\) will be erroneously rejected in those tests. Thus \(\alpha\) is the probability that in following the testing procedure to decide between \(H_0\) and \(H_a\) we will make a Type I error.

Definition: level of significance

The number \(\alpha\) that is used to determine the rejection region is called the level of significance of the test. It is the probability that the test procedure will result in a Type I error .

The probability of making a Type II error is too complicated to discuss in a beginning text, so we will say no more about it than this: for a fixed sample size, choosing \(alpha\) smaller in order to reduce the chance of making a Type I error has the effect of increasing the chance of making a Type II error . The only way to simultaneously reduce the chances of making either kind of error is to increase the sample size.

Standardizing the Test Statistic

Hypotheses testing will be considered in a number of contexts, and great unification as well as simplification results when the relevant sample statistic is standardized by subtracting its mean from it and then dividing by its standard deviation. The resulting statistic is called a standardized test statistic . In every situation treated in this and the following two chapters the standardized test statistic will have either the standard normal distribution or Student’s \(t\)-distribution.

Definition: hypothesis test

A standardized test statistic for a hypothesis test is the statistic that is formed by subtracting from the statistic of interest its mean and dividing by its standard deviation.

For example, reviewing Example \(\PageIndex{3}\), if instead of working with the sample mean \(\overline{X}\) we instead work with the test statistic

\[\frac{\overline{X}-8.0}{0.067} \nonumber \]

then the distribution involved is standard normal and the critical values are just \(\pm z_{0.05}\). The extra work that was done to find that \(C=7.89\) and \(C′=8.11\) is eliminated. In every hypothesis test in this book the standardized test statistic will be governed by either the standard normal distribution or Student’s \(t\)-distribution. Information about rejection regions is summarized in the following tables:

Every instance of hypothesis testing discussed in this and the following two chapters will have a rejection region like one of the six forms tabulated in the tables above.

No matter what the context a test of hypotheses can always be performed by applying the following systematic procedure, which will be illustrated in the examples in the succeeding sections.

Systematic Hypothesis Testing Procedure: Critical Value Approach

  • Identify the null and alternative hypotheses.
  • Identify the relevant test statistic and its distribution.
  • Compute from the data the value of the test statistic.
  • Construct the rejection region.
  • Compare the value computed in Step 3 to the rejection region constructed in Step 4 and make a decision. Formulate the decision in the context of the problem, if applicable.

The procedure that we have outlined in this section is called the “Critical Value Approach” to hypothesis testing to distinguish it from an alternative but equivalent approach that will be introduced at the end of Section 8.3.

Key Takeaway

  • A test of hypotheses is a statistical process for deciding between two competing assertions about a population parameter.
  • The testing procedure is formalized in a five-step procedure.

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What is Hypothesis Testing?

Hypothesis testing in statistics refers to analyzing an assumption about a population parameter. It is used to make an educated guess about an assumption using statistics. With the use of sample data, hypothesis testing makes an assumption about how true the assumption is for the entire population from where the sample is being taken.  

Any hypothetical statement we make may or may not be valid, and it is then our responsibility to provide evidence for its possibility. To approach any hypothesis, we follow these four simple steps that test its validity.

First, we formulate two hypothetical statements such that only one of them is true. By doing so, we can check the validity of our own hypothesis.

The next step is to formulate the statistical analysis to be followed based upon the data points.

Then we analyze the given data using our methodology.

The final step is to analyze the result and judge whether the null hypothesis will be rejected or is true.

Let’s look at several hypothesis testing examples:

It is observed that the average recovery time for a knee-surgery patient is 8 weeks. A physician believes that after successful knee surgery if the patient goes for physical therapy twice a week rather than thrice a week, the recovery period will be longer. Conduct hypothesis for this statement. 

David is a ten-year-old who finishes a 25-yard freestyle in the meantime of 16.43 seconds. David’s father bought goggles for his son, believing that it would help him to reduce his time. He then recorded a total of fifteen 25-yard freestyle for David, and the average time came out to be 16 seconds. Conduct a hypothesis.

A tire company claims their A-segment of tires have a running life of 50,000 miles before they need to be replaced, and previous studies show a standard deviation of 8,000 miles. After surveying a total of 28 tires, the mean run time came to be 46,500 miles with a standard deviation of 9800 miles. Is the claim made by the tire company consistent with the given data? Conduct hypothesis testing. 

