Lock-and-key model

Lock-and-key model definition example

strong>Lock-and-key model n., [lɑk ænd ki ˈmɑdl̩] Definition: a model for enzyme-substrate interaction

Table of Contents

Lock-and-key model Definition

Lock-and-key model is a model for enzyme-substrate interaction suggesting that the enzyme and the substrate possess specific complementary geometric shapes that fit exactly into one another. In this model, enzymes are depicted as highly specific. They must bind to specific substrates before they catalyze chemical reactions . The term is a pivotal concept in enzymology to elucidate the intricate interaction between enzymes and substrates at the molecular level. In the lock-and-key model, the enzyme-substrate interaction suggests that the enzyme and the substrate possess specific complementary geometric shapes that fit exactly into one another. Like a key  into a  lock , only the correct size and shape of the substrate ( the key ) would fit into the  active site  ( the keyhole ) of the enzyme ( the lock ).

Compare: Induced fit model   See also: enzyme , active site , substrate

Lock-and-key vs. Induced Fit Model

At present, two models attempt to explain enzyme-substrate specificity; one of which is the lock-and-key model , and the other is the Induced fit model . The lock and key model theory was first postulated by  Emil Fischer   in 1894. The lock-and-key enzyme action proposes the high specificity of enzymes. However, it does not explain the stabilization of the transition state that the enzymes achieve. The induced fit model (proposed by Daniel Koshland in 1958) suggests that the active site continues to change until the substrate is completely bound to the active site of the enzyme, at which point the final shape and charge are determined. Unlike the lock-and-key model, the induced fit model shows that enzymes are rather flexible structures. Nevertheless, Fischer’s Lock and Key theory laid an important foundation for subsequent research, such as during the refinement of the enzyme-substrate complex mechanism, as ascribed in the induced fit model. The lock-and-key hypothesis has opened ideas where enzyme action is not merely catalytic but incorporates a rather complex process in how they interact with the correct substrates with precision.

Lock and key model definition and example

Key Components

Components of the lock and key model:

  • Enzyme : the enzyme structure is a three-dimensional protein configuration, with an active site from where the substrate binds.
  • Substrate : often an organic molecule, a substrate possesses a structural feature that complements the geometry of the enzyme’s active site.

In the lock and key model, both the enzymes and the substrates facilitate the formation of a complex that lowers the activation energy needed for a chemical transformation to occur. Such reduction in the activation energy allows the chemical reaction to proceed at a relatively faster rate, making enzymes crucial in various biological and molecular processes.

Lock-and-key Model Examples

Some of the common examples that are often discussed in the context of the Lock and Key Model are as follows:

  • Enzyme lactate dehydrogenase with a specific active site for its substrates, pyruvate and lactate. The complex facilitates the interconversion of pyruvate and lactate during anaerobic respiration
  • Enzyme carbonic anhydrase with a specific active site for the substrates carbon dioxide and water. The complex facilitates the hydration of carbon dioxide, forming bicarbonate
  • Enzyme lysozyme binding with a bacterial cell wall peptidoglycan, which is a vital immune function

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  • Aryal, S. and Karki, P. (2023).  “Lock and Key Model- Mode of Action of Enzymes”. Microbenotes.com. https://microbenotes.com/lock-and-key-model-mode-of-action-of-enzymes/
  • Farhana, A., & Lappin, S. L. (2023, May).  Biochemistry, Lactate Dehydrogenase . Nih.gov; StatPearls Publishing. https://www.ncbi.nlm.nih.gov/books/NBK557536/

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Biology library

Course: biology library   >   unit 1, the scientific method.

  • Controlled experiments
  • The scientific method and experimental design

Introduction

  • Make an observation.
  • Ask a question.
  • Form a hypothesis , or testable explanation.
  • Make a prediction based on the hypothesis.
  • Test the prediction.
  • Iterate: use the results to make new hypotheses or predictions.

Scientific method example: Failure to toast

1. make an observation..

  • Observation: the toaster won't toast.

2. Ask a question.

  • Question: Why won't my toaster toast?

3. Propose a hypothesis.

  • Hypothesis: Maybe the outlet is broken.

4. Make predictions.

  • Prediction: If I plug the toaster into a different outlet, then it will toast the bread.

5. Test the predictions.

  • Test of prediction: Plug the toaster into a different outlet and try again.
  • If the toaster does toast, then the hypothesis is supported—likely correct.
  • If the toaster doesn't toast, then the hypothesis is not supported—likely wrong.

Logical possibility

Practical possibility, building a body of evidence, 6. iterate..

  • Iteration time!
  • If the hypothesis was supported, we might do additional tests to confirm it, or revise it to be more specific. For instance, we might investigate why the outlet is broken.
  • If the hypothesis was not supported, we would come up with a new hypothesis. For instance, the next hypothesis might be that there's a broken wire in the toaster.

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What Is A Research (Scientific) Hypothesis? A plain-language explainer + examples

By:  Derek Jansen (MBA)  | Reviewed By: Dr Eunice Rautenbach | June 2020

If you’re new to the world of research, or it’s your first time writing a dissertation or thesis, you’re probably noticing that the words “research hypothesis” and “scientific hypothesis” are used quite a bit, and you’re wondering what they mean in a research context .

“Hypothesis” is one of those words that people use loosely, thinking they understand what it means. However, it has a very specific meaning within academic research. So, it’s important to understand the exact meaning before you start hypothesizing. 

Research Hypothesis 101

  • What is a hypothesis ?
  • What is a research hypothesis (scientific hypothesis)?
  • Requirements for a research hypothesis
  • Definition of a research hypothesis
  • The null hypothesis

What is a hypothesis?

Let’s start with the general definition of a hypothesis (not a research hypothesis or scientific hypothesis), according to the Cambridge Dictionary:

Hypothesis: an idea or explanation for something that is based on known facts but has not yet been proved.

In other words, it’s a statement that provides an explanation for why or how something works, based on facts (or some reasonable assumptions), but that has not yet been specifically tested . For example, a hypothesis might look something like this:

Hypothesis: sleep impacts academic performance.

This statement predicts that academic performance will be influenced by the amount and/or quality of sleep a student engages in – sounds reasonable, right? It’s based on reasonable assumptions , underpinned by what we currently know about sleep and health (from the existing literature). So, loosely speaking, we could call it a hypothesis, at least by the dictionary definition.

But that’s not good enough…

Unfortunately, that’s not quite sophisticated enough to describe a research hypothesis (also sometimes called a scientific hypothesis), and it wouldn’t be acceptable in a dissertation, thesis or research paper . In the world of academic research, a statement needs a few more criteria to constitute a true research hypothesis .

What is a research hypothesis?

A research hypothesis (also called a scientific hypothesis) is a statement about the expected outcome of a study (for example, a dissertation or thesis). To constitute a quality hypothesis, the statement needs to have three attributes – specificity , clarity and testability .

Let’s take a look at these more closely.

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key hypothesis meaning

Hypothesis Essential #1: Specificity & Clarity

A good research hypothesis needs to be extremely clear and articulate about both what’ s being assessed (who or what variables are involved ) and the expected outcome (for example, a difference between groups, a relationship between variables, etc.).

Let’s stick with our sleepy students example and look at how this statement could be more specific and clear.

Hypothesis: Students who sleep at least 8 hours per night will, on average, achieve higher grades in standardised tests than students who sleep less than 8 hours a night.

As you can see, the statement is very specific as it identifies the variables involved (sleep hours and test grades), the parties involved (two groups of students), as well as the predicted relationship type (a positive relationship). There’s no ambiguity or uncertainty about who or what is involved in the statement, and the expected outcome is clear.

Contrast that to the original hypothesis we looked at – “Sleep impacts academic performance” – and you can see the difference. “Sleep” and “academic performance” are both comparatively vague , and there’s no indication of what the expected relationship direction is (more sleep or less sleep). As you can see, specificity and clarity are key.

A good research hypothesis needs to be very clear about what’s being assessed and very specific about the expected outcome.

Hypothesis Essential #2: Testability (Provability)

A statement must be testable to qualify as a research hypothesis. In other words, there needs to be a way to prove (or disprove) the statement. If it’s not testable, it’s not a hypothesis – simple as that.

For example, consider the hypothesis we mentioned earlier:

Hypothesis: Students who sleep at least 8 hours per night will, on average, achieve higher grades in standardised tests than students who sleep less than 8 hours a night.  

We could test this statement by undertaking a quantitative study involving two groups of students, one that gets 8 or more hours of sleep per night for a fixed period, and one that gets less. We could then compare the standardised test results for both groups to see if there’s a statistically significant difference. 

Again, if you compare this to the original hypothesis we looked at – “Sleep impacts academic performance” – you can see that it would be quite difficult to test that statement, primarily because it isn’t specific enough. How much sleep? By who? What type of academic performance?

So, remember the mantra – if you can’t test it, it’s not a hypothesis 🙂

A good research hypothesis must be testable. In other words, you must able to collect observable data in a scientifically rigorous fashion to test it.

Defining A Research Hypothesis

You’re still with us? Great! Let’s recap and pin down a clear definition of a hypothesis.

A research hypothesis (or scientific hypothesis) is a statement about an expected relationship between variables, or explanation of an occurrence, that is clear, specific and testable.

So, when you write up hypotheses for your dissertation or thesis, make sure that they meet all these criteria. If you do, you’ll not only have rock-solid hypotheses but you’ll also ensure a clear focus for your entire research project.

What about the null hypothesis?

You may have also heard the terms null hypothesis , alternative hypothesis, or H-zero thrown around. At a simple level, the null hypothesis is the counter-proposal to the original hypothesis.

For example, if the hypothesis predicts that there is a relationship between two variables (for example, sleep and academic performance), the null hypothesis would predict that there is no relationship between those variables.

At a more technical level, the null hypothesis proposes that no statistical significance exists in a set of given observations and that any differences are due to chance alone.

And there you have it – hypotheses in a nutshell. 

If you have any questions, be sure to leave a comment below and we’ll do our best to help you. If you need hands-on help developing and testing your hypotheses, consider our private coaching service , where we hold your hand through the research journey.

key hypothesis meaning

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This post was based on one of our popular Research Bootcamps . If you're working on a research project, you'll definitely want to check this out ...

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16 Comments

Lynnet Chikwaikwai

Very useful information. I benefit more from getting more information in this regard.

Dr. WuodArek

Very great insight,educative and informative. Please give meet deep critics on many research data of public international Law like human rights, environment, natural resources, law of the sea etc

Afshin

In a book I read a distinction is made between null, research, and alternative hypothesis. As far as I understand, alternative and research hypotheses are the same. Can you please elaborate? Best Afshin

GANDI Benjamin

This is a self explanatory, easy going site. I will recommend this to my friends and colleagues.

Lucile Dossou-Yovo

Very good definition. How can I cite your definition in my thesis? Thank you. Is nul hypothesis compulsory in a research?

Pereria

It’s a counter-proposal to be proven as a rejection

Egya Salihu

Please what is the difference between alternate hypothesis and research hypothesis?

Mulugeta Tefera

It is a very good explanation. However, it limits hypotheses to statistically tasteable ideas. What about for qualitative researches or other researches that involve quantitative data that don’t need statistical tests?

Derek Jansen

In qualitative research, one typically uses propositions, not hypotheses.

Samia

could you please elaborate it more

Patricia Nyawir

I’ve benefited greatly from these notes, thank you.

Hopeson Khondiwa

This is very helpful

Dr. Andarge

well articulated ideas are presented here, thank you for being reliable sources of information

TAUNO

Excellent. Thanks for being clear and sound about the research methodology and hypothesis (quantitative research)

I have only a simple question regarding the null hypothesis. – Is the null hypothesis (Ho) known as the reversible hypothesis of the alternative hypothesis (H1? – How to test it in academic research?