All of the hypothesis testing examples are from real-life situations, which leads us to believe that hypothesis testing is a very practical topic indeed. It is an integral part of a researcher's study and is used in every research methodology in one way or another. 

Inferential statistics majorly deals with hypothesis testing. The research hypothesis states there is a relationship between the independent variable and dependent variable. Whereas the null hypothesis rejects this claim of any relationship between the two, our job as researchers or students is to check whether there is any relation between the two.  

Hypothesis Testing in Research Methodology

Now that we are clear about what hypothesis testing is? Let's look at the use of hypothesis testing in research methodology. Hypothesis testing is at the centre of research projects. 

What is Hypothesis Testing and Why is it Important in Research Methodology?

Often after formulating research statements, the validity of those statements need to be verified. Hypothesis testing offers a statistical approach to the researcher about the theoretical assumptions he/she made. It can be understood as quantitative results for a qualitative problem. 

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Hypothesis testing provides various techniques to test the hypothesis statement depending upon the variable and the data points. It finds its use in almost every field of research while answering statements such as whether this new medicine will work, a new testing method is appropriate, or if the outcomes of a random experiment are probable or not.

Procedure of Hypothesis Testing

To find the validity of any statement, we have to strictly follow the stepwise procedure of hypothesis testing. After stating the initial hypothesis, we have to re-write them in the form of a null and alternate hypothesis. The alternate hypothesis predicts a relationship between the variables, whereas the null hypothesis predicts no relationship between the variables.

After writing them as H 0 (null hypothesis) and H a (Alternate hypothesis), only one of the statements can be true. For example, taking the hypothesis that, on average, men are taller than women, we write the statements as:

H 0 : On average, men are not taller than women.

H a : On average, men are taller than women. 

Our next aim is to collect sample data, what we call sampling, in a way so that we can test our hypothesis. Your data should come from the concerned population for which you want to make a hypothesis. 

What is the p value in hypothesis testing? P-value gives us information about the probability of occurrence of results as extreme as observed results.

You will obtain your p-value after choosing the hypothesis testing method, which will be the guiding factor in rejecting the hypothesis. Usually, the p-value cutoff for rejecting the null hypothesis is 0.05. So anything below that, you will reject the null hypothesis. 

A low p-value means that the between-group variance is large enough that there is almost no overlapping, and it is unlikely that these came about by chance. A high p-value suggests there is a high within-group variance and low between-group variance, and any difference in the measure is due to chance only.

What is statistical hypothesis testing?

When forming conclusions through research, two sorts of errors are common: A hypothesis must be set and defined in statistics during a statistical survey or research. A statistical hypothesis is what it is called. It is, in fact, a population parameter assumption. However, it is unmistakable that this idea is always proven correct. Hypothesis testing refers to the predetermined formal procedures used by statisticians to determine whether hypotheses should be accepted or rejected. The process of selecting hypotheses for a given probability distribution based on observable data is known as hypothesis testing. Hypothesis testing is a fundamental and crucial issue in statistics. 

Why do I Need to Test it? Why not just prove an alternate one?

The quick answer is that you must as a scientist; it is part of the scientific process. Science employs a variety of methods to test or reject theories, ensuring that any new hypothesis is free of errors. One protection to ensure your research is not incorrect is to include both a null and an alternate hypothesis. The scientific community considers not incorporating the null hypothesis in your research to be poor practice. You are almost certainly setting yourself up for failure if you set out to prove another theory without first examining it. At the very least, your experiment will not be considered seriously.

Types of Hypothesis Testing

There are several types of hypothesis testing, and they are used based on the data provided. Depending on the sample size and the data given, we choose among different hypothesis testing methodologies. Here starts the use of hypothesis testing tools in research methodology.

Normality- This type of testing is used for normal distribution in a population sample. If the data points are grouped around the mean, the probability of them being above or below the mean is equally likely. Its shape resembles a bell curve that is equally distributed on either side of the mean.

T-test- This test is used when the sample size in a normally distributed population is comparatively small, and the standard deviation is unknown. Usually, if the sample size drops below 30, we use a T-test to find the confidence intervals of the population. 