Tesfaye Negesa Urge

this is very important note help me much more

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Molecular Recognition: Lock-and-Key, Induced Fit, and Conformational Selection

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key hypothesis meaning

  • Norman Tran 4 &
  • Todd Holyoak 4  

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In the most general sense, molecular recognition is the mechanism by which two or more molecules come together to form a specific complex. These types of molecular interactions are widespread throughout biology and include diverse processes such as enzyme catalysis, antibody–antigen recognition, protein synthesis, receptor–ligand interactions, and transcriptional regulation, to name a few. Because of the universal importance of molecular recognition in biological function, understanding how molecules unambiguously recognize and interact with one another is fundamentally important to appreciating biological systems as a whole.

Introduction

Just as the field biochemistry grew out of the study of biological fermentation, much of the field of molecular recognition grew out of the study of enzyme selectivity (Voet and Voet 2004 ). Early studies led to the conclusion that substrates combine with enzymes at a specific location on each enzyme’s surface. These conclusions generated...

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Voet D, Voet JG (2004) Biochemistry. Wiley, Hoboken

Weikl TR, Paul F (2014) Conformational selection in protein binding and function. Protein Sci 23(11):1508–1518

Yang J, Gao M, Xiong JW, Su ZD, Huang YQ (2019) Features of molecular recognition of intrinsically disordered proteins via coupled folding and binding. Protein Sci 28(11):1952–1965

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Tran, N., Holyoak, T. (2021). Molecular Recognition: Lock-and-Key, Induced Fit, and Conformational Selection. In: Roberts, G., Watts, A. (eds) Encyclopedia of Biophysics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-35943-9_468-1

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  • Biological Molecules

Enzymes are Biological Catalysts . They increase the rate of Metabolic reactions . Almost all Biological Reactions involve Enzymes. All enzymes are Globular Proteins with a specific Tertiary Shape . They are usually specific to only one reaction.

The part of the Enzyme that acts a Catalyst is called the Active Site . The rest of the Enzyme is much larger and is involved in maintaining the specific shape of of the Enzyme.

When a reaction involving an Enzyme occurs, a Substrate is turned into a Product . The Substrate can be one or more molecules. The Active Site of an Enzyme is Complementary to the Substrate it catalyses.

  • Lactase : Breaks down Lactose into Glucose and Galactose.
  • Catalase : Breaks Hydrogen Peroxide down into Water and Oxygen.
  • Glycogen Synthase : Catalyses the formation of Glycosidic Bonds between Glucose molecules.
  • ATP-ase : Breaks down ATP into ADP, producing energy._

Enzymes in the Real World

Since Enzymes are Proteins, which are effected by their environment , organisms that live in varying conditions have adapted by producing Enzymes more suitable to their environments. Endotherms (animals that maintain their body temperature) keep the temperature of the Enzymes within their bodies constant to ensure optimum rates of reaction .

Enzymes are used for a wide variety of purposes, such as in digestion . The action of an Enzyme may be Intracellular (the Enzymes are attached to the cell membrane or are in the Cytoplasm , and reactions occur inside the cell) or Extracellular (Enzymes work outside cells , and their products may be absorbed into the cell)

Enzymes are also used in protection against Pathogens . They can be used to destroy invading Microorgansims. For example, Phagocytes engulf Pathogens and the Endocytosed Vesicle then fuses with Lysosomes which contain enzymes that destroy the Pathogen’s cell membrane.

How Enzymes Work

  • Most reactions in a cell require very high temperatures to get going, which would destroy the cell. Enzymes work by lowering the Activation Energy of a reaction.
  • The Activation Energy of a reaction is lowered by putting stress on the bonds within a molecule, or by holding molecules close together. This increases the likelihood of a reaction, and so lowers the energy required to begin it.

The Lock-and-key Hypothesis

The Lock-and-key Hypothesis is a model of how Enzymes catalyse Substrate reactions. It states that the shape of the Active Sites of Enzymes are exactly Complementary to the shape of the Substrate.

When a substrate molecule collides with an enzyme whose Active Site shape is complementary, the substrate will fit into the Active Site and an Enzyme-Substrate Complex will form.

  • The enzyme will catalyse the reaction, and the products , together with the enzyme, will form an Enzyme-Product Complex . According to this model, it is possible for an enzyme to catalyse a reverse reaction.

key hypothesis meaning

The Induced-Fit Hypothesis

A more recent model, which is backed up by evidence ,and is widely accepted as describing the way enzymes work, is the Induced-Fit Hypothesis . It states that the shape of Active Sites are not exactly Complementary , but change shape in the presence of a specific substrate to become Complementary .

When a substrate molecule collides with an enzyme, if its composition is specifically correct, the shape of the enzyme’s Active Site will change so that the substrate fits into it and an Enzyme-Substrate Complex can form. The reaction is then catalysed and an Enzyme-Product Complex forms.

key hypothesis meaning

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A theory to explain the mechanism of enzymatic reactions, in which it is proposed that the enzyme and substrate(s) bind temporarily to form an enzyme–substrate complex. The binding site on the enzyme is known as the ‘active site’ and is structurally complementary to the substrate(s). Thus the enzyme and substrate(s) are said to fit together as do a lock and a key.

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Research Method

Home » What is a Hypothesis – Types, Examples and Writing Guide

What is a Hypothesis – Types, Examples and Writing Guide

Table of Contents

What is a Hypothesis

Definition:

Hypothesis is an educated guess or proposed explanation for a phenomenon, based on some initial observations or data. It is a tentative statement that can be tested and potentially proven or disproven through further investigation and experimentation.

Hypothesis is often used in scientific research to guide the design of experiments and the collection and analysis of data. It is an essential element of the scientific method, as it allows researchers to make predictions about the outcome of their experiments and to test those predictions to determine their accuracy.

Types of Hypothesis

Types of Hypothesis are as follows:

Research Hypothesis

A research hypothesis is a statement that predicts a relationship between variables. It is usually formulated as a specific statement that can be tested through research, and it is often used in scientific research to guide the design of experiments.

Null Hypothesis

The null hypothesis is a statement that assumes there is no significant difference or relationship between variables. It is often used as a starting point for testing the research hypothesis, and if the results of the study reject the null hypothesis, it suggests that there is a significant difference or relationship between variables.

Alternative Hypothesis

An alternative hypothesis is a statement that assumes there is a significant difference or relationship between variables. It is often used as an alternative to the null hypothesis and is tested against the null hypothesis to determine which statement is more accurate.

Directional Hypothesis

A directional hypothesis is a statement that predicts the direction of the relationship between variables. For example, a researcher might predict that increasing the amount of exercise will result in a decrease in body weight.

Non-directional Hypothesis

A non-directional hypothesis is a statement that predicts the relationship between variables but does not specify the direction. For example, a researcher might predict that there is a relationship between the amount of exercise and body weight, but they do not specify whether increasing or decreasing exercise will affect body weight.

Statistical Hypothesis

A statistical hypothesis is a statement that assumes a particular statistical model or distribution for the data. It is often used in statistical analysis to test the significance of a particular result.

Composite Hypothesis

A composite hypothesis is a statement that assumes more than one condition or outcome. It can be divided into several sub-hypotheses, each of which represents a different possible outcome.

Empirical Hypothesis

An empirical hypothesis is a statement that is based on observed phenomena or data. It is often used in scientific research to develop theories or models that explain the observed phenomena.

Simple Hypothesis

A simple hypothesis is a statement that assumes only one outcome or condition. It is often used in scientific research to test a single variable or factor.

Complex Hypothesis

A complex hypothesis is a statement that assumes multiple outcomes or conditions. It is often used in scientific research to test the effects of multiple variables or factors on a particular outcome.

Applications of Hypothesis

Hypotheses are used in various fields to guide research and make predictions about the outcomes of experiments or observations. Here are some examples of how hypotheses are applied in different fields:

  • Science : In scientific research, hypotheses are used to test the validity of theories and models that explain natural phenomena. For example, a hypothesis might be formulated to test the effects of a particular variable on a natural system, such as the effects of climate change on an ecosystem.
  • Medicine : In medical research, hypotheses are used to test the effectiveness of treatments and therapies for specific conditions. For example, a hypothesis might be formulated to test the effects of a new drug on a particular disease.
  • Psychology : In psychology, hypotheses are used to test theories and models of human behavior and cognition. For example, a hypothesis might be formulated to test the effects of a particular stimulus on the brain or behavior.
  • Sociology : In sociology, hypotheses are used to test theories and models of social phenomena, such as the effects of social structures or institutions on human behavior. For example, a hypothesis might be formulated to test the effects of income inequality on crime rates.
  • Business : In business research, hypotheses are used to test the validity of theories and models that explain business phenomena, such as consumer behavior or market trends. For example, a hypothesis might be formulated to test the effects of a new marketing campaign on consumer buying behavior.
  • Engineering : In engineering, hypotheses are used to test the effectiveness of new technologies or designs. For example, a hypothesis might be formulated to test the efficiency of a new solar panel design.

How to write a Hypothesis

Here are the steps to follow when writing a hypothesis:

Identify the Research Question

The first step is to identify the research question that you want to answer through your study. This question should be clear, specific, and focused. It should be something that can be investigated empirically and that has some relevance or significance in the field.

Conduct a Literature Review

Before writing your hypothesis, it’s essential to conduct a thorough literature review to understand what is already known about the topic. This will help you to identify the research gap and formulate a hypothesis that builds on existing knowledge.

Determine the Variables

The next step is to identify the variables involved in the research question. A variable is any characteristic or factor that can vary or change. There are two types of variables: independent and dependent. The independent variable is the one that is manipulated or changed by the researcher, while the dependent variable is the one that is measured or observed as a result of the independent variable.

Formulate the Hypothesis

Based on the research question and the variables involved, you can now formulate your hypothesis. A hypothesis should be a clear and concise statement that predicts the relationship between the variables. It should be testable through empirical research and based on existing theory or evidence.

Write the Null Hypothesis

The null hypothesis is the opposite of the alternative hypothesis, which is the hypothesis that you are testing. The null hypothesis states that there is no significant difference or relationship between the variables. It is important to write the null hypothesis because it allows you to compare your results with what would be expected by chance.

Refine the Hypothesis

After formulating the hypothesis, it’s important to refine it and make it more precise. This may involve clarifying the variables, specifying the direction of the relationship, or making the hypothesis more testable.

Examples of Hypothesis

Here are a few examples of hypotheses in different fields:

  • Psychology : “Increased exposure to violent video games leads to increased aggressive behavior in adolescents.”
  • Biology : “Higher levels of carbon dioxide in the atmosphere will lead to increased plant growth.”
  • Sociology : “Individuals who grow up in households with higher socioeconomic status will have higher levels of education and income as adults.”
  • Education : “Implementing a new teaching method will result in higher student achievement scores.”
  • Marketing : “Customers who receive a personalized email will be more likely to make a purchase than those who receive a generic email.”
  • Physics : “An increase in temperature will cause an increase in the volume of a gas, assuming all other variables remain constant.”
  • Medicine : “Consuming a diet high in saturated fats will increase the risk of developing heart disease.”

Purpose of Hypothesis

The purpose of a hypothesis is to provide a testable explanation for an observed phenomenon or a prediction of a future outcome based on existing knowledge or theories. A hypothesis is an essential part of the scientific method and helps to guide the research process by providing a clear focus for investigation. It enables scientists to design experiments or studies to gather evidence and data that can support or refute the proposed explanation or prediction.

The formulation of a hypothesis is based on existing knowledge, observations, and theories, and it should be specific, testable, and falsifiable. A specific hypothesis helps to define the research question, which is important in the research process as it guides the selection of an appropriate research design and methodology. Testability of the hypothesis means that it can be proven or disproven through empirical data collection and analysis. Falsifiability means that the hypothesis should be formulated in such a way that it can be proven wrong if it is incorrect.

In addition to guiding the research process, the testing of hypotheses can lead to new discoveries and advancements in scientific knowledge. When a hypothesis is supported by the data, it can be used to develop new theories or models to explain the observed phenomenon. When a hypothesis is not supported by the data, it can help to refine existing theories or prompt the development of new hypotheses to explain the phenomenon.

When to use Hypothesis

Here are some common situations in which hypotheses are used:

  • In scientific research , hypotheses are used to guide the design of experiments and to help researchers make predictions about the outcomes of those experiments.
  • In social science research , hypotheses are used to test theories about human behavior, social relationships, and other phenomena.
  • I n business , hypotheses can be used to guide decisions about marketing, product development, and other areas. For example, a hypothesis might be that a new product will sell well in a particular market, and this hypothesis can be tested through market research.

Characteristics of Hypothesis

Here are some common characteristics of a hypothesis:

  • Testable : A hypothesis must be able to be tested through observation or experimentation. This means that it must be possible to collect data that will either support or refute the hypothesis.
  • Falsifiable : A hypothesis must be able to be proven false if it is not supported by the data. If a hypothesis cannot be falsified, then it is not a scientific hypothesis.
  • Clear and concise : A hypothesis should be stated in a clear and concise manner so that it can be easily understood and tested.
  • Based on existing knowledge : A hypothesis should be based on existing knowledge and research in the field. It should not be based on personal beliefs or opinions.
  • Specific : A hypothesis should be specific in terms of the variables being tested and the predicted outcome. This will help to ensure that the research is focused and well-designed.
  • Tentative: A hypothesis is a tentative statement or assumption that requires further testing and evidence to be confirmed or refuted. It is not a final conclusion or assertion.
  • Relevant : A hypothesis should be relevant to the research question or problem being studied. It should address a gap in knowledge or provide a new perspective on the issue.

Advantages of Hypothesis

Hypotheses have several advantages in scientific research and experimentation:

  • Guides research: A hypothesis provides a clear and specific direction for research. It helps to focus the research question, select appropriate methods and variables, and interpret the results.
  • Predictive powe r: A hypothesis makes predictions about the outcome of research, which can be tested through experimentation. This allows researchers to evaluate the validity of the hypothesis and make new discoveries.
  • Facilitates communication: A hypothesis provides a common language and framework for scientists to communicate with one another about their research. This helps to facilitate the exchange of ideas and promotes collaboration.
  • Efficient use of resources: A hypothesis helps researchers to use their time, resources, and funding efficiently by directing them towards specific research questions and methods that are most likely to yield results.
  • Provides a basis for further research: A hypothesis that is supported by data provides a basis for further research and exploration. It can lead to new hypotheses, theories, and discoveries.
  • Increases objectivity: A hypothesis can help to increase objectivity in research by providing a clear and specific framework for testing and interpreting results. This can reduce bias and increase the reliability of research findings.

Limitations of Hypothesis

Some Limitations of the Hypothesis are as follows:

  • Limited to observable phenomena: Hypotheses are limited to observable phenomena and cannot account for unobservable or intangible factors. This means that some research questions may not be amenable to hypothesis testing.
  • May be inaccurate or incomplete: Hypotheses are based on existing knowledge and research, which may be incomplete or inaccurate. This can lead to flawed hypotheses and erroneous conclusions.
  • May be biased: Hypotheses may be biased by the researcher’s own beliefs, values, or assumptions. This can lead to selective interpretation of data and a lack of objectivity in research.
  • Cannot prove causation: A hypothesis can only show a correlation between variables, but it cannot prove causation. This requires further experimentation and analysis.
  • Limited to specific contexts: Hypotheses are limited to specific contexts and may not be generalizable to other situations or populations. This means that results may not be applicable in other contexts or may require further testing.
  • May be affected by chance : Hypotheses may be affected by chance or random variation, which can obscure or distort the true relationship between variables.

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What is a scientific hypothesis?

It's the initial building block in the scientific method.

A girl looks at plants in a test tube for a science experiment. What's her scientific hypothesis?

Hypothesis basics

What makes a hypothesis testable.

  • Types of hypotheses
  • Hypothesis versus theory

Additional resources

Bibliography.

A scientific hypothesis is a tentative, testable explanation for a phenomenon in the natural world. It's the initial building block in the scientific method . Many describe it as an "educated guess" based on prior knowledge and observation. While this is true, a hypothesis is more informed than a guess. While an "educated guess" suggests a random prediction based on a person's expertise, developing a hypothesis requires active observation and background research. 

The basic idea of a hypothesis is that there is no predetermined outcome. For a solution to be termed a scientific hypothesis, it has to be an idea that can be supported or refuted through carefully crafted experimentation or observation. This concept, called falsifiability and testability, was advanced in the mid-20th century by Austrian-British philosopher Karl Popper in his famous book "The Logic of Scientific Discovery" (Routledge, 1959).

A key function of a hypothesis is to derive predictions about the results of future experiments and then perform those experiments to see whether they support the predictions.

A hypothesis is usually written in the form of an if-then statement, which gives a possibility (if) and explains what may happen because of the possibility (then). The statement could also include "may," according to California State University, Bakersfield .

Here are some examples of hypothesis statements:

  • If garlic repels fleas, then a dog that is given garlic every day will not get fleas.
  • If sugar causes cavities, then people who eat a lot of candy may be more prone to cavities.
  • If ultraviolet light can damage the eyes, then maybe this light can cause blindness.

A useful hypothesis should be testable and falsifiable. That means that it should be possible to prove it wrong. A theory that can't be proved wrong is nonscientific, according to Karl Popper's 1963 book " Conjectures and Refutations ."

An example of an untestable statement is, "Dogs are better than cats." That's because the definition of "better" is vague and subjective. However, an untestable statement can be reworded to make it testable. For example, the previous statement could be changed to this: "Owning a dog is associated with higher levels of physical fitness than owning a cat." With this statement, the researcher can take measures of physical fitness from dog and cat owners and compare the two.

Types of scientific hypotheses

Elementary-age students study alternative energy using homemade windmills during public school science class.

In an experiment, researchers generally state their hypotheses in two ways. The null hypothesis predicts that there will be no relationship between the variables tested, or no difference between the experimental groups. The alternative hypothesis predicts the opposite: that there will be a difference between the experimental groups. This is usually the hypothesis scientists are most interested in, according to the University of Miami .

For example, a null hypothesis might state, "There will be no difference in the rate of muscle growth between people who take a protein supplement and people who don't." The alternative hypothesis would state, "There will be a difference in the rate of muscle growth between people who take a protein supplement and people who don't."

If the results of the experiment show a relationship between the variables, then the null hypothesis has been rejected in favor of the alternative hypothesis, according to the book " Research Methods in Psychology " (​​BCcampus, 2015). 

There are other ways to describe an alternative hypothesis. The alternative hypothesis above does not specify a direction of the effect, only that there will be a difference between the two groups. That type of prediction is called a two-tailed hypothesis. If a hypothesis specifies a certain direction — for example, that people who take a protein supplement will gain more muscle than people who don't — it is called a one-tailed hypothesis, according to William M. K. Trochim , a professor of Policy Analysis and Management at Cornell University.

Sometimes, errors take place during an experiment. These errors can happen in one of two ways. A type I error is when the null hypothesis is rejected when it is true. This is also known as a false positive. A type II error occurs when the null hypothesis is not rejected when it is false. This is also known as a false negative, according to the University of California, Berkeley . 

A hypothesis can be rejected or modified, but it can never be proved correct 100% of the time. For example, a scientist can form a hypothesis stating that if a certain type of tomato has a gene for red pigment, that type of tomato will be red. During research, the scientist then finds that each tomato of this type is red. Though the findings confirm the hypothesis, there may be a tomato of that type somewhere in the world that isn't red. Thus, the hypothesis is true, but it may not be true 100% of the time.

Scientific theory vs. scientific hypothesis

The best hypotheses are simple. They deal with a relatively narrow set of phenomena. But theories are broader; they generally combine multiple hypotheses into a general explanation for a wide range of phenomena, according to the University of California, Berkeley . For example, a hypothesis might state, "If animals adapt to suit their environments, then birds that live on islands with lots of seeds to eat will have differently shaped beaks than birds that live on islands with lots of insects to eat." After testing many hypotheses like these, Charles Darwin formulated an overarching theory: the theory of evolution by natural selection.

"Theories are the ways that we make sense of what we observe in the natural world," Tanner said. "Theories are structures of ideas that explain and interpret facts." 

  • Read more about writing a hypothesis, from the American Medical Writers Association.
  • Find out why a hypothesis isn't always necessary in science, from The American Biology Teacher.
  • Learn about null and alternative hypotheses, from Prof. Essa on YouTube .

Encyclopedia Britannica. Scientific Hypothesis. Jan. 13, 2022. https://www.britannica.com/science/scientific-hypothesis

Karl Popper, "The Logic of Scientific Discovery," Routledge, 1959.

California State University, Bakersfield, "Formatting a testable hypothesis." https://www.csub.edu/~ddodenhoff/Bio100/Bio100sp04/formattingahypothesis.htm  

Karl Popper, "Conjectures and Refutations," Routledge, 1963.

Price, P., Jhangiani, R., & Chiang, I., "Research Methods of Psychology — 2nd Canadian Edition," BCcampus, 2015.‌

University of Miami, "The Scientific Method" http://www.bio.miami.edu/dana/161/evolution/161app1_scimethod.pdf  

William M.K. Trochim, "Research Methods Knowledge Base," https://conjointly.com/kb/hypotheses-explained/  

University of California, Berkeley, "Multiple Hypothesis Testing and False Discovery Rate" https://www.stat.berkeley.edu/~hhuang/STAT141/Lecture-FDR.pdf  

University of California, Berkeley, "Science at multiple levels" https://undsci.berkeley.edu/article/0_0_0/howscienceworks_19

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Definition of hypothesis

Did you know.

The Difference Between Hypothesis and Theory

A hypothesis is an assumption, an idea that is proposed for the sake of argument so that it can be tested to see if it might be true.

In the scientific method, the hypothesis is constructed before any applicable research has been done, apart from a basic background review. You ask a question, read up on what has been studied before, and then form a hypothesis.

A hypothesis is usually tentative; it's an assumption or suggestion made strictly for the objective of being tested.

A theory , in contrast, is a principle that has been formed as an attempt to explain things that have already been substantiated by data. It is used in the names of a number of principles accepted in the scientific community, such as the Big Bang Theory . Because of the rigors of experimentation and control, it is understood to be more likely to be true than a hypothesis is.

In non-scientific use, however, hypothesis and theory are often used interchangeably to mean simply an idea, speculation, or hunch, with theory being the more common choice.

Since this casual use does away with the distinctions upheld by the scientific community, hypothesis and theory are prone to being wrongly interpreted even when they are encountered in scientific contexts—or at least, contexts that allude to scientific study without making the critical distinction that scientists employ when weighing hypotheses and theories.

The most common occurrence is when theory is interpreted—and sometimes even gleefully seized upon—to mean something having less truth value than other scientific principles. (The word law applies to principles so firmly established that they are almost never questioned, such as the law of gravity.)

This mistake is one of projection: since we use theory in general to mean something lightly speculated, then it's implied that scientists must be talking about the same level of uncertainty when they use theory to refer to their well-tested and reasoned principles.

The distinction has come to the forefront particularly on occasions when the content of science curricula in schools has been challenged—notably, when a school board in Georgia put stickers on textbooks stating that evolution was "a theory, not a fact, regarding the origin of living things." As Kenneth R. Miller, a cell biologist at Brown University, has said , a theory "doesn’t mean a hunch or a guess. A theory is a system of explanations that ties together a whole bunch of facts. It not only explains those facts, but predicts what you ought to find from other observations and experiments.”