Chi-Square Test- The Chi-Square test is used to test the population variance against the known or assumed value of the population variance. It is also a better choice to test the goodness of fit of a distribution of data. The two most common Chi-Square tests are the Chi-Square test of independence and the chi-square test of variance.

ANOVA- Analysis of Variance or ANOVA compares the data sets of two different populations or samples. It is similar in its use to the t-test or the Z-test, but it allows us to compare more than two sample means. ANOVA allows us to test the significance between an independent variable and a dependent variable, namely X and Y, respectively.

Z-test- It is a statistical measure to test that the means of two population samples are different when their variance is known. For a Z-test, the population is assumed to be normally distributed. A z-test is better suited in the case of large sample sizes greater than 30. This is due to the central limit theorem that as the sample size increases, the samples are considered to be distributed normally. 

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FAQs on Hypothesis Testing

1. Mention the types of hypothesis Tests.

There are two types of a hypothesis tests:

Null Hypothesis: It is denoted as H₀.

Alternative Hypothesis: IT is denoted as H₁ or Hₐ.

2. What are the two errors that can be found while performing the null Hypothesis test?

While performing the null hypothesis test there is a possibility of occurring two types of errors,

Type-1: The type-1 error is denoted by (α), it is also known as the significance level. It is the rejection of the true null hypothesis. It is the error of commission.

Type-2: The type-2 error is denoted by (β). (1 - β) is known as the power test. The false null hypothesis is not rejected. It is the error of the omission. 

3. What is the p-value in hypothesis testing?

During hypothetical testing in statistics, the p-value indicates the probability of obtaining the result as extreme as observed results. A smaller p-value provides evidence to accept the alternate hypothesis. The p-value is used as a rejection point that provides the smallest level of significance at which the null hypothesis is rejected. Often p-value is calculated using the p-value tables by calculating the deviation between the observed value and the chosen reference value. 

It may also be calculated mathematically by performing integrals on all the values that fall under the curve and areas far from the reference value as the observed value relative to the total area of the curve. The p-value determines the evidence to reject the null hypothesis in hypothesis testing.

4. What is a null hypothesis?

The null hypothesis in statistics says that there is no certain difference between the population. It serves as a conjecture proposing no difference, whereas the alternate hypothesis says there is a difference. When we perform hypothesis testing, we have to state the null hypothesis and alternative hypotheses such that only one of them is ever true. 

By determining the p-value, we calculate whether the null hypothesis is to be rejected or not. If the difference between groups is low, it is merely by chance, and the null hypothesis, which states that there is no difference among groups, is true. Therefore, we have no evidence to reject the null hypothesis.

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Hypothesis testing involves formulating assumptions about population parameters based on sample statistics and rigorously evaluating these assumptions against empirical evidence. This article sheds light on the significance of hypothesis testing and the critical steps involved in the process.

What is Hypothesis Testing?

Hypothesis testing is a statistical method that is used to make a statistical decision using experimental data. Hypothesis testing is basically an assumption that we make about a population parameter. It evaluates two mutually exclusive statements about a population to determine which statement is best supported by the sample data. 

Example: You say an average height in the class is 30 or a boy is taller than a girl. All of these is an assumption that we are assuming, and we need some statistical way to prove these. We need some mathematical conclusion whatever we are assuming is true.

Defining Hypotheses

\mu

Key Terms of Hypothesis Testing

\alpha

  • P-value: The P value , or calculated probability, is the probability of finding the observed/extreme results when the null hypothesis(H0) of a study-given problem is true. If your P-value is less than the chosen significance level then you reject the null hypothesis i.e. accept that your sample claims to support the alternative hypothesis.
  • Test Statistic: The test statistic is a numerical value calculated from sample data during a hypothesis test, used to determine whether to reject the null hypothesis. It is compared to a critical value or p-value to make decisions about the statistical significance of the observed results.
  • Critical value : The critical value in statistics is a threshold or cutoff point used to determine whether to reject the null hypothesis in a hypothesis test.
  • Degrees of freedom: Degrees of freedom are associated with the variability or freedom one has in estimating a parameter. The degrees of freedom are related to the sample size and determine the shape.

Why do we use Hypothesis Testing?