While theories are never completely infallible, they form the basis of scientific reasoning because, as Miller said "to the best of our ability, we’ve tested them, and they’ve held up."

  • proposition
  • supposition

hypothesis , theory , law mean a formula derived by inference from scientific data that explains a principle operating in nature.

hypothesis implies insufficient evidence to provide more than a tentative explanation.

theory implies a greater range of evidence and greater likelihood of truth.

law implies a statement of order and relation in nature that has been found to be invariable under the same conditions.

Examples of hypothesis in a Sentence

These examples are programmatically compiled from various online sources to illustrate current usage of the word 'hypothesis.' Any opinions expressed in the examples do not represent those of Merriam-Webster or its editors. Send us feedback about these examples.

Word History

Greek, from hypotithenai to put under, suppose, from hypo- + tithenai to put — more at do

1641, in the meaning defined at sense 1a

Phrases Containing hypothesis

  • counter - hypothesis
  • nebular hypothesis
  • null hypothesis
  • planetesimal hypothesis
  • Whorfian hypothesis

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“Hypothesis.” Merriam-Webster.com Dictionary , Merriam-Webster, https://www.merriam-webster.com/dictionary/hypothesis. Accessed 12 May. 2024.

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How to Write a Great Hypothesis

Hypothesis Definition, Format, Examples, and Tips

Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

key hypothesis meaning

Amy Morin, LCSW, is a psychotherapist and international bestselling author. Her books, including "13 Things Mentally Strong People Don't Do," have been translated into more than 40 languages. Her TEDx talk,  "The Secret of Becoming Mentally Strong," is one of the most viewed talks of all time.

key hypothesis meaning

Verywell / Alex Dos Diaz

  • The Scientific Method

Hypothesis Format

Falsifiability of a hypothesis.

  • Operationalization

Hypothesis Types

Hypotheses examples.

  • Collecting Data

A hypothesis is a tentative statement about the relationship between two or more variables. It is a specific, testable prediction about what you expect to happen in a study. It is a preliminary answer to your question that helps guide the research process.

Consider a study designed to examine the relationship between sleep deprivation and test performance. The hypothesis might be: "This study is designed to assess the hypothesis that sleep-deprived people will perform worse on a test than individuals who are not sleep-deprived."

At a Glance

A hypothesis is crucial to scientific research because it offers a clear direction for what the researchers are looking to find. This allows them to design experiments to test their predictions and add to our scientific knowledge about the world. This article explores how a hypothesis is used in psychology research, how to write a good hypothesis, and the different types of hypotheses you might use.

The Hypothesis in the Scientific Method

In the scientific method , whether it involves research in psychology, biology, or some other area, a hypothesis represents what the researchers think will happen in an experiment. The scientific method involves the following steps:

  • Forming a question
  • Performing background research
  • Creating a hypothesis
  • Designing an experiment
  • Collecting data
  • Analyzing the results
  • Drawing conclusions
  • Communicating the results

The hypothesis is a prediction, but it involves more than a guess. Most of the time, the hypothesis begins with a question which is then explored through background research. At this point, researchers then begin to develop a testable hypothesis.

Unless you are creating an exploratory study, your hypothesis should always explain what you  expect  to happen.

In a study exploring the effects of a particular drug, the hypothesis might be that researchers expect the drug to have some type of effect on the symptoms of a specific illness. In psychology, the hypothesis might focus on how a certain aspect of the environment might influence a particular behavior.

Remember, a hypothesis does not have to be correct. While the hypothesis predicts what the researchers expect to see, the goal of the research is to determine whether this guess is right or wrong. When conducting an experiment, researchers might explore numerous factors to determine which ones might contribute to the ultimate outcome.

In many cases, researchers may find that the results of an experiment  do not  support the original hypothesis. When writing up these results, the researchers might suggest other options that should be explored in future studies.

In many cases, researchers might draw a hypothesis from a specific theory or build on previous research. For example, prior research has shown that stress can impact the immune system. So a researcher might hypothesize: "People with high-stress levels will be more likely to contract a common cold after being exposed to the virus than people who have low-stress levels."

In other instances, researchers might look at commonly held beliefs or folk wisdom. "Birds of a feather flock together" is one example of folk adage that a psychologist might try to investigate. The researcher might pose a specific hypothesis that "People tend to select romantic partners who are similar to them in interests and educational level."

Elements of a Good Hypothesis

So how do you write a good hypothesis? When trying to come up with a hypothesis for your research or experiments, ask yourself the following questions:

  • Is your hypothesis based on your research on a topic?
  • Can your hypothesis be tested?
  • Does your hypothesis include independent and dependent variables?

Before you come up with a specific hypothesis, spend some time doing background research. Once you have completed a literature review, start thinking about potential questions you still have. Pay attention to the discussion section in the  journal articles you read . Many authors will suggest questions that still need to be explored.

How to Formulate a Good Hypothesis

To form a hypothesis, you should take these steps:

  • Collect as many observations about a topic or problem as you can.
  • Evaluate these observations and look for possible causes of the problem.
  • Create a list of possible explanations that you might want to explore.
  • After you have developed some possible hypotheses, think of ways that you could confirm or disprove each hypothesis through experimentation. This is known as falsifiability.

In the scientific method ,  falsifiability is an important part of any valid hypothesis. In order to test a claim scientifically, it must be possible that the claim could be proven false.

Students sometimes confuse the idea of falsifiability with the idea that it means that something is false, which is not the case. What falsifiability means is that  if  something was false, then it is possible to demonstrate that it is false.

One of the hallmarks of pseudoscience is that it makes claims that cannot be refuted or proven false.

The Importance of Operational Definitions

A variable is a factor or element that can be changed and manipulated in ways that are observable and measurable. However, the researcher must also define how the variable will be manipulated and measured in the study.

Operational definitions are specific definitions for all relevant factors in a study. This process helps make vague or ambiguous concepts detailed and measurable.

For example, a researcher might operationally define the variable " test anxiety " as the results of a self-report measure of anxiety experienced during an exam. A "study habits" variable might be defined by the amount of studying that actually occurs as measured by time.

These precise descriptions are important because many things can be measured in various ways. Clearly defining these variables and how they are measured helps ensure that other researchers can replicate your results.

Replicability

One of the basic principles of any type of scientific research is that the results must be replicable.

Replication means repeating an experiment in the same way to produce the same results. By clearly detailing the specifics of how the variables were measured and manipulated, other researchers can better understand the results and repeat the study if needed.

Some variables are more difficult than others to define. For example, how would you operationally define a variable such as aggression ? For obvious ethical reasons, researchers cannot create a situation in which a person behaves aggressively toward others.

To measure this variable, the researcher must devise a measurement that assesses aggressive behavior without harming others. The researcher might utilize a simulated task to measure aggressiveness in this situation.

Hypothesis Checklist

  • Does your hypothesis focus on something that you can actually test?
  • Does your hypothesis include both an independent and dependent variable?
  • Can you manipulate the variables?
  • Can your hypothesis be tested without violating ethical standards?

The hypothesis you use will depend on what you are investigating and hoping to find. Some of the main types of hypotheses that you might use include:

  • Simple hypothesis : This type of hypothesis suggests there is a relationship between one independent variable and one dependent variable.
  • Complex hypothesis : This type suggests a relationship between three or more variables, such as two independent and dependent variables.
  • Null hypothesis : This hypothesis suggests no relationship exists between two or more variables.
  • Alternative hypothesis : This hypothesis states the opposite of the null hypothesis.
  • Statistical hypothesis : This hypothesis uses statistical analysis to evaluate a representative population sample and then generalizes the findings to the larger group.
  • Logical hypothesis : This hypothesis assumes a relationship between variables without collecting data or evidence.

A hypothesis often follows a basic format of "If {this happens} then {this will happen}." One way to structure your hypothesis is to describe what will happen to the  dependent variable  if you change the  independent variable .

The basic format might be: "If {these changes are made to a certain independent variable}, then we will observe {a change in a specific dependent variable}."

A few examples of simple hypotheses:

  • "Students who eat breakfast will perform better on a math exam than students who do not eat breakfast."
  • "Students who experience test anxiety before an English exam will get lower scores than students who do not experience test anxiety."​
  • "Motorists who talk on the phone while driving will be more likely to make errors on a driving course than those who do not talk on the phone."
  • "Children who receive a new reading intervention will have higher reading scores than students who do not receive the intervention."

Examples of a complex hypothesis include:

  • "People with high-sugar diets and sedentary activity levels are more likely to develop depression."
  • "Younger people who are regularly exposed to green, outdoor areas have better subjective well-being than older adults who have limited exposure to green spaces."

Examples of a null hypothesis include:

  • "There is no difference in anxiety levels between people who take St. John's wort supplements and those who do not."
  • "There is no difference in scores on a memory recall task between children and adults."
  • "There is no difference in aggression levels between children who play first-person shooter games and those who do not."

Examples of an alternative hypothesis:

  • "People who take St. John's wort supplements will have less anxiety than those who do not."
  • "Adults will perform better on a memory task than children."
  • "Children who play first-person shooter games will show higher levels of aggression than children who do not." 

Collecting Data on Your Hypothesis

Once a researcher has formed a testable hypothesis, the next step is to select a research design and start collecting data. The research method depends largely on exactly what they are studying. There are two basic types of research methods: descriptive research and experimental research.

Descriptive Research Methods

Descriptive research such as  case studies ,  naturalistic observations , and surveys are often used when  conducting an experiment is difficult or impossible. These methods are best used to describe different aspects of a behavior or psychological phenomenon.

Once a researcher has collected data using descriptive methods, a  correlational study  can examine how the variables are related. This research method might be used to investigate a hypothesis that is difficult to test experimentally.

Experimental Research Methods

Experimental methods  are used to demonstrate causal relationships between variables. In an experiment, the researcher systematically manipulates a variable of interest (known as the independent variable) and measures the effect on another variable (known as the dependent variable).

Unlike correlational studies, which can only be used to determine if there is a relationship between two variables, experimental methods can be used to determine the actual nature of the relationship—whether changes in one variable actually  cause  another to change.

The hypothesis is a critical part of any scientific exploration. It represents what researchers expect to find in a study or experiment. In situations where the hypothesis is unsupported by the research, the research still has value. Such research helps us better understand how different aspects of the natural world relate to one another. It also helps us develop new hypotheses that can then be tested in the future.

Thompson WH, Skau S. On the scope of scientific hypotheses .  R Soc Open Sci . 2023;10(8):230607. doi:10.1098/rsos.230607

Taran S, Adhikari NKJ, Fan E. Falsifiability in medicine: what clinicians can learn from Karl Popper [published correction appears in Intensive Care Med. 2021 Jun 17;:].  Intensive Care Med . 2021;47(9):1054-1056. doi:10.1007/s00134-021-06432-z

Eyler AA. Research Methods for Public Health . 1st ed. Springer Publishing Company; 2020. doi:10.1891/9780826182067.0004

Nosek BA, Errington TM. What is replication ?  PLoS Biol . 2020;18(3):e3000691. doi:10.1371/journal.pbio.3000691

Aggarwal R, Ranganathan P. Study designs: Part 2 - Descriptive studies .  Perspect Clin Res . 2019;10(1):34-36. doi:10.4103/picr.PICR_154_18

Nevid J. Psychology: Concepts and Applications. Wadworth, 2013.

By Kendra Cherry, MSEd Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

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Basic Theory

Preliminaries.

As usual, our starting point is a random experiment with an underlying sample space and a probability measure \(\P\). In the basic statistical model, we have an observable random variable \(\bs{X}\) taking values in a set \(S\). In general, \(\bs{X}\) can have quite a complicated structure. For example, if the experiment is to sample \(n\) objects from a population and record various measurements of interest, then \[ \bs{X} = (X_1, X_2, \ldots, X_n) \] where \(X_i\) is the vector of measurements for the \(i\)th object. The most important special case occurs when \((X_1, X_2, \ldots, X_n)\) are independent and identically distributed. In this case, we have a random sample of size \(n\) from the common distribution.

The purpose of this section is to define and discuss the basic concepts of statistical hypothesis testing . Collectively, these concepts are sometimes referred to as the Neyman-Pearson framework, in honor of Jerzy Neyman and Egon Pearson, who first formalized them.

A statistical hypothesis is a statement about the distribution of \(\bs{X}\). Equivalently, a statistical hypothesis specifies a set of possible distributions of \(\bs{X}\): the set of distributions for which the statement is true. A hypothesis that specifies a single distribution for \(\bs{X}\) is called simple ; a hypothesis that specifies more than one distribution for \(\bs{X}\) is called composite .

In hypothesis testing , the goal is to see if there is sufficient statistical evidence to reject a presumed null hypothesis in favor of a conjectured alternative hypothesis . The null hypothesis is usually denoted \(H_0\) while the alternative hypothesis is usually denoted \(H_1\).

An hypothesis test is a statistical decision ; the conclusion will either be to reject the null hypothesis in favor of the alternative, or to fail to reject the null hypothesis. The decision that we make must, of course, be based on the observed value \(\bs{x}\) of the data vector \(\bs{X}\). Thus, we will find an appropriate subset \(R\) of the sample space \(S\) and reject \(H_0\) if and only if \(\bs{x} \in R\). The set \(R\) is known as the rejection region or the critical region . Note the asymmetry between the null and alternative hypotheses. This asymmetry is due to the fact that we assume the null hypothesis, in a sense, and then see if there is sufficient evidence in \(\bs{x}\) to overturn this assumption in favor of the alternative.

An hypothesis test is a statistical analogy to proof by contradiction, in a sense. Suppose for a moment that \(H_1\) is a statement in a mathematical theory and that \(H_0\) is its negation. One way that we can prove \(H_1\) is to assume \(H_0\) and work our way logically to a contradiction. In an hypothesis test, we don't prove anything of course, but there are similarities. We assume \(H_0\) and then see if the data \(\bs{x}\) are sufficiently at odds with that assumption that we feel justified in rejecting \(H_0\) in favor of \(H_1\).

Often, the critical region is defined in terms of a statistic \(w(\bs{X})\), known as a test statistic , where \(w\) is a function from \(S\) into another set \(T\). We find an appropriate rejection region \(R_T \subseteq T\) and reject \(H_0\) when the observed value \(w(\bs{x}) \in R_T\). Thus, the rejection region in \(S\) is then \(R = w^{-1}(R_T) = \left\{\bs{x} \in S: w(\bs{x}) \in R_T\right\}\). As usual, the use of a statistic often allows significant data reduction when the dimension of the test statistic is much smaller than the dimension of the data vector.

The ultimate decision may be correct or may be in error. There are two types of errors, depending on which of the hypotheses is actually true.

Types of errors:

  • A type 1 error is rejecting the null hypothesis \(H_0\) when \(H_0\) is true.
  • A type 2 error is failing to reject the null hypothesis \(H_0\) when the alternative hypothesis \(H_1\) is true.

Similarly, there are two ways to make a correct decision: we could reject \(H_0\) when \(H_1\) is true or we could fail to reject \(H_0\) when \(H_0\) is true. The possibilities are summarized in the following table:

Of course, when we observe \(\bs{X} = \bs{x}\) and make our decision, either we will have made the correct decision or we will have committed an error, and usually we will never know which of these events has occurred. Prior to gathering the data, however, we can consider the probabilities of the various errors.

If \(H_0\) is true (that is, the distribution of \(\bs{X}\) is specified by \(H_0\)), then \(\P(\bs{X} \in R)\) is the probability of a type 1 error for this distribution. If \(H_0\) is composite, then \(H_0\) specifies a variety of different distributions for \(\bs{X}\) and thus there is a set of type 1 error probabilities.

The maximum probability of a type 1 error, over the set of distributions specified by \( H_0 \), is the significance level of the test or the size of the critical region.

The significance level is often denoted by \(\alpha\). Usually, the rejection region is constructed so that the significance level is a prescribed, small value (typically 0.1, 0.05, 0.01).

If \(H_1\) is true (that is, the distribution of \(\bs{X}\) is specified by \(H_1\)), then \(\P(\bs{X} \notin R)\) is the probability of a type 2 error for this distribution. Again, if \(H_1\) is composite then \(H_1\) specifies a variety of different distributions for \(\bs{X}\), and thus there will be a set of type 2 error probabilities. Generally, there is a tradeoff between the type 1 and type 2 error probabilities. If we reduce the probability of a type 1 error, by making the rejection region \(R\) smaller, we necessarily increase the probability of a type 2 error because the complementary region \(S \setminus R\) is larger.

The extreme cases can give us some insight. First consider the decision rule in which we never reject \(H_0\), regardless of the evidence \(\bs{x}\). This corresponds to the rejection region \(R = \emptyset\). A type 1 error is impossible, so the significance level is 0. On the other hand, the probability of a type 2 error is 1 for any distribution defined by \(H_1\). At the other extreme, consider the decision rule in which we always rejects \(H_0\) regardless of the evidence \(\bs{x}\). This corresponds to the rejection region \(R = S\). A type 2 error is impossible, but now the probability of a type 1 error is 1 for any distribution defined by \(H_0\). In between these two worthless tests are meaningful tests that take the evidence \(\bs{x}\) into account.

If \(H_1\) is true, so that the distribution of \(\bs{X}\) is specified by \(H_1\), then \(\P(\bs{X} \in R)\), the probability of rejecting \(H_0\) is the power of the test for that distribution.

Thus the power of the test for a distribution specified by \( H_1 \) is the probability of making the correct decision.

Suppose that we have two tests, corresponding to rejection regions \(R_1\) and \(R_2\), respectively, each having significance level \(\alpha\). The test with region \(R_1\) is uniformly more powerful than the test with region \(R_2\) if \[ \P(\bs{X} \in R_1) \ge \P(\bs{X} \in R_2) \text{ for every distribution of } \bs{X} \text{ specified by } H_1 \]

Naturally, in this case, we would prefer the first test. Often, however, two tests will not be uniformly ordered; one test will be more powerful for some distributions specified by \(H_1\) while the other test will be more powerful for other distributions specified by \(H_1\).

If a test has significance level \(\alpha\) and is uniformly more powerful than any other test with significance level \(\alpha\), then the test is said to be a uniformly most powerful test at level \(\alpha\).

Clearly a uniformly most powerful test is the best we can do.

\(P\)-value

In most cases, we have a general procedure that allows us to construct a test (that is, a rejection region \(R_\alpha\)) for any given significance level \(\alpha \in (0, 1)\). Typically, \(R_\alpha\) decreases (in the subset sense) as \(\alpha\) decreases.

The \(P\)-value of the observed value \(\bs{x}\) of \(\bs{X}\), denoted \(P(\bs{x})\), is defined to be the smallest \(\alpha\) for which \(\bs{x} \in R_\alpha\); that is, the smallest significance level for which \(H_0\) is rejected, given \(\bs{X} = \bs{x}\).

Knowing \(P(\bs{x})\) allows us to test \(H_0\) at any significance level for the given data \(\bs{x}\): If \(P(\bs{x}) \le \alpha\) then we would reject \(H_0\) at significance level \(\alpha\); if \(P(\bs{x}) \gt \alpha\) then we fail to reject \(H_0\) at significance level \(\alpha\). Note that \(P(\bs{X})\) is a statistic . Informally, \(P(\bs{x})\) can often be thought of as the probability of an outcome as or more extreme than the observed value \(\bs{x}\), where extreme is interpreted relative to the null hypothesis \(H_0\).

Analogy with Justice Systems

There is a helpful analogy between statistical hypothesis testing and the criminal justice system in the US and various other countries. Consider a person charged with a crime. The presumed null hypothesis is that the person is innocent of the crime; the conjectured alternative hypothesis is that the person is guilty of the crime. The test of the hypotheses is a trial with evidence presented by both sides playing the role of the data. After considering the evidence, the jury delivers the decision as either not guilty or guilty . Note that innocent is not a possible verdict of the jury, because it is not the point of the trial to prove the person innocent. Rather, the point of the trial is to see whether there is sufficient evidence to overturn the null hypothesis that the person is innocent in favor of the alternative hypothesis of that the person is guilty. A type 1 error is convicting a person who is innocent; a type 2 error is acquitting a person who is guilty. Generally, a type 1 error is considered the more serious of the two possible errors, so in an attempt to hold the chance of a type 1 error to a very low level, the standard for conviction in serious criminal cases is beyond a reasonable doubt .

Tests of an Unknown Parameter

Hypothesis testing is a very general concept, but an important special class occurs when the distribution of the data variable \(\bs{X}\) depends on a parameter \(\theta\) taking values in a parameter space \(\Theta\). The parameter may be vector-valued, so that \(\bs{\theta} = (\theta_1, \theta_2, \ldots, \theta_n)\) and \(\Theta \subseteq \R^k\) for some \(k \in \N_+\). The hypotheses generally take the form \[ H_0: \theta \in \Theta_0 \text{ versus } H_1: \theta \notin \Theta_0 \] where \(\Theta_0\) is a prescribed subset of the parameter space \(\Theta\). In this setting, the probabilities of making an error or a correct decision depend on the true value of \(\theta\). If \(R\) is the rejection region, then the power function \( Q \) is given by \[ Q(\theta) = \P_\theta(\bs{X} \in R), \quad \theta \in \Theta \] The power function gives a lot of information about the test.

The power function satisfies the following properties:

  • \(Q(\theta)\) is the probability of a type 1 error when \(\theta \in \Theta_0\).
  • \(\max\left\{Q(\theta): \theta \in \Theta_0\right\}\) is the significance level of the test.
  • \(1 - Q(\theta)\) is the probability of a type 2 error when \(\theta \notin \Theta_0\).
  • \(Q(\theta)\) is the power of the test when \(\theta \notin \Theta_0\).

If we have two tests, we can compare them by means of their power functions.

Suppose that we have two tests, corresponding to rejection regions \(R_1\) and \(R_2\), respectively, each having significance level \(\alpha\). The test with rejection region \(R_1\) is uniformly more powerful than the test with rejection region \(R_2\) if \( Q_1(\theta) \ge Q_2(\theta)\) for all \( \theta \notin \Theta_0 \).

Most hypothesis tests of an unknown real parameter \(\theta\) fall into three special cases:

Suppose that \( \theta \) is a real parameter and \( \theta_0 \in \Theta \) a specified value. The tests below are respectively the two-sided test , the left-tailed test , and the right-tailed test .

  • \(H_0: \theta = \theta_0\) versus \(H_1: \theta \ne \theta_0\)
  • \(H_0: \theta \ge \theta_0\) versus \(H_1: \theta \lt \theta_0\)
  • \(H_0: \theta \le \theta_0\) versus \(H_1: \theta \gt \theta_0\)

Thus the tests are named after the conjectured alternative. Of course, there may be other unknown parameters besides \(\theta\) (known as nuisance parameters ).

Equivalence Between Hypothesis Test and Confidence Sets

There is an equivalence between hypothesis tests and confidence sets for a parameter \(\theta\).