Hypothesis testing is an important procedure in statistics. Hypothesis testing evaluates two mutually exclusive population statements to determine which statement is most supported by sample data. When we say that the findings are statistically significant, thanks to hypothesis testing. 

One-Tailed and Two-Tailed Test

One tailed test focuses on one direction, either greater than or less than a specified value. We use a one-tailed test when there is a clear directional expectation based on prior knowledge or theory. The critical region is located on only one side of the distribution curve. If the sample falls into this critical region, the null hypothesis is rejected in favor of the alternative hypothesis.

One-Tailed Test

There are two types of one-tailed test:

\mu \geq 50

Two-Tailed Test

A two-tailed test considers both directions, greater than and less than a specified value.We use a two-tailed test when there is no specific directional expectation, and want to detect any significant difference.

\mu =

What are Type 1 and Type 2 errors in Hypothesis Testing?

In hypothesis testing, Type I and Type II errors are two possible errors that researchers can make when drawing conclusions about a population based on a sample of data. These errors are associated with the decisions made regarding the null hypothesis and the alternative hypothesis.

\alpha

How does Hypothesis Testing work?

Step 1: define null and alternative hypothesis.

H_0

We first identify the problem about which we want to make an assumption keeping in mind that our assumption should be contradictory to one another, assuming Normally distributed data.

Step 2 – Choose significance level

\alpha

Step 3 – Collect and Analyze data.

Gather relevant data through observation or experimentation. Analyze the data using appropriate statistical methods to obtain a test statistic.

Step 4-Calculate Test Statistic

The data for the tests are evaluated in this step we look for various scores based on the characteristics of data. The choice of the test statistic depends on the type of hypothesis test being conducted.

There are various hypothesis tests, each appropriate for various goal to calculate our test. This could be a Z-test , Chi-square , T-test , and so on.

  • Z-test : If population means and standard deviations are known. Z-statistic is commonly used.
  • t-test : If population standard deviations are unknown. and sample size is small than t-test statistic is more appropriate.
  • Chi-square test : Chi-square test is used for categorical data or for testing independence in contingency tables
  • F-test : F-test is often used in analysis of variance (ANOVA) to compare variances or test the equality of means across multiple groups.

We have a smaller dataset, So, T-test is more appropriate to test our hypothesis.

T-statistic is a measure of the difference between the means of two groups relative to the variability within each group. It is calculated as the difference between the sample means divided by the standard error of the difference. It is also known as the t-value or t-score.

Step 5 – Comparing Test Statistic:

In this stage, we decide where we should accept the null hypothesis or reject the null hypothesis. There are two ways to decide where we should accept or reject the null hypothesis.

Method A: Using Crtical values

Comparing the test statistic and tabulated critical value we have,

  • If Test Statistic>Critical Value: Reject the null hypothesis.
  • If Test Statistic≤Critical Value: Fail to reject the null hypothesis.

Note: Critical values are predetermined threshold values that are used to make a decision in hypothesis testing. To determine critical values for hypothesis testing, we typically refer to a statistical distribution table , such as the normal distribution or t-distribution tables based on.

Method B: Using P-values

We can also come to an conclusion using the p-value,

p\leq\alpha

Note : The p-value is the probability of obtaining a test statistic as extreme as, or more extreme than, the one observed in the sample, assuming the null hypothesis is true. To determine p-value for hypothesis testing, we typically refer to a statistical distribution table , such as the normal distribution or t-distribution tables based on.

Step 7- Interpret the Results

At last, we can conclude our experiment using method A or B.

Calculating test statistic

To validate our hypothesis about a population parameter we use statistical functions . We use the z-score, p-value, and level of significance(alpha) to make evidence for our hypothesis for normally distributed data .

1. Z-statistics:

When population means and standard deviations are known.

z = \frac{\bar{x} - \mu}{\frac{\sigma}{\sqrt{n}}}

  • μ represents the population mean, 
  • σ is the standard deviation
  • and n is the size of the sample.

2. T-Statistics

T test is used when n<30,

t-statistic calculation is given by:

t=\frac{x̄-μ}{s/\sqrt{n}}

  • t = t-score,
  • x̄ = sample mean
  • μ = population mean,
  • s = standard deviation of the sample,
  • n = sample size

3. Chi-Square Test

Chi-Square Test for Independence categorical Data (Non-normally distributed) using:

\chi^2 = \sum \frac{(O_{ij} - E_{ij})^2}{E_{ij}}

  • i,j are the rows and columns index respectively.