Suppose that \(C(\bs{x})\) is a \(1 - \alpha\) level confidence set for \(\theta\). The following test has significance level \(\alpha\) for the hypothesis \( H_0: \theta = \theta_0 \) versus \( H_1: \theta \ne \theta_0 \): Reject \(H_0\) if and only if \(\theta_0 \notin C(\bs{x})\)

By definition, \(\P[\theta \in C(\bs{X})] = 1 - \alpha\). Hence if \(H_0\) is true so that \(\theta = \theta_0\), then the probability of a type 1 error is \(P[\theta \notin C(\bs{X})] = \alpha\).

Equivalently, we fail to reject \(H_0\) at significance level \(\alpha\) if and only if \(\theta_0\) is in the corresponding \(1 - \alpha\) level confidence set. In particular, this equivalence applies to interval estimates of a real parameter \(\theta\) and the common tests for \(\theta\) given above .

In each case below, the confidence interval has confidence level \(1 - \alpha\) and the test has significance level \(\alpha\).

  • Suppose that \(\left[L(\bs{X}, U(\bs{X})\right]\) is a two-sided confidence interval for \(\theta\). Reject \(H_0: \theta = \theta_0\) versus \(H_1: \theta \ne \theta_0\) if and only if \(\theta_0 \lt L(\bs{X})\) or \(\theta_0 \gt U(\bs{X})\).
  • Suppose that \(L(\bs{X})\) is a confidence lower bound for \(\theta\). Reject \(H_0: \theta \le \theta_0\) versus \(H_1: \theta \gt \theta_0\) if and only if \(\theta_0 \lt L(\bs{X})\).
  • Suppose that \(U(\bs{X})\) is a confidence upper bound for \(\theta\). Reject \(H_0: \theta \ge \theta_0\) versus \(H_1: \theta \lt \theta_0\) if and only if \(\theta_0 \gt U(\bs{X})\).

Pivot Variables and Test Statistics

Recall that confidence sets of an unknown parameter \(\theta\) are often constructed through a pivot variable , that is, a random variable \(W(\bs{X}, \theta)\) that depends on the data vector \(\bs{X}\) and the parameter \(\theta\), but whose distribution does not depend on \(\theta\) and is known. In this case, a natural test statistic for the basic tests given above is \(W(\bs{X}, \theta_0)\).

Define Hypothesis: Unveiling the First Step in Scientific Inquiry

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Welcome to the world of research, where you’ll journey through a universe brimming with questions and curiosity. In this cosmos, a hypothesis is one celestial object you can’t miss! Today’s expedition invites you on board an exploration to ‘Define Hypothesis.’ Hop in; it wouldn’t be hyperbole to state we’re about to unlock the nucleus behind every ever scientific theory and inquiry!

Definition of Hypothesis

Introduction to the concept of hypothesis.

Picture yourself as a detective solving a case. Right from inspecting clues, formulating potential theories on whodunit, putting these theories under rigorous tests until finally reaching that elusive conclusive evidence – exciting, isn’t it?

Now replace detective with researcher and voila – here comes our heavyweight term: Hypothesis. Much like how any plausible theory drives detectives’ investigations, scientific hypotheses are vital navigational compasses guiding researchers in their quest for scientific evolutions.

Explanation of What a Hypothesis Is in The Context of Research and Scientific Inquiry

A hypothesis – popularly known as an educated guess or predictive statement – represents an initial supposition or proposed explanation made on limited information but founded on validation-grounded knowledge. It forms the basis for preliminary exploration into a specific set of circumstances or natural phenomena beyond.

Formulated prior to conducting research, scientists employ hypotheses as testable conjectures to explain an observed behavior or event. Confused? Fret not. To put it simply and by example: “If I increase the frequency of watering my plants twice daily (instead of solely relying upon weather conditions), then they will grow faster.” Now that’s what we call an everyday-life hypothesis!

Remember, hypotheses are not wild guesses plucked out of thin air but rather preconceived assertions open to empirical verification. They mark the inception point for any scientific investigation and serve as cornerstones for further experiments.

Characteristics and Components of a Hypothesis

Key characteristics of a hypothesis.

Before plunging into the deep end to define a hypothesis, let’s brush up on the features that contribute to effective hypotheses. For starters, a strong hypothesis is testable. This means it must be possible for empirical evidence to either support the word hypothesis or contradict it. The proposal should also be logically consistent and grounded firmly in existing knowledge.

Further down the line, another salient feature is specificity. Good hypotheses are not broad statements but instead focus on a specific aspect or phenomenon within the intended research field. Moreover, they are typically succinct and easily understandable ensuring information isn’t lost in translation among researchers.

Moreover, any well-structured hypothesis connects the independent and dependent variables together – typically, there’s at least one independent and one dependent variable involved. These elements form a relationship where changes instigated in the independent variable affect the values observed for the dependent variable.

Lastly but importantly, a solid hypothesis often carries potential implications for future research areas and can potentially lead to further tests and studies if verified.

Elements that make up a well-formulated hypothesis

Delving deeper into what shapes up a robust hypothesis, we realize that certain crucial components determine its effectiveness.

Firstly, every good hypothesis or test has clear variables which essentially refer to specific aspects of the study subject matter being measured or manipulated during research. These aspects are segregated as:

  • Independent Variable (IV): This component relates directly to what you have control over in your study.
  • Dependent Variable (DV): This component consists of outcomes affected by alterations made in IV

Next comes ‘Predicted Outcome’ – what you anticipate happening as repercussions due to modification of two or more variables under scrutiny.

The ‘Testability’ factor also holds veritable importance comprising experimental procedures capable enough to refute or accept your claims.

The last element circles the argument around presenting a capacity called ‘Relationship’ correlating IV with DV believed to either causing some effect or showcasing an association.

Hence, these prime facets further accentuate your endeavor to adequately define the hypothesis.

Importance and Purpose of a Hypothesis

Understanding the Role of a Hypothesis in Research

First, let’s delve into the overarching role that hypothesis plays within research scenarios. As we define the hypothesis, you should view this as an underlying pillar or guiding star for your investigation. A well-articulated hypothesis steers your exploration by providing clarity on what specifically you aim to examine.

A meaningful analogy would be considering a hypothesis as a compass during a voyage. If research is the vast ocean where confusing whirlpools of data and evidence abound, then it can guide us in our direction rather than letting us drift aimlessly. Furthermore, the formulation of a quality hypothesis inherently demands clarity about your objectives upfront – this essentially sets your research vessel on course bearing towards effective outcomes.

Exploring Why Formulating A Hypothesis is Crucial in Scientific Investigations

So why precisely is nurturing such a detailed forecast vital?

  • Structural Advantage: By proposing potential answers to posed questions via hypotheses, researchers streamline their methods and techniques. The approach undertaken depends significantly on what the suggested outcome or phenomenon might be.
  • Generate Preliminary Expectations: Even if they’re proven wrong, making observations and developing models based on hypotheses often lead to more interesting inquiries or turn up unexpected findings.
  • Quantifiable Predictions: More than simple conjectures, strong hypotheses are testable; they propose results expressed in measurable terms.

In essence, remember that formulating hypotheses smoothes the path towards solid conclusions by being the architect’s blueprints of robust investigations. Never underestimate the forward thrust they provide for progress within scientific inquiry!

Types of Hypotheses

Once we understand to define a hypothesis, we’ll find that hypotheses come in several types. Different classifications of plural hypotheses depend on their formulations and the nature of predictions or assumptions they lead towards – simple, complex, directional, non-directional, null, associative and causal. Let’s explore some of these.

Simple Hypothesis: Definition and Examples

A simple hypothesis is a type of prediction or an educated guess that carries one independent variable and one dependent variable. In essence, it creates a relationship between two singular entities; for instance, ‘Exercise improves memory.’ This suggests that there’s an impact (of improvement) on the ‘memory’ (dependent variable) by ‘exercise’ (independent variable).

Complex Hypothesis: Definition and Examples

On the contrary to its name mate – a simple hypothesis – a complex hypothesis involves more than just two variables. It points out multiple variables and how they interlink with each other. The effects aren’t just limited to cause-and-effect but can be interactive or combined impact-dependent variables too – for instance,’Diet and exercise affect weight loss and heart health.’ Here, diet and exercise are your independent factors influencing multifold aspects like weight loss (a dependent variable) alongside heart health(another dependent variable).

Directional Hypothesis: Definition and Examples

One might argue that the path laid by a directional hypothesis is less twisted as it predicts the directionality of an effect – whether one variable will increase or decrease another variable. An example here could be “Cutting down on alcohol will reduce liver disorders.” Here a reduction in ‘drinking alcohol’ implicitly identifies fewer occurrences of ‘liver disorders.’

Non-directional Hypothesis: Definition and Examples

Sometimes science requires open-ended answers; henceforth comes into play our non-directional hypothesis which merely stipulates that there’s going to be an impact without specifying its course – good, bad or otherwise. For example, “Exposure to secondhand smoke influences lung health.” It infers that there’s an effect on ‘lung health’ due to ‘secondhand smoke,’ without indicating if it’s an improvement or deterioration.

Null Hypothesis: Definition and Examples

The null hypothesis, often symbolized as H0, makes things pretty straight with assumptions; basically, it purports no existence of a relationship between the variables. Researchers utilize this hypothesis chiefly for statistical testing. In lay terms – “Smoking is not linked to lung cancer.” Here a nonexistence of association is suggested between ‘smoking’ and ‘lung cancer.’

Associative and Causal Hypothesis: Explanation and Examples

Now leaving the train station named Null-ville we enter into quite associative terrain where the associative hypothesis foretells ‘relationships’ but are shy when it comes to cause-effects. An instance could be “Students scoring high also tend to play chess.” These fellows here don’t claim that playing chess outrightly shoots up scores yet suggests a specific pattern.

On another spectrum brightful cause-effect claims jump in bravely shouting out not just relationships but boldly stating their causes too – “Consumption of fast food leads to obesity” is being so certain about fast food consumption (cause) escalating obesity levels(effect).

Navigating through these alternative hypotheses and variants allows us to step into researchers’ shoes better while also helps defining complex constructions bit by bit, making them simple outcomes anyone can interpret.

Developing and Testing a Hypothesis

In the world of research, it’s not uncommon to hear someone say “Let’s define hypothesis!” This term may seem complex at first glance, but its essence falls within our natural instinct to question and learn. To give structure to this innate curiosity, we form hypotheses and navigate through the rigorous process of testing them.

Process of Formulating a Hypothesis

Forming an effective hypothesis is both an art and a science. It involves finding a perfect blend between creativity and logical reasoning. Here are some simple yet essential steps you’d want to follow:

  • Identify Your Research Question – The first step towards formulating a hypothesis is defining your research question based on preliminary observations or literature review.
  • Conduct Thorough Literature Review – Once your question is in place, an extensive read about what has already been studied can help refine it further.
  • Create Tentative Explanation – Develop a preliminary answer based on your knowledge and understanding which will serve as your tentative explanation or hypothesis.
  • Refine Your Hypothesis : Refine this initial guess considering available resources for empirical testing, ethical implications, and potential outcomes.

Remember that the key is formation clarity in statement-making; overly complex language might obscure rather than clarify your central idea.

Importance of Testing a Hypothesis Through Empirical Research Methods

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Testing a hypothesis isn’t simply about proving it right or wrong; it’s much more refined than that – it’s about validation and advancement of human knowledge. By applying empirical methods such as observation or experimentation, logic meets practice in real-world scenarios.

These hands-on approaches afford us precious insights into how our theories hold up under scrutiny outside the confines of abstract thought alone.

  • Validity Confirmation : Empirical testing helps confirm if our predictions were correct or not, providing validation for our presumptions.
  • Understanding Relationships : Testing allows us to assess the relational dynamics between variables under investigation.
  • Promotes Scientific Inquiry : Empirical testing encourages a systematic and objective approach to understanding phenomena, which lies at the heart of scientific inquiry.