E_{ij}

Real life Hypothesis Testing example

Let’s examine hypothesis testing using two real life situations,

Case A: D oes a New Drug Affect Blood Pressure?

Imagine a pharmaceutical company has developed a new drug that they believe can effectively lower blood pressure in patients with hypertension. Before bringing the drug to market, they need to conduct a study to assess its impact on blood pressure.

  • Before Treatment: 120, 122, 118, 130, 125, 128, 115, 121, 123, 119
  • After Treatment: 115, 120, 112, 128, 122, 125, 110, 117, 119, 114

Step 1 : Define the Hypothesis

  • Null Hypothesis : (H 0 )The new drug has no effect on blood pressure.
  • Alternate Hypothesis : (H 1 )The new drug has an effect on blood pressure.

Step 2: Define the Significance level

Let’s consider the Significance level at 0.05, indicating rejection of the null hypothesis.

If the evidence suggests less than a 5% chance of observing the results due to random variation.

Step 3 : Compute the test statistic

Using paired T-test analyze the data to obtain a test statistic and a p-value.

The test statistic (e.g., T-statistic) is calculated based on the differences between blood pressure measurements before and after treatment.

t = m/(s/√n)

  • m  = mean of the difference i.e X after, X before
  • s  = standard deviation of the difference (d) i.e d i ​= X after, i ​− X before,
  • n  = sample size,

then, m= -3.9, s= 1.8 and n= 10

we, calculate the , T-statistic = -9 based on the formula for paired t test

Step 4: Find the p-value

The calculated t-statistic is -9 and degrees of freedom df = 9, you can find the p-value using statistical software or a t-distribution table.

thus, p-value = 8.538051223166285e-06

Step 5: Result

  • If the p-value is less than or equal to 0.05, the researchers reject the null hypothesis.
  • If the p-value is greater than 0.05, they fail to reject the null hypothesis.

Conclusion: Since the p-value (8.538051223166285e-06) is less than the significance level (0.05), the researchers reject the null hypothesis. There is statistically significant evidence that the average blood pressure before and after treatment with the new drug is different.

Python Implementation of Hypothesis Testing

Let’s create hypothesis testing with python, where we are testing whether a new drug affects blood pressure. For this example, we will use a paired T-test. We’ll use the scipy.stats library for the T-test.

Scipy is a mathematical library in Python that is mostly used for mathematical equations and computations.

We will implement our first real life problem via python,

In the above example, given the T-statistic of approximately -9 and an extremely small p-value, the results indicate a strong case to reject the null hypothesis at a significance level of 0.05. 

  • The results suggest that the new drug, treatment, or intervention has a significant effect on lowering blood pressure.
  • The negative T-statistic indicates that the mean blood pressure after treatment is significantly lower than the assumed population mean before treatment.

Case B : Cholesterol level in a population

Data: A sample of 25 individuals is taken, and their cholesterol levels are measured.

Cholesterol Levels (mg/dL): 205, 198, 210, 190, 215, 205, 200, 192, 198, 205, 198, 202, 208, 200, 205, 198, 205, 210, 192, 205, 198, 205, 210, 192, 205.

Populations Mean = 200

Population Standard Deviation (σ): 5 mg/dL(given for this problem)

Step 1: Define the Hypothesis

  • Null Hypothesis (H 0 ): The average cholesterol level in a population is 200 mg/dL.
  • Alternate Hypothesis (H 1 ): The average cholesterol level in a population is different from 200 mg/dL.

As the direction of deviation is not given , we assume a two-tailed test, and based on a normal distribution table, the critical values for a significance level of 0.05 (two-tailed) can be calculated through the z-table and are approximately -1.96 and 1.96.

(203.8 - 200) / (5 \div \sqrt{25})

Step 4: Result

Since the absolute value of the test statistic (2.04) is greater than the critical value (1.96), we reject the null hypothesis. And conclude that, there is statistically significant evidence that the average cholesterol level in the population is different from 200 mg/dL

Limitations of Hypothesis Testing

  • Although a useful technique, hypothesis testing does not offer a comprehensive grasp of the topic being studied. Without fully reflecting the intricacy or whole context of the phenomena, it concentrates on certain hypotheses and statistical significance.
  • The accuracy of hypothesis testing results is contingent on the quality of available data and the appropriateness of statistical methods used. Inaccurate data or poorly formulated hypotheses can lead to incorrect conclusions.
  • Relying solely on hypothesis testing may cause analysts to overlook significant patterns or relationships in the data that are not captured by the specific hypotheses being tested. This limitation underscores the importance of complimenting hypothesis testing with other analytical approaches.

Hypothesis testing stands as a cornerstone in statistical analysis, enabling data scientists to navigate uncertainties and draw credible inferences from sample data. By systematically defining null and alternative hypotheses, choosing significance levels, and leveraging statistical tests, researchers can assess the validity of their assumptions. The article also elucidates the critical distinction between Type I and Type II errors, providing a comprehensive understanding of the nuanced decision-making process inherent in hypothesis testing. The real-life example of testing a new drug’s effect on blood pressure using a paired T-test showcases the practical application of these principles, underscoring the importance of statistical rigor in data-driven decision-making.

Frequently Asked Questions (FAQs)

1. what are the 3 types of hypothesis test.

There are three types of hypothesis tests: right-tailed, left-tailed, and two-tailed. Right-tailed tests assess if a parameter is greater, left-tailed if lesser. Two-tailed tests check for non-directional differences, greater or lesser.

2.What are the 4 components of hypothesis testing?

Null Hypothesis ( ): No effect or difference exists. Alternative Hypothesis ( ): An effect or difference exists. Significance Level ( ): Risk of rejecting null hypothesis when it’s true (Type I error). Test Statistic: Numerical value representing observed evidence against null hypothesis.

3.What is hypothesis testing in ML?

Statistical method to evaluate the performance and validity of machine learning models. Tests specific hypotheses about model behavior, like whether features influence predictions or if a model generalizes well to unseen data.

4.What is the difference between Pytest and hypothesis in Python?

Pytest purposes general testing framework for Python code while Hypothesis is a Property-based testing framework for Python, focusing on generating test cases based on specified properties of the code.

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Title: multiple object tracking as id prediction.

Abstract: In Multiple Object Tracking (MOT), tracking-by-detection methods have stood the test for a long time, which split the process into two parts according to the definition: object detection and association. They leverage robust single-frame detectors and treat object association as a post-processing step through hand-crafted heuristic algorithms and surrogate tasks. However, the nature of heuristic techniques prevents end-to-end exploitation of training data, leading to increasingly cumbersome and challenging manual modification while facing complicated or novel scenarios. In this paper, we regard this object association task as an End-to-End in-context ID prediction problem and propose a streamlined baseline called MOTIP. Specifically, we form the target embeddings into historical trajectory information while considering the corresponding IDs as in-context prompts, then directly predict the ID labels for the objects in the current frame. Thanks to this end-to-end process, MOTIP can learn tracking capabilities straight from training data, freeing itself from burdensome hand-crafted algorithms. Without bells and whistles, our method achieves impressive state-of-the-art performance in complex scenarios like DanceTrack and SportsMOT, and it performs competitively with other transformer-based methods on MOT17. We believe that MOTIP demonstrates remarkable potential and can serve as a starting point for future research. The code is available at this https URL .

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  1. Hypothesis Testing

    Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is most often used by scientists to test specific predictions, called hypotheses, that arise from theories. Learn the 5 main steps of hypothesis testing, from state your null and alternate hypothesis to present your results and discussion.

  2. Introduction to Hypothesis Testing

    A statistical hypothesis is an assumption about a population parameter. A hypothesis test is a formal statistical test we use to reject or fail to reject a statistical hypothesis. Learn the two types of statistical hypotheses, the five steps of a hypothesis test, the two types of decision errors, and the two types of hypothesis tests.

  3. Statistical hypothesis test

    A statistical hypothesis test is a method of statistical inference used to decide whether the data sufficiently support a particular hypothesis. A statistical hypothesis test typically involves a calculation of a test statistic. Then a decision is made, either by comparing the test statistic to a critical value or equivalently by evaluating a p ...