Consider this: hypotheses are our best-educated guesses – smart hunches rooted in what we know so far. To move beyond guessing and into knowledgeable assertion, we define hypothesis structure as one that can be empirically tested. Only then do we truly start to shape our understanding with any level of certainty.

Examples of Hypotheses in Different Fields

Indeed, it’s fundamental to understand that hypotheses are not confined to a single discipline but span across numerous fields. To better illuminate this, let’s delve into various examples.

Examples of Hypotheses in Scientific Research Studies

In the realm of scientific research studies, hypotheses play a pivotal role in shaping the basis for investigations research hypotheses and experiments. Let’s consider an elementary example: studying plant growth. A researcher might formulate the hypothesis – “If a specific type of fertilizer is used, then plants will grow more rapidly.” This hypothesis aims to validate or refute the assumption that given fertilizer perceptibly affects plant growth rate.

Another common example arises from investigating causal relationships between physical activity and heart health. The scientist may hypothesize that “Regular aerobic exercise decreases the risk of heart disease.”

Examples of Hypotheses in Social Sciences

When we transition towards social sciences, which deals with human behavior and its relation to societal constructs, our formative definitions undergo a change as well.

Imagine researchers examining how socioeconomic status influences educational attainment rates. They could pose a hypothesis saying, “High socioeconomic status positively correlates with higher levels of formal education.” This hypothesis attempts to tie economic background directly to education outcomes.

The correlation between gender diversity within workplace teams and improved business performance presents another illustration. A possible hypothesis could be – “Teams comprising diverse genders exhibit superior business performance than homogenous teams.”

Examples of Hypotheses in Psychology

Within psychology – the study dedicated to how individuals think, feel, and behave; clearly stated hypotheses serve as essential stepping stones for meaningful findings and insights.

Take, for instance, predicting performance under pressure: psychologists may propose an assumption like – “Stress triggers increased errors on complex tasks”. Or when researching cognitive development in children – they may hypothesize – “Language acquisition accelerates once children start attending school”.

Examples of Hypotheses in Medical Research

Lastly but importantly, in medical research, well-articulated hypotheses help probe pressing healthcare questions and identify effective treatments.

For instance: “Patients receiving chemotherapy experience significant weight loss”. Or regarding disease transmission during pandemics – they might propose “Regular hand sanitation reduces the risk of COVID-19 infection.”

In conclusion, these examples hopefully underline the importance and versatility of a hypothesis in scientific inquiry. Irrespective of its utilization within various research fields, a scientific hypothesis still essentially remains an educated assumption that offers direction and purpose to the investigation. Interestingly enough, each study’s defined hypothesis sets forth a path leading towards a better comprehension of our world and life within it.

Common Mistakes to Avoid when Formulating a Hypothesis

Identifying errors that researchers often make when developing a hypothesis.

Many researchers, especially those new in the field, may sometimes falter while crafting their hypotheses. Here are some frequently observed mistakes:

  • Framing Vague Hypotheses : Clarity is vital when defining your hypothesis. A common pitfall involves creating an ambiguous statement which leaves room for multiple interpretations. This hinders precise data collection and analysis.
  • Formulating Unfalsifiable Hypotheses : These are statements that cannot be proven false because they don’t connect to observable or measurable variables.
  • Targeting Unachievable Results : Often, there is an inclination to develop complex hypotheses expecting groundbreaking findings. However, it’s crucial to limit the scope according to practical constraints and possibilities.
  • Ignoring Null Hypothesis : The null hypothesis provides a means of contradiction to the alternative hypothesis being tested, making it essential for any research study.

Tips for avoiding these mistakes

After identifying the commonly made errors when forming a hypothesis, let’s now consider some proactive measures you can adopt:

  • Crystallize Your Thoughts : Before you articulate your hypothesis, refine and clarify your ideas first. Define the parameters of your study clearly and ensure your proposition directly aligns with them.
  • Keep It Simple : Stick with simplicity as much as possible in describing expected relationships or patterns in your research subject area. Remember: A simpler hypothesis often leads to effective testing.
  • Embrace Falsifiability . To avoid making unfalsifiable claims, learn how to craft ‘If – Then’ statements articulately in your define hypothesis process.
  • Remember the Null Hypothesis : Always formulate and account for a null hypothesis—a statement that negates the relationship between variables—for robust results validation.

In truth, it takes practice to strike the right balance and formulate a solid, practical hypothesis for your research. With these tips in mind, you’re better equipped to avoid common pitfalls that can compromise the quality of your investigation as they guide your approach when you define hypotheses.

Evaluating and Refining a Hypothesis

Laying out a hypothesis is merely the first stage of an intricate journey. Testing and refining this conjecture is equally pivotal in perfecting your next scientific method of undertaking. This pathway comprises evaluation for validity, and relevance, followed by refinement through research findings.

Methods for Assessing the Validity and Relevance of a Hypothesis

To define a hypothesis of meticulosity, we need to subject it to rigorous scrutiny. Utilizing statistical tests enables you to judge the validity of your hypothesis. Here’s a brief look at some key methods that can assist in assessing your theory:

  • Empirical Testing : Conduct experiments or surveys as per the requirements of your study.
  • Consistency Check : The hypothesis should remain consistent with other established theories and laws within its field.
  • Falsifiability principle : Proposed by Karl Popper, a valid hypothesis must be capable of being proven wrong.

Let me reemphasize here, that relevance plays an integral part too especially when defining hypotheses linked with pragmatics like social sciences or business studies.

A relevant hypothesis will hold significance to not just existing knowledge but also pave the way for future work within the particular area of expertise. It should address gaps in current scientific theories while shedding light on possible solutions.

Ways to Refine and Modify a Hypothesis Based on Research Findings

Our job doesn’t end up on developing an initial proposition; it’s crucial to use findings from our research to refine that preliminary conception further. This essential process breathes life into what was once purely speculative.

While refining your conjecture can sound daunting initially, I assure you it’s nothing more complicated than diagnosing any missing links between your original theory and novel evidence you’ve discovered along this research journey.

If H0 (null hypothesis) contradicts your empirical results, then getting back onto the drafting board becomes necessary for crafting H1 (alternative hypothesis). This scientific cycle of formulating, testing then reformulating the hypotheses can continue till we eventually reach statistically significant results.

Remember, it’s important to be open-minded and responsive towards indications from your research findings. They will guide you intuitively in tweaking your working hypothesis in sync with your target goals.

Hence we must embrace this intricate art of defining a hypothesis while simultaneously embracing its dynamic nature which requires periodic refinement based upon insightful feedback from meticulous research.

Summarizing the Key Points About the Definition and Characteristics of a Hypothesis

Having delved into the concept extensively, we can confidently define a hypothesis as an informed and testable guess or prediction that acts as a guiding light in research studies and scientific investigations. When formulated correctly, it comprises two essential elements: clarity and specificity. It should be free from ambiguity, allowing other researchers to easily understand its proposed idea and the direction the study is heading.

In addition, a robust hypothesis exhibits predictability. As a researcher, you’re not only stating what you think will happen but also defining the variables in your experiment – your assumption confines your investigation’s parameters to make it manageable. Lastly, remember that any meaningful hypothesis must be verifiable — capable of being supported or refuted through data collection and analysis.

Reiterating the Importance of Hypotheses in Scientific Inquiry and Research

This discourse wouldn’t be complete without reaffirming how indispensable hypotheses are within scientific explorations and research inquiries. A conceptualized hypothesis serves as a foundational block upon which every aspect of a research project is built. It directs your observations along assumed patterns, thereby saving time during investigations.

We also need to note that formulating hypotheses promotes critical thinking skills among researchers because they require logical reasoning backed by empirical evidence rather than just empty conjectures.

Henceforth, whether you’re treading through unchartered waters of complex scientific endeavors or conducting social science research with less strict rules for predictions – keeping these insights on “define hypothesis” at hand would surely enhance your journey towards revealing valuable truths.

In essence, cultivating a comprehensive understanding of what constitutes a well-formed hypothesis not only lends credibility to our investigative ventures but also enables us to bring precision, focus, and relevance to our chosen field of exploration. The power lies in its simplistic yet profound ability to guide us from uncertainty towards concrete evidential findings – truly embodying scientific inquiry’s spirit!

Unlock the Power of Visualization with Mind the Graph: Elevate Your Hypothesis to New Heights

As a scientist, your hypothesis is the cornerstone of your research journey. But what if you could take it beyond mere words and equations, and transform it into a visual masterpiece that captivates your audience? Enter Mind the Graph , your ultimate ally in scientific visualization. With our intuitive platform, you can seamlessly translate complex hypotheses into stunning graphs, charts, and illustrations that speak volumes. Whether you are presenting at a conference, publishing a paper, or simply sharing your findings with the world, Mind the Graph empowers you to convey your hypotheses with clarity, precision, and undeniable impact. Join the scientific revolution today and let your hypotheses shine like never before with Mind the Graph.

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What Is Hypothesis Testing?

  • How It Works

4 Step Process

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Hypothesis Testing: 4 Steps and Example

key hypothesis meaning

Hypothesis testing, sometimes called significance testing, is an act in statistics whereby an analyst tests an assumption regarding a population parameter. The methodology employed by the analyst depends on the nature of the data used and the reason for the analysis.

Hypothesis testing is used to assess the plausibility of a hypothesis by using sample data. Such data may come from a larger population or a data-generating process. The word "population" will be used for both of these cases in the following descriptions.

Key Takeaways

  • Hypothesis testing is used to assess the plausibility of a hypothesis by using sample data.
  • The test provides evidence concerning the plausibility of the hypothesis, given the data.
  • Statistical analysts test a hypothesis by measuring and examining a random sample of the population being analyzed.
  • The four steps of hypothesis testing include stating the hypotheses, formulating an analysis plan, analyzing the sample data, and analyzing the result.

How Hypothesis Testing Works

In hypothesis testing, an  analyst  tests a statistical sample, intending to provide evidence on the plausibility of the null hypothesis. Statistical analysts measure and examine a random sample of the population being analyzed. All analysts use a random population sample to test two different hypotheses: the null hypothesis and the alternative hypothesis.

The null hypothesis is usually a hypothesis of equality between population parameters; e.g., a null hypothesis may state that the population mean return is equal to zero. The alternative hypothesis is effectively the opposite of a null hypothesis. Thus, they are mutually exclusive , and only one can be true. However, one of the two hypotheses will always be true.

The null hypothesis is a statement about a population parameter, such as the population mean, that is assumed to be true.

  • State the hypotheses.
  • Formulate an analysis plan, which outlines how the data will be evaluated.
  • Carry out the plan and analyze the sample data.
  • Analyze the results and either reject the null hypothesis, or state that the null hypothesis is plausible, given the data.

Example of Hypothesis Testing

If an individual wants to test that a penny has exactly a 50% chance of landing on heads, the null hypothesis would be that 50% is correct, and the alternative hypothesis would be that 50% is not correct. Mathematically, the null hypothesis is represented as Ho: P = 0.5. The alternative hypothesis is shown as "Ha" and is identical to the null hypothesis, except with the equal sign struck-through, meaning that it does not equal 50%.

A random sample of 100 coin flips is taken, and the null hypothesis is tested. If it is found that the 100 coin flips were distributed as 40 heads and 60 tails, the analyst would assume that a penny does not have a 50% chance of landing on heads and would reject the null hypothesis and accept the alternative hypothesis.

If there were 48 heads and 52 tails, then it is plausible that the coin could be fair and still produce such a result. In cases such as this where the null hypothesis is "accepted," the analyst states that the difference between the expected results (50 heads and 50 tails) and the observed results (48 heads and 52 tails) is "explainable by chance alone."

When Did Hypothesis Testing Begin?

Some statisticians attribute the first hypothesis tests to satirical writer John Arbuthnot in 1710, who studied male and female births in England after observing that in nearly every year, male births exceeded female births by a slight proportion. Arbuthnot calculated that the probability of this happening by chance was small, and therefore it was due to “divine providence.”

What are the Benefits of Hypothesis Testing?

Hypothesis testing helps assess the accuracy of new ideas or theories by testing them against data. This allows researchers to determine whether the evidence supports their hypothesis, helping to avoid false claims and conclusions. Hypothesis testing also provides a framework for decision-making based on data rather than personal opinions or biases. By relying on statistical analysis, hypothesis testing helps to reduce the effects of chance and confounding variables, providing a robust framework for making informed conclusions.

What are the Limitations of Hypothesis Testing?

Hypothesis testing relies exclusively on data and doesn’t provide a comprehensive understanding of the subject being studied. Additionally, the accuracy of the results depends on the quality of the available data and the statistical methods used. Inaccurate data or inappropriate hypothesis formulation may lead to incorrect conclusions or failed tests. Hypothesis testing can also lead to errors, such as analysts either accepting or rejecting a null hypothesis when they shouldn’t have. These errors may result in false conclusions or missed opportunities to identify significant patterns or relationships in the data.

Hypothesis testing refers to a statistical process that helps researchers determine the reliability of a study. By using a well-formulated hypothesis and set of statistical tests, individuals or businesses can make inferences about the population that they are studying and draw conclusions based on the data presented. All hypothesis testing methods have the same four-step process, which includes stating the hypotheses, formulating an analysis plan, analyzing the sample data, and analyzing the result.

Sage. " Introduction to Hypothesis Testing ," Page 4.

Elder Research. " Who Invented the Null Hypothesis? "

Formplus. " Hypothesis Testing: Definition, Uses, Limitations and Examples ."

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What is Hypothesis?

We have heard of many hypotheses which have led to great inventions in science. Assumptions that are made on the basis of some evidence are known as hypotheses. In this article, let us learn in detail about the hypothesis and the type of hypothesis with examples.

A hypothesis is an assumption that is made based on some evidence. This is the initial point of any investigation that translates the research questions into predictions. It includes components like variables, population and the relation between the variables. A research hypothesis is a hypothesis that is used to test the relationship between two or more variables.

Characteristics of Hypothesis

Following are the characteristics of the hypothesis:

  • The hypothesis should be clear and precise to consider it to be reliable.
  • If the hypothesis is a relational hypothesis, then it should be stating the relationship between variables.
  • The hypothesis must be specific and should have scope for conducting more tests.
  • The way of explanation of the hypothesis must be very simple and it should also be understood that the simplicity of the hypothesis is not related to its significance.

Sources of Hypothesis

Following are the sources of hypothesis:

  • The resemblance between the phenomenon.
  • Observations from past studies, present-day experiences and from the competitors.
  • Scientific theories.
  • General patterns that influence the thinking process of people.

Types of Hypothesis

There are six forms of hypothesis and they are:

  • Simple hypothesis
  • Complex hypothesis
  • Directional hypothesis
  • Non-directional hypothesis
  • Null hypothesis
  • Associative and casual hypothesis

Simple Hypothesis

It shows a relationship between one dependent variable and a single independent variable. For example – If you eat more vegetables, you will lose weight faster. Here, eating more vegetables is an independent variable, while losing weight is the dependent variable.

Complex Hypothesis

It shows the relationship between two or more dependent variables and two or more independent variables. Eating more vegetables and fruits leads to weight loss, glowing skin, and reduces the risk of many diseases such as heart disease.

Directional Hypothesis

It shows how a researcher is intellectual and committed to a particular outcome. The relationship between the variables can also predict its nature. For example- children aged four years eating proper food over a five-year period are having higher IQ levels than children not having a proper meal. This shows the effect and direction of the effect.

Non-directional Hypothesis

It is used when there is no theory involved. It is a statement that a relationship exists between two variables, without predicting the exact nature (direction) of the relationship.

Null Hypothesis

It provides a statement which is contrary to the hypothesis. It’s a negative statement, and there is no relationship between independent and dependent variables. The symbol is denoted by “H O ”.

Associative and Causal Hypothesis

Associative hypothesis occurs when there is a change in one variable resulting in a change in the other variable. Whereas, the causal hypothesis proposes a cause and effect interaction between two or more variables.

Examples of Hypothesis

Following are the examples of hypotheses based on their types:

  • Consumption of sugary drinks every day leads to obesity is an example of a simple hypothesis.
  • All lilies have the same number of petals is an example of a null hypothesis.
  • If a person gets 7 hours of sleep, then he will feel less fatigue than if he sleeps less. It is an example of a directional hypothesis.

Functions of Hypothesis

Following are the functions performed by the hypothesis:

  • Hypothesis helps in making an observation and experiments possible.
  • It becomes the start point for the investigation.
  • Hypothesis helps in verifying the observations.
  • It helps in directing the inquiries in the right direction.

How will Hypothesis help in the Scientific Method?

Researchers use hypotheses to put down their thoughts directing how the experiment would take place. Following are the steps that are involved in the scientific method:

  • Formation of question
  • Doing background research
  • Creation of hypothesis
  • Designing an experiment
  • Collection of data
  • Result analysis
  • Summarizing the experiment
  • Communicating the results

Frequently Asked Questions – FAQs

What is hypothesis.

A hypothesis is an assumption made based on some evidence.

Give an example of simple hypothesis?

What are the types of hypothesis.

Types of hypothesis are:

  • Associative and Casual hypothesis

State true or false: Hypothesis is the initial point of any investigation that translates the research questions into a prediction.

Define complex hypothesis..

A complex hypothesis shows the relationship between two or more dependent variables and two or more independent variables.

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  1. Lock-and-key model Definition and Examples

    Lock-and-key model Definition. Lock-and-key model is a model for enzyme-substrate interaction suggesting that the enzyme and the substrate possess specific complementary geometric shapes that fit exactly into one another. In this model, enzymes are depicted as highly specific. They must bind to specific substrates before they catalyze chemical reactions. ...

  2. Lock and Key Model- Mode of Action of Enzymes

    A German scientist, Emil Fischer postulated the lock and key model in 1894 to explain the enzyme's mode of action. Fischer's theory hypothesized that enzymes exhibit a high degree of specificity towards the substrate. This model assumes that the active site of the enzyme and the substrate fit perfectly into one another such that each ...

  3. What are enzymes?

    In the lock and key hypothesis close lock and key hypothesis Model which compares the specificity of enzymes with a key and its lock., the shape of the active site matches the shape of its ...

  4. The scientific method (article)

    The scientific method. At the core of biology and other sciences lies a problem-solving approach called the scientific method. The scientific method has five basic steps, plus one feedback step: Make an observation. Ask a question. Form a hypothesis, or testable explanation. Make a prediction based on the hypothesis.

  5. What Is A Research Hypothesis? A Simple Definition

    A research hypothesis (also called a scientific hypothesis) is a statement about the expected outcome of a study (for example, a dissertation or thesis). To constitute a quality hypothesis, the statement needs to have three attributes - specificity, clarity and testability. Let's take a look at these more closely.

  6. Scientific hypothesis

    Scientific hypothesis, idea that proposes an explanation for an observed phenomenon or narrow set of phenomena. Two key features of a scientific hypothesis are falsifiability and testability, which are reflected in an 'If...then' statement, and the ability to be supported or refuted in observation or experimentation.

  7. Molecular Recognition: Lock-and-Key, Induced Fit, and ...

    In 1894, Emil Fisher discovered that glycolytic enzymes are able to distinguish between sugar stereoisomers. Based upon that discovery, he formulated the lock-and-key hypothesis (Fischer 1894), which proposed that enzymes recognize their substrates just as a lock receives a key.That is, only in the case of exact geometric complementarity between the substrate (key) and enzyme (lock) is the ...

  8. On the scope of scientific hypotheses

    2. The scientific hypothesis. In this section, we will describe a functional and descriptive role regarding how scientists use hypotheses. Jeong & Kwon [] investigated and summarized the different uses the concept of 'hypothesis' had in philosophical and scientific texts.They identified five meanings: assumption, tentative explanation, tentative cause, tentative law, and prediction.

  9. Enzymes

    The Lock-and-key Hypothesis. The Lock-and-key Hypothesis is a model of how Enzymes catalyse Substrate reactions. It states that the shape of the Active Sites of Enzymes are exactly Complementary to the shape of the Substrate. When a substrate molecule collides with an enzyme whose Active Site shape is complementary, ...

  10. Lock-and-key theory

    Search for: 'lock-and-key theory' in Oxford Reference ». A theory to explain the mechanism of enzymatic reactions, in which it is proposed that the enzyme and substrate (s) bind temporarily to form an enzyme-substrate complex. The binding site on the enzyme is known as the 'active site' and is structurally complementary to the substrate (s).

  11. What is a Hypothesis

    Definition: Hypothesis is an educated guess or proposed explanation for a phenomenon, based on some initial observations or data. It is a tentative statement that can be tested and potentially proven or disproven through further investigation and experimentation. Hypothesis is often used in scientific research to guide the design of experiments ...

  12. What is a scientific hypothesis?

    A scientific hypothesis is a tentative, testable explanation for a phenomenon in the natural world. It's the initial building block in the scientific method. Many describe it as an "educated guess ...

  13. What is a Research Hypothesis: How to Write it, Types, and Examples

    It seeks to explore and understand a particular aspect of the research subject. In contrast, a research hypothesis is a specific statement or prediction that suggests an expected relationship between variables. It is formulated based on existing knowledge or theories and guides the research design and data analysis. 7.

  14. Hypothesis Definition & Meaning

    hypothesis: [noun] an assumption or concession made for the sake of argument. an interpretation of a practical situation or condition taken as the ground for action.

  15. Hypothesis: Definition, Examples, and Types

    A hypothesis is a tentative statement about the relationship between two or more variables. It is a specific, testable prediction about what you expect to happen in a study. It is a preliminary answer to your question that helps guide the research process. Consider a study designed to examine the relationship between sleep deprivation and test ...

  16. How to Write a Strong Hypothesis

    Developing a hypothesis (with example) Step 1. Ask a question. Writing a hypothesis begins with a research question that you want to answer. The question should be focused, specific, and researchable within the constraints of your project. Example: Research question.

  17. 9.1: Introduction to Hypothesis Testing

    In hypothesis testing, the goal is to see if there is sufficient statistical evidence to reject a presumed null hypothesis in favor of a conjectured alternative hypothesis.The null hypothesis is usually denoted \(H_0\) while the alternative hypothesis is usually denoted \(H_1\). An hypothesis test is a statistical decision; the conclusion will either be to reject the null hypothesis in favor ...

  18. Hypothesis Testing

    Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is most often used by scientists to test specific predictions, called hypotheses, that arise from theories. ... Stating results in a statistics assignment In our comparison of mean height between men and women we found an average difference ...

  19. Define Hypothesis: Unveiling the First Step in Scientific Inquiry

    Summarizing the Key Points About the Definition and Characteristics of a Hypothesis. Having delved into the concept extensively, we can confidently define a hypothesis as an informed and testable guess or prediction that acts as a guiding light in research studies and scientific investigations.

  20. Hypothesis Testing: 4 Steps and Example

    Hypothesis testing is an act in statistics whereby an analyst tests an assumption regarding a population parameter. The methodology employed by the analyst depends on the nature of the data used ...

  21. What is Hypothesis

    Functions of Hypothesis. Following are the functions performed by the hypothesis: Hypothesis helps in making an observation and experiments possible. It becomes the start point for the investigation. Hypothesis helps in verifying the observations. It helps in directing the inquiries in the right direction.