  4. Hypothesis to Be Tested: Definition and 4 Steps for Testing with Example

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    Hypothesis testing. Hypothesis testing is a form of statistical inference that uses data from a sample to draw conclusions about a population parameter or a population probability distribution.First, a tentative assumption is made about the parameter or distribution. This assumption is called the null hypothesis and is denoted by H 0.An alternative hypothesis (denoted H a), which is the ...

  6. 9.1: Introduction to Hypothesis Testing

    In hypothesis testing, the goal is to see if there is sufficient statistical evidence to reject a presumed null hypothesis in favor of a conjectured alternative hypothesis.The null hypothesis is usually denoted \(H_0\) while the alternative hypothesis is usually denoted \(H_1\). An hypothesis test is a statistical decision; the conclusion will either be to reject the null hypothesis in favor ...

  7. 3.1: The Fundamentals of Hypothesis Testing

    A hypothesis is a claim or statement about a characteristic of a population of interest to us. A hypothesis test is a way for us to use our sample statistics to test a specific claim. Example 3.1.1: The population mean weight is known to be 157 lb. We want to test the claim that the mean weight has increased.

  8. S.3 Hypothesis Testing

    hypothesis testing. S.3 Hypothesis Testing. In reviewing hypothesis tests, we start first with the general idea. Then, we keep returning to the basic procedures of hypothesis testing, each time adding a little more detail. The general idea of hypothesis testing involves: Making an initial assumption. Collecting evidence (data).

  9. Hypothesis testing

    hypothesis testing, In statistics, a method for testing how accurately a mathematical model based on one set of data predicts the nature of other data sets generated by the same process. Hypothesis testing grew out of quality control, in which whole batches of manufactured items are accepted or rejected based on testing relatively small samples.An initial hypothesis (null hypothesis) might ...

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  11. Statistical Hypothesis Testing Overview

    Hypothesis testing is a crucial procedure to perform when you want to make inferences about a population using a random sample. These inferences include estimating population properties such as the mean, differences between means, proportions, and the relationships between variables. This post provides an overview of statistical hypothesis testing.

  12. 7.1: Basics of Hypothesis Testing

    Test Statistic: z = ¯ x − μo σ / √n since it is calculated as part of the testing of the hypothesis. Definition 7.1.4. p - value: probability that the test statistic will take on more extreme values than the observed test statistic, given that the null hypothesis is true.

  13. Hypothesis Testing

    Hypothesis testing is a technique that is used to verify whether the results of an experiment are statistically significant. It involves the setting up of a null hypothesis and an alternate hypothesis. There are three types of tests that can be conducted under hypothesis testing - z test, t test, and chi square test.

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    HYPOTHESIS TESTING. A clinical trial begins with an assumption or belief, and then proceeds to either prove or disprove this assumption. In statistical terms, this belief or assumption is known as a hypothesis. Counterintuitively, what the researcher believes in (or is trying to prove) is called the "alternate" hypothesis, and the opposite ...

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    Start Unit test. Significance tests give us a formal process for using sample data to evaluate the likelihood of some claim about a population value. Learn how to conduct significance tests and calculate p-values to see how likely a sample result is to occur by random chance. You'll also see how we use p-values to make conclusions about hypotheses.

  17. Hypothesis Testing: Definition, Uses, Limitations + Examples

    Hypothesis testing is a research method that allows researchers to test an assumption about a population parameter based on limited evidence. It involves creating a null and alternative hypothesis, setting a significance level, and using test statistics to compare the association or relationship between variables. Learn the types, stages, and examples of hypothesis testing.

  18. What is Hypothesis Testing in Statistics? Types and Examples

    Hypothesis testing is a statistical method used to determine if there is enough evidence in a sample data to draw conclusions about a population. It involves formulating two competing hypotheses, the null hypothesis (H0) and the alternative hypothesis (Ha), and then collecting data to assess the evidence.

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    Definition: statistical procedure. Hypothesis testing is a statistical procedure in which a choice is made between a null hypothesis and an alternative hypothesis based on information in a sample. The end result of a hypotheses testing procedure is a choice of one of the following two possible conclusions: Reject H0.

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  24. [2403.16848] Multiple Object Tracking as ID Prediction

